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Protein

Peroxiredoxin-4

Gene

Prdx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events (PubMed:11229364). Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation (By similarity).By similarity1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • protein homodimerization activity Source: MGI
  • thioredoxin peroxidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Protein family/group databases

PeroxiBasei4532 Mm2CysPrx04

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-4 (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme AOE372
Peroxiredoxin IV
Short name:
Prx-IV
Thioredoxin peroxidase AO372
Thioredoxin-dependent peroxide reductase A0372
Gene namesi
Name:Prdx4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1859815 Prdx4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40By similarityAdd BLAST40
ChainiPRO_000013509941 – 274Peroxiredoxin-4Add BLAST234

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi127Interchain (with C-248); in linked formCombined sources1 Publication
Disulfide bondi248Interchain (with C-127); in linked formCombined sources1 Publication

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiO08807
MaxQBiO08807
PaxDbiO08807
PeptideAtlasiO08807
PRIDEiO08807

2D gel databases

REPRODUCTION-2DPAGEiO08807

PTM databases

iPTMnetiO08807
PhosphoSitePlusiO08807
SwissPalmiO08807

Expressioni

Gene expression databases

BgeeiENSMUSG00000025289 Expressed in 284 organ(s), highest expression level in ear vesicle
CleanExiMM_PRDX4
ExpressionAtlasiO08807 baseline and differential
GenevisibleiO08807 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TXNDC5Q8NBS9-12EBI-494652,EBI-16091651From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207303, 3 interactors
DIPiDIP-34137N
IntActiO08807, 12 interactors
MINTiO08807
STRINGi10090.ENSMUSP00000026328

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO08807
SMRiO08807
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 240ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
HOVERGENiHBG000286
InParanoidiO08807
KOiK03386
OrthoDBiEOG091G0IE5
PhylomeDBiO08807
TreeFamiTF105181

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O08807-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEARSKLLDG TTASRRWTRK LVLLLPPLLL FLLRTESLQG LESDERFRTR
60 70 80 90 100
ENECHFYAGG QVYPGEASRV SVADHSLHLS KAKISKPAPY WEGTAVINGE
110 120 130 140 150
FKELKLTDYR GKYLVFFFYP LDFTFVCPTE IIAFGDRIEE FKSINTEVVA
160 170 180 190 200
CSVDSQFTHL AWINTPRRQG GLGPIRIPLL SDLNHQISKD YGVYLEDSGH
210 220 230 240 250
TLRGLFIIDD KGVLRQITLN DLPVGRSVDE TLRLVQAFQY TDKHGEVCPA
260 270
GWKPGSETII PDPAGKLKYF DKLN
Length:274
Mass (Da):31,053
Last modified:July 1, 1997 - v1
Checksum:i73DB5374EC46241C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AZS9B1AZS9_MOUSE
Peroxiredoxin-4
Prdx4
229Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96746 mRNA Translation: AAB57846.1
AK005031 mRNA Translation: BAB23758.1
AK146402 mRNA Translation: BAE27143.1
AK152255 mRNA Translation: BAE31074.1
BX005263 Genomic DNA No translation available.
BC003349 mRNA Translation: AAH03349.1
BC019578 mRNA Translation: AAH19578.1
CCDSiCCDS30496.1
RefSeqiNP_001300640.1, NM_001313711.1
NP_058044.1, NM_016764.5
UniGeneiMm.247542

Genome annotation databases

EnsembliENSMUST00000026328; ENSMUSP00000026328; ENSMUSG00000025289
GeneIDi53381
KEGGimmu:53381
UCSCiuc009uru.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96746 mRNA Translation: AAB57846.1
AK005031 mRNA Translation: BAB23758.1
AK146402 mRNA Translation: BAE27143.1
AK152255 mRNA Translation: BAE31074.1
BX005263 Genomic DNA No translation available.
BC003349 mRNA Translation: AAH03349.1
BC019578 mRNA Translation: AAH19578.1
CCDSiCCDS30496.1
RefSeqiNP_001300640.1, NM_001313711.1
NP_058044.1, NM_016764.5
UniGeneiMm.247542

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VWUX-ray3.30A/B/C/D/E/F/G/H/I/J41-274[»]
3VWVX-ray1.80A/B87-274[»]
3W8JX-ray2.10C/D244-263[»]
3WGXX-ray0.92C/D244-263[»]
ProteinModelPortaliO08807
SMRiO08807
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207303, 3 interactors
DIPiDIP-34137N
IntActiO08807, 12 interactors
MINTiO08807
STRINGi10090.ENSMUSP00000026328

Protein family/group databases

PeroxiBasei4532 Mm2CysPrx04

PTM databases

iPTMnetiO08807
PhosphoSitePlusiO08807
SwissPalmiO08807

2D gel databases

REPRODUCTION-2DPAGEiO08807

Proteomic databases

EPDiO08807
MaxQBiO08807
PaxDbiO08807
PeptideAtlasiO08807
PRIDEiO08807

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026328; ENSMUSP00000026328; ENSMUSG00000025289
GeneIDi53381
KEGGimmu:53381
UCSCiuc009uru.1 mouse

Organism-specific databases

CTDi10549
MGIiMGI:1859815 Prdx4

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
HOVERGENiHBG000286
InParanoidiO08807
KOiK03386
OrthoDBiEOG091G0IE5
PhylomeDBiO08807
TreeFamiTF105181

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:O08807
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025289 Expressed in 284 organ(s), highest expression level in ear vesicle
CleanExiMM_PRDX4
ExpressionAtlasiO08807 baseline and differential
GenevisibleiO08807 MM

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRDX4_MOUSE
AccessioniPrimary (citable) accession number: O08807
Secondary accession number(s): B1AZS7, Q3U8E4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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