UniProtKB - O08796 (EF2K_MOUSE)
Protein
Eukaryotic elongation factor 2 kinase
Gene
Eef2k
Organism
Mus musculus (Mouse)
Status
Functioni
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.1 Publication
Catalytic activityi
- [translation elongation factor 2] + ATP = [translation elongation factor 2]-phosphate + ADP + H+1 PublicationEC:2.7.11.201 Publication
Activity regulationi
Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 295 – 301 | ATPBy similarity | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium ion binding Source: InterPro
- calmodulin binding Source: UniProtKB
- elongation factor-2 kinase activity Source: UniProtKB
- translation factor activity, RNA binding Source: UniProtKB
GO - Biological processi
- cellular response to anoxia Source: Ensembl
- cellular response to brain-derived neurotrophic factor stimulus Source: Ensembl
- cellular response to calcium ion Source: Ensembl
- cellular response to cAMP Source: Ensembl
- cellular response to insulin stimulus Source: Ensembl
- negative regulation of apoptotic process Source: MGI
- positive regulation of dendritic spine morphogenesis Source: MGI
- positive regulation of endocytosis Source: MGI
- positive regulation of synapse assembly Source: MGI
- protein autophosphorylation Source: MGI
- regulation of protein autophosphorylation Source: UniProtKB
- response to ischemia Source: Ensembl
- response to prolactin Source: Ensembl
- translational elongation Source: UniProtKB
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Calcium, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.20, 3474 |
Reactomei | R-MMU-166208, mTORC1-mediated signalling |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic elongation factor 2 kinase (EC:2.7.11.201 Publication)Short name: eEF-2 kinase Short name: eEF-2K Alternative name(s): Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
Gene namesi | Name:Eef2k |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1195261, Eef2k |
Subcellular locationi
Other locations
- dendritic spine Source: MGI
- postsynaptic density Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000086937 | 2 – 724 | Eukaryotic elongation factor 2 kinaseAdd BLAST | 723 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 27 | PhosphoserineBy similarity | 1 | |
Modified residuei | 61 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 70 | PhosphoserineCombined sources | 1 | |
Modified residuei | 73 | PhosphoserineBy similarity | 1 | |
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 242 | PhosphoserineBy similarity | 1 | |
Modified residuei | 347 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 352 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 358 | Phosphoserine; by MAPK13 and CDK1By similarity | 1 | |
Modified residuei | 365 | Phosphoserine; by autocatalysis, RPS6KA1 and RPS6KB1By similarity | 1 | |
Modified residuei | 391 | PhosphoserineCombined sources | 1 | |
Modified residuei | 397 | Phosphoserine; by AMPKBy similarity | 1 | |
Modified residuei | 434 | PhosphoserineBy similarity | 1 | |
Modified residuei | 444 | PhosphoserineCombined sources | 1 | |
Modified residuei | 469 | PhosphoserineBy similarity | 1 | |
Modified residuei | 473 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 476 | PhosphoserineBy similarity | 1 | |
Modified residuei | 499 | Phosphoserine; by PKABy similarity | 1 |
Post-translational modificationi
Autophosphorylated at multiple residues, Thr-347 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-397 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-77 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-358 or RPS6KA1 and RPS6KB1 at Ser-365 instead decrease EEF2K activity and promote protein synthesis (By similarity).By similarity
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O08796 |
jPOSTi | O08796 |
MaxQBi | O08796 |
PaxDbi | O08796 |
PeptideAtlasi | O08796 |
PRIDEi | O08796 |
PTM databases
iPTMneti | O08796 |
PhosphoSitePlusi | O08796 |
Expressioni
Tissue specificityi
Ubiquitously expressed. Particularly abundant in skeletal muscle and heart.
Gene expression databases
Bgeei | ENSMUSG00000035064, Expressed in primary oocyte and 279 other tissues |
Genevisiblei | O08796, MM |
Interactioni
Subunit structurei
Monomer or homodimer (Probable).
Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity (By similarity).
By similarityCuratedGO - Molecular functioni
- calmodulin binding Source: UniProtKB
Protein-protein interaction databases
IntActi | O08796, 1 interactor |
STRINGi | 10090.ENSMUSP00000046595 |
Miscellaneous databases
RNActi | O08796, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 115 – 325 | Alpha-type protein kinasePROSITE-ProRule annotationAdd BLAST | 211 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 93 | Calmodulin-bindingBy similarityAdd BLAST | 14 |
Domaini
The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain (By similarity).By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QVA3, Eukaryota |
GeneTreei | ENSGT00940000157839 |
HOGENOMi | CLU_382143_0_0_1 |
InParanoidi | O08796 |
OMAi | HWPVFGD |
OrthoDBi | 741011at2759 |
PhylomeDBi | O08796 |
TreeFami | TF316085 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR017400, eEF-2K IPR011009, Kinase-like_dom_sf IPR004166, MHCK_EF2_kinase IPR006597, Sel1-like IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF02816, Alpha_kinase, 1 hit PF08238, Sel1, 4 hits |
PIRSFi | PIRSF038139, Elongation_factor_2_kinase, 1 hit |
SMARTi | View protein in SMART SM00811, Alpha_kinase, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51158, ALPHA_KINASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O08796-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ
60 70 80 90 100
NVSSKVQSYY SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE
110 120 130 140 150
FHLEDIATEH ATRHRYNAVT GEWLKDEVLI KMASQPFGRG AMRECFRTKK
160 170 180 190 200
LSNFLHAQQW KGASNYVAKR YIEPVDRSVY FEDVQLQMEA KLWGEDYNRH
210 220 230 240 250
KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS NSGFVRDDNI
260 270 280 290 300
RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG
310 320 330 340 350
VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR
360 370 380 390 400
GTEEKCGSPR IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT
410 420 430 440 450
PHSQKLDHLH WPVFGDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL
460 470 480 490 500
DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA
510 520 530 540 550
VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA
560 570 580 590 600
IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL
610 620 630 640 650
LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD
660 670 680 690 700
EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM
710 720
EAMKGRLANQ YYEKAEEAWA QMEE
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF7A9F2 | F7A9F2_MOUSE | Eukaryotic elongation factor 2 kina... | Eef2k | 140 | Annotation score: | ||
F6ZEU2 | F6ZEU2_MOUSE | Eukaryotic elongation factor 2 kina... | Eef2k | 185 | Annotation score: | ||
D3Z1Y3 | D3Z1Y3_MOUSE | Eukaryotic elongation factor 2 kina... | Eef2k | 38 | Annotation score: | ||
D3Z088 | D3Z088_MOUSE | Eukaryotic elongation factor 2 kina... | Eef2k | 164 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93848 mRNA Translation: AAB58271.1 BC003433 mRNA Translation: AAH03433.1 |
CCDSi | CCDS21796.1 |
RefSeqi | NP_001254639.1, NM_001267710.1 NP_001254640.1, NM_001267711.1 NP_031934.1, NM_007908.4 XP_006507405.1, XM_006507342.3 XP_006507406.1, XM_006507343.3 |
Genome annotation databases
Ensembli | ENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064 ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064 ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064 |
GeneIDi | 13631 |
KEGGi | mmu:13631 |
UCSCi | uc009jnb.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93848 mRNA Translation: AAB58271.1 BC003433 mRNA Translation: AAH03433.1 |
CCDSi | CCDS21796.1 |
RefSeqi | NP_001254639.1, NM_001267710.1 NP_001254640.1, NM_001267711.1 NP_031934.1, NM_007908.4 XP_006507405.1, XM_006507342.3 XP_006507406.1, XM_006507343.3 |
3D structure databases
SMRi | O08796 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08796, 1 interactor |
STRINGi | 10090.ENSMUSP00000046595 |
PTM databases
iPTMneti | O08796 |
PhosphoSitePlusi | O08796 |
Proteomic databases
EPDi | O08796 |
jPOSTi | O08796 |
MaxQBi | O08796 |
PaxDbi | O08796 |
PeptideAtlasi | O08796 |
PRIDEi | O08796 |
Protocols and materials databases
Antibodypediai | 25835, 425 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047875; ENSMUSP00000046595; ENSMUSG00000035064 ENSMUST00000106488; ENSMUSP00000102097; ENSMUSG00000035064 ENSMUST00000106489; ENSMUSP00000102098; ENSMUSG00000035064 |
GeneIDi | 13631 |
KEGGi | mmu:13631 |
UCSCi | uc009jnb.3, mouse |
Organism-specific databases
CTDi | 29904 |
MGIi | MGI:1195261, Eef2k |
Phylogenomic databases
eggNOGi | ENOG502QVA3, Eukaryota |
GeneTreei | ENSGT00940000157839 |
HOGENOMi | CLU_382143_0_0_1 |
InParanoidi | O08796 |
OMAi | HWPVFGD |
OrthoDBi | 741011at2759 |
PhylomeDBi | O08796 |
TreeFami | TF316085 |
Enzyme and pathway databases
BRENDAi | 2.7.11.20, 3474 |
Reactomei | R-MMU-166208, mTORC1-mediated signalling |
Miscellaneous databases
BioGRID-ORCSi | 13631, 1 hit in 17 CRISPR screens |
ChiTaRSi | Eef2k, mouse |
PROi | PR:O08796 |
RNActi | O08796, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035064, Expressed in primary oocyte and 279 other tissues |
Genevisiblei | O08796, MM |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR017400, eEF-2K IPR011009, Kinase-like_dom_sf IPR004166, MHCK_EF2_kinase IPR006597, Sel1-like IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF02816, Alpha_kinase, 1 hit PF08238, Sel1, 4 hits |
PIRSFi | PIRSF038139, Elongation_factor_2_kinase, 1 hit |
SMARTi | View protein in SMART SM00811, Alpha_kinase, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51158, ALPHA_KINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF2K_MOUSE | |
Accessioni | O08796Primary (citable) accession number: O08796 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index