UniProtKB - O08784 (TCOF_MOUSE)
Protein
Treacle protein
Gene
Tcof1
Organism
Mus musculus (Mouse)
Status
Functioni
Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein-macromolecule adaptor activity Source: MGI
- RNA polymerase I core binding Source: GO_Central
- scaffold protein binding Source: MGI
GO - Biological processi
- neural crest cell development Source: UniProtKB
- neural crest formation Source: UniProtKB
- nucleolar large rRNA transcription by RNA polymerase I Source: MGI
- regulation of translation Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Treacle proteinAlternative name(s): Treacher Collins syndrome protein homolog |
Gene namesi | Name:Tcof1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:892003, Tcof1 |
Subcellular locationi
Nucleus
- nucleolus By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- fibrillar center Source: MGI
- nucleolus Source: MGI
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072460 | 1 – 1320 | Treacle proteinAdd BLAST | 1320 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 83 | PhosphoserineBy similarity | 1 | |
Modified residuei | 85 | PhosphoserineBy similarity | 1 | |
Modified residuei | 87 | PhosphoserineBy similarity | 1 | |
Modified residuei | 88 | PhosphoserineBy similarity | 1 | |
Modified residuei | 104 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 126 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 132 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 151 | PhosphoserineBy similarity | 1 | |
Modified residuei | 153 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 154 | PhosphoserineCombined sources | 1 | |
Modified residuei | 169 | PhosphoserineCombined sources | 1 | |
Modified residuei | 171 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 301 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 318 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 324 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 327 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 386 | PhosphoserineBy similarity | 1 | |
Modified residuei | 413 | PhosphoserineCombined sources | 1 | |
Modified residuei | 414 | PhosphoserineCombined sources | 1 | |
Modified residuei | 433 | PhosphoserineBy similarity | 1 | |
Modified residuei | 443 | PhosphoserineBy similarity | 1 | |
Modified residuei | 521 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 521 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 590 | PhosphoserineCombined sources | 1 | |
Modified residuei | 593 | PhosphoserineCombined sources | 1 | |
Modified residuei | 626 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 630 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 635 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 655 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 655 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 655 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 662 | PhosphoserineBy similarity | 1 | |
Modified residuei | 664 | PhosphoserineBy similarity | 1 | |
Modified residuei | 669 | PhosphoserineBy similarity | 1 | |
Modified residuei | 671 | PhosphoserineBy similarity | 1 | |
Modified residuei | 760 | PhosphoserineBy similarity | 1 | |
Modified residuei | 761 | PhosphoserineBy similarity | 1 | |
Modified residuei | 765 | PhosphoserineBy similarity | 1 | |
Modified residuei | 767 | PhosphoserineBy similarity | 1 | |
Modified residuei | 794 | PhosphoserineBy similarity | 1 | |
Modified residuei | 853 | PhosphoserineBy similarity | 1 | |
Modified residuei | 860 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 869 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 922 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 976 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 1034 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1066 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 1082 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 1092 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 1191 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1216 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1242 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1249 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 1249 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 1301 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1303 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7) (PubMed:17873058). Serine pyrophosphorylation is achieved by Mg2+-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue (PubMed:17873058).1 Publication
Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O08784 |
jPOSTi | O08784 |
MaxQBi | O08784 |
PaxDbi | O08784 |
PeptideAtlasi | O08784 |
PRIDEi | O08784 |
PTM databases
iPTMneti | O08784 |
PhosphoSitePlusi | O08784 |
SwissPalmi | O08784 |
Expressioni
Tissue specificityi
Ubiquitous in adult and embryonic tissues.1 Publication
Developmental stagei
Expression elevated at 11 dpc when the branchial arches and facial swellings are present.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000024613, Expressed in thymus and 305 other tissues |
ExpressionAtlasi | O08784, baseline and differential |
Genevisiblei | O08784, MM |
Interactioni
Subunit structurei
Heterodimer; heterodimerizes with NOLC1 following monoubiquitination.
Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner.
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: MGI
- RNA polymerase I core binding Source: GO_Central
- scaffold protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204043, 6 interactors |
IntActi | O08784, 3 interactors |
MINTi | O08784 |
STRINGi | 10090.ENSMUSP00000135476 |
Miscellaneous databases
RNActi | O08784, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 38 | LisHPROSITE-ProRule annotationAdd BLAST | 33 | |
Repeati | 212 – 295 | 1Add BLAST | 84 | |
Repeati | 296 – 366 | 2Add BLAST | 71 | |
Repeati | 367 – 436 | 3Add BLAST | 70 | |
Repeati | 437 – 505 | 4Add BLAST | 69 | |
Repeati | 506 – 550 | 5Add BLAST | 45 | |
Repeati | 551 – 624 | 6Add BLAST | 74 | |
Repeati | 625 – 680 | 7Add BLAST | 56 | |
Repeati | 681 – 714 | 8Add BLAST | 34 | |
Repeati | 715 – 774 | 9Add BLAST | 60 | |
Repeati | 775 – 839 | 10Add BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 212 – 839 | 10 X approximate tandem repeatsAdd BLAST | 628 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 195 – 198 | Poly-Ser | 4 | |
Compositional biasi | 216 – 272 | Ala-richAdd BLAST | 57 | |
Compositional biasi | 253 – 272 | Poly-AlaAdd BLAST | 20 | |
Compositional biasi | 880 – 885 | Poly-Ser | 6 | |
Compositional biasi | 1179 – 1314 | Lys-richAdd BLAST | 136 | |
Compositional biasi | 1200 – 1205 | Poly-Lys | 6 | |
Compositional biasi | 1270 – 1277 | Poly-Lys | 8 | |
Compositional biasi | 1280 – 1288 | Poly-Lys | 9 | |
Compositional biasi | 1307 – 1314 | Poly-Lys | 8 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502S41N, Eukaryota |
GeneTreei | ENSGT00730000111382 |
HOGENOMi | CLU_263315_0_0_1 |
InParanoidi | O08784 |
PhylomeDBi | O08784 |
TreeFami | TF341730 |
Family and domain databases
InterProi | View protein in InterPro IPR006594, LisH IPR017859, Treacle IPR003993, Treacle_dom |
PANTHERi | PTHR20787, PTHR20787, 1 hit |
Pfami | View protein in Pfam PF03546, Treacle, 2 hits |
SMARTi | View protein in SMART SM00667, LisH, 1 hit |
PROSITEi | View protein in PROSITE PS50896, LISH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O08784-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY
60 70 80 90 100
THWQQTSELG QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER
110 120 130 140 150
AKATPRPTPV NSATAALPSK VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL
160 170 180 190 200
SGKSPKKSAE PLANTVLASE TEEEGNAQAL GPTAKSGTVS AGQGSSSSED
210 220 230 240 250
SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT KLGNVAPTPA
260 270 280 290 300
KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG
310 320 330 340 350
KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED
360 370 380 390 400
DSDSEDEMPV TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR
410 420 430 440 450
PVAAQAGKPE AKSSEESESD SGETPAAATL TTSPAKVKPL GKSSQVRPVS
460 470 480 490 500
TVTPGSSGKG ANLPCPGKVG SAALRVQMVK KEDVSESSSA ELDSDGPGSP
510 520 530 540 550
AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE EAAPAASAAQ
560 570 580 590 600
AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT
610 620 630 640 650
QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT
660 670 680 690 700
GPSVKAMAQE DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP
710 720 730 740 750
PASASGKAVA APTKGKPPVP NSTVSARGQR SVPAAGKAGA PATQAQKGPV
760 770 780 790 800
AGTGEDSESS SKEESDSEEE TPAQIKPVGK TSQVRAASAP AKESPKKGAH
810 820 830 840 850
PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA IKSPPVSVNR
860 870 880 890 900
NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST
910 920 930 940 950
YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL
960 970 980 990 1000
PMMPPQSAPI QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED
1010 1020 1030 1040 1050
EEDAKRPQMP KSAHRLDPDP SQKETVVEET PTESSEDEMV APSQSLLSGY
1060 1070 1080 1090 1100
MTPGLTVANS QASKATPRPD SNSLASSAPA TKDNPDGKQK SKSQHAADTA
1110 1120 1130 1140 1150
LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT QRLLEQAWPL
1160 1170 1180 1190 1200
SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK
1210 1220 1230 1240 1250
NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE
1260 1270 1280 1290 1300
KPDGELLGIK LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD
1310 1320
SASPIQKKKK KKKKSAEPAV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BL37 | H3BL37_MOUSE | Treacle protein | Tcof1 | 1,356 | Annotation score: | ||
H3BIX0 | H3BIX0_MOUSE | Treacle protein | Tcof1 | 1,056 | Annotation score: | ||
H3BK88 | H3BK88_MOUSE | Treacle protein | Tcof1 | 167 | Annotation score: | ||
F6R3V4 | F6R3V4_MOUSE | Treacle protein | Tcof1 | 1,013 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 | G → A in AAB60933 (PubMed:9158147).Curated | 1 | |
Sequence conflicti | 125 – 142 | Missing in AAB60933 (PubMed:9158147).CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001794 mRNA Translation: AAB71347.1 U81030 mRNA Translation: AAB60933.1 BC060105 mRNA Translation: AAH60105.1 |
CCDSi | CCDS37836.1 |
PIRi | JC5630 |
RefSeqi | NP_035682.1, NM_011552.3 |
Genome annotation databases
Ensembli | ENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613 |
GeneIDi | 21453 |
KEGGi | mmu:21453 |
UCSCi | uc008fbb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF001794 mRNA Translation: AAB71347.1 U81030 mRNA Translation: AAB60933.1 BC060105 mRNA Translation: AAH60105.1 |
CCDSi | CCDS37836.1 |
PIRi | JC5630 |
RefSeqi | NP_035682.1, NM_011552.3 |
3D structure databases
SMRi | O08784 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204043, 6 interactors |
IntActi | O08784, 3 interactors |
MINTi | O08784 |
STRINGi | 10090.ENSMUSP00000135476 |
PTM databases
iPTMneti | O08784 |
PhosphoSitePlusi | O08784 |
SwissPalmi | O08784 |
Proteomic databases
EPDi | O08784 |
jPOSTi | O08784 |
MaxQBi | O08784 |
PaxDbi | O08784 |
PeptideAtlasi | O08784 |
PRIDEi | O08784 |
Protocols and materials databases
Antibodypediai | 27956, 183 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613 |
GeneIDi | 21453 |
KEGGi | mmu:21453 |
UCSCi | uc008fbb.2, mouse |
Organism-specific databases
CTDi | 6949 |
MGIi | MGI:892003, Tcof1 |
Phylogenomic databases
eggNOGi | ENOG502S41N, Eukaryota |
GeneTreei | ENSGT00730000111382 |
HOGENOMi | CLU_263315_0_0_1 |
InParanoidi | O08784 |
PhylomeDBi | O08784 |
TreeFami | TF341730 |
Miscellaneous databases
BioGRID-ORCSi | 21453, 10 hits in 19 CRISPR screens |
ChiTaRSi | Tcof1, mouse |
PROi | PR:O08784 |
RNActi | O08784, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024613, Expressed in thymus and 305 other tissues |
ExpressionAtlasi | O08784, baseline and differential |
Genevisiblei | O08784, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006594, LisH IPR017859, Treacle IPR003993, Treacle_dom |
PANTHERi | PTHR20787, PTHR20787, 1 hit |
Pfami | View protein in Pfam PF03546, Treacle, 2 hits |
SMARTi | View protein in SMART SM00667, LisH, 1 hit |
PROSITEi | View protein in PROSITE PS50896, LISH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCOF_MOUSE | |
Accessioni | O08784Primary (citable) accession number: O08784 Secondary accession number(s): O08857 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot