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Protein

Treacle protein

Gene

Tcof1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Treacle protein
Alternative name(s):
Treacher Collins syndrome protein homolog
Gene namesi
Name:Tcof1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:892003 Tcof1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000724601 – 1320Treacle proteinAdd BLAST1320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1
Modified residuei104PhosphothreonineCombined sources1
Cross-linki126Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei132N6-acetyllysineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Modified residuei153N6-acetyllysineBy similarity1
Modified residuei154PhosphoserineCombined sources1
Modified residuei169PhosphoserineCombined sources1
Modified residuei171PhosphothreonineCombined sources1
Modified residuei301N6-acetyllysineBy similarity1
Modified residuei318N6-acetyllysineCombined sources1
Modified residuei324PhosphothreonineCombined sources1
Modified residuei327N6-acetyllysineCombined sources1
Modified residuei386PhosphoserineBy similarity1
Modified residuei413PhosphoserineCombined sources1
Modified residuei414PhosphoserineCombined sources1
Modified residuei433PhosphoserineBy similarity1
Modified residuei443PhosphoserineBy similarity1
Modified residuei521N6-acetyllysine; alternateBy similarity1
Cross-linki521Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei590PhosphoserineCombined sources1
Modified residuei593PhosphoserineCombined sources1
Modified residuei626N6-acetyllysineCombined sources1
Modified residuei630N6-acetyllysineCombined sources1
Cross-linki635Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei655N6-acetyllysine; alternateBy similarity1
Cross-linki655Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki655Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei662PhosphoserineBy similarity1
Modified residuei664PhosphoserineBy similarity1
Modified residuei669PhosphoserineBy similarity1
Modified residuei671PhosphoserineBy similarity1
Modified residuei760PhosphoserineBy similarity1
Modified residuei761PhosphoserineBy similarity1
Modified residuei765PhosphoserineBy similarity1
Modified residuei767PhosphoserineBy similarity1
Modified residuei794PhosphoserineBy similarity1
Modified residuei853PhosphoserineBy similarity1
Modified residuei860PhosphothreonineCombined sources1
Modified residuei869PhosphothreonineBy similarity1
Modified residuei922N6-acetyllysineCombined sources1
Modified residuei976N6-acetyllysineCombined sources1
Modified residuei1034PhosphoserineBy similarity1
Modified residuei1066PhosphothreonineCombined sources1
Cross-linki1082Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1092Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1191PhosphoserineCombined sources1
Modified residuei1216PhosphoserineCombined sources1
Modified residuei1242PhosphoserineBy similarity1
Modified residuei1249N6-acetyllysine; alternateBy similarity1
Cross-linki1249Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei1301PhosphoserineCombined sources1
Modified residuei1303PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated. Monoubiquitination by the BCR(KBTBD8) complex promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO08784
MaxQBiO08784
PaxDbiO08784
PeptideAtlasiO08784
PRIDEiO08784

PTM databases

iPTMnetiO08784
PhosphoSitePlusiO08784

Miscellaneous databases

PMAP-CutDBiO08784

Expressioni

Tissue specificityi

Ubiquitous in adult and embryonic tissues.1 Publication

Developmental stagei

Expression elevated at 11 dpc when the branchial arches and facial swellings are present.1 Publication

Gene expression databases

BgeeiENSMUSG00000024613 Expressed in 290 organ(s), highest expression level in thymus
ExpressionAtlasiO08784 baseline and differential
GenevisibleiO08784 MM

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with NOLC1 following monoubiquitination. Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with NOP56 in an RNA-independent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204043, 3 interactors
IntActiO08784, 3 interactors
MINTiO08784
STRINGi10090.ENSMUSP00000135639

Structurei

3D structure databases

ProteinModelPortaliO08784
SMRiO08784
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 38LisHPROSITE-ProRule annotationAdd BLAST33
Repeati212 – 2951Add BLAST84
Repeati296 – 3662Add BLAST71
Repeati367 – 4363Add BLAST70
Repeati437 – 5054Add BLAST69
Repeati506 – 5505Add BLAST45
Repeati551 – 6246Add BLAST74
Repeati625 – 6807Add BLAST56
Repeati681 – 7148Add BLAST34
Repeati715 – 7749Add BLAST60
Repeati775 – 83910Add BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 83910 X approximate tandem repeatsAdd BLAST628

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 198Poly-Ser4
Compositional biasi216 – 272Ala-richAdd BLAST57
Compositional biasi253 – 272Poly-AlaAdd BLAST20
Compositional biasi880 – 885Poly-Ser6
Compositional biasi1179 – 1314Lys-richAdd BLAST136
Compositional biasi1200 – 1205Poly-Lys6
Compositional biasi1270 – 1277Poly-Lys8
Compositional biasi1280 – 1288Poly-Lys9
Compositional biasi1307 – 1314Poly-Lys8

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF55 Eukaryota
ENOG411183W LUCA
GeneTreeiENSGT00730000111382
HOGENOMiHOG000154524
HOVERGENiHBG006664
InParanoidiO08784
KOiK14562
PhylomeDBiO08784
TreeFamiTF341730

Family and domain databases

InterProiView protein in InterPro
IPR006594 LisH
IPR017859 Treacle
IPR003993 Treacle_dom
PANTHERiPTHR20787 PTHR20787, 1 hit
PfamiView protein in Pfam
PF03546 Treacle, 2 hits
SMARTiView protein in SMART
SM00667 LisH, 1 hit
PROSITEiView protein in PROSITE
PS50896 LISH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O08784-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEARKRREL LPLIYHHLLQ AGYVRAAREV KEQSGQKSFL TQPVTLLDIY
60 70 80 90 100
THWQQTSELG QKQKAEDDET LQAKKSRVSD PVSSSESSDQ EKEEEAATER
110 120 130 140 150
AKATPRPTPV NSATAALPSK VKEKGKTKTA NKTVNSVSHP GSGKTVVHLL
160 170 180 190 200
SGKSPKKSAE PLANTVLASE TEEEGNAQAL GPTAKSGTVS AGQGSSSSED
210 220 230 240 250
SSISSDETDV EVKSPAKPAQ AKASAAPAKD PPARTAPGPT KLGNVAPTPA
260 270 280 290 300
KPARAAAAAA AAAVAAAAAA AAEESESSEE DSDSEDEAPA GLPSQVKASG
310 320 330 340 350
KGPHVRADSV SAKGISGKGP ILATPGKTGP AATQAKAERP EKDSETSSED
360 370 380 390 400
DSDSEDEMPV TVNTPQARTS GKSPRARGTS APAKESSQKG APAVTPGKAR
410 420 430 440 450
PVAAQAGKPE AKSSEESESD SGETPAAATL TTSPAKVKPL GKSSQVRPVS
460 470 480 490 500
TVTPGSSGKG ANLPCPGKVG SAALRVQMVK KEDVSESSSA ELDSDGPGSP
510 520 530 540 550
AKAKASLALP QKVRPVATQV KTDRGKGHSG SSEESSDSEE EAAPAASAAQ
560 570 580 590 600
AKPALEKQMK ASSRKGTPAS ATGASTSSHC KAGAVTSSAS LSSPALAKGT
610 620 630 640 650
QRSDVDSSSE SESEGAAPST PRVQGKSGGK GLQGKAALGQ GVAPVHTQKT
660 670 680 690 700
GPSVKAMAQE DSESLEEDSS SEEEDETPAQ ATPLGRLPQA KANPPPTKTP
710 720 730 740 750
PASASGKAVA APTKGKPPVP NSTVSARGQR SVPAAGKAGA PATQAQKGPV
760 770 780 790 800
AGTGEDSESS SKEESDSEEE TPAQIKPVGK TSQVRAASAP AKESPKKGAH
810 820 830 840 850
PGTPGKTGSS ATQAQPGKTE DSDSSSEESD SDTEMPSAQA IKSPPVSVNR
860 870 880 890 900
NSSPAVPAPT PEGVQAVNTT KKASGTTAQS SSSESEDGDE DLIPATQPST
910 920 930 940 950
YALRTSVTTP AALSRAASQP SKSEQSSRMP KGKKAKAAAS AQTSSAVETL
960 970 980 990 1000
PMMPPQSAPI QPKATNKLGK SKLPEKQQLA PGYPKAPRSS EDSSDTSSED
1010 1020 1030 1040 1050
EEDAKRPQMP KSAHRLDPDP SQKETVVEET PTESSEDEMV APSQSLLSGY
1060 1070 1080 1090 1100
MTPGLTVANS QASKATPRPD SNSLASSAPA TKDNPDGKQK SKSQHAADTA
1110 1120 1130 1140 1150
LPKTGRKEAS SGSTPQKPKK LKKSTSSSPA PTQTLPNSIT QRLLEQAWPL
1160 1170 1180 1190 1200
SEAQVQASVV KVLTELLEQE RLKATEAIKE SGKKSQKRKL SGDLEAGAPK
1210 1220 1230 1240 1250
NKKKKEQPVP RASAVSPEKA PMTSKAKSKL DKGSAGGKGK GSPGPQGAKE
1260 1270 1280 1290 1300
KPDGELLGIK LESGEQSDPK SKSKKKKSLK KKKDKEKKEK KKGKKSLAKD
1310 1320
SASPIQKKKK KKKKSAEPAV
Length:1,320
Mass (Da):135,001
Last modified:July 1, 1997 - v1
Checksum:i34D87F5F5D300758
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BL37H3BL37_MOUSE
Treacle protein
Tcof1
1,356Annotation score:
H3BIX0H3BIX0_MOUSE
Treacle protein
Tcof1
1,056Annotation score:
F6R3V4F6R3V4_MOUSE
Treacle protein
Tcof1
1,013Annotation score:
H3BK88H3BK88_MOUSE
Treacle protein
Tcof1
167Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35G → A in AAB60933 (PubMed:9158147).Curated1
Sequence conflicti125 – 142Missing in AAB60933 (PubMed:9158147).CuratedAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001794 mRNA Translation: AAB71347.1
U81030 mRNA Translation: AAB60933.1
BC060105 mRNA Translation: AAH60105.1
CCDSiCCDS37836.1
PIRiJC5630
RefSeqiNP_035682.1, NM_011552.3
UniGeneiMm.486251

Genome annotation databases

EnsembliENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613
GeneIDi21453
KEGGimmu:21453
UCSCiuc008fbb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001794 mRNA Translation: AAB71347.1
U81030 mRNA Translation: AAB60933.1
BC060105 mRNA Translation: AAH60105.1
CCDSiCCDS37836.1
PIRiJC5630
RefSeqiNP_035682.1, NM_011552.3
UniGeneiMm.486251

3D structure databases

ProteinModelPortaliO08784
SMRiO08784
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204043, 3 interactors
IntActiO08784, 3 interactors
MINTiO08784
STRINGi10090.ENSMUSP00000135639

PTM databases

iPTMnetiO08784
PhosphoSitePlusiO08784

Proteomic databases

EPDiO08784
MaxQBiO08784
PaxDbiO08784
PeptideAtlasiO08784
PRIDEiO08784

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000176630; ENSMUSP00000135476; ENSMUSG00000024613
GeneIDi21453
KEGGimmu:21453
UCSCiuc008fbb.2 mouse

Organism-specific databases

CTDi6949
MGIiMGI:892003 Tcof1

Phylogenomic databases

eggNOGiENOG410IF55 Eukaryota
ENOG411183W LUCA
GeneTreeiENSGT00730000111382
HOGENOMiHOG000154524
HOVERGENiHBG006664
InParanoidiO08784
KOiK14562
PhylomeDBiO08784
TreeFamiTF341730

Miscellaneous databases

PMAP-CutDBiO08784
PROiPR:O08784
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024613 Expressed in 290 organ(s), highest expression level in thymus
ExpressionAtlasiO08784 baseline and differential
GenevisibleiO08784 MM

Family and domain databases

InterProiView protein in InterPro
IPR006594 LisH
IPR017859 Treacle
IPR003993 Treacle_dom
PANTHERiPTHR20787 PTHR20787, 1 hit
PfamiView protein in Pfam
PF03546 Treacle, 2 hits
SMARTiView protein in SMART
SM00667 LisH, 1 hit
PROSITEiView protein in PROSITE
PS50896 LISH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTCOF_MOUSE
AccessioniPrimary (citable) accession number: O08784
Secondary accession number(s): O08857
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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