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Protein

AMP deaminase 3

Gene

Ampd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.

Catalytic activityi

AMP + H2O = IMP + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. AMP deaminase 1 (Ampd1), AMP deaminase 3 (Ampd3), AMP deaminase 2 (Ampd2)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi316Zinc; catalyticBy similarity1
Metal bindingi318Zinc; catalyticBy similarity1
Binding sitei318SubstrateBy similarity1
Metal bindingi585Zinc; catalyticBy similarity1
Binding sitei588SubstrateBy similarity1
Active sitei607Proton acceptorPROSITE-ProRule annotation1
Metal bindingi662Zinc; catalyticBy similarity1

GO - Molecular functioni

  • AMP deaminase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-74217 Purine salvage
UniPathwayi
UPA00591;UER00663

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase 3 (EC:3.5.4.6)
Alternative name(s):
AMP deaminase H-type
AMP deaminase isoform E
Heart-type AMPD
Gene namesi
Name:Ampd3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1096344 Ampd3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944111 – 766AMP deaminase 3Add BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineBy similarity1
Modified residuei107PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO08739
PaxDbiO08739
PeptideAtlasiO08739
PRIDEiO08739

PTM databases

iPTMnetiO08739
PhosphoSitePlusiO08739

Expressioni

Tissue specificityi

Found in heart, lung brain, spleen, kidney and to a lesser extent in liver.

Gene expression databases

BgeeiENSMUSG00000005686 Expressed in 260 organ(s), highest expression level in decidua
CleanExiMM_AMPD3
ExpressionAtlasiO08739 baseline and differential
GenevisibleiO08739 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiO08739, 3 interactors
MINTiO08739
STRINGi10090.ENSMUSP00000005829

Structurei

3D structure databases

ProteinModelPortaliO08739
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni387 – 392Substrate bindingBy similarity6
Regioni663 – 666Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1096 Eukaryota
COG1816 LUCA
GeneTreeiENSGT00390000008190
HOGENOMiHOG000092200
HOVERGENiHBG050494
InParanoidiO08739
KOiK01490
OMAiPSPDVWT
OrthoDBiEOG091G01OU
PhylomeDBiO08739
TreeFamiTF300439

Family and domain databases

CDDicd01319 AMPD, 1 hit
InterProiView protein in InterPro
IPR006650 A/AMP_deam_AS
IPR001365 A/AMP_deaminase_dom
IPR006329 AMPD
IPR029771 AMPD3
IPR032466 Metal_Hydrolase
PANTHERiPTHR11359 PTHR11359, 1 hit
PTHR11359:SF2 PTHR11359:SF2, 1 hit
PfamiView protein in Pfam
PF00962 A_deaminase, 1 hit
PIRSFiPIRSF001251 AMP_deaminase_met, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01429 AMP_deaminase, 1 hit
PROSITEiView protein in PROSITE
PS00485 A_DEAMINASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O08739-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPRQFPKLNM SDLDEHVRLL AEKVFAKVLR EEDSKDVMSL FTVPEDCPIG
60 70 80 90 100
QKEAKERELQ KELAEQKSVE TAKRKKSFKM IRSQSLSLQM PTQQDWKGPP
110 120 130 140 150
TASPAMSPAT PLVPGATSKP GPAPYAMPEY QRVTISGDYC AGITVEDYEQ
160 170 180 190 200
AAKSLAKALM IREKYARLAY HRFPRTTAQY LAHQGESVPL EEGLPDFHPP
210 220 230 240 250
PLPQEDPYCL DDAPPNLGYL VRMHGGVLFV YDNQTMLERQ EPHSLPYPDL
260 270 280 290 300
ETYIVDMSHI LALITDGPTK TYCHRRLNFL ESKFSLHEML NEMSEFKELK
310 320 330 340 350
SNPHRDFYNV RKVDTHIHAA ACMNQKHLLR FIKHTYQTEP DRTVAEKLGR
360 370 380 390 400
KITLRQVFDS LHMDPYDLTV DSLDVHAGRQ TFHRFDKFNS KYNPVGASEL
410 420 430 440 450
RDLYLKTENY LGGEYFARMV KEVARELEDS KYQYSEPRLS IYGRSPKEWS
460 470 480 490 500
SLARWFIQHK VYSPNMRWII QVPRIYDIFR SKKLLPNFGK MLENIFLPLF
510 520 530 540 550
KATINPQDHR ELHLFLKYVT GFDSVDDESK HSDHMFSDKS PSPDLWTSEQ
560 570 580 590 600
NPPYSYYLYY MYANIMVLNN LRRERGLSTF LFRPHCGEAG SITHLVSAFL
610 620 630 640 650
TADNISHGLL LKKSPVLQYL YYLAQIPIAM SPLSNNSLFL EYSKNPLREF
660 670 680 690 700
LHKGLHVSLS TDDPMQFHYT KEALMEEYAI AAQVWKLSTC DLCEIARNSV
710 720 730 740 750
LQSGLSHQEK QKFLGQNYYK EGPEGNDIRK TNVAQIRMAF RYETLCNELS
760
FLSDAMKSEE ITALTK
Length:766
Mass (Da):88,652
Last modified:July 27, 2011 - v2
Checksum:i94991133B18B8AA5
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SRX2A0A1L1SRX2_MOUSE
AMP deaminase
Ampd3
775Annotation score:
D3Z4N3D3Z4N3_MOUSE
AMP deaminase 3
Ampd3
184Annotation score:
D3YU73D3YU73_MOUSE
AMP deaminase 3
Ampd3
111Annotation score:
F6XQD0F6XQD0_MOUSE
AMP deaminase 3
Ampd3
137Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251E → K in BAA19933 (PubMed:9133604).Curated1
Sequence conflicti645N → K in BAA19933 (PubMed:9133604).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85596 mRNA Translation: BAA19933.1
D88994 Genomic DNA Translation: BAA32548.1 Sequence problems.
AK133465 mRNA Translation: BAE21671.1
CH466531 Genomic DNA Translation: EDL16988.1
CH466531 Genomic DNA Translation: EDL16989.1
CH466531 Genomic DNA Translation: EDL16990.1
BC040366 mRNA Translation: AAH40366.1
BC056380 mRNA Translation: AAH56380.1
CCDSiCCDS21747.1
RefSeqiNP_001263230.1, NM_001276301.1
NP_033797.2, NM_009667.3
XP_006507285.1, XM_006507222.3
UniGeneiMm.3238

Genome annotation databases

EnsembliENSMUST00000005829; ENSMUSP00000005829; ENSMUSG00000005686
ENSMUST00000170374; ENSMUSP00000130495; ENSMUSG00000005686
GeneIDi11717
KEGGimmu:11717
UCSCiuc009jfl.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85596 mRNA Translation: BAA19933.1
D88994 Genomic DNA Translation: BAA32548.1 Sequence problems.
AK133465 mRNA Translation: BAE21671.1
CH466531 Genomic DNA Translation: EDL16988.1
CH466531 Genomic DNA Translation: EDL16989.1
CH466531 Genomic DNA Translation: EDL16990.1
BC040366 mRNA Translation: AAH40366.1
BC056380 mRNA Translation: AAH56380.1
CCDSiCCDS21747.1
RefSeqiNP_001263230.1, NM_001276301.1
NP_033797.2, NM_009667.3
XP_006507285.1, XM_006507222.3
UniGeneiMm.3238

3D structure databases

ProteinModelPortaliO08739
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08739, 3 interactors
MINTiO08739
STRINGi10090.ENSMUSP00000005829

PTM databases

iPTMnetiO08739
PhosphoSitePlusiO08739

Proteomic databases

MaxQBiO08739
PaxDbiO08739
PeptideAtlasiO08739
PRIDEiO08739

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005829; ENSMUSP00000005829; ENSMUSG00000005686
ENSMUST00000170374; ENSMUSP00000130495; ENSMUSG00000005686
GeneIDi11717
KEGGimmu:11717
UCSCiuc009jfl.2 mouse

Organism-specific databases

CTDi272
MGIiMGI:1096344 Ampd3

Phylogenomic databases

eggNOGiKOG1096 Eukaryota
COG1816 LUCA
GeneTreeiENSGT00390000008190
HOGENOMiHOG000092200
HOVERGENiHBG050494
InParanoidiO08739
KOiK01490
OMAiPSPDVWT
OrthoDBiEOG091G01OU
PhylomeDBiO08739
TreeFamiTF300439

Enzyme and pathway databases

UniPathwayi
UPA00591;UER00663

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-74217 Purine salvage

Miscellaneous databases

PROiPR:O08739
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005686 Expressed in 260 organ(s), highest expression level in decidua
CleanExiMM_AMPD3
ExpressionAtlasiO08739 baseline and differential
GenevisibleiO08739 MM

Family and domain databases

CDDicd01319 AMPD, 1 hit
InterProiView protein in InterPro
IPR006650 A/AMP_deam_AS
IPR001365 A/AMP_deaminase_dom
IPR006329 AMPD
IPR029771 AMPD3
IPR032466 Metal_Hydrolase
PANTHERiPTHR11359 PTHR11359, 1 hit
PTHR11359:SF2 PTHR11359:SF2, 1 hit
PfamiView protein in Pfam
PF00962 A_deaminase, 1 hit
PIRSFiPIRSF001251 AMP_deaminase_met, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01429 AMP_deaminase, 1 hit
PROSITEiView protein in PROSITE
PS00485 A_DEAMINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMPD3_MOUSE
AccessioniPrimary (citable) accession number: O08739
Secondary accession number(s): O88692, Q8CFR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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