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Protein

Bcl-2 homologous antagonist/killer

Gene

Bak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi158Zinc; shared with dimeric partnerBy similarity1
Metal bindingi162Zinc; shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Host-virus interaction
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-111452 Activation and oligomerization of BAK protein
R-MMU-111457 Release of apoptotic factors from the mitochondria

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2 homologous antagonist/killer
Alternative name(s):
Apoptosis regulator BAK
Gene namesi
Name:Bak1
Synonyms:Bak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1097161 Bak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei186 – 203HelicalSequence analysisAdd BLAST18

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001430602 – 209Bcl-2 homologous antagonist/killerAdd BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO08734
MaxQBiO08734
PaxDbiO08734
PeptideAtlasiO08734
PRIDEiO08734
TopDownProteomicsiO08734

PTM databases

iPTMnetiO08734
PhosphoSitePlusiO08734
SwissPalmiO08734

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000057789 Expressed in 219 organ(s), highest expression level in ileum
CleanExiMM_BAK1
ExpressionAtlasiO08734 baseline and differential
GenevisibleiO08734 MM

Interactioni

Subunit structurei

Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2 and BCL2L1 isoform Bcl-X(L). Forms heterooligomers with BAX (By similarity). Interacts with BCL2A1 (PubMed:18462686). Interacts withRTL10/BOP (PubMed:18462686). Interacts with VDAC1 (By similarity).By similarity1 Publication
(Microbial infection) Interacts with gamma-herpesvirus 68 protein vBCL2.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-2033 BAK1 oligomer
CORUMiO08734
DIPiDIP-29807N
IntActiO08734, 10 interactors
MINTiO08734
STRINGi10090.ENSMUSP00000077757

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO08734
SMRiO08734
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08734

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi72 – 86BH3Add BLAST15
Motifi115 – 134BH1Add BLAST20
Motifi167 – 182BH2Add BLAST16

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4728 Eukaryota
ENOG41123S0 LUCA
GeneTreeiENSGT00530000062935
HOGENOMiHOG000006521
HOVERGENiHBG002674
InParanoidiO08734
KOiK14021
OMAiINWGRVI
OrthoDBiEOG091G0OCU
TreeFamiTF315834

Family and domain databases

Gene3Di1.10.437.10, 1 hit
InterProiView protein in InterPro
IPR026308 BAK
IPR002475 Bcl2-like
IPR020717 Bcl2_BH1_motif_CS
IPR020726 Bcl2_BH2_motif_CS
IPR020728 Bcl2_BH3_motif_CS
IPR036834 Blc2-like_sf
IPR026298 Blc2_fam
PANTHERiPTHR11256 PTHR11256, 1 hit
PTHR11256:SF41 PTHR11256:SF41, 1 hit
PfamiView protein in Pfam
PF00452 Bcl-2, 1 hit
PRINTSiPR01862 BCL2FAMILY
SUPFAMiSSF56854 SSF56854, 1 hit
PROSITEiView protein in PROSITE
PS50062 BCL2_FAMILY, 1 hit
PS01080 BH1, 1 hit
PS01258 BH2, 1 hit
PS01259 BH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O08734-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGQGPGPP KVGCDESPSP SEQQVAQDTE EVFRSYVFYL HQQEQETQGA
60 70 80 90 100
AAPANPEMDN LPLEPNSILG QVGRQLALIG DDINRRYDTE FQNLLEQLQP
110 120 130 140 150
TAGNAYELFT KIASSLFKSG ISWGRVVALL GFGYRLALYV YQRGLTGFLG
160 170 180 190 200
QVTCFLADII LHHYIARWIA QRGGWVAALN FRRDPILTVM VIFGVVLLGQ

FVVHRFFRS
Length:209
Mass (Da):23,295
Last modified:April 1, 2015 - v3
Checksum:i49972D3CA21F4218
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7CUQ1F7CUQ1_MOUSE
Bcl-2 homologous antagonist/killer
Bak1
95Annotation score:
Q91WX5Q91WX5_MOUSE
Bak1 protein
Bak1
151Annotation score:

Sequence cautioni

The sequence CAA73684 differs from that shown. Reason: Frameshift at position 50.

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50 – 52AAA → RP in CAA73684 (PubMed:9299236).Curated3
Sequence conflicti134Y → H in BAC40796 (PubMed:16141072).Curated1
Sequence conflicti181F → L in CAA73684 (PubMed:9299236).Curated1
Sequence conflicti181F → L in BAC40796 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13231 mRNA Translation: CAA73684.1 Frameshift.
AK089220 mRNA Translation: BAC40796.1
AC132404 Genomic DNA No translation available.
CCDSiCCDS28558.1
RefSeqiNP_031549.2, NM_007523.2
UniGeneiMm.2443

Genome annotation databases

EnsembliENSMUST00000078691; ENSMUSP00000077757; ENSMUSG00000057789
GeneIDi12018
KEGGimmu:12018
UCSCiuc008bff.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13231 mRNA Translation: CAA73684.1 Frameshift.
AK089220 mRNA Translation: BAC40796.1
AC132404 Genomic DNA No translation available.
CCDSiCCDS28558.1
RefSeqiNP_031549.2, NM_007523.2
UniGeneiMm.2443

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VOHX-ray1.90B65-90[»]
5FMJX-ray2.43B61-94[»]
5KTGX-ray2.80A/B66-144[»]
ProteinModelPortaliO08734
SMRiO08734
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-2033 BAK1 oligomer
CORUMiO08734
DIPiDIP-29807N
IntActiO08734, 10 interactors
MINTiO08734
STRINGi10090.ENSMUSP00000077757

PTM databases

iPTMnetiO08734
PhosphoSitePlusiO08734
SwissPalmiO08734

Proteomic databases

EPDiO08734
MaxQBiO08734
PaxDbiO08734
PeptideAtlasiO08734
PRIDEiO08734
TopDownProteomicsiO08734

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078691; ENSMUSP00000077757; ENSMUSG00000057789
GeneIDi12018
KEGGimmu:12018
UCSCiuc008bff.1 mouse

Organism-specific databases

CTDi578
MGIiMGI:1097161 Bak1

Phylogenomic databases

eggNOGiKOG4728 Eukaryota
ENOG41123S0 LUCA
GeneTreeiENSGT00530000062935
HOGENOMiHOG000006521
HOVERGENiHBG002674
InParanoidiO08734
KOiK14021
OMAiINWGRVI
OrthoDBiEOG091G0OCU
TreeFamiTF315834

Enzyme and pathway databases

ReactomeiR-MMU-111452 Activation and oligomerization of BAK protein
R-MMU-111457 Release of apoptotic factors from the mitochondria

Miscellaneous databases

EvolutionaryTraceiO08734
PROiPR:O08734
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057789 Expressed in 219 organ(s), highest expression level in ileum
CleanExiMM_BAK1
ExpressionAtlasiO08734 baseline and differential
GenevisibleiO08734 MM

Family and domain databases

Gene3Di1.10.437.10, 1 hit
InterProiView protein in InterPro
IPR026308 BAK
IPR002475 Bcl2-like
IPR020717 Bcl2_BH1_motif_CS
IPR020726 Bcl2_BH2_motif_CS
IPR020728 Bcl2_BH3_motif_CS
IPR036834 Blc2-like_sf
IPR026298 Blc2_fam
PANTHERiPTHR11256 PTHR11256, 1 hit
PTHR11256:SF41 PTHR11256:SF41, 1 hit
PfamiView protein in Pfam
PF00452 Bcl-2, 1 hit
PRINTSiPR01862 BCL2FAMILY
SUPFAMiSSF56854 SSF56854, 1 hit
PROSITEiView protein in PROSITE
PS50062 BCL2_FAMILY, 1 hit
PS01080 BH1, 1 hit
PS01258 BH2, 1 hit
PS01259 BH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBAK_MOUSE
AccessioniPrimary (citable) accession number: O08734
Secondary accession number(s): E9QM21, Q8C264
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 1, 2015
Last modified: November 7, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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