UniProtKB - O08730 (GLYG_RAT)
Glycogenin-1
Gyg1
Functioni
Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.
By similarityCatalytic activityi
- L-tyrosyl-[glycogenin] + UDP-α-D-glucose = α-D-glucosyl-L-tyrosyl-[glycogenin] + H+ + UDPBy similarityEC:2.4.1.186By similarity
- [1,4-α-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-α-D-glucose = [1,4-α-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + H+ + UDPBy similarityEC:2.4.1.186By similarity
Cofactori
: glycogen biosynthesis Pathwayi
This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateBy similarity | 1 | |
Sitei | 86 | Important for catalytic activityBy similarity | 1 | |
Metal bindingi | 102 | ManganeseBy similarity | 1 | |
Metal bindingi | 104 | ManganeseBy similarity | 1 | |
Metal bindingi | 212 | ManganeseBy similarity | 1 |
GO - Molecular functioni
- glucose binding Source: RGD
- glycogenin glucosyltransferase activity Source: RGD
- glycosyltransferase activity Source: GO_Central
- manganese ion binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- UDP-alpha-D-glucose:glucosyl-glycogenin alpha-D-glucosyltransferase activity Source: UniProtKB
GO - Biological processi
- glycogen biosynthetic process Source: RGD
Keywordsi
Molecular function | Transferase |
Biological process | Glycogen biosynthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-3322077, Glycogen synthesis R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
UniPathwayi | UPA00164 |
Protein family/group databases
CAZyi | GT8, Glycosyltransferase Family 8 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Gyg1 Synonyms:Gyg |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621785, Gyg1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000215179 | 2 – 333 | Glycogenin-1CuratedAdd BLAST | 332 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineBy similarity | 1 | |
Modified residuei | 44 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 195 | O-linked (Glc...) tyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | O08730 |
PaxDbi | O08730 |
PTM databases
GlyGeni | O08730, 1 site |
iPTMneti | O08730 |
PhosphoSitePlusi | O08730 |
Interactioni
Subunit structurei
Homodimer.
Interacts (via C-terminus) with glycogen synthase GYS1 (By similarity).
Interacts (via C-terminus) with glycogen synthase GYS2 (By similarity). This interaction is required for GYS2-mediated glycogen synthesis (By similarity).
By similarityGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
IntActi | O08730, 1 interactor |
STRINGi | 10116.ENSRNOP00000014837 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 15 | Substrate bindingBy similarity | 7 | |
Regioni | 102 – 104 | Substrate bindingBy similarity | 3 | |
Regioni | 133 – 135 | Substrate bindingBy similarity | 3 | |
Regioni | 160 – 164 | Substrate bindingBy similarity | 5 | |
Regioni | 212 – 218 | Substrate bindingBy similarity | 7 | |
Regioni | 284 – 316 | Interaction with GYS1By similarityAdd BLAST | 33 | |
Regioni | 287 – 316 | DisorderedSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1950, Eukaryota |
InParanoidi | O08730 |
OrthoDBi | 1424146at2759 |
PhylomeDBi | O08730 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002495, Glyco_trans_8 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01501, Glyco_transf_8, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTDQAFVTLT TNDAYAKGAL VLGSSLKQHR TTRRTVVLAS PQVSDSMRKV
60 70 80 90 100
LETVFDEVIM VDVLDSGDSA HLTLMKRPEL GITLTKLHCW SLTQYSKCVF
110 120 130 140 150
MDADTLVLSN IDDLFEREEL SAAPDPGWPD CFNSGVFVYQ PSIETYNQLL
160 170 180 190 200
HLASEQGSFD GGDQGLLNTY FSGWATTDIT KHLPFVYNLS SLSIYSYLPA
210 220 230 240 250
FKAFGKNAKV VHFLGRTKPW NYTYNPQTKS VKCESQDPIV SHPEFLNLWW
260 270 280 290 300
DTFTTNVLPL LQHHGLVKDA GSYLMMEHVT GALSDLSFGE APPASQPSLS
310 320 330
SEERKERWEQ GQADYMGADS FDNIKRKLDT YLQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WFR6 | F8WFR6_RAT | Glycogenin-1 | Gyg1 | 331 | Annotation score: | ||
A0A0G2JXP1 | A0A0G2JXP1_RAT | Glycogenin-1 | Gyg1 | 361 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF021343 mRNA Translation: AAB81219.1 U96130 mRNA Translation: AAB53334.1 BC070944 mRNA Translation: AAH70944.1 |
RefSeqi | NP_112305.1, NM_031043.2 |
Genome annotation databases
GeneIDi | 81675 |
KEGGi | rno:81675 |
UCSCi | RGD:621785, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF021343 mRNA Translation: AAB81219.1 U96130 mRNA Translation: AAB53334.1 BC070944 mRNA Translation: AAH70944.1 |
RefSeqi | NP_112305.1, NM_031043.2 |
3D structure databases
SMRi | O08730 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08730, 1 interactor |
STRINGi | 10116.ENSRNOP00000014837 |
Protein family/group databases
CAZyi | GT8, Glycosyltransferase Family 8 |
PTM databases
GlyGeni | O08730, 1 site |
iPTMneti | O08730 |
PhosphoSitePlusi | O08730 |
Proteomic databases
jPOSTi | O08730 |
PaxDbi | O08730 |
Genome annotation databases
GeneIDi | 81675 |
KEGGi | rno:81675 |
UCSCi | RGD:621785, rat |
Organism-specific databases
CTDi | 2992 |
RGDi | 621785, Gyg1 |
Phylogenomic databases
eggNOGi | KOG1950, Eukaryota |
InParanoidi | O08730 |
OrthoDBi | 1424146at2759 |
PhylomeDBi | O08730 |
Enzyme and pathway databases
UniPathwayi | UPA00164 |
Reactomei | R-RNO-3322077, Glycogen synthesis R-RNO-6798695, Neutrophil degranulation R-RNO-70221, Glycogen breakdown (glycogenolysis) |
Miscellaneous databases
PROi | PR:O08730 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002495, Glyco_trans_8 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01501, Glyco_transf_8, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLYG_RAT | |
Accessioni | O08730Primary (citable) accession number: O08730 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families