UniProtKB - O08696 (FOXM1_MOUSE)
Protein
Forkhead box protein M1
Gene
Foxm1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 233 – 325 | Fork-headPROSITE-ProRule annotationAdd BLAST | 93 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription factor activity Source: UniProtKB
- protein kinase binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator Source: UniProtKB
- DNA repair Source: UniProtKB-KW
- G2/M transition of mitotic cell cycle Source: UniProtKB
- liver development Source: MGI
- negative regulation of cell aging Source: MGI
- negative regulation of stress-activated MAPK cascade Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of double-strand break repair Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of cell cycle arrest Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of Ras protein signal transduction Source: MGI
- regulation of reactive oxygen species metabolic process Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- vasculogenesis Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Cell cycle, DNA damage, DNA repair, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-156711, Polo-like kinase mediated events R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition |
Names & Taxonomyi
Protein namesi | Recommended name: Forkhead box protein M1Alternative name(s): Forkhead homolog 16 Winged-helix transcription factor Trident |
Gene namesi | Name:Foxm1 Synonyms:Fkh16 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347487, Foxm1 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091864 | 1 – 760 | Forkhead box protein M1Add BLAST | 760 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 199 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 323 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 329 | PhosphoserineBy similarity | 1 | |
Cross-linki | 354 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 374 | Phosphoserine; by CHEK2By similarity | 1 | |
Cross-linki | 420 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 438 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 521 | PhosphoserineBy similarity | 1 | |
Modified residuei | 608 | Phosphothreonine; by CDK1By similarity | 1 | |
Modified residuei | 624 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 727 | Phosphoserine; by PLK1By similarity | 1 | |
Modified residuei | 736 | Phosphoserine; by PLK1By similarity | 1 |
Post-translational modificationi
Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | O08696 |
PaxDbi | O08696 |
PRIDEi | O08696 |
ProteomicsDBi | 271797 |
PTM databases
iPTMneti | O08696 |
PhosphoSitePlusi | O08696 |
Expressioni
Tissue specificityi
Expressed in fetal heart, brain, liver, lung, kidney and limb, but only in adult thymus. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors.
Developmental stagei
Expressed at 14 dpc in the embryo.
Interactioni
GO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
IntActi | O08696, 2 interactors |
MINTi | O08696 |
STRINGi | 10090.ENSMUSP00000073041 |
Miscellaneous databases
RNActi | O08696, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 479 – 512 | Glu/Pro/Ser/Thr-richAdd BLAST | 34 |
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
InParanoidi | O08696 |
PhylomeDBi | O08696 |
Family and domain databases
CDDi | cd00059, FH, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001766, Fork_head_dom IPR042839, FOXM1 IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR46878, PTHR46878, 1 hit |
Pfami | View protein in Pfam PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O08696-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTSPRRPLI LKRRRLPLPV QNAPSETSEE EAKRSPAQPE PAPAQASQEV
60 70 80 90 100
AESSSCKFPA GIKIINHPTT PNTQVVAIPS NADIQSIITA LTAKGKESGT
110 120 130 140 150
SGPNRFILIS SGGPSSHPSQ PQAHSSRDSK RAEVITETLG PKPAAKGVPV
160 170 180 190 200
PKPPGAPPRQ RQESYAGGEA AGCTLDNSLT NIQWLGKMSS DGLGPCSVKQ
210 220 230 240 250
ELEEKENCHL EQNRVKVEEP SGVSTSWQDS VSERPPYSYM AMIQFAINST
260 270 280 290 300
ERKRMTLKDI YTWIEDHFPY FKHIAKPGWK NSIRHNLSLH DMFVRETSAN
310 320 330 340 350
GKVSFWTIHP SANRHLTLDQ VFKPLEPGSP QSPEHLESQQ KRPNPELHRN
360 370 380 390 400
VTIKTEIPLG ARRKMKPLLP RVSSYLEPIQ FPVNQSLVLQ PSVKVPFRLA
410 420 430 440 450
ASLMSSELAR HSKRVRIAPK VLLSSEGIAP LPATEPPKEE KPLLGGEGLL
460 470 480 490 500
PLLPIQSIKE EEMQPEEDIA HLERPIKVES PPLEEWPSPC ASLKEELSNS
510 520 530 540 550
WEDSSCSPTP KPKKSYCGLK SPTRCVSEML VTKRREKREV SRSRRKQHLQ
560 570 580 590 600
PPCLDEPDLF FPEDSSTFRP AVELLAESSE PAPHLSCPQE EGGPFKTPIK
610 620 630 640 650
ETLPVSSTPS KSVLSRDPES WRLTPPAKVG GLDFSPVRTP QGAFGLLPDS
660 670 680 690 700
LGLMELNTTP LKSGPLFDSP RELLNSEPFD LASDPFGSPP PPHVEGPKPG
710 720 730 740 750
SPELQIPSLS ANRSLTEGLV LDTMNDSLSK ILLDISFPGL EEDPLGPDNI
760
NWSQFIPELR
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6P1H7 | Q6P1H7_MOUSE | Forkhead box M1 | Foxm1 | 757 | Annotation score: | ||
E9Q7Y0 | E9Q7Y0_MOUSE | Forkhead box protein M1 | Foxm1 | 742 | Annotation score: | ||
A0A0N4SVY9 | A0A0N4SVY9_MOUSE | Forkhead box protein M1 | Foxm1 | 402 | Annotation score: | ||
A0A0N4SWD2 | A0A0N4SWD2_MOUSE | Forkhead box protein M1 | Foxm1 | 214 | Annotation score: |
Sequence cautioni
The sequence CAA72115 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11245 mRNA Translation: CAA72115.1 Sequence problems. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y11245 mRNA Translation: CAA72115.1 Sequence problems. |
3D structure databases
SMRi | O08696 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08696, 2 interactors |
MINTi | O08696 |
STRINGi | 10090.ENSMUSP00000073041 |
PTM databases
iPTMneti | O08696 |
PhosphoSitePlusi | O08696 |
Proteomic databases
MaxQBi | O08696 |
PaxDbi | O08696 |
PRIDEi | O08696 |
ProteomicsDBi | 271797 |
Organism-specific databases
MGIi | MGI:1347487, Foxm1 |
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
InParanoidi | O08696 |
PhylomeDBi | O08696 |
Enzyme and pathway databases
Reactomei | R-MMU-156711, Polo-like kinase mediated events R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition |
Miscellaneous databases
ChiTaRSi | Foxm1, mouse |
PROi | PR:O08696 |
RNActi | O08696, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00059, FH, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR001766, Fork_head_dom IPR042839, FOXM1 IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR46878, PTHR46878, 1 hit |
Pfami | View protein in Pfam PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FOXM1_MOUSE | |
Accessioni | O08696Primary (citable) accession number: O08696 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot