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Protein

Arginase-2, mitochondrial

Gene

Arg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4+ and CD8+ T cells (PubMed:27745970, PubMed:25009204). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (PubMed:27074721). May play a role in promoting prenatal immune suppression (By similarity). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (By similarity).By similarity6 Publications

Catalytic activityi

L-arginine + H2O = L-ornithine + urea.By similarity

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Arginase-2, mitochondrial (Arg2), Arginase-1 (Arg1), Arginase (Arg2)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine and urea from L-arginine, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi120Manganese 1PROSITE-ProRule annotation1
Metal bindingi143Manganese 1PROSITE-ProRule annotation1
Metal bindingi143Manganese 2PROSITE-ProRule annotation1
Metal bindingi145Manganese 2PROSITE-ProRule annotation1
Metal bindingi147Manganese 1PROSITE-ProRule annotation1
Binding sitei202SubstrateBy similarity1
Metal bindingi251Manganese 1PROSITE-ProRule annotation1
Metal bindingi251Manganese 2PROSITE-ProRule annotation1
Metal bindingi253Manganese 2PROSITE-ProRule annotation1
Binding sitei265SubstrateBy similarity1
Binding sitei296SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • arginine catabolic process to ornithine Source: MGI
  • arginine metabolic process Source: MGI
  • innate immune response Source: UniProtKB-KW
  • negative regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: UniProtKB
  • negative regulation of CD4-positive, alpha-beta T cell proliferation Source: UniProtKB
  • negative regulation of chemokine (C-C motif) ligand 4 production Source: UniProtKB
  • negative regulation of chemokine (C-C motif) ligand 5 production Source: UniProtKB
  • negative regulation of defense response to bacterium Source: UniProtKB
  • negative regulation of interleukin-13 secretion Source: UniProtKB
  • negative regulation of interleukin-17 secretion Source: UniProtKB
  • negative regulation of macrophage inflammatory protein 1 alpha production Source: UniProtKB
  • negative regulation of nitric-oxide synthase activity Source: MGI
  • negative regulation of striated muscle contraction Source: MGI
  • negative regulation of tumor necrosis factor production Source: MGI
  • negative regulation of tumor necrosis factor secretion Source: UniProtKB
  • negative regulation of type 2 immune response Source: UniProtKB
  • positive regulation of cellular senescence Source: MGI
  • regulation of interleukin-1 beta secretion Source: UniProtKB
  • regulation of L-arginine import Source: MGI
  • regulation of reactive oxygen species biosynthetic process Source: UniProtKB
  • striated muscle contraction Source: MGI
  • urea cycle Source: UniProtKB-UniPathway
  • ureteric bud development Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processAdaptive immunity, Arginine metabolism, Immunity, Innate immunity, Urea cycle
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-70635 Urea cycle
UniPathwayiUPA00158; UER00270

Names & Taxonomyi

Protein namesi
Recommended name:
Arginase-2, mitochondrial (EC:3.5.3.1By similarity)
Alternative name(s):
Arginase II
Kidney-type arginase
Non-hepatic arginase
Type II arginase
Gene namesi
Name:Arg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1330806 Arg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi160H → F: Catalytically inactive; defective in promoting NOS3/eNOS-uncoupling in endothelial cells. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000000208523 – 354Arginase-2, mitochondrialAdd BLAST332

Proteomic databases

MaxQBiO08691
PaxDbiO08691
PeptideAtlasiO08691
PRIDEiO08691

2D gel databases

REPRODUCTION-2DPAGEiO08691

PTM databases

iPTMnetiO08691
PhosphoSitePlusiO08691

Expressioni

Gene expression databases

BgeeiENSMUSG00000021125
CleanExiMM_ARG2
GenevisibleiO08691 MM

Interactioni

Subunit structurei

Homotrimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198191, 1 interactor
IntActiO08691, 1 interactor
MINTiO08691
STRINGi10090.ENSMUSP00000021550

Structurei

3D structure databases

ProteinModelPortaliO08691
SMRiO08691
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 149Substrate bindingBy similarity5
Regioni156 – 158Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2965 Eukaryota
COG0010 LUCA
GeneTreeiENSGT00530000063082
HOGENOMiHOG000204319
HOVERGENiHBG003030
InParanoidiO08691
KOiK01476
OMAiSAVARHC
OrthoDBiEOG091G0A38
PhylomeDBiO08691
TreeFamiTF300034

Family and domain databases

InterProiView protein in InterPro
IPR014033 Arginase
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
IPR020855 Ureohydrolase_Mn_BS
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PIRSFiPIRSF036979 Arginase, 1 hit
PRINTSiPR00116 ARGINASE
SUPFAMiSSF52768 SSF52768, 1 hit
TIGRFAMsiTIGR01229 rocF_arginase, 1 hit
PROSITEiView protein in PROSITE
PS01053 ARGINASE_1, 1 hit
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLRSSASRL LHGQIPCVLT RSVHSVAIVG APFSRGQKKL GVEYGPAAIR
60 70 80 90 100
EAGLLKRLSR LGCHLKDFGD LSFTNVPQDD PYNNLVVYPR SVGLANQELA
110 120 130 140 150
EVVSRAVSGG YSCVTMGGDH SLAIGTIIGH ARHRPDLCVI WVDAHADINT
160 170 180 190 200
PLTTVSGNIH GQPLSFLIKE LQDKVPQLPG FSWIKPCLSP PNIVYIGLRD
210 220 230 240 250
VEPPEHFILK NYDIQYFSMR EIDRLGIQKV MEQTFDRLIG KRQRPIHLSF
260 270 280 290 300
DIDAFDPKLA PATGTPVVGG LTYREGVYIT EEIHNTGLLS ALDLVEVNPH
310 320 330 340 350
LATSEEEAKA TARLAVDVIA SSFGQTREGG HIVYDHLPTP SSPHESENEE

CVRI
Length:354
Mass (Da):38,878
Last modified:July 1, 1997 - v1
Checksum:iB372DF68A19473F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90886 mRNA Translation: AAC22548.1
AF032466 mRNA Translation: AAB86959.1
AF045965
, AF044680, AF045959, AF045960, AF045961, AF045962, AF045963, AF045964 Genomic DNA Translation: AAC78460.1
CCDSiCCDS26007.1
RefSeqiNP_033835.1, NM_009705.3
UniGeneiMm.3506

Genome annotation databases

EnsembliENSMUST00000021550; ENSMUSP00000021550; ENSMUSG00000021125
GeneIDi11847
KEGGimmu:11847
UCSCiuc007nzv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiARGI2_MOUSE
AccessioniPrimary (citable) accession number: O08691
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 20, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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