Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Semaphorin-3A

Gene

Sema3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).By similarity

GO - Molecular functioni

  • chemorepellent activity Source: MGI
  • neuropilin binding Source: BHF-UCL
  • semaphorin receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-399954 Sema3A PAK dependent Axon repulsion
R-MMU-399955 SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
R-MMU-399956 CRMPs in Sema3A signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-3A
Alternative name(s):
Semaphorin III
Short name:
Sema III
Semaphorin-D
Short name:
Sema D
Gene namesi
Name:Sema3a
Synonyms:Semad, SemD
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107558 Sema3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003230421 – 772Semaphorin-3AAdd BLAST752

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi103 ↔ 1141 Publication
Glycosylationi125N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi132 ↔ 1411 Publication
Disulfide bondi269 ↔ 3811 Publication
Disulfide bondi293 ↔ 3411 Publication
Disulfide bondi517 ↔ 535By similarity
Glycosylationi591N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi650 ↔ 723By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08665
PaxDbiO08665
PeptideAtlasiO08665
PRIDEiO08665

PTM databases

iPTMnetiO08665
PhosphoSitePlusiO08665

Expressioni

Developmental stagei

Expressed early in embryonic development (E11) in distinct regions of the neuroectoderm and mesoderm. Expression became more extensive at later stages.

Gene expression databases

BgeeiENSMUSG00000028883 Expressed in 279 organ(s), highest expression level in retina
ExpressionAtlasiO08665 baseline and differential
GenevisibleiO08665 MM

Interactioni

Subunit structurei

Interacts with PXND1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Nrp1P973333EBI-8586029,EBI-1555129

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203161, 2 interactors
DIPiDIP-59997N
IntActiO08665, 2 interactors
MINTiO08665
STRINGi10090.ENSMUSP00000030714

Structurei

Secondary structure

1772
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO08665
SMRiO08665
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08665

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 514SemaPROSITE-ProRule annotationAdd BLAST484
Domaini579 – 665Ig-like C2-typeAdd BLAST87

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi728 – 770Arg/Lys-rich (basic)Add BLAST43

Domaini

Strong binding to neuropilin is mediated by the carboxy third of the protein.

Sequence similaritiesi

Belongs to the semaphorin family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiKOG3611 Eukaryota
ENOG410XQZC LUCA
GeneTreeiENSGT00760000118854
HOGENOMiHOG000039964
HOVERGENiHBG055071
InParanoidiO08665
KOiK06840
OMAiREPTTIS
OrthoDBiEOG091G01W0
TreeFamiTF316102

Family and domain databases

Gene3Di2.130.10.10, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR016201 PSI
IPR001627 Semap_dom
IPR036352 Semap_dom_sf
IPR027231 Semaphorin
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR11036 PTHR11036, 1 hit
PfamiView protein in Pfam
PF01403 Sema, 1 hit
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00423 PSI, 1 hit
SM00630 Sema, 1 hit
SUPFAMiSSF101912 SSF101912, 1 hit
SSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51004 SEMA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O08665-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGWFTGIACL FWGVLLTARA NYANGKNNVP RLKLSYKEML ESNNVITFNG
60 70 80 90 100
LANSSSYHTF LLDEERSRLY VGAKDHIFSF NLVNIKDFQK IVWPVSYTRR
110 120 130 140 150
DECKWAGKDI LKECANFIKV LEAYNQTHLY ACGTGAFHPI CTYIEVGHHP
160 170 180 190 200
EDNIFKLQDS HFENGRGKSP YDPKLLTASL LIDGELYSGT AADFMGRDFA
210 220 230 240 250
IFRTLGHHHP IRTEQHDSRW LNDPRFISAH LIPESDNPED DKVYFFFREN
260 270 280 290 300
AIDGEHSGKA THARIGQICK NDFGGHRSLV NKWTTFLKAR LICSVPGPNG
310 320 330 340 350
IDTHFDELQD VFLMNSKDPK NPIVYGVFTT SSNIFKGSAV CMYSMSDVRR
360 370 380 390 400
VFLGPYAHRD GPNYQWVPYQ GRVPYPRPGT CPSKTFGGFD STKDLPDDVI
410 420 430 440 450
TFARSHPAMY NPVFPINNRP IMIKTDVNYQ FTQIVVDRVD AEDGQYDVMF
460 470 480 490 500
IGTDVGTVLK VVSVPKETWH DLEEILLEEM TVFREPTTIS AMELSTKQQQ
510 520 530 540 550
LYIGSTAGVA QLPLHRCDIY GKACAECCLA RDPYCAWDGS SCSRYFPTAK
560 570 580 590 600
RRTRRQDIRN GDPLTHCSDL QHHDNHHGPS LEERIIYGVE NSSTFLECSP
610 620 630 640 650
KSQRALVYWQ FQRRNEDRKE EIRMGDHIIR TEQGLLLRSL QKKDSGNYLC
660 670 680 690 700
HAVEHGFMQT LLKVTLEVID TEHLEELLHK DDDGDGSKIK EMSSSMTPSQ
710 720 730 740 750
KVWYRDFMQL INHPNLNTMD EFCEQVWKRD RKQRRQRPGH SQGSSNKWKH
760 770
MQESKKGRNR RTHEFERAPR SV
Length:772
Mass (Da):88,813
Last modified:July 27, 2011 - v2
Checksum:iC6533FF007018D0C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q668E9Q668_MOUSE
Semaphorin-3A
Sema3a
688Annotation score:
D3Z2A7D3Z2A7_MOUSE
Semaphorin-3A
Sema3a
72Annotation score:
D3Z4Q3D3Z4Q3_MOUSE
Semaphorin-3A
Sema3a
62Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti193D → N in AAA73934 (PubMed:7748562).Curated1
Sequence conflicti207H → D in CAA59985 (PubMed:7748561).Curated1
Sequence conflicti253D → G in CAA59985 (PubMed:7748561).Curated1
Sequence conflicti352F → L in AAA73934 (PubMed:7748562).Curated1
Sequence conflicti403A → G in CAA59985 (PubMed:7748561).Curated1
Sequence conflicti475I → V in CAA59985 (PubMed:7748561).Curated1
Sequence conflicti475I → V in BAA19773 (PubMed:9331345).Curated1
Sequence conflicti475I → V in AAL77611 (Ref. 3) Curated1
Sequence conflicti475I → V in AAH57588 (PubMed:15489334).Curated1
Sequence conflicti475I → V in AAH90844 (PubMed:15489334).Curated1
Sequence conflicti475I → V in AAA73934 (PubMed:7748562).Curated1
Sequence conflicti571 – 572QH → ED in CAA59985 (PubMed:7748561).Curated2
Sequence conflicti616 – 620EDRKE → RRSKR in CAA59985 (PubMed:7748561).Curated5
Sequence conflicti623R → K in AAA73934 (PubMed:7748562).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85993 mRNA Translation: CAA59985.1
D85028 mRNA Translation: BAA19773.1
L41541 mRNA Translation: AAL77611.1
AC022368 Genomic DNA No translation available.
AC109165 Genomic DNA No translation available.
AC121125 Genomic DNA No translation available.
AC121841 Genomic DNA No translation available.
AC159971 Genomic DNA No translation available.
BC057588 mRNA Translation: AAH57588.1
BC090844 mRNA Translation: AAH90844.1
L40484 mRNA Translation: AAA73934.1
CCDSiCCDS19092.1
PIRiI48747
I58169
RefSeqiNP_001230001.1, NM_001243072.1
NP_001230002.1, NM_001243073.1
NP_033178.2, NM_009152.4
XP_006503620.1, XM_006503557.3
XP_006503621.1, XM_006503558.3
XP_006503622.1, XM_006503559.1
XP_011238967.1, XM_011240665.2
UniGeneiMm.372039
Mm.483774

Genome annotation databases

EnsembliENSMUST00000030714; ENSMUSP00000030714; ENSMUSG00000028883
ENSMUST00000095012; ENSMUSP00000092621; ENSMUSG00000028883
GeneIDi20346
KEGGimmu:20346
UCSCiuc008wmb.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85993 mRNA Translation: CAA59985.1
D85028 mRNA Translation: BAA19773.1
L41541 mRNA Translation: AAL77611.1
AC022368 Genomic DNA No translation available.
AC109165 Genomic DNA No translation available.
AC121125 Genomic DNA No translation available.
AC121841 Genomic DNA No translation available.
AC159971 Genomic DNA No translation available.
BC057588 mRNA Translation: AAH57588.1
BC090844 mRNA Translation: AAH90844.1
L40484 mRNA Translation: AAA73934.1
CCDSiCCDS19092.1
PIRiI48747
I58169
RefSeqiNP_001230001.1, NM_001243072.1
NP_001230002.1, NM_001243073.1
NP_033178.2, NM_009152.4
XP_006503620.1, XM_006503557.3
XP_006503621.1, XM_006503558.3
XP_006503622.1, XM_006503559.1
XP_011238967.1, XM_011240665.2
UniGeneiMm.372039
Mm.483774

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q47X-ray2.80A/B26-520[»]
4GZ8X-ray3.30A/B21-569[»]
4GZAX-ray7.00G21-555[»]
ProteinModelPortaliO08665
SMRiO08665
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203161, 2 interactors
DIPiDIP-59997N
IntActiO08665, 2 interactors
MINTiO08665
STRINGi10090.ENSMUSP00000030714

PTM databases

iPTMnetiO08665
PhosphoSitePlusiO08665

Proteomic databases

MaxQBiO08665
PaxDbiO08665
PeptideAtlasiO08665
PRIDEiO08665

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030714; ENSMUSP00000030714; ENSMUSG00000028883
ENSMUST00000095012; ENSMUSP00000092621; ENSMUSG00000028883
GeneIDi20346
KEGGimmu:20346
UCSCiuc008wmb.3 mouse

Organism-specific databases

CTDi10371
MGIiMGI:107558 Sema3a

Phylogenomic databases

eggNOGiKOG3611 Eukaryota
ENOG410XQZC LUCA
GeneTreeiENSGT00760000118854
HOGENOMiHOG000039964
HOVERGENiHBG055071
InParanoidiO08665
KOiK06840
OMAiREPTTIS
OrthoDBiEOG091G01W0
TreeFamiTF316102

Enzyme and pathway databases

ReactomeiR-MMU-399954 Sema3A PAK dependent Axon repulsion
R-MMU-399955 SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
R-MMU-399956 CRMPs in Sema3A signaling

Miscellaneous databases

EvolutionaryTraceiO08665
PROiPR:O08665
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028883 Expressed in 279 organ(s), highest expression level in retina
ExpressionAtlasiO08665 baseline and differential
GenevisibleiO08665 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR016201 PSI
IPR001627 Semap_dom
IPR036352 Semap_dom_sf
IPR027231 Semaphorin
IPR015943 WD40/YVTN_repeat-like_dom_sf
PANTHERiPTHR11036 PTHR11036, 1 hit
PfamiView protein in Pfam
PF01403 Sema, 1 hit
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00423 PSI, 1 hit
SM00630 Sema, 1 hit
SUPFAMiSSF101912 SSF101912, 1 hit
SSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51004 SEMA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSEM3A_MOUSE
AccessioniPrimary (citable) accession number: O08665
Secondary accession number(s): E9QK85
, Q5BL08, Q62180, Q62215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again