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Protein

Methionine aminopeptidase 2

Gene

Metap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei231SubstrateUniRule annotation1
Metal bindingi251Divalent metal cation 1UniRule annotation1
Metal bindingi262Divalent metal cation 1UniRule annotation1
Metal bindingi262Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi331Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei339SubstrateUniRule annotation1
Metal bindingi364Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi459Divalent metal cation 1UniRule annotation1
Metal bindingi459Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Enzyme and pathway databases

BRENDAi3.4.11.18 3474
ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Protein family/group databases

MEROPSiM24.002

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Initiation factor 2-associated 67 kDa glycoproteinUniRule annotation
Short name:
p67UniRule annotation
Short name:
p67eIF2UniRule annotation
Peptidase MUniRule annotation
Gene namesi
Name:Metap2
Synonyms:Mnpep, P67eif2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1929701 Metap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075272

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001489832 – 478Methionine aminopeptidase 2Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei60Phosphoserine; alternateBy similarity1
Glycosylationi60O-linked (GlcNAc) serine; alternateBy similarity1

Post-translational modificationi

Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO08663
PaxDbiO08663
PeptideAtlasiO08663
PRIDEiO08663

PTM databases

iPTMnetiO08663
PhosphoSitePlusiO08663
SwissPalmiO08663

Expressioni

Gene expression databases

BgeeiENSMUSG00000036112 Expressed in 258 organ(s), highest expression level in bone marrow
CleanExiMM_METAP2
ExpressionAtlasiO08663 baseline and differential
GenevisibleiO08663 MM

Interactioni

Subunit structurei

Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3.UniRule annotation

Protein-protein interaction databases

BioGridi207891, 5 interactors
IntActiO08663, 1 interactor
MINTiO08663
STRINGi10090.ENSMUSP00000048285

Chemistry databases

BindingDBiO08663

Structurei

3D structure databases

ProteinModelPortaliO08663
SMRiO08663
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 46Poly-Lys6
Compositional biasi98 – 106Poly-Lys9

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775 Eukaryota
COG0024 LUCA
GeneTreeiENSGT00530000063220
HOVERGENiHBG050495
InParanoidiO08663
KOiK01265
PhylomeDBiO08663
TreeFamiTF300426

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03175 MetAP_2_euk, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

O08663-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGVEQAASF GGHLNGDLDP DDREEGTSST AEEAAKKKRR KKKKGKGAVS
60 70 80 90 100
AVQQELDKES GALVDEVAKQ LESQALEEKE RDDDDEDGDG DADGATGKKK
110 120 130 140 150
KKKKKKRGPK VQTDPPSVPI CDLYPNGVFP KGQECEYPPT QDGRTAAWRT
160 170 180 190 200
TSEEKKALDQ ASEEIWNDFR EAAEAHRQVR KYVMSWIKPG MTMIEICEKL
210 220 230 240 250
EDCSRKLIKE NGLNAGLAFP TGCSLNNCAA HYTPNAGDTT VLQYDDICKI
260 270 280 290 300
DFGTHISGRI IDCAFTVTFN PKYDILLTAV KDATNTGIKC AGIDVRLCDV
310 320 330 340 350
GEAIQEVMES YEVEIDGKTY QVKPIRNLNG HSIGPYRIHA GKTVPIVKGG
360 370 380 390 400
EATRMEEGEV YAIETFGSTG KGVVHDDMEC SHYMKNFDVG HVPIRLPRTK
410 420 430 440 450
HLLNVINENF GTLAFCRRWL DRLGESKYLM ALKNLCDLGI VDPYPPLCDI
460 470
KGSYTAQFEH TILLRPTCKE VVSRGDDY
Length:478
Mass (Da):52,922
Last modified:July 1, 1997 - v1
Checksum:iBBB9A2AFC19952E8
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UI33Q3UI33_MOUSE
Methionine aminopeptidase 2
Metap2 METAP2
488Annotation score:
Q3TMN1Q3TMN1_MOUSE
Methionine aminopeptidase 2
Metap2 METAP2
455Annotation score:
M0QWX0M0QWX0_MOUSE
Methionine aminopeptidase 2
Metap2
106Annotation score:
M0QWM9M0QWM9_MOUSE
Methionine aminopeptidase 2
Metap2
242Annotation score:
M0QW69M0QW69_MOUSE
Methionine aminopeptidase 2
Metap2
186Annotation score:
M0QW48M0QW48_MOUSE
Methionine aminopeptidase 2
Metap2
233Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003144 mRNA Translation: BAA19789.1
AK076804 mRNA Translation: BAC36488.1
BC002213 mRNA Translation: AAH02213.1
CCDSiCCDS48673.1
RefSeqiNP_062622.1, NM_019648.3
XP_006513959.1, XM_006513896.3
XP_006513960.1, XM_006513897.3
UniGeneiMm.289329

Genome annotation databases

EnsembliENSMUST00000047910; ENSMUSP00000048285; ENSMUSG00000036112
ENSMUST00000180840; ENSMUSP00000138006; ENSMUSG00000036112
GeneIDi56307
KEGGimmu:56307
UCSCiuc007gve.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003144 mRNA Translation: BAA19789.1
AK076804 mRNA Translation: BAC36488.1
BC002213 mRNA Translation: AAH02213.1
CCDSiCCDS48673.1
RefSeqiNP_062622.1, NM_019648.3
XP_006513959.1, XM_006513896.3
XP_006513960.1, XM_006513897.3
UniGeneiMm.289329

3D structure databases

ProteinModelPortaliO08663
SMRiO08663
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207891, 5 interactors
IntActiO08663, 1 interactor
MINTiO08663
STRINGi10090.ENSMUSP00000048285

Chemistry databases

BindingDBiO08663
ChEMBLiCHEMBL1075272

Protein family/group databases

MEROPSiM24.002

PTM databases

iPTMnetiO08663
PhosphoSitePlusiO08663
SwissPalmiO08663

Proteomic databases

EPDiO08663
PaxDbiO08663
PeptideAtlasiO08663
PRIDEiO08663

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047910; ENSMUSP00000048285; ENSMUSG00000036112
ENSMUST00000180840; ENSMUSP00000138006; ENSMUSG00000036112
GeneIDi56307
KEGGimmu:56307
UCSCiuc007gve.1 mouse

Organism-specific databases

CTDi10988
MGIiMGI:1929701 Metap2

Phylogenomic databases

eggNOGiKOG2775 Eukaryota
COG0024 LUCA
GeneTreeiENSGT00530000063220
HOVERGENiHBG050495
InParanoidiO08663
KOiK01265
PhylomeDBiO08663
TreeFamiTF300426

Enzyme and pathway databases

BRENDAi3.4.11.18 3474
ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Miscellaneous databases

ChiTaRSiMetap2 mouse
PROiPR:O08663
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036112 Expressed in 258 organ(s), highest expression level in bone marrow
CleanExiMM_METAP2
ExpressionAtlasiO08663 baseline and differential
GenevisibleiO08663 MM

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03175 MetAP_2_euk, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAP2_MOUSE
AccessioniPrimary (citable) accession number: O08663
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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