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Protein

Hyaluronan synthase 3

Gene

Has3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction.1 Publication

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

Cofactori

Kineticsi

  1. KM=0.2 mM for UDP-Glc-NAc (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication
  2. KM=0.3 mM for UDP-Glc-UA (at pH 7.1 and 37 degrees Celsius, in the presence of 15 mM MgCl2)1 Publication

    Pathwayi: hyaluronan biosynthesis

    This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

    GO - Molecular functioni

    • hyaluronan synthase activity Source: UniProtKB
    • identical protein binding Source: MGI

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.212 3474
    ReactomeiR-MMU-2142850 Hyaluronan biosynthesis and export
    UniPathwayi
    UPA00341

    Protein family/group databases

    CAZyiGT2 Glycosyltransferase Family 2

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronan synthase 3 (EC:2.4.1.212)
    Alternative name(s):
    Hyaluronate synthase 3
    Hyaluronic acid synthase 3
    Short name:
    HA synthase 3
    Gene namesi
    Name:Has3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:109599 Has3

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
    Transmembranei16 – 36Helical; Name=1Sequence analysisAdd BLAST21
    Topological domaini37 – 44ExtracellularSequence analysis8
    Transmembranei45 – 65Helical; Name=2Sequence analysisAdd BLAST21
    Topological domaini66 – 378CytoplasmicSequence analysisAdd BLAST313
    Transmembranei379 – 399Helical; Name=3Sequence analysisAdd BLAST21
    Topological domaini400 – 409ExtracellularSequence analysis10
    Transmembranei410 – 430Helical; Name=4Sequence analysisAdd BLAST21
    Topological domaini431 – 441CytoplasmicSequence analysisAdd BLAST11
    Transmembranei442 – 462Helical; Name=5Sequence analysisAdd BLAST21
    Topological domaini463 – 474ExtracellularSequence analysisAdd BLAST12
    Transmembranei475 – 495Helical; Name=6Sequence analysisAdd BLAST21
    Topological domaini496 – 516CytoplasmicSequence analysisAdd BLAST21
    Transmembranei517 – 537Helical; Name=7Sequence analysisAdd BLAST21
    Topological domaini538 – 554ExtracellularSequence analysisAdd BLAST17

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001971791 – 554Hyaluronan synthase 3Add BLAST554

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi463N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO08650
    PRIDEiO08650

    PTM databases

    PhosphoSitePlusiO08650

    Expressioni

    Developmental stagei

    Expressed at E17.5 day.

    Gene expression databases

    BgeeiENSMUSG00000031910 Expressed in 94 organ(s), highest expression level in secondary oocyte
    CleanExiMM_HAS3
    ExpressionAtlasiO08650 baseline and differential
    GenevisibleiO08650 MM

    Interactioni

    GO - Molecular functioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034385

    Structurei

    3D structure databases

    ProteinModelPortaliO08650
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NodC/HAS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2571 Eukaryota
    COG1215 LUCA
    GeneTreeiENSGT00390000010337
    HOGENOMiHOG000112847
    HOVERGENiHBG000189
    InParanoidiO08650
    KOiK00752
    OMAiPTRYLRW
    OrthoDBiEOG091G04P3
    TreeFamiTF332506

    Family and domain databases

    InterProiView protein in InterPro
    IPR026108 HA_synthase_3
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR22913:SF6 PTHR22913:SF6, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

    O08650-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPVQLTTALR VVGTSLFALV VLGGILAAYV TGYQFIHTEK HYLSFGLYGA
    60 70 80 90 100
    ILGLHLLIQS LFAFLEHRRM RRAGRPLKLH CSQRPRSVAL CIAAYQEDPE
    110 120 130 140 150
    YLRKCLRSAQ RIAFPNLKVV MVVDGNRQED TYMLDIFHEV LGGTEQAGFF
    160 170 180 190 200
    VWRSNFHEAG EGETEASLQE GMERVRAVVW ASTFSCIMQK WGGKREVMYT
    210 220 230 240 250
    AFKALGNSVD YIQVCDSDTV LDPACTIEML RVLEEDPQVG GVGGDVQILN
    260 270 280 290 300
    KYDSWISFLS SVRYWMAFNV ERACQSYFGC VQCISGPLGM YRNSLLQQFL
    310 320 330 340 350
    EDWYHQKFLG SKCSFGDDRH LTNRVLSLGY RTKYTARSKC LTETPTRYLR
    360 370 380 390 400
    WLNQQTRWSK SYFREWLYNS LWFHKHHLWM TYESVVTGFF PFFLIATVIQ
    410 420 430 440 450
    LFYRGRIWNI LLFLLTVQLV GIIKATYACF LRGNAEMIFM SLYSLLYMSS
    460 470 480 490 500
    LLPAKIFAIA TINKSGWGTS GRKTIVVNFI GLIPVSIWVA VLLGGLAYTA
    510 520 530 540 550
    YCQDLFSETE LAFLVSGAIL YGCYWVALLM LYLAIIARRC GKKPEQYSLA

    FAEV
    Length:554
    Mass (Da):63,349
    Last modified:July 27, 2011 - v2
    Checksum:iBFFF239C901251DE
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    H3BL01H3BL01_MOUSE
    Hyaluronan synthase 3
    Has3
    258Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti85P → S in AAC53128 (PubMed:9083017).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U86408 mRNA Translation: AAC53128.1
    AK028582 mRNA Translation: BAC26017.1
    BC138152 mRNA Translation: AAI38153.1
    CCDSiCCDS22640.1
    RefSeqiNP_001317977.1, NM_001331048.1
    NP_032243.2, NM_008217.4
    XP_006530763.1, XM_006530700.3
    XP_006530764.1, XM_006530701.2
    UniGeneiMm.56986

    Genome annotation databases

    EnsembliENSMUST00000034385; ENSMUSP00000034385; ENSMUSG00000031910
    ENSMUST00000176144; ENSMUSP00000135303; ENSMUSG00000031910
    GeneIDi15118
    KEGGimmu:15118
    UCSCiuc009ngn.2 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U86408 mRNA Translation: AAC53128.1
    AK028582 mRNA Translation: BAC26017.1
    BC138152 mRNA Translation: AAI38153.1
    CCDSiCCDS22640.1
    RefSeqiNP_001317977.1, NM_001331048.1
    NP_032243.2, NM_008217.4
    XP_006530763.1, XM_006530700.3
    XP_006530764.1, XM_006530701.2
    UniGeneiMm.56986

    3D structure databases

    ProteinModelPortaliO08650
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034385

    Protein family/group databases

    CAZyiGT2 Glycosyltransferase Family 2

    PTM databases

    PhosphoSitePlusiO08650

    Proteomic databases

    PaxDbiO08650
    PRIDEiO08650

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000034385; ENSMUSP00000034385; ENSMUSG00000031910
    ENSMUST00000176144; ENSMUSP00000135303; ENSMUSG00000031910
    GeneIDi15118
    KEGGimmu:15118
    UCSCiuc009ngn.2 mouse

    Organism-specific databases

    CTDi3038
    MGIiMGI:109599 Has3

    Phylogenomic databases

    eggNOGiKOG2571 Eukaryota
    COG1215 LUCA
    GeneTreeiENSGT00390000010337
    HOGENOMiHOG000112847
    HOVERGENiHBG000189
    InParanoidiO08650
    KOiK00752
    OMAiPTRYLRW
    OrthoDBiEOG091G04P3
    TreeFamiTF332506

    Enzyme and pathway databases

    UniPathwayi
    UPA00341

    BRENDAi2.4.1.212 3474
    ReactomeiR-MMU-2142850 Hyaluronan biosynthesis and export

    Miscellaneous databases

    PROiPR:O08650
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000031910 Expressed in 94 organ(s), highest expression level in secondary oocyte
    CleanExiMM_HAS3
    ExpressionAtlasiO08650 baseline and differential
    GenevisibleiO08650 MM

    Family and domain databases

    InterProiView protein in InterPro
    IPR026108 HA_synthase_3
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR22913:SF6 PTHR22913:SF6, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHYAS3_MOUSE
    AccessioniPrimary (citable) accession number: O08650
    Secondary accession number(s): Q8CEB9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: November 7, 2018
    This is version 135 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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