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Protein

Ephrin type-B receptor 6

Gene

Ephb6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi669 – 677ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 6
Alternative name(s):
MEP
Tyrosine-protein kinase-defective receptor EPH-6
Gene namesi
Name:Ephb6
Synonyms:Cekl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1096338 Ephb6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 591ExtracellularSequence analysisAdd BLAST559
Transmembranei592 – 612HelicalSequence analysisAdd BLAST21
Topological domaini613 – 1014CytoplasmicSequence analysisAdd BLAST402

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi1834

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000001683833 – 1014Ephrin type-B receptor 6Add BLAST982

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi473N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion (By similarity).By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO08644
PaxDbiO08644
PeptideAtlasiO08644
PRIDEiO08644

PTM databases

iPTMnetiO08644
PhosphoSitePlusiO08644

Expressioni

Tissue specificityi

High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000029869 Expressed in 165 organ(s), highest expression level in zone of skin
CleanExiMM_EPHB6
ExpressionAtlasiO08644 baseline and differential
GenevisibleiO08644 MM

Interactioni

Subunit structurei

Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110380

Structurei

3D structure databases

ProteinModelPortaliO08644
SMRiO08644
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 232Eph LBDPROSITE-ProRule annotationAdd BLAST199
Domaini364 – 479Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST116
Domaini480 – 575Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini663 – 912Protein kinasePROSITE-ProRule annotationAdd BLAST250
Domaini941 – 1005SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1012 – 1014PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi168 – 173Poly-Ser6
Compositional biasi214 – 361Cys-richAdd BLAST148
Compositional biasi874 – 877Poly-Pro4

Domaini

The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2 (By similarity).PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00930000150909
HOGENOMiHOG000233856
HOVERGENiHBG062180
InParanoidiO08644
KOiK05114
OMAiFSKFGLC
OrthoDBiEOG091G00W0
PhylomeDBiO08644
TreeFamiTF314013

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF07699 Ephrin_rec_like, 1 hit
PF00041 fn3, 2 hits
PF07714 Pkinase_Tyr, 1 hit
PF07647 SAM_2, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O08644-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATEGTTGSG SRVVAGMVCS LWLLVLGSSV LALEEVLLDT TGETSEIGWL
60 70 80 90 100
TYPPGGWDEV SVLDDQRRLT RTFEACHVAG LPPGSGQDNW LQTHFVERRG
110 120 130 140 150
AQRAHIRLHF SVRACSSLGV SGGTCRETFT LYYRQADEPD GPDSIAAWHL
160 170 180 190 200
KRWTKVDTIA ADESFPASSS SSSWAVGPHR TGQRVGLQLN VKERSFGPLT
210 220 230 240 250
QRGFYVAFQD TGACLALVAV KLFSYTCPSV LRAFASFPET QASGAGGASL
260 270 280 290 300
VAAVGTCVAH AEPEEDGVGG QAGGSPPRLH CNGEGRWMVA VGGCRCQPGH
310 320 330 340 350
QPARGDKLCQ ACPEGSYKAL AGNVPCSPCP ARSHSPDPAA PVCPCLQGFY
360 370 380 390 400
RASSDPPEAP CTGPPSAPRE LWFEVQGSAL MLHWRLPQEL GGRGDLLFNV
410 420 430 440 450
VCKECGGHGE PSSGGMCRRC RDEVHFDPRQ RGLTESRVLV GGLRAHVPYI
460 470 480 490 500
LEVQAVNGVS ELSPDPPQAA AINVSTSHEV PSAVPVMHQV SRAANSITVS
510 520 530 540 550
WPQPEQTNGN ILDYQLRYYD QAEDESHSFT MTSETNTATV TRLSPGHIYG
560 570 580 590 600
FQVRARTAAG HGPYGGKVYF QTLPQGELSS QLPEKLSLVI GSILGALAFL
610 620 630 640 650
LLAAITVLAV IFQRKRRGTG YTEQLQQYSS PGLGVKYYID PSTYDDPCQA
660 670 680 690 700
IRELAREVDP TYIKIEEVIG AGSFGEVRRG RLQPRGRREQ AVAIQALWAG
710 720 730 740 750
GAESLKMTFL GRAALLGQFQ HPNILRLEGV VTKSRPVMVL TELMELGPLD
760 770 780 790 800
SFLRQREGQF SSLQLVAMQR GVAAAMQYLS SFAFVHRALS ARSVLVNSHL
810 820 830 840 850
VCKVARLGHS PQGSSSLLRW AAPEVITHGK YTTSSDVWSF GILMWEVMSY
860 870 880 890 900
GERPYWDMNE QEVLNAIEQE FRLPPPPGCP PGLHLLMLDT WQKDRARRPH
910 920 930 940 950
FDQLVAAFDK MIRKPDTLQA EGGSGDRPSQ ALLNPVALDF PCLDSPQAWL
960 970 980 990 1000
SAIGLECYQD NFSKFGLSTF SDVAQLSLED LPGLGITLAG HQKKLLHNIQ
1010
LLQQHLRQPG SVEV
Length:1,014
Mass (Da):110,107
Last modified:April 20, 2010 - v4
Checksum:i1091796E1F68A842
GO
Isoform 2 (identifier: O08644-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-328: P → PCPALPLFTEHSRPEVC
     329-1014: Missing.

Show »
Length:344
Mass (Da):36,465
Checksum:i58A33AC4FD2DC032
GO
Isoform 3 (identifier: O08644-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     583-583: P → S
     584-1014: Missing.

Show »
Length:583
Mass (Da):62,298
Checksum:i08E5C159BE0F376A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6TDC5F6TDC5_MOUSE
Ephrin type-B receptor 6
Ephb6
130Annotation score:
F6W657F6W657_MOUSE
Ephrin type-B receptor 6
Ephb6
46Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti527H → Y in BAC39868 (PubMed:16141072).Curated1
Sequence conflicti544S → T in AAH31924 (PubMed:15489334).Curated1
Sequence conflicti732T → A in AAB51430 (PubMed:8761299).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003020328P → PCPALPLFTEHSRPEVC in isoform 2. 1 Publication1
Alternative sequenceiVSP_003021329 – 1014Missing in isoform 2. 1 PublicationAdd BLAST686
Alternative sequenceiVSP_003022583P → S in isoform 3. 1 Publication1
Alternative sequenceiVSP_003023584 – 1014Missing in isoform 3. 1 PublicationAdd BLAST431

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77867 mRNA Translation: AAB51430.1
AK087423 mRNA Translation: BAC39868.1
AK163823 mRNA Translation: BAE37507.1
BC031924 mRNA Translation: AAH31924.1
CCDSiCCDS20051.1 [O08644-1]
RefSeqiNP_001139823.1, NM_001146351.1 [O08644-1]
NP_031706.3, NM_007680.4 [O08644-1]
UniGeneiMm.271976

Genome annotation databases

EnsembliENSMUST00000114732; ENSMUSP00000110380; ENSMUSG00000029869 [O08644-1]
GeneIDi13848
KEGGimmu:13848
UCSCiuc009bqa.2 mouse [O08644-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77867 mRNA Translation: AAB51430.1
AK087423 mRNA Translation: BAC39868.1
AK163823 mRNA Translation: BAE37507.1
BC031924 mRNA Translation: AAH31924.1
CCDSiCCDS20051.1 [O08644-1]
RefSeqiNP_001139823.1, NM_001146351.1 [O08644-1]
NP_031706.3, NM_007680.4 [O08644-1]
UniGeneiMm.271976

3D structure databases

ProteinModelPortaliO08644
SMRiO08644
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110380

Chemistry databases

GuidetoPHARMACOLOGYi1834

PTM databases

iPTMnetiO08644
PhosphoSitePlusiO08644

Proteomic databases

MaxQBiO08644
PaxDbiO08644
PeptideAtlasiO08644
PRIDEiO08644

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114732; ENSMUSP00000110380; ENSMUSG00000029869 [O08644-1]
GeneIDi13848
KEGGimmu:13848
UCSCiuc009bqa.2 mouse [O08644-1]

Organism-specific databases

CTDi2051
MGIiMGI:1096338 Ephb6

Phylogenomic databases

eggNOGiKOG0196 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00930000150909
HOGENOMiHOG000233856
HOVERGENiHBG062180
InParanoidiO08644
KOiK05114
OMAiFSKFGLC
OrthoDBiEOG091G00W0
PhylomeDBiO08644
TreeFamiTF314013

Miscellaneous databases

ChiTaRSiEphb6 mouse
PROiPR:O08644
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029869 Expressed in 165 organ(s), highest expression level in zone of skin
CleanExiMM_EPHB6
ExpressionAtlasiO08644 baseline and differential
GenevisibleiO08644 MM

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027936 Eph_TM
IPR001090 Ephrin_rcpt_lig-bd_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR013783 Ig-like_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR011641 Tyr-kin_ephrin_A/B_rcpt-like
IPR016257 Tyr_kinase_ephrin_rcpt
IPR001426 Tyr_kinase_rcpt_V_CS
PfamiView protein in Pfam
PF14575 EphA2_TM, 1 hit
PF01404 Ephrin_lbd, 1 hit
PF07699 Ephrin_rec_like, 1 hit
PF00041 fn3, 2 hits
PF07714 Pkinase_Tyr, 1 hit
PF07647 SAM_2, 1 hit
PIRSFiPIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00615 EPH_lbd, 1 hit
SM01411 Ephrin_rec_like, 1 hit
SM00060 FN3, 2 hits
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF49265 SSF49265, 1 hit
SSF49785 SSF49785, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51550 EPH_LBD, 1 hit
PS50853 FN3, 2 hits
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit
PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit
PS50105 SAM_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEPHB6_MOUSE
AccessioniPrimary (citable) accession number: O08644
Secondary accession number(s): Q3TQ77, Q8BN76, Q8K0A9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 20, 2010
Last modified: November 7, 2018
This is version 174 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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