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UniProtKB - O08638 (MYH11_MOUSE)

Protein

Myosin-11

Gene

Myh11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 185ATPSequence analysis8

GO - Molecular functioni

  • actin filament binding Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: UniProtKB-KW
  • motor activity Source: MGI
  • structural constituent of muscle Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-11
Alternative name(s):
Myosin heavy chain 11
Myosin heavy chain, smooth muscle isoform
SMMHC
Gene namesi
Name:Myh11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:102643 Myh11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234251 – 1972Myosin-11Add BLAST1972

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei23PhosphoserineBy similarity1
Modified residuei40PhosphoserineBy similarity1
Modified residuei129N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei1177PhosphothreonineBy similarity1
Modified residuei1684PhosphoserineBy similarity1
Modified residuei1722PhosphoserineBy similarity1
Modified residuei1951PhosphothreonineCombined sources1
Modified residuei1954PhosphoserineCombined sources1
Modified residuei1971PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiO08638
PaxDbiO08638
PeptideAtlasiO08638
PRIDEiO08638

PTM databases

iPTMnetiO08638
PhosphoSitePlusiO08638
SwissPalmiO08638

Expressioni

Gene expression databases

CleanExiMM_MYH11

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

IntActiO08638, 14 interactors
MINTiO08638
STRINGi10090.ENSMUSP00000087756

Structurei

3D structure databases

ProteinModelPortaliO08638
SMRiO08638
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 81Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST51
Domaini85 – 783Myosin motorPROSITE-ProRule annotationAdd BLAST699
Domaini786 – 815IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni661 – 683Actin-bindingBy similarityAdd BLAST23
Regioni762 – 776Actin-bindingBy similarityAdd BLAST15
Regioni1935 – 1972C-terminalAdd BLAST38

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili844 – 1934Sequence analysisAdd BLAST1091

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161 Eukaryota
COG5022 LUCA
HOGENOMiHOG000173958
HOVERGENiHBG004704
InParanoidiO08638
PhylomeDBiO08638

Family and domain databases

Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
4.10.270.10, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR027401 Myosin_IQ_contain_sf
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR002928 Myosin_tail
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF02736 Myosin_N, 1 hit
PF01576 Myosin_tail_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O08638-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQKGQLSDD EKFLFVDKNF MNSPMAQADW VAKKLVWVPS EKQGFEAASI 
        60         70         80         90        100
KEEKGDEVVV ELVENGKKVT VGKDDIQKMN PPKFSKVEDM AELTCLNEAS 
       110        120        130        140        150
VLHNLRERYF SGLIYTYSGL FCVVVNPYKY LPIYSEKIVD MYKGKKRHEM 
       160        170        180        190        200
PPHIYAIADT AYRSMLQDRE DQSILCTGES GAGKTENTQK VIQYLAVVAS 
       210        220        230        240        250
SHKGKKDSSI TGELEKQLLQ ANPILEAFGN AKTVKNDNSS RFGKFIRINF 
       260        270        280        290        300
DVTGYIVGAN IETYLLEKSR AIRQARDERT FHIFYYLLAG AKEKMKSDLL 
       310        320        330        340        350
LESFNSYTFL SNGFVPIPAA QDDEMFQETL EAMSIMGFNE EEQLAILKVV 
       360        370        380        390        400
SSVLQLGNIV FKKERNTDQA SMPDNTAAQK VCHLVGINVT DFTRAILTPR 
       410        420        430        440        450
IKVGRDVVQK AQTKEQADFA IEALAKATYE RLFRWILSRV NKALDKTHRQ 
       460        470        480        490        500
GASFLGILDI AGFEIFEVNS FEQLCINYTN EKLQQLFNHT MFILEQEEYQ 
       510        520        530        540        550
REGIEWNFID FGLDLQPSIE LIERPNNPPG VLALLDEECW FPKATDKSFV 
       560        570        580        590        600
EKLCSEQGNH PKFQKPKQLK DKTEFSIIHY AGKVDYNASA WLTKNMDPLN 
       610        620        630        640        650
DNVTSLLNAS SDKFVADLWK DVDRIVGLDQ MAKMTESSLP SASKTKKGMF 
       660        670        680        690        700
RTVGQLYKEQ LGKLMATLRN TTANFVRCII PNHEKRSGKL DAFLVLEQLR 
       710        720        730        740        750
CNGVLEGIRI CRQGFPNRIV FQEFRQRYEI LAANAIPKGF MDGKQACILM 
       760        770        780        790        800
IKALELDPNL YRIGQSKIFF RTGVLAHLEE ERDLKITDVI MAFQAMCRGY 
       810        820        830        840        850
LARKAFTKRQ QQLTAMKVIQ RNCAAYLKLR NWQWWRLFTK VKPLLQVTRQ 
       860        870        880        890        900
EEEMQAKEEE MQKITERQQK AETELKELEQ KHTQLAEEKT LLQEQLQAET 
       910        920        930        940        950
ELYAESEEMR VRLAAKKQEL EEILHEMEAR LEEEEDRRQQ LQAERKKMAQ 
       960        970        980        990       1000
QMLDLEEQLE EEEAARQKLQ LEKVTAEAKI KKLEDDILVM DDQNSKLSKE 
      1010       1020       1030       1040       1050
RKLLEERVSD LTTNLAEEEE KAKNLTKLKS KHESMISELE VRLKKEEKSR 
      1060       1070       1080       1090       1100
QELEKLKRKL EGDASDFHEQ IADLQAQIAE LKMQLAKKEE ELQAALARLD 
      1110       1120       1130       1140       1150
EEIAQKNNAL KKIRELEGHI SDLQEDLDSE RAARNKAEKQ KRDLGEELEA 
      1160       1170       1180       1190       1200
LKTELEDTLD STATQQELRA KREQEVTVLK KALDEETRSH EAQVQEMRQK 
      1210       1220       1230       1240       1250
HTQAVEELTE QLEQFKRAKA NLDKSKQTLE KENADLAGEL RVLGQAKQEV 
      1260       1270       1280       1290       1300
EHKKKKLEVQ LQDLQSKCSD GERARAELSD KVHKLQNEVE SVTGMLNEAE 
      1310       1320       1330       1340       1350
GKAIKLAKDV ASLGSQLQDT QELLQEETRQ KLNVSTKLRQ LEDERNSLQD 
      1360       1370       1380       1390       1400
QLDEEMEAKQ NLERHVSTLN IQLSDSKKKL QDFASTIEVM EEGKKRLQKE 
      1410       1420       1430       1440       1450
MEGLSQQYEE KAAAYDKLEK TKNRLQQELD DLVVDLDNQR QLVSNLEKKQ 
      1460       1470       1480       1490       1500
KKFDQLLAEE KNISSKYADE RDRAEAEARE KETKALSLAR ALEEALEAKE 
      1510       1520       1530       1540       1550
ELERTNKMLK AEMEDLVSSK DDVGKNVHEL EKSKRALETQ MEEMKTQLEE 
      1560       1570       1580       1590       1600
SEDDVQATED AKLRLEVNMQ ALKGQFERDL QARDEQNEEK RRQLQRQLHE 
      1610       1620       1630       1640       1650
YETELEDERK QRALAAAAKK KLEGDLKDLE LQADSAIKGR EEAIKQLRKL 
      1660       1670       1680       1690       1700
QAQMKDFQRE LDDARASRDE IFATSKENEK KAKSLEADLM QLQEDLAAAE 
      1710       1720       1730       1740       1750
RARKQADLEK EELAEELASS LSGRNTLQDE KRRLEARIAQ LEEELEEEQG 
      1760       1770       1780       1790       1800
NMEAMSDRVR KATLQAEQLS NELATERSTA QKNESARQQL ERQNKELRSK 
      1810       1820       1830       1840       1850
LQEVEGAVKA KLKSTVAALE AKIAQLEEQV EQEAREKQAA TKSLKQKDKK 
      1860       1870       1880       1890       1900
LKEVLLQVED ERKMAEQYKE QAEKGNTKVK QLKRQLEEAE EESQCINANR 
      1910       1920       1930       1940       1950
RKLQRELDEA TESNEAMGRE VNALKSKLRR GNEASFVPSR RAGGRRVIEN 
      1960       1970 
TDGSEEEMDA RDSDFNGTKA SE
Length:1,972
Mass (Da):227,028
Last modified:July 1, 1997 - v1
Checksum:iA1398E3F5B11F15A
GO
Isoform 2 (identifier: O08638-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1930-1972: RGNEASFVPSRRAGGRRVIENTDGSEEEMDARDSDFNGTKASE → GPPPQETSQ

Show »
Length:1,938
Mass (Da):223,266
Checksum:i46A0B1B9BCB63A8A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8VHF9A0A2R8VHF9_MOUSE
Myosin-11
Myh11
1,938Annotation score:
E9QPE7E9QPE7_MOUSE
Myosin-11
Myh11
1,972Annotation score:
A0A338P6K2A0A338P6K2_MOUSE
Myosin-11
Myh11
1,979Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126N → D in AAB36168 (PubMed:8593698).Curated1
Sequence conflicti161A → V in AAA67552 (PubMed:7923625).Curated1
Sequence conflicti189Q → K in AAA67552 (PubMed:7923625).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0033461930 – 1972RGNEA…TKASE → GPPPQETSQ in isoform 2. CuratedAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85923 mRNA Translation: BAA19690.1
D85924 mRNA Translation: BAA19691.1
L25860 mRNA Translation: AAA67552.1
S81516 mRNA Translation: AAB36168.1
CCDSiCCDS27972.1 [O08638-2]
PIRiI52863
JC5420
JC5421
UniGeneiMm.250705

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85923 mRNA Translation: BAA19690.1
D85924 mRNA Translation: BAA19691.1
L25860 mRNA Translation: AAA67552.1
S81516 mRNA Translation: AAB36168.1
CCDSiCCDS27972.1 [O08638-2]
PIRiI52863
JC5420
JC5421
UniGeneiMm.250705

3D structure databases

ProteinModelPortaliO08638
SMRiO08638
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08638, 14 interactors
MINTiO08638
STRINGi10090.ENSMUSP00000087756

PTM databases

iPTMnetiO08638
PhosphoSitePlusiO08638
SwissPalmiO08638

Proteomic databases

MaxQBiO08638
PaxDbiO08638
PeptideAtlasiO08638
PRIDEiO08638

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:102643 Myh11

Phylogenomic databases

eggNOGiKOG0161 Eukaryota
COG5022 LUCA
HOGENOMiHOG000173958
HOVERGENiHBG004704
InParanoidiO08638
PhylomeDBiO08638

Miscellaneous databases

ChiTaRSiMyh11 mouse
PROiPR:O08638
SOURCEiSearch...

Gene expression databases

CleanExiMM_MYH11

Family and domain databases

Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
4.10.270.10, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR027401 Myosin_IQ_contain_sf
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR002928 Myosin_tail
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF02736 Myosin_N, 1 hit
PF01576 Myosin_tail_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYH11_MOUSE
AccessioniPrimary (citable) accession number: O08638
Secondary accession number(s): O08639, Q62462, Q64195
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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