UniProtKB - O08638 (MYH11_MOUSE)
Protein
Myosin-11
Gene
Myh11
Organism
Mus musculus (Mouse)
Status
Functioni
Muscle contraction.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 178 – 185 | ATPSequence analysis | 8 |
GO - Molecular functioni
- actin filament binding Source: InterPro
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- motor activity Source: MGI
- structural constituent of muscle Source: UniProtKB
GO - Biological processi
- cardiac muscle fiber development Source: UniProtKB
- elastic fiber assembly Source: UniProtKB
- skeletal muscle myosin thick filament assembly Source: UniProtKB
- smooth muscle contraction Source: MGI
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-445355, Smooth Muscle Contraction R-MMU-5627123, RHO GTPases activate PAKs |
Names & Taxonomyi
Protein namesi | Recommended name: Myosin-11Alternative name(s): Myosin heavy chain 11 Myosin heavy chain, smooth muscle isoform SMMHC |
Gene namesi | Name:Myh11 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102643, Myh11 |
Subcellular locationi
Other locations
- Melanosome By similarity
- myofibril
Note: Thick filaments of the myofibrils.
Cytoskeleton
- muscle myosin complex Source: MGI
- myosin complex Source: MGI
- stress fiber Source: MGI
Other locations
- brush border Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
- myofibril Source: UniProtKB-SubCell
- myosin filament Source: UniProtKB-KW
- smooth muscle contractile fiber Source: MGI
Keywords - Cellular componenti
Cytoplasm, Thick filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123425 | 1 – 1972 | Myosin-11Add BLAST | 1972 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 8 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 40 | PhosphoserineBy similarity | 1 | |
Modified residuei | 129 | N6,N6,N6-trimethyllysineSequence analysis | 1 | |
Modified residuei | 1177 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1684 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1722 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1951 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1954 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1971 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | O08638 |
MaxQBi | O08638 |
PaxDbi | O08638 |
PeptideAtlasi | O08638 |
PRIDEi | O08638 |
PTM databases
iPTMneti | O08638 |
PhosphoSitePlusi | O08638 |
SwissPalmi | O08638 |
Interactioni
Subunit structurei
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
GO - Molecular functioni
- actin filament binding Source: InterPro
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
IntActi | O08638, 14 interactors |
MINTi | O08638 |
STRINGi | 10090.ENSMUSP00000087756 |
Miscellaneous databases
RNActi | O08638, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 81 | Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST | 51 | |
Domaini | 85 – 783 | Myosin motorPROSITE-ProRule annotationAdd BLAST | 699 | |
Domaini | 786 – 815 | IQPROSITE-ProRule annotationAdd BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 661 – 683 | Actin-bindingBy similarityAdd BLAST | 23 | |
Regioni | 762 – 776 | Actin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 1935 – 1972 | C-terminalAdd BLAST | 38 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 844 – 1934 | Sequence analysisAdd BLAST | 1091 |
Domaini
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0161, Eukaryota |
InParanoidi | O08638 |
PhylomeDBi | O08638 |
Family and domain databases
Gene3Di | 2.30.30.360, 1 hit 3.40.850.10, 1 hit 4.10.270.10, 1 hit |
InterProi | View protein in InterPro IPR000048, IQ_motif_EF-hand-BS IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR027401, Myosin_IQ_contain_sf IPR004009, Myosin_N IPR008989, Myosin_S1_N IPR002928, Myosin_tail IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00063, Myosin_head, 1 hit PF02736, Myosin_N, 1 hit PF01576, Myosin_tail_1, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50096, IQ, 1 hit PS51456, MYOSIN_MOTOR, 1 hit PS51844, SH3_LIKE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O08638-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQKGQLSDD EKFLFVDKNF MNSPMAQADW VAKKLVWVPS EKQGFEAASI
60 70 80 90 100
KEEKGDEVVV ELVENGKKVT VGKDDIQKMN PPKFSKVEDM AELTCLNEAS
110 120 130 140 150
VLHNLRERYF SGLIYTYSGL FCVVVNPYKY LPIYSEKIVD MYKGKKRHEM
160 170 180 190 200
PPHIYAIADT AYRSMLQDRE DQSILCTGES GAGKTENTQK VIQYLAVVAS
210 220 230 240 250
SHKGKKDSSI TGELEKQLLQ ANPILEAFGN AKTVKNDNSS RFGKFIRINF
260 270 280 290 300
DVTGYIVGAN IETYLLEKSR AIRQARDERT FHIFYYLLAG AKEKMKSDLL
310 320 330 340 350
LESFNSYTFL SNGFVPIPAA QDDEMFQETL EAMSIMGFNE EEQLAILKVV
360 370 380 390 400
SSVLQLGNIV FKKERNTDQA SMPDNTAAQK VCHLVGINVT DFTRAILTPR
410 420 430 440 450
IKVGRDVVQK AQTKEQADFA IEALAKATYE RLFRWILSRV NKALDKTHRQ
460 470 480 490 500
GASFLGILDI AGFEIFEVNS FEQLCINYTN EKLQQLFNHT MFILEQEEYQ
510 520 530 540 550
REGIEWNFID FGLDLQPSIE LIERPNNPPG VLALLDEECW FPKATDKSFV
560 570 580 590 600
EKLCSEQGNH PKFQKPKQLK DKTEFSIIHY AGKVDYNASA WLTKNMDPLN
610 620 630 640 650
DNVTSLLNAS SDKFVADLWK DVDRIVGLDQ MAKMTESSLP SASKTKKGMF
660 670 680 690 700
RTVGQLYKEQ LGKLMATLRN TTANFVRCII PNHEKRSGKL DAFLVLEQLR
710 720 730 740 750
CNGVLEGIRI CRQGFPNRIV FQEFRQRYEI LAANAIPKGF MDGKQACILM
760 770 780 790 800
IKALELDPNL YRIGQSKIFF RTGVLAHLEE ERDLKITDVI MAFQAMCRGY
810 820 830 840 850
LARKAFTKRQ QQLTAMKVIQ RNCAAYLKLR NWQWWRLFTK VKPLLQVTRQ
860 870 880 890 900
EEEMQAKEEE MQKITERQQK AETELKELEQ KHTQLAEEKT LLQEQLQAET
910 920 930 940 950
ELYAESEEMR VRLAAKKQEL EEILHEMEAR LEEEEDRRQQ LQAERKKMAQ
960 970 980 990 1000
QMLDLEEQLE EEEAARQKLQ LEKVTAEAKI KKLEDDILVM DDQNSKLSKE
1010 1020 1030 1040 1050
RKLLEERVSD LTTNLAEEEE KAKNLTKLKS KHESMISELE VRLKKEEKSR
1060 1070 1080 1090 1100
QELEKLKRKL EGDASDFHEQ IADLQAQIAE LKMQLAKKEE ELQAALARLD
1110 1120 1130 1140 1150
EEIAQKNNAL KKIRELEGHI SDLQEDLDSE RAARNKAEKQ KRDLGEELEA
1160 1170 1180 1190 1200
LKTELEDTLD STATQQELRA KREQEVTVLK KALDEETRSH EAQVQEMRQK
1210 1220 1230 1240 1250
HTQAVEELTE QLEQFKRAKA NLDKSKQTLE KENADLAGEL RVLGQAKQEV
1260 1270 1280 1290 1300
EHKKKKLEVQ LQDLQSKCSD GERARAELSD KVHKLQNEVE SVTGMLNEAE
1310 1320 1330 1340 1350
GKAIKLAKDV ASLGSQLQDT QELLQEETRQ KLNVSTKLRQ LEDERNSLQD
1360 1370 1380 1390 1400
QLDEEMEAKQ NLERHVSTLN IQLSDSKKKL QDFASTIEVM EEGKKRLQKE
1410 1420 1430 1440 1450
MEGLSQQYEE KAAAYDKLEK TKNRLQQELD DLVVDLDNQR QLVSNLEKKQ
1460 1470 1480 1490 1500
KKFDQLLAEE KNISSKYADE RDRAEAEARE KETKALSLAR ALEEALEAKE
1510 1520 1530 1540 1550
ELERTNKMLK AEMEDLVSSK DDVGKNVHEL EKSKRALETQ MEEMKTQLEE
1560 1570 1580 1590 1600
SEDDVQATED AKLRLEVNMQ ALKGQFERDL QARDEQNEEK RRQLQRQLHE
1610 1620 1630 1640 1650
YETELEDERK QRALAAAAKK KLEGDLKDLE LQADSAIKGR EEAIKQLRKL
1660 1670 1680 1690 1700
QAQMKDFQRE LDDARASRDE IFATSKENEK KAKSLEADLM QLQEDLAAAE
1710 1720 1730 1740 1750
RARKQADLEK EELAEELASS LSGRNTLQDE KRRLEARIAQ LEEELEEEQG
1760 1770 1780 1790 1800
NMEAMSDRVR KATLQAEQLS NELATERSTA QKNESARQQL ERQNKELRSK
1810 1820 1830 1840 1850
LQEVEGAVKA KLKSTVAALE AKIAQLEEQV EQEAREKQAA TKSLKQKDKK
1860 1870 1880 1890 1900
LKEVLLQVED ERKMAEQYKE QAEKGNTKVK QLKRQLEEAE EESQCINANR
1910 1920 1930 1940 1950
RKLQRELDEA TESNEAMGRE VNALKSKLRR GNEASFVPSR RAGGRRVIEN
1960 1970
TDGSEEEMDA RDSDFNGTKA SE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VHF9 | A0A2R8VHF9_MOUSE | Myosin-11 | Myh11 | 1,938 | Annotation score: | ||
A0A338P6K2 | A0A338P6K2_MOUSE | Myosin-11 | Myh11 | 1,979 | Annotation score: | ||
E9QPE7 | E9QPE7_MOUSE | Myosin-11 | Myh11 | 1,972 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 126 | N → D in AAB36168 (PubMed:8593698).Curated | 1 | |
Sequence conflicti | 161 | A → V in AAA67552 (PubMed:7923625).Curated | 1 | |
Sequence conflicti | 189 | Q → K in AAA67552 (PubMed:7923625).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003346 | 1930 – 1972 | RGNEA…TKASE → GPPPQETSQ in isoform 2. CuratedAdd BLAST | 43 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85923 mRNA Translation: BAA19690.1 D85924 mRNA Translation: BAA19691.1 L25860 mRNA Translation: AAA67552.1 S81516 mRNA Translation: AAB36168.1 |
CCDSi | CCDS27972.1 [O08638-2] CCDS88881.1 [O08638-1] |
PIRi | I52863 JC5420 JC5421 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85923 mRNA Translation: BAA19690.1 D85924 mRNA Translation: BAA19691.1 L25860 mRNA Translation: AAA67552.1 S81516 mRNA Translation: AAB36168.1 |
CCDSi | CCDS27972.1 [O08638-2] CCDS88881.1 [O08638-1] |
PIRi | I52863 JC5420 JC5421 |
3D structure databases
SMRi | O08638 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08638, 14 interactors |
MINTi | O08638 |
STRINGi | 10090.ENSMUSP00000087756 |
PTM databases
iPTMneti | O08638 |
PhosphoSitePlusi | O08638 |
SwissPalmi | O08638 |
Proteomic databases
jPOSTi | O08638 |
MaxQBi | O08638 |
PaxDbi | O08638 |
PeptideAtlasi | O08638 |
PRIDEi | O08638 |
Organism-specific databases
MGIi | MGI:102643, Myh11 |
Phylogenomic databases
eggNOGi | KOG0161, Eukaryota |
InParanoidi | O08638 |
PhylomeDBi | O08638 |
Enzyme and pathway databases
Reactomei | R-MMU-445355, Smooth Muscle Contraction R-MMU-5627123, RHO GTPases activate PAKs |
Miscellaneous databases
ChiTaRSi | Myh11, mouse |
PROi | PR:O08638 |
RNActi | O08638, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.30.30.360, 1 hit 3.40.850.10, 1 hit 4.10.270.10, 1 hit |
InterProi | View protein in InterPro IPR000048, IQ_motif_EF-hand-BS IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR027401, Myosin_IQ_contain_sf IPR004009, Myosin_N IPR008989, Myosin_S1_N IPR002928, Myosin_tail IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00063, Myosin_head, 1 hit PF02736, Myosin_N, 1 hit PF01576, Myosin_tail_1, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50096, IQ, 1 hit PS51456, MYOSIN_MOTOR, 1 hit PS51844, SH3_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYH11_MOUSE | |
Accessioni | O08638Primary (citable) accession number: O08638 Secondary accession number(s): O08639, Q62462, Q64195 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | July 1, 1997 | |
Last modified: | August 12, 2020 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families