UniProtKB - O08623 (SQSTM_RAT)
Sequestosome-1
Sqstm1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 164 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 46 |
GO - Molecular functioni
- enzyme binding Source: RGD
- identical protein binding Source: RGD
- ionotropic glutamate receptor binding Source: RGD
- K63-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- protein-containing complex binding Source: RGD
- protein kinase binding Source: RGD
- protein kinase C binding Source: RGD
- SH2 domain binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
- ubiquitin protein ligase binding Source: RGD
- zinc ion binding Source: InterPro
GO - Biological processi
- aggrephagy Source: UniProtKB
- apoptotic process Source: UniProtKB-KW
- autophagy Source: UniProtKB
- cell differentiation Source: UniProtKB-KW
- endosome organization Source: UniProtKB
- immune system process Source: UniProtKB-KW
- macroautophagy Source: UniProtKB
- mitochondrion organization Source: GO_Central
- mitophagy Source: RGD
- negative regulation of protein ubiquitination Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of long-term synaptic potentiation Source: RGD
- positive regulation of protein localization to plasma membrane Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- protein localization to perinuclear region of cytoplasm Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of protein complex stability Source: RGD
- response to ischemia Source: RGD
- response to mitochondrial depolarisation Source: RGD
- selective autophagy Source: UniProtKB
Keywordsi
Biological process | Apoptosis, Autophagy, Differentiation, Immunity |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-205043, NRIF signals cell death from the nucleus R-RNO-209543, p75NTR recruits signalling complexes R-RNO-209560, NF-kB is activated and signals survival R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-9020702, Interleukin-1 signaling R-RNO-9664873, Pexophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Sequestosome-1Alternative name(s): Protein kinase C-zeta-interacting protein Short name: PKC-zeta-interacting protein Ubiquitin-binding protein p62 |
Gene namesi | Name:Sqstm1 Synonyms:Zip |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69287, Sqstm1 |
Subcellular locationi
Nucleus
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Lysosome
- Lysosome By similarity
Endosome
- Late endosome By similarity
Cytosol
- cytosol By similarity
Other locations
- autophagosome By similarity
- sarcomere
Note: In cardiac muscles, localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Colocalizes with TRIM13 on the perinuclear endoplasmic reticulum (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates (By similarity). Co-localizes with TRIM5 in cytoplasmic bodies (By similarity). When nuclear export is blocked by treatment with leptomycin B, accumulates in PML bodies (By similarity).By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Endosome
- late endosome Source: UniProtKB-SubCell
Lysosome
- autolysosome Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Nucleus
- PML body Source: RGD
Other locations
- aggresome Source: RGD
- amphisome Source: RGD
- autophagosome Source: UniProtKB
- cytoplasm Source: RGD
- inclusion body Source: RGD
- Lewy body Source: RGD
- P-body Source: UniProtKB
- phagophore assembly site Source: RGD
- sarcomere Source: UniProtKB-SubCell
- sperm midpiece Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 7 | K → A: Loss of interaction with PRKCI. 1 Publication | 1 | |
Mutagenesisi | 21 – 22 | RR → AA: Reduces interaction with PRKCI. 1 Publication | 2 | |
Mutagenesisi | 67 | D → A: No effect on interaction with PRKCI. Abolishes homooligomerization; when associated with A-67. 2 Publications | 1 | |
Mutagenesisi | 69 | D → A: Abolishes homooligomerization; when associated with A-67. 1 Publication | 1 | |
Mutagenesisi | 94 | R → A: Reduces interaction with PRKCI. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000072179 | 2 – 439 | Sequestosome-1Add BLAST | 438 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 24 | PhosphoserineBy similarity | 1 | |
Modified residuei | 145 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 175 | PhosphoserineBy similarity | 1 | |
Modified residuei | 204 | PhosphoserineBy similarity | 1 | |
Modified residuei | 246 | PhosphoserineBy similarity | 1 | |
Modified residuei | 263 | PhosphoserineBy similarity | 1 | |
Modified residuei | 266 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 269 | PhosphoserineBy similarity | 1 | |
Modified residuei | 305 | PhosphoserineBy similarity | 1 | |
Modified residuei | 327 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Modified residuei | 348 | PhosphoserineBy similarity | 1 | |
Modified residuei | 354 | PhosphoserineCombined sources | 1 | |
Modified residuei | 360 | PhosphoserineBy similarity | 1 | |
Modified residuei | 364 | PhosphoserineBy similarity | 1 | |
Modified residuei | 365 | PhosphoserineBy similarity | 1 | |
Modified residuei | 402 | Phosphoserine; by ULK1 and TBK1By similarity | 1 | |
Cross-linki | 419 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 434 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | O08623 |
PaxDbi | O08623 |
PRIDEi | O08623 |
PTM databases
iPTMneti | O08623 |
PhosphoSitePlusi | O08623 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000003147, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | O08623, baseline and differential |
Genevisiblei | O08623, RN |
Interactioni
Subunit structurei
Homooligomer or heterooligomer; may form homotypic arrays.
Interacts directly with PRKCI and PRKCZ (Probable).
Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, TRIM13, MAP2K5 and MAPKAPK5.
Interacts with the proteasome subunits PSMD4 and PSMC2.
Interacts with K63-polyubiquitinated MAPT/TAU.
Interacts with IKBKB through PRKCZ and PRKCI.
Forms a complex with MAP2K5 and PRKCZ or PRKCI.
Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 probably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1.
Forms a complex with AJUBA, PRKCZ and TRAF6.
Interacts with TRAF6 and CYLD.
Identified in a complex with TRAF6 and CYLD.
Identified in a heterotrimeric complex with ubiquitin and ZFAND5, where ZFAND5 and SQSTM1 both interact with the same ubiquitin molecule (By similarity). Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3.
Interacts with KCNAB1, GABRR1, GABRR2 and GABRR3.
Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6.
Interacts with NGFR through TRAF6 and bridges that complex to NTRK1.
Interacts with NBR1 and TRIM55. Directly interacts with MAP1LC3A and MAP1LC3B, as well as with other MAP1 LC3 family members, including GABARAP, GABARAPL1 and GABARAPL2; these interactions are necessary for the recruitment MAP1 LC3 family members to inclusion bodies containing polyubiquitinated protein aggregates and for their degradation by autophagy (By similarity).
Interacts with FHOD3 (By similarity).
Interacts with TRMI5 (By similarity).
Interacts with SESN1 (By similarity).
Interacts with SESN2 (By similarity).
Interacts with ULK1.
Interacts with UBD (By similarity).
Interacts with WDR81; the interaction is direct and regulates the interaction of SQSTM1 with ubiquitinated proteins (By similarity).
Interacts with WDFY3; this interaction is required to recruit WDFY3 to cytoplasmic bodies and to PML bodies (By similarity).
Interacts with TRIM23 (By similarity).
Interacts with LRRC25 (By similarity).
Interacts with TRIM50 (By similarity).
Interacts with TRIM16 (By similarity).
Interacts with STING1; leading to relocalization of STING1 to autophagosomes (By similarity).
Interacts (when phosphorylated at Ser-348) with KEAP1; the interaction is direct and inactivates the BCR(KEAP1) complex by sequestering KEAP1 in inclusion bodies, promoting its degradation.
By similarityCuratedGO - Molecular functioni
- enzyme binding Source: RGD
- identical protein binding Source: RGD
- ionotropic glutamate receptor binding Source: RGD
- K63-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- protein kinase binding Source: RGD
- protein kinase C binding Source: RGD
- SH2 domain binding Source: UniProtKB
- ubiquitin binding Source: UniProtKB
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 250206, 31 interactors |
CORUMi | O08623 |
IntActi | O08623, 5 interactors |
MINTi | O08623 |
STRINGi | 10116.ENSRNOP00000056021 |
Structurei
Secondary structure
3D structure databases
BMRBi | O08623 |
SMRi | O08623 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O08623 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 100 | PB1PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 388 – 433 | UBAPROSITE-ProRule annotationAdd BLAST | 46 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 48 | Interaction with LCKBy similarityAdd BLAST | 47 | |
Regioni | 41 – 105 | Interaction with PRKCZ and dimerization2 PublicationsAdd BLAST | 65 | |
Regioni | 48 – 78 | Interaction with PAWRBy similarityAdd BLAST | 31 | |
Regioni | 119 – 221 | Interaction with GABRR31 PublicationAdd BLAST | 103 | |
Regioni | 167 – 217 | LIM protein-bindingBy similarityAdd BLAST | 51 | |
Regioni | 266 – 439 | Interaction with NTRK11 PublicationAdd BLAST | 174 | |
Regioni | 320 – 341 | MAP1LC3B-bindingBy similarityAdd BLAST | 22 | |
Regioni | 346 – 351 | Interaction with KEAP1By similarity | 6 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 225 – 230 | TRAF6-bindingBy similarity | 6 | |
Motifi | 335 – 340 | LIR | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 269 – 293 | Ser-richAdd BLAST | 25 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 164 | ZZ-typePROSITE-ProRule annotationAdd BLAST | 46 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4582, Eukaryota |
GeneTreei | ENSGT00390000002781 |
HOGENOMi | CLU_038011_1_0_1 |
InParanoidi | O08623 |
OMAi | WHPLQWF |
OrthoDBi | 1275680at2759 |
PhylomeDBi | O08623 |
Family and domain databases
CDDi | cd06402, PB1_p62, 1 hit cd14320, UBA_SQSTM, 1 hit |
Gene3Di | 3.30.60.90, 1 hit |
IDEALi | IID50133 |
InterProi | View protein in InterPro IPR000270, PB1_dom IPR034866, PB1_p62 IPR033741, SQSTM_UBA IPR015940, UBA IPR009060, UBA-like_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF16577, UBA_5, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00165, UBA, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50030, UBA, 1 hit PS01357, ZF_ZZ_1, 1 hit PS50135, ZF_ZZ_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASLTVKAYL LGKEEAAREI RRFSFCFSPE PEAEAAAGPG PCERLLSRVA
60 70 80 90 100
VLFPALRPGG FQAHYRDEDG DLVAFSSDEE LTMAMSYVKD DIFRIYIKEK
110 120 130 140 150
KECRREHRPP CAQEARSMVH PNVICDGCNG PVVGTRYKCS VCPDYDLCSV
160 170 180 190 200
CEGKGLHREH SKLIFPNPFG HLSDSFSHSR WLRKLKHGHF GWPGWEMGPP
210 220 230 240 250
GNWSPRPPRA GDGRPCPTAE SASAPSEDPN VNFLKNVGES VAAALSPLGI
260 270 280 290 300
EVDIDVEHGG KRSRLTPTSA ESSSTGTEDK SGTQPSSCSS EVSKPDGAGE
310 320 330 340 350
GPAQSLTEQM KKIALESVGQ PEELMESDNC SGGDDDWTHL SSKEVDPSTG
360 370 380 390 400
ELQSLQMPES EGPSSLDPSQ EGPTGLKEAA LYPHLPPEAD PRLIESLSQM
410 420 430
LSMGFSDEGG WLTRLLQTKN YDIGAALDTI QYSKHPPPL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHB5 | A0A0H2UHB5_RAT | Sequestosome-1 | Sqstm1 | 234 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015843 | 222 – 248 | Missing in isoform 2. CuratedAdd BLAST | 27 | |
Alternative sequenceiVSP_015844 | 222 – 234 | ASAPS…NVNFL → GKAGVCTGFKCHK in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_015845 | 235 – 439 | Missing in isoform 3. 1 PublicationAdd BLAST | 205 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08355 mRNA Translation: CAA69642.1 AF439403 mRNA Translation: AAO15463.1 BC061575 mRNA Translation: AAH61575.1 |
RefSeqi | NP_787037.2, NM_175843.4 [O08623-1] NP_853528.1, NM_181550.1 [O08623-3] XP_006246275.1, XM_006246213.2 [O08623-2] |
Genome annotation databases
Ensembli | ENSRNOT00000059255; ENSRNOP00000056021; ENSRNOG00000003147 [O08623-1] |
GeneIDi | 113894 |
KEGGi | rno:113894 |
UCSCi | RGD:69287, rat [O08623-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08355 mRNA Translation: CAA69642.1 AF439403 mRNA Translation: AAO15463.1 BC061575 mRNA Translation: AAH61575.1 |
RefSeqi | NP_787037.2, NM_175843.4 [O08623-1] NP_853528.1, NM_181550.1 [O08623-3] XP_006246275.1, XM_006246213.2 [O08623-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2K6Q | NMR | - | B | 331-346 | [»] | |
2KKC | NMR | - | A | 3-100 | [»] | |
2KTR | NMR | - | A/B | 3-100 | [»] | |
BMRBi | O08623 | |||||
SMRi | O08623 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250206, 31 interactors |
CORUMi | O08623 |
IntActi | O08623, 5 interactors |
MINTi | O08623 |
STRINGi | 10116.ENSRNOP00000056021 |
PTM databases
iPTMneti | O08623 |
PhosphoSitePlusi | O08623 |
Proteomic databases
jPOSTi | O08623 |
PaxDbi | O08623 |
PRIDEi | O08623 |
Genome annotation databases
Ensembli | ENSRNOT00000059255; ENSRNOP00000056021; ENSRNOG00000003147 [O08623-1] |
GeneIDi | 113894 |
KEGGi | rno:113894 |
UCSCi | RGD:69287, rat [O08623-1] |
Organism-specific databases
CTDi | 8878 |
RGDi | 69287, Sqstm1 |
Phylogenomic databases
eggNOGi | KOG4582, Eukaryota |
GeneTreei | ENSGT00390000002781 |
HOGENOMi | CLU_038011_1_0_1 |
InParanoidi | O08623 |
OMAi | WHPLQWF |
OrthoDBi | 1275680at2759 |
PhylomeDBi | O08623 |
Enzyme and pathway databases
Reactomei | R-RNO-205043, NRIF signals cell death from the nucleus R-RNO-209543, p75NTR recruits signalling complexes R-RNO-209560, NF-kB is activated and signals survival R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-9020702, Interleukin-1 signaling R-RNO-9664873, Pexophagy |
Miscellaneous databases
EvolutionaryTracei | O08623 |
PROi | PR:O08623 |
Gene expression databases
Bgeei | ENSRNOG00000003147, Expressed in skeletal muscle tissue and 21 other tissues |
ExpressionAtlasi | O08623, baseline and differential |
Genevisiblei | O08623, RN |
Family and domain databases
CDDi | cd06402, PB1_p62, 1 hit cd14320, UBA_SQSTM, 1 hit |
Gene3Di | 3.30.60.90, 1 hit |
IDEALi | IID50133 |
InterProi | View protein in InterPro IPR000270, PB1_dom IPR034866, PB1_p62 IPR033741, SQSTM_UBA IPR015940, UBA IPR009060, UBA-like_sf IPR000433, Znf_ZZ IPR043145, Znf_ZZ_sf |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF16577, UBA_5, 1 hit PF00569, ZZ, 1 hit |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00165, UBA, 1 hit SM00291, ZnF_ZZ, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50030, UBA, 1 hit PS01357, ZF_ZZ_1, 1 hit PS50135, ZF_ZZ_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SQSTM_RAT | |
Accessioni | O08623Primary (citable) accession number: O08623 Secondary accession number(s): Q8CH59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references