UniProtKB - O08609 (MLX_MOUSE)
Max-like protein X
Mlx
Functioni
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
3 PublicationsGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- nucleocytoplasmic transport Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-163765, ChREBP activates metabolic gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Max-like protein XAlternative name(s): Max-like bHLHZip protein Protein BigMax Transcription factor-like protein 4 |
Gene namesi | Name:Mlx Synonyms:Tcfl4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108398, Mlx |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Found predominantly in the cytoplasm.By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Found predominantly in the cytoplasm.By similarity
Nucleus
- Nucleus By similarity
Note: Found predominantly in the nucleus.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear membrane Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127280 | 1 – 298 | Max-like protein XAdd BLAST | 298 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 7 | PhosphoserineBy similarity | 1 | |
Modified residuei | 45 | PhosphoserineCombined sources | 1 | |
Modified residuei | 48 | PhosphoserineCombined sources | 1 | |
Modified residuei | 74 | PhosphoserineBy similarity | 1 | |
Modified residuei | 77 | PhosphoserineBy similarity | 1 | |
Modified residuei | 98 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O08609 |
MaxQBi | O08609 |
PaxDbi | O08609 |
PRIDEi | O08609 |
ProteomicsDBi | 290261 [O08609-1] 290262 [O08609-2] 290263 [O08609-3] |
PTM databases
iPTMneti | O08609 |
PhosphoSitePlusi | O08609 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.
GO - Molecular functioni
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204019, 1 interactor |
CORUMi | O08609 |
STRINGi | 10090.ENSMUSP00000017945 |
Miscellaneous databases
RNActi | O08609, protein |
Structurei
3D structure databases
AlphaFoldDBi | O08609 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 129 – 187 | bHLHPROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 63 | DisorderedSequence analysisAdd BLAST | 63 | |
Regioni | 98 – 119 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 194 – 214 | Leucine-zipperAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 98 – 112 | Polar residuesSequence analysisAdd BLAST | 15 |
Phylogenomic databases
eggNOGi | KOG1319, Eukaryota |
InParanoidi | O08609 |
OrthoDBi | 1234389at2759 |
PhylomeDBi | O08609 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032647, Mlx |
PANTHERi | PTHR15741:SF25, PTHR15741:SF25, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTEPGASPED PWVKASFADA HAGEGRAGRA RARRGSGRRG APQLSPESPL
60 70 80 90 100
LSGARGCRED SSHPACAKVE YAYSDNSLDP GLFVESTHKG SVVSRANSIG
110 120 130 140 150
STSASSVPNT DDEDSDYQQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD
160 170 180 190 200
DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST
210 220 230 240 250
LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF
260 270 280 290
QSFNASISVA SFQELSACVF SWIEEHCEPQ TLREIVIGVL HQLKNQLY
The sequence of this isoform differs from the canonical sequence as follows:
15-68: Missing.
81-110: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
15-68: Missing.
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2BFB0 | A2BFB0_MOUSE | Max-like protein X | Mlx | 298 | Annotation score: | ||
A2BFB1 | A2BFB1_MOUSE | Max-like protein X | Mlx | 244 | Annotation score: | ||
A2BFB2 | A2BFB2_MOUSE | Max-like protein X | Mlx | 214 | Annotation score: | ||
A2BFA9 | A2BFA9_MOUSE | Max-like protein X | Mlx | 127 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002139 | 15 – 68 | Missing in isoform Alpha and isoform Beta. CuratedAdd BLAST | 54 | |
Alternative sequenceiVSP_002140 | 81 – 110 | Missing in isoform Alpha. CuratedAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43607 mRNA Translation: AAB51368.1 U43548 mRNA Translation: AAB51367.1 AF213670 mRNA Translation: AAG40149.1 AF213671 mRNA Translation: AAG40150.1 AF213672 mRNA Translation: AAG40151.1 |
CCDSi | CCDS25447.1 [O08609-1] CCDS48935.1 [O08609-3] CCDS48936.1 [O08609-2] |
RefSeqi | NP_001152856.1, NM_001159384.1 NP_001152857.1, NM_001159385.1 NP_035680.3, NM_011550.3 |
Genome annotation databases
GeneIDi | 21428 |
KEGGi | mmu:21428 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43607 mRNA Translation: AAB51368.1 U43548 mRNA Translation: AAB51367.1 AF213670 mRNA Translation: AAG40149.1 AF213671 mRNA Translation: AAG40150.1 AF213672 mRNA Translation: AAG40151.1 |
CCDSi | CCDS25447.1 [O08609-1] CCDS48935.1 [O08609-3] CCDS48936.1 [O08609-2] |
RefSeqi | NP_001152856.1, NM_001159384.1 NP_001152857.1, NM_001159385.1 NP_035680.3, NM_011550.3 |
3D structure databases
AlphaFoldDBi | O08609 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204019, 1 interactor |
CORUMi | O08609 |
STRINGi | 10090.ENSMUSP00000017945 |
PTM databases
iPTMneti | O08609 |
PhosphoSitePlusi | O08609 |
Proteomic databases
EPDi | O08609 |
MaxQBi | O08609 |
PaxDbi | O08609 |
PRIDEi | O08609 |
ProteomicsDBi | 290261 [O08609-1] 290262 [O08609-2] 290263 [O08609-3] |
Protocols and materials databases
DNASUi | 21428 |
Genome annotation databases
GeneIDi | 21428 |
KEGGi | mmu:21428 |
Organism-specific databases
CTDi | 6945 |
MGIi | MGI:108398, Mlx |
Phylogenomic databases
eggNOGi | KOG1319, Eukaryota |
InParanoidi | O08609 |
OrthoDBi | 1234389at2759 |
PhylomeDBi | O08609 |
Enzyme and pathway databases
Reactomei | R-MMU-163765, ChREBP activates metabolic gene expression |
Miscellaneous databases
BioGRID-ORCSi | 21428, 3 hits in 75 CRISPR screens |
ChiTaRSi | Mlx, mouse |
PROi | PR:O08609 |
RNActi | O08609, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032647, Mlx |
PANTHERi | PTHR15741:SF25, PTHR15741:SF25, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MLX_MOUSE | |
Accessioni | O08609Primary (citable) accession number: O08609 Secondary accession number(s): Q9EQJ7, Q9EQJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | July 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot