UniProtKB - O08609 (MLX_MOUSE)

BLAST

>sp|O08609|MLX_MOUSE Max-like protein X OS=Mus musculus OX=10090 GN=Mlx PE=1 SV=1
MTEPGASPEDPWVKASFADAHAGEGRAGRARARRGSGRRGAPQLSPESPLLSGARGCRED
SSHPACAKVEYAYSDNSLDPGLFVESTHKGSVVSRANSIGSTSASSVPNTDDEDSDYQQE
AYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKT
IDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDNPHEGEDQVSDQVKFN
VFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQTLREIVIGVLHQLKNQLY

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History
Entry version 151 (07 Nov 2018)
Sequence version 1 (01 Jul 1997)
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Protein

Max-like protein X

Gene

Mlx

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.3 Publications

GO - Molecular functionⁱ

DNA binding Source: MGI
DNA-binding transcription factor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of transcription, DNA-templated Source: MGI
negative regulation of transcription by RNA polymerase II Source: MGI
nucleocytoplasmic transport
Source: MGI
Inferred from direct assayⁱ
- 12446771
positive regulation of transcription by RNA polymerase II Source: MGI
regulation of transcription by RNA polymerase II
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Max-like protein X Alternative name(s): Max-like bHLHZip protein Protein BigMax Transcription factor-like protein 4
Gene namesⁱ	Name:Mlx Synonyms:Tcfl4
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:108398 Mlx

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127280	1 – 298	Max-like protein XAdd BLAST		298

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	7	PhosphoserineBy similarity	1
Modified residueⁱ	45	PhosphoserineCombined sources	1
Modified residueⁱ	48	PhosphoserineCombined sources	1
Modified residueⁱ	74	PhosphoserineBy similarity	1
Modified residueⁱ	77	PhosphoserineBy similarity	1
Modified residueⁱ	98	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O08609
PaxDbⁱ	O08609
PRIDEⁱ	O08609

PTM databases

iPTMnetⁱ	O08609
PhosphoSitePlusⁱ	O08609

Expressionⁱ

Tissue specificityⁱ

Expressed in all tissues examined: stomach, duodenum, jejunum, ileum, colon, liver, pancreas, salivary gland, kidney, spleen, lung, heart, skeletal muscle, brain, ovary and testis.1 Publication

Developmental stageⁱ

Expression peaks between dpc 9.5 to 11.5, then decreases during later stages of organogenesis.1 Publication

Gene expression databases

CleanExⁱ	MM_MLX

CleanExⁱ

MM_MLX

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAD1, MAD4, MNT, WBSCR14 and MLXIP. Can also bind DNA as a homodimer.

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	204019, 1 interactor
CORUMⁱ	O08609
STRINGⁱ	10090.ENSMUSP00000017945

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O08609
SMRⁱ	O08609
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	129 – 187	bHLHPROSITE-ProRule annotationAdd BLAST		59

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	194 – 214	Leucine-zipperAdd BLAST		21

Phylogenomic databases

eggNOGⁱ	KOG1319 Eukaryota ENOG410ZWIP LUCA
HOGENOMⁱ	HOG000232118
HOVERGENⁱ	HBG019061
InParanoidⁱ	O08609
KEGG Orthology (KO) More...KOⁱ	K09113
PhylomeDBⁱ	O08609

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032647 Mlx
PANTHERⁱ	PTHR15741:SF25 PTHR15741:SF25, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform Gamma (identifier: O08609-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKASFADA HAGEGRAGRA RARRGSGRRG APQLSPESPL 
        60         70         80         90        100
LSGARGCRED SSHPACAKVE YAYSDNSLDP GLFVESTHKG SVVSRANSIG 
       110        120        130        140        150
STSASSVPNT DDEDSDYQQE AYKESYKDRR RRAHTQAEQK RRDAIKRGYD 
       160        170        180        190        200
DLQTIVPTCQ QQDFSIGSQK LSKAIVLQKT IDYIQFLHKE KKKQEEEVST 
       210        220        230        240        250
LRKDVTALKI MKVNYEQIVK AHQDNPHEGE DQVSDQVKFN VFQGIMDSLF 
       260        270        280        290 
QSFNASISVA SFQELSACVF SWIEEHCEPQ TLREIVIGVL HQLKNQLY

Length:298

Mass (Da):33,339

Last modified:July 1, 1997 - v1

Checksum:ⁱA494D5A86292B71C

Isoform Alpha (identifier: O08609-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
15-68: Missing.
81-110: Missing.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKVEYAYS DNSLDPDDED SDYQQEAYKE SYKDRRRRAH 
        60         70         80         90        100
TQAEQKRRDA IKRGYDDLQT IVPTCQQQDF SIGSQKLSKA IVLQKTIDYI 
       110        120        130        140        150
QFLHKEKKKQ EEEVSTLRKD VTALKIMKVN YEQIVKAHQD NPHEGEDQVS 
       160        170        180        190        200
DQVKFNVFQG IMDSLFQSFN ASISVASFQE LSACVFSWIE EHCEPQTLRE 
       210 
IVIGVLHQLK NQLY

Show »

Length:214

Mass (Da):24,897

Checksum:ⁱ7C7690DFF2CEF921

Isoform Beta (identifier: O08609-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
15-68: Missing.

« Hide

        10         20         30         40         50
MTEPGASPED PWVKVEYAYS DNSLDPGLFV ESTHKGSVVS RANSIGSTSA 
        60         70         80         90        100
SSVPNTDDED SDYQQEAYKE SYKDRRRRAH TQAEQKRRDA IKRGYDDLQT 
       110        120        130        140        150
IVPTCQQQDF SIGSQKLSKA IVLQKTIDYI QFLHKEKKKQ EEEVSTLRKD 
       160        170        180        190        200
VTALKIMKVN YEQIVKAHQD NPHEGEDQVS DQVKFNVFQG IMDSLFQSFN 
       210        220        230        240 
ASISVASFQE LSACVFSWIE EHCEPQTLRE IVIGVLHQLK NQLY

Show »

Length:244

Mass (Da):27,856

Checksum:ⁱ1D867FCAA26764B0

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A2BFB0	A2BFB0_MOUSE	MAX-like protein X, isoform CRA_b MAX-like protein X, isoform CRA_b (Max-like protein X)	Mlx mCG_20239	298	Annotation score:
A2BFB1	A2BFB1_MOUSE	Max-like protein X Max-like protein X	Mlx	244	Annotation score:
A2BFB2	A2BFB2_MOUSE	MAX-like protein X, isoform CRA_a MAX-like protein X, isoform CRA_a (Max-like protein X)	Mlx mCG_20239	214	Annotation score:
A2BFA9	A2BFA9_MOUSE	Max-like protein X Max-like protein X	Mlx	127	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_002139	15 – 68	Missing in isoform Alpha and isoform Beta. CuratedAdd BLAST		54
Alternative sequenceⁱVSP_002140	81 – 110	Missing in isoform Alpha. CuratedAdd BLAST		30

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U43607 mRNA Translation: AAB51368.1 U43548 mRNA Translation: AAB51367.1 AF213670 mRNA Translation: AAG40149.1 AF213671 mRNA Translation: AAG40150.1 AF213672 mRNA Translation: AAG40151.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25447.1 [O08609-1] CCDS48935.1 [O08609-3] CCDS48936.1 [O08609-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001152856.1, NM_001159384.1 NP_001152857.1, NM_001159385.1 NP_035680.3, NM_011550.3
UniGeneⁱ	Mm.628

Genome annotation databases

GeneIDⁱ	21428
KEGGⁱ	mmu:21428

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O08609	Max-like protein X		HUMAN		298	UniRef90_Q9UH92
MLX isoform 1		PANTR		298
Uncharacterized protein		PANPA		298
Uncharacterized protein		PIG		298
MLX, MAX dimerization protein		MACMU		298
+56
O08609-2	max-like protein X isoform X4		Enhydra lutris kenyoni		259	UniRef90_O08609-2
max-like protein X isoform X2		ODORO		224
MAX-like protein X, isoform CRA_a		MOUSE		214
max-like protein X isoform X3		MESAU		214
max-like protein X-like isoform X4		BALAS		214
+15
O08609-3	Max-like protein X isoform beta		CALJA		244	UniRef90_O08609-3
Uncharacterized protein		Cebus capucinus imitator		244
Uncharacterized protein		AOTNA		244
Max-like protein X isoform beta		MACMU		244
Uncharacterized protein		AILME		244
+39

Protein	Similar proteins		Species	Score	Length	Source
O08609	Max-like protein X		HUMAN		298	UniRef50_Q9UH92
MLX isoform 1		PANTR		298
Uncharacterized protein		PANPA		298
Uncharacterized protein		CALJA		298
MAX-like protein X		RAT		298
+117
O08609-2	Isoform Alpha of Max-like protein X		HUMAN		214	UniRef50_Q9UH92-2
MLX, MAX dimerization protein		GORGO		255
MAX-like protein X		PANTR		214
Uncharacterized protein		MACMU		214
Uncharacterized protein		COLAP		214
+461
O08609-3	Isoform Alpha of Max-like protein X		HUMAN		214	UniRef50_Q9UH92-2
MLX, MAX dimerization protein		GORGO		255
MAX-like protein X		PANTR		214
Uncharacterized protein		MACMU		214
Uncharacterized protein		COLAP		214
+461

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U43607 mRNA Translation: AAB51368.1 U43548 mRNA Translation: AAB51367.1 AF213670 mRNA Translation: AAG40149.1 AF213671 mRNA Translation: AAG40150.1 AF213672 mRNA Translation: AAG40151.1
CCDSⁱ	CCDS25447.1 [O08609-1] CCDS48935.1 [O08609-3] CCDS48936.1 [O08609-2]
RefSeqⁱ	NP_001152856.1, NM_001159384.1 NP_001152857.1, NM_001159385.1 NP_035680.3, NM_011550.3
UniGeneⁱ	Mm.628

3D structure databases

ProteinModelPortalⁱ	O08609
SMRⁱ	O08609
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	204019, 1 interactor
CORUMⁱ	O08609
STRINGⁱ	10090.ENSMUSP00000017945

PTM databases

iPTMnetⁱ	O08609
PhosphoSitePlusⁱ	O08609

Proteomic databases

MaxQBⁱ	O08609
PaxDbⁱ	O08609
PRIDEⁱ	O08609

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	21428
KEGGⁱ	mmu:21428

Organism-specific databases

CTDⁱ	6945
MGIⁱ	MGI:108398 Mlx

Phylogenomic databases

eggNOGⁱ	KOG1319 Eukaryota ENOG410ZWIP LUCA
HOGENOMⁱ	HOG000232118
HOVERGENⁱ	HBG019061
InParanoidⁱ	O08609
KOⁱ	K09113
PhylomeDBⁱ	O08609

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O08609
SOURCEⁱ	Search...

Gene expression databases

CleanExⁱ	MM_MLX

CleanExⁱ

MM_MLX

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf IPR032647 Mlx
PANTHERⁱ	PTHR15741:SF25 PTHR15741:SF25, 1 hit
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MLX_MOUSE
Accessionⁱ	O08609Primary (citable) accession number: O08609 Secondary accession number(s): Q9EQJ7, Q9EQJ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
	Last sequence update:	July 1, 1997
	Last modified:	November 7, 2018
	This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O08609 (MLX_MOUSE)

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Max-like protein X

Mlx

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Other locations

Nucleus

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ