UniProtKB - O08575 (EYA2_MOUSE)
Protein
Eyes absent homolog 2
Gene
Eya2
Organism
Mus musculus (Mouse)
Status
Functioni
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:10490620, PubMed:17098221). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (By similarity). Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (By similarity).By similarity2 Publications
Catalytic activityi
- EC:3.1.3.481 Publication
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 268 | NucleophileBy similarity | 1 | |
Metal bindingi | 268 | MagnesiumBy similarity | 1 | |
Active sitei | 270 | Proton donorBy similarity | 1 | |
Metal bindingi | 270 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 496 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB
- protein tyrosine phosphatase activity Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- anatomical structure development Source: GO_Central
- cell differentiation Source: GO_Central
- DNA repair Source: UniProtKB-KW
- extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
- histone dephosphorylation Source: UniProtKB
- mitochondrial outer membrane permeabilization Source: MGI
- multicellular organism development Source: UniProtKB-KW
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
- positive regulation of DNA repair Source: GO_Central
- protein dephosphorylation Source: MGI
- striated muscle tissue development Source: MGI
Keywordsi
Molecular function | Activator, Chromatin regulator, Developmental protein, Hydrolase, Protein phosphatase |
Biological process | DNA damage, DNA repair, Transcription, Transcription regulation |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Eya2 Synonyms:Eab1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109341, Eya2 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: GOC
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
No visible phenotype. In contrast, mice lacking both Eya1 and Eya2 display complete embryonic lethality, due to severe defects in muscle development, including the absence of the diaphragm, the absence of ventral hypaxial muscles of the trunk and muscles in forelimbs and hindlimbs, similar to the phenotype of mice lacking both Six1 and Six4. While Six1 is normally expressed in these mice, it does not activate transcription from cognate promoter elements, and does not activate transcription of Pax3.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218647 | 1 – 532 | Eyes absent homolog 2Add BLAST | 532 |
Proteomic databases
PaxDbi | O08575 |
PeptideAtlasi | O08575 |
PRIDEi | O08575 |
PTM databases
iPTMneti | O08575 |
PhosphoSitePlusi | O08575 |
Expressioni
Tissue specificityi
Extensively expressed in cranial placodes, branchial arches, CNS and developing eye and nose. Low expression in lung with little or no expression in skin, liver, intestine and kidney. Predominantly expressed in the upper one-third of the oxyntic mucosa and in most regions of the pyloric mucosa.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000017897, Expressed in undifferentiated genital tubercle and 137 other tissues |
Genevisiblei | O08575, MM |
Interactioni
Subunit structurei
Probably interacts with SIX2, SIX4 and SIX5.
Interacts with DACH2.
Interacts with GNAZ and GNAI2; this retains EYA2 in the cytoplasm and prevents its translocation into the nucleus and transcriptional activity (By similarity).
Interacts with SIX1.
Interacts with CAPN8.
By similarity3 PublicationsBinary interactionsi
O08575
With | #Exp. | IntAct |
---|---|---|
Sharpin [Q91WA6] | 2 | EBI-986503,EBI-646097 |
GO - Molecular functioni
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199560, 1 interactor |
IntActi | O08575, 3 interactors |
STRINGi | 10090.ENSMUSP00000066244 |
Miscellaneous databases
RNActi | O08575, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3107, Eukaryota |
GeneTreei | ENSGT00950000182978 |
HOGENOMi | CLU_021184_2_0_1 |
InParanoidi | O08575 |
OMAi | YNSPYMS |
TreeFami | TF319337 |
Family and domain databases
CDDi | cd02601, HAD_Eya, 1 hit |
Gene3Di | 3.40.50.12350, 1 hit |
InterProi | View protein in InterPro IPR028473, EYA2 IPR006545, EYA_dom IPR042577, EYA_dom_metazoan IPR038102, EYA_dom_sf IPR028472, EYA_fam IPR036412, HAD-like_sf |
PANTHERi | PTHR10190, PTHR10190, 1 hit PTHR10190:SF7, PTHR10190:SF7, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01658, EYA-cons_domain, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O08575-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLEVVTSPSL ATSSDWSEHG AAVGTLSDRE GIAKSAALSV PQLFVKSHPR
60 70 80 90 100
VPPGQSSTAM AAYGQTQYST GIQQAPPYTA YPTPAQAYGI PPYSIKTEDS
110 120 130 140 150
LNHSPSQSGF LSYGPSFSTA PAGQSPYTYP VHSTAGLYQG ANGLTNTAGF
160 170 180 190 200
GSVHQDYPSY PSFSQNQYPQ YFSPSYNPPY VPASSLCSSP LSTSTYVLQE
210 220 230 240 250
APHNVPSQSS ESLAGDYNTH NGPSTPAKEG DTERPHRASD GKLRGRSKRN
260 270 280 290 300
SDPSPAGDNE IERVFVWDLD ETIIIFHSLL TGTFASRYGK DTTTSVRIGL
310 320 330 340 350
MMEEMIFNLA DTHLFFNDLE DCDQIHVDDV SSDDNGQDLS TYNFSTDGFH
360 370 380 390 400
STAPGASLCL GTGVHGGVDW MRKLAFRYRR VKEMYNTYRN NVGGLIGAPK
410 420 430 440 450
RETWLQLRAE LEALTDLWLT HSLKALNLIN SRPNCVNVLV TTTQLIPALA
460 470 480 490 500
KVLLYGLGSV FPIENIYSAT KTGKESCFER IMQRFGRKAV YIVIGDGVEE
510 520 530
EQGAKKHNMP FWRISCHADL EALRHALELE YL
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001492 | 114 – 142 | Missing in isoform 2. CuratedAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71208 mRNA Translation: AAB51121.1 U61111 mRNA Translation: AAB48018.1 Sequence problems. AL591712 Genomic DNA No translation available. BC003755 mRNA Translation: AAH03755.1 U81603 mRNA Translation: AAB42067.1 |
CCDSi | CCDS17084.1 [O08575-1] |
RefSeqi | NP_001258891.1, NM_001271962.1 [O08575-1] NP_001258892.1, NM_001271963.1 [O08575-1] NP_034295.1, NM_010165.3 [O08575-1] |
Genome annotation databases
Ensembli | ENSMUST00000063433; ENSMUSP00000066244; ENSMUSG00000017897 [O08575-1] ENSMUST00000088132; ENSMUSP00000085455; ENSMUSG00000017897 [O08575-1] |
GeneIDi | 14049 |
KEGGi | mmu:14049 |
UCSCi | uc008nxw.2, mouse [O08575-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71208 mRNA Translation: AAB51121.1 U61111 mRNA Translation: AAB48018.1 Sequence problems. AL591712 Genomic DNA No translation available. BC003755 mRNA Translation: AAH03755.1 U81603 mRNA Translation: AAB42067.1 |
CCDSi | CCDS17084.1 [O08575-1] |
RefSeqi | NP_001258891.1, NM_001271962.1 [O08575-1] NP_001258892.1, NM_001271963.1 [O08575-1] NP_034295.1, NM_010165.3 [O08575-1] |
3D structure databases
SMRi | O08575 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199560, 1 interactor |
IntActi | O08575, 3 interactors |
STRINGi | 10090.ENSMUSP00000066244 |
PTM databases
iPTMneti | O08575 |
PhosphoSitePlusi | O08575 |
Proteomic databases
PaxDbi | O08575 |
PeptideAtlasi | O08575 |
PRIDEi | O08575 |
Protocols and materials databases
Antibodypediai | 13258, 256 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000063433; ENSMUSP00000066244; ENSMUSG00000017897 [O08575-1] ENSMUST00000088132; ENSMUSP00000085455; ENSMUSG00000017897 [O08575-1] |
GeneIDi | 14049 |
KEGGi | mmu:14049 |
UCSCi | uc008nxw.2, mouse [O08575-1] |
Organism-specific databases
CTDi | 2139 |
MGIi | MGI:109341, Eya2 |
Phylogenomic databases
eggNOGi | KOG3107, Eukaryota |
GeneTreei | ENSGT00950000182978 |
HOGENOMi | CLU_021184_2_0_1 |
InParanoidi | O08575 |
OMAi | YNSPYMS |
TreeFami | TF319337 |
Enzyme and pathway databases
Reactomei | R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Miscellaneous databases
BioGRID-ORCSi | 14049, 0 hits in 17 CRISPR screens |
ChiTaRSi | Eya2, mouse |
PROi | PR:O08575 |
RNActi | O08575, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017897, Expressed in undifferentiated genital tubercle and 137 other tissues |
Genevisiblei | O08575, MM |
Family and domain databases
CDDi | cd02601, HAD_Eya, 1 hit |
Gene3Di | 3.40.50.12350, 1 hit |
InterProi | View protein in InterPro IPR028473, EYA2 IPR006545, EYA_dom IPR042577, EYA_dom_metazoan IPR038102, EYA_dom_sf IPR028472, EYA_fam IPR036412, HAD-like_sf |
PANTHERi | PTHR10190, PTHR10190, 1 hit PTHR10190:SF7, PTHR10190:SF7, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01658, EYA-cons_domain, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EYA2_MOUSE | |
Accessioni | O08575Primary (citable) accession number: O08575 Secondary accession number(s): A2A5S4, P97925 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families