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Protein

Eyes absent homolog 2

Gene

Eya2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:10490620, PubMed:17098221). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (By similarity). Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (By similarity).By similarity2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei268NucleophileBy similarity1
Metal bindingi268MagnesiumBy similarity1
Active sitei270Proton donorBy similarity1
Metal bindingi270Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi496MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Developmental protein, Hydrolase, Protein phosphatase
Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Names & Taxonomyi

Protein namesi
Recommended name:
Eyes absent homolog 2 (EC:3.1.3.481 Publication)
Gene namesi
Name:Eya2
Synonyms:Eab1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109341 Eya2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. In contrast, mice lacking both Eya1 and Eya2 display complete embryonic lethality, due to severe defects in muscle development, including the absence of the diaphragm, the absence of ventral hypaxial muscles of the trunk and muscles in forelimbs and hindlimbs, similar to the phenotype of mice lacking both Six1 and Six4. While Six1 is normally expressed in these mice, it does not activate transcription from cognate promoter elements, and does not activate transcription of Pax3.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186471 – 532Eyes absent homolog 2Add BLAST532

Proteomic databases

PaxDbiO08575
PeptideAtlasiO08575
PRIDEiO08575

PTM databases

iPTMnetiO08575
PhosphoSitePlusiO08575

Expressioni

Tissue specificityi

Extensively expressed in cranial placodes, branchial arches, CNS and developing eye and nose. Low expression in lung with little or no expression in skin, liver, intestine and kidney. Predominantly expressed in the upper one-third of the oxyntic mucosa and in most regions of the pyloric mucosa.1 Publication

Gene expression databases

BgeeiENSMUSG00000017897 Expressed in 110 organ(s), highest expression level in stomach
CleanExiMM_EYA2
GenevisibleiO08575 MM

Interactioni

Subunit structurei

Probably interacts with SIX2, SIX4 and SIX5. Interacts with DACH2. Interacts with GNAZ and GNAI2; this retains EYA2 in the cytoplasm and prevents its translocation into the nucleus and transcriptional activity (By similarity). Interacts with SIX1. Interacts with CAPN8.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SharpinQ91WA62EBI-986503,EBI-646097

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199560, 1 interactor
IntActiO08575, 3 interactors
STRINGi10090.ENSMUSP00000066244

Structurei

3D structure databases

ProteinModelPortaliO08575
SMRiO08575
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3107 Eukaryota
ENOG410XT12 LUCA
GeneTreeiENSGT00390000008860
HOVERGENiHBG002447
InParanoidiO08575
KOiK17620
OMAiSADGFHS
OrthoDBiEOG091G04YD
TreeFamiTF319337

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR028473 EYA2
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
IPR036412 HAD-like_sf
PANTHERiPTHR10190 PTHR10190, 1 hit
PTHR10190:SF7 PTHR10190:SF7, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01658 EYA-cons_domain, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O08575-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLEVVTSPSL ATSSDWSEHG AAVGTLSDRE GIAKSAALSV PQLFVKSHPR
60 70 80 90 100
VPPGQSSTAM AAYGQTQYST GIQQAPPYTA YPTPAQAYGI PPYSIKTEDS
110 120 130 140 150
LNHSPSQSGF LSYGPSFSTA PAGQSPYTYP VHSTAGLYQG ANGLTNTAGF
160 170 180 190 200
GSVHQDYPSY PSFSQNQYPQ YFSPSYNPPY VPASSLCSSP LSTSTYVLQE
210 220 230 240 250
APHNVPSQSS ESLAGDYNTH NGPSTPAKEG DTERPHRASD GKLRGRSKRN
260 270 280 290 300
SDPSPAGDNE IERVFVWDLD ETIIIFHSLL TGTFASRYGK DTTTSVRIGL
310 320 330 340 350
MMEEMIFNLA DTHLFFNDLE DCDQIHVDDV SSDDNGQDLS TYNFSTDGFH
360 370 380 390 400
STAPGASLCL GTGVHGGVDW MRKLAFRYRR VKEMYNTYRN NVGGLIGAPK
410 420 430 440 450
RETWLQLRAE LEALTDLWLT HSLKALNLIN SRPNCVNVLV TTTQLIPALA
460 470 480 490 500
KVLLYGLGSV FPIENIYSAT KTGKESCFER IMQRFGRKAV YIVIGDGVEE
510 520 530
EQGAKKHNMP FWRISCHADL EALRHALELE YL
Length:532
Mass (Da):58,246
Last modified:July 1, 1997 - v1
Checksum:i9A29BC6BB77DF880
GO
Isoform 2 (identifier: O08575-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-142: Missing.

Show »
Length:503
Mass (Da):55,337
Checksum:iF12F8FD4B0291F16
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001492114 – 142Missing in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71208 mRNA Translation: AAB51121.1
U61111 mRNA Translation: AAB48018.1 Sequence problems.
AL591712 Genomic DNA No translation available.
BC003755 mRNA Translation: AAH03755.1
U81603 mRNA Translation: AAB42067.1
CCDSiCCDS17084.1 [O08575-1]
RefSeqiNP_001258891.1, NM_001271962.1 [O08575-1]
NP_001258892.1, NM_001271963.1 [O08575-1]
NP_034295.1, NM_010165.3 [O08575-1]
UniGeneiMm.282719

Genome annotation databases

EnsembliENSMUST00000063433; ENSMUSP00000066244; ENSMUSG00000017897 [O08575-1]
ENSMUST00000088132; ENSMUSP00000085455; ENSMUSG00000017897 [O08575-1]
GeneIDi14049
KEGGimmu:14049
UCSCiuc008nxw.2 mouse [O08575-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71208 mRNA Translation: AAB51121.1
U61111 mRNA Translation: AAB48018.1 Sequence problems.
AL591712 Genomic DNA No translation available.
BC003755 mRNA Translation: AAH03755.1
U81603 mRNA Translation: AAB42067.1
CCDSiCCDS17084.1 [O08575-1]
RefSeqiNP_001258891.1, NM_001271962.1 [O08575-1]
NP_001258892.1, NM_001271963.1 [O08575-1]
NP_034295.1, NM_010165.3 [O08575-1]
UniGeneiMm.282719

3D structure databases

ProteinModelPortaliO08575
SMRiO08575
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199560, 1 interactor
IntActiO08575, 3 interactors
STRINGi10090.ENSMUSP00000066244

PTM databases

iPTMnetiO08575
PhosphoSitePlusiO08575

Proteomic databases

PaxDbiO08575
PeptideAtlasiO08575
PRIDEiO08575

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063433; ENSMUSP00000066244; ENSMUSG00000017897 [O08575-1]
ENSMUST00000088132; ENSMUSP00000085455; ENSMUSG00000017897 [O08575-1]
GeneIDi14049
KEGGimmu:14049
UCSCiuc008nxw.2 mouse [O08575-1]

Organism-specific databases

CTDi2139
MGIiMGI:109341 Eya2

Phylogenomic databases

eggNOGiKOG3107 Eukaryota
ENOG410XT12 LUCA
GeneTreeiENSGT00390000008860
HOVERGENiHBG002447
InParanoidiO08575
KOiK17620
OMAiSADGFHS
OrthoDBiEOG091G04YD
TreeFamiTF319337

Enzyme and pathway databases

ReactomeiR-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks

Miscellaneous databases

PROiPR:O08575
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017897 Expressed in 110 organ(s), highest expression level in stomach
CleanExiMM_EYA2
GenevisibleiO08575 MM

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR028473 EYA2
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
IPR036412 HAD-like_sf
PANTHERiPTHR10190 PTHR10190, 1 hit
PTHR10190:SF7 PTHR10190:SF7, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01658 EYA-cons_domain, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEYA2_MOUSE
AccessioniPrimary (citable) accession number: O08575
Secondary accession number(s): A2A5S4, P97925
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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