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Protein

Mesoderm posterior protein 2

Gene

Mesp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.10 Publications

GO - Molecular functioni

GO - Biological processi

  • heart morphogenesis Source: GO_Central
  • mesodermal cell migration Source: MGI
  • Notch signaling pathway Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • signal transduction involved in regulation of gene expression Source: MGI
  • somite rostral/caudal axis specification Source: MGI
  • somitogenesis Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processNotch signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mesoderm posterior protein 2
Gene namesi
Name:Mesp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1096325 Mesp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die shortly after birth and show caudal truncation and severe skeletal malformations. Lack of segmentation and impaired segment polarity of the paraxial mesoderm are the primary defects. Mutants show altered expression of MEOX1, Pax1, and DLL1 and lack of expression of Notch1, Notch2, and FGFR1. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major defect is the apparent lack of any mesodermal layer between endoderm and ectoderm and a defect in the migratory activity of mesodermal cells.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi70 – 73RRTR → AATA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 145-A--A-150. 1 Publication4
Mutagenesisi145 – 150RRRRRR → AAAAAA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 70-A--A-73. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002963021 – 370Mesoderm posterior protein 2Add BLAST370

Post-translational modificationi

Degraded by the proteasome.
Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO08574

PTM databases

iPTMnetiO08574
PhosphoSitePlusiO08574

Expressioni

Developmental stagei

First transiently detected in the nascent mesoderm at the onset of gastrulation. A second site of expression is detected at 8 dpc in the rostral region of the presomitic mesoderm inmediately before segmentation. Down-regulated immediately after the formation of the segmented somites before 13.5 dpc. Initially expressed throughout the length of one somite, and then quickly repressed in the presumptive caudal region. Mesp2 is involved in the rapid down-regulation of its own expression in the presumptive caudal half of the somite.4 Publications

Inductioni

Down-regulated in NOTCH1, DLL1, RBPJ and FOXC1/FOXC2 mutant mices. Expression in the anterior presomitic mesoderm is periodic, dissipating once the segmental border is established. Negatively regulated by RIPPLY2. Positively regulated by TBX6 and Notch signaling.5 Publications

Gene expression databases

CleanExiMM_MESP2

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliO08574
SMRiO08574
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 133bHLHPROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni326 – 330May contain a degradation domain5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi145 – 150Poly-Arg6

Phylogenomic databases

HOVERGENiHBG096371
InParanoidiO08574
KOiK09076
PhylomeDBiO08574

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O08574-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQSSPPQSL QGLVPLGLLP GLGLGSAIGL HVSGLVLRFV RFLPFYATRR
60 70 80 90 100
PSQPAGPARS TRTTQATAPR RTRPAPAGGQ RQSASEREKL RMRTLARALQ
110 120 130 140 150
ELRRFLPPSV APAGQSLTKI ETLRLAIRYI GHLSALLGLS EDSLRRRRRR
160 170 180 190 200
SADAAFSHRC PQCPDGGSPS QAQMLGPSLG SAMSSGVSWG CPPACPGPLI
210 220 230 240 250
SPENLGNRIS NVDPRVTPPY CPQIQSPLHQ SLERAADSSP WAPPQACPGM
260 270 280 290 300
QMSPEPRNKT GHWTQSTEPA ELTKVYQSLS VSPEPRLSLG SPLLLPRPSC
310 320 330 340 350
QRLQPQPQPQ PQWGCWGHDA EVLSTSEDQG SSPALQLPVA SPTPSSGLQL
360 370
SGCPELWQED LEGPPLNIFY
Length:370
Mass (Da):39,789
Last modified:July 1, 1997 - v1
Checksum:i31CAB72E2398150D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6H5V4A6H5V4_MOUSE
Mesoderm posterior 2
Mesp2 mCG_19460
370Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107P → G in BAE27401 (PubMed:16141072).Curated1
Sequence conflicti215R → W in BAE27401 (PubMed:16141072).Curated1
Sequence conflicti286R → C in BAE27401 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71125 mRNA Translation: AAB51199.1
AK146740 mRNA Translation: BAE27401.1
RefSeqiNP_032615.2, NM_008589.2
UniGeneiMm.31472

Genome annotation databases

GeneIDi17293
KEGGimmu:17293

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71125 mRNA Translation: AAB51199.1
AK146740 mRNA Translation: BAE27401.1
RefSeqiNP_032615.2, NM_008589.2
UniGeneiMm.31472

3D structure databases

ProteinModelPortaliO08574
SMRiO08574
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiO08574
PhosphoSitePlusiO08574

Proteomic databases

PRIDEiO08574

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17293
KEGGimmu:17293

Organism-specific databases

CTDi145873
MGIiMGI:1096325 Mesp2

Phylogenomic databases

HOVERGENiHBG096371
InParanoidiO08574
KOiK09076
PhylomeDBiO08574

Miscellaneous databases

PROiPR:O08574
SOURCEiSearch...

Gene expression databases

CleanExiMM_MESP2

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMESP2_MOUSE
AccessioniPrimary (citable) accession number: O08574
Secondary accession number(s): Q3UIV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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