UniProtKB - O08574 (MESP2_MOUSE)
Mesoderm posterior protein 2
Mesp2
Functioni
Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. May play a role with Tcf15 in the differentiation of myotomal and sclerotomal cells by regulating Pax family genes. Controls also the expression of the protocadherin PCDH8/PAPC, EPHA4, RIPPLY2, NOTCH2, FGFR1, and CER1. Binds to the E-boxes within the EPH4A and RIPPLY2 enhancers.
10 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- heart morphogenesis Source: GO_Central
- mesodermal cell migration Source: MGI
- mesoderm formation Source: GO_Central
- Notch signaling pathway Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- regulation of transcription by RNA polymerase II Source: GO_Central
- signal transduction involved in regulation of gene expression Source: MGI
- somite rostral/caudal axis specification Source: MGI
- somitogenesis Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Notch signaling pathway, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Mesoderm posterior protein 2 |
Gene namesi | Name:Mesp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1096325, Mesp2 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation2 Publications
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 70 – 73 | RRTR → AATA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 145-A--A-150. 1 Publication | 4 | |
Mutagenesisi | 145 – 150 | RRRRRR → AAAAAA: Localizes in nucleus and cytoplasm. Localizes mainly in cytoplasm; when associated with 70-A--A-73. 1 Publication | 6 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000296302 | 1 – 370 | Mesoderm posterior protein 2Add BLAST | 370 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | O08574 |
PTM databases
iPTMneti | O08574 |
PhosphoSitePlusi | O08574 |
Expressioni
Developmental stagei
Inductioni
Interactioni
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Miscellaneous databases
RNActi | O08574, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 133 | bHLHPROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 89 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 231 – 265 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 325 – 350 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 326 – 330 | May contain a degradation domain | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 54 – 70 | Polar residuesSequence analysisAdd BLAST | 17 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
InParanoidi | O08574 |
OrthoDBi | 1072866at2759 |
PhylomeDBi | O08574 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040259, Mesogenin/MesP |
PANTHERi | PTHR20937, PTHR20937, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAQSSPPQSL QGLVPLGLLP GLGLGSAIGL HVSGLVLRFV RFLPFYATRR
60 70 80 90 100
PSQPAGPARS TRTTQATAPR RTRPAPAGGQ RQSASEREKL RMRTLARALQ
110 120 130 140 150
ELRRFLPPSV APAGQSLTKI ETLRLAIRYI GHLSALLGLS EDSLRRRRRR
160 170 180 190 200
SADAAFSHRC PQCPDGGSPS QAQMLGPSLG SAMSSGVSWG CPPACPGPLI
210 220 230 240 250
SPENLGNRIS NVDPRVTPPY CPQIQSPLHQ SLERAADSSP WAPPQACPGM
260 270 280 290 300
QMSPEPRNKT GHWTQSTEPA ELTKVYQSLS VSPEPRLSLG SPLLLPRPSC
310 320 330 340 350
QRLQPQPQPQ PQWGCWGHDA EVLSTSEDQG SSPALQLPVA SPTPSSGLQL
360 370
SGCPELWQED LEGPPLNIFY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA6H5V4 | A6H5V4_MOUSE | Mesoderm posterior 2 | Mesp2 | 370 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | P → G in BAE27401 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 215 | R → W in BAE27401 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 286 | R → C in BAE27401 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71125 mRNA Translation: AAB51199.1 AK146740 mRNA Translation: BAE27401.1 |
RefSeqi | NP_032615.2, NM_008589.2 |
Genome annotation databases
GeneIDi | 17293 |
KEGGi | mmu:17293 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71125 mRNA Translation: AAB51199.1 AK146740 mRNA Translation: BAE27401.1 |
RefSeqi | NP_032615.2, NM_008589.2 |
3D structure databases
AlphaFoldDBi | O08574 |
SMRi | O08574 |
ModBasei | Search... |
PTM databases
iPTMneti | O08574 |
PhosphoSitePlusi | O08574 |
Proteomic databases
PRIDEi | O08574 |
Protocols and materials databases
DNASUi | 17293 |
Genome annotation databases
GeneIDi | 17293 |
KEGGi | mmu:17293 |
Organism-specific databases
CTDi | 145873 |
MGIi | MGI:1096325, Mesp2 |
Phylogenomic databases
eggNOGi | KOG4029, Eukaryota |
InParanoidi | O08574 |
OrthoDBi | 1072866at2759 |
PhylomeDBi | O08574 |
Miscellaneous databases
BioGRID-ORCSi | 17293, 1 hit in 73 CRISPR screens |
PROi | PR:O08574 |
RNActi | O08574, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040259, Mesogenin/MesP |
PANTHERi | PTHR20937, PTHR20937, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MESP2_MOUSE | |
Accessioni | O08574Primary (citable) accession number: O08574 Secondary accession number(s): Q3UIV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2007 |
Last sequence update: | July 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot