UniProtKB - O08561 (PI4KB_RAT)
Phosphatidylinositol 4-kinase beta
Pi4kb
Functioni
Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP) (PubMed:8973579).
May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity).
Involved in Golgi-to-plasma membrane trafficking (PubMed:8973579).
By similarity1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H+1 PublicationEC:2.7.1.671 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Activity regulationi
GO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
- 1-phosphatidylinositol 4-kinase activity Source: RGD
- ATP binding Source: UniProtKB-KW
- phosphatidylinositol kinase activity Source: GO_Central
GO - Biological processi
- phosphatidylinositol-mediated signaling Source: GO_Central
- phosphatidylinositol phosphate biosynthetic process Source: GO_Central
- signal transduction Source: RGD
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1660514, Synthesis of PIPs at the Golgi membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4-kinase beta (EC:2.7.1.671 Publication)Short name: PI4K-beta Short name: PI4Kbeta Short name: PtdIns 4-kinase beta |
Gene namesi | Name:Pi4kb Synonyms:Pik4cb |
Organismi | Rattus norvegicus (Rat)Imported |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621214, Pi4kb |
Subcellular locationi
Endoplasmic reticulum
- Rough endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- Mitochondrion outer membrane By similarity; Peripheral membrane protein By similarity
Golgi apparatus
- Golgi apparatus 3 Publications
- Golgi apparatus membrane By similarity
Other locations
- Endomembrane system By similarity
Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3, GGA2 is also involved in the recruitment.By similarity
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- rough endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: RGD
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: RGD
Other locations
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000088831 | 2 – 816 | Phosphatidylinositol 4-kinase betaAdd BLAST | 815 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylglycineBy similarity | 1 | |
Modified residuei | 258 | PhosphoserineBy similarity | 1 | |
Modified residuei | 263 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 266 | PhosphoserineBy similarity | 1 | |
Modified residuei | 275 | PhosphoserineBy similarity | 1 | |
Modified residuei | 277 | PhosphoserineBy similarity | 1 | |
Modified residuei | 284 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | PhosphoserineBy similarity | 1 | |
Modified residuei | 428 | PhosphoserineCombined sources | 1 | |
Modified residuei | 438 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 511 | PhosphoserineCombined sources | 1 | |
Modified residuei | 517 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 519 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | O08561 |
PaxDbi | O08561 |
PTM databases
iPTMneti | O08561 |
PhosphoSitePlusi | O08561 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000021024, Expressed in frontal cortex and 21 other tissues |
ExpressionAtlasi | O08561, baseline and differential |
Genevisiblei | O08561, RN |
Interactioni
Subunit structurei
Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (By similarity).
Interacts with NCS1/FREQ in a calcium-independent manner.
Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (PubMed:19458041).
Interacts with ACBD3.
Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (By similarity).
Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane (By similarity).
Interacts with ATG9A.
By similarity1 PublicationGO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
Protein-protein interaction databases
IntActi | O08561, 4 interactors |
MINTi | O08561 |
STRINGi | 10116.ENSRNOP00000039342 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 61 – 242 | PIK helicalPROSITE-ProRule annotationAdd BLAST | 182 | |
Domaini | 535 – 801 | PI3K/PI4K catalyticPROSITE-ProRule annotationAdd BLAST | 267 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 2 – 68 | Interaction with ACBD3By similarityAdd BLAST | 67 | |
Regioni | 99 – 121 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 250 – 318 | DisorderedSequence analysisAdd BLAST | 69 | |
Regioni | 541 – 547 | G-loopPROSITE-ProRule annotation | 7 | |
Regioni | 668 – 676 | Catalytic loopPROSITE-ProRule annotation | 9 | |
Regioni | 687 – 711 | Activation loopPROSITE-ProRule annotationAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 277 – 294 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0903, Eukaryota |
GeneTreei | ENSGT00550000074892 |
HOGENOMi | CLU_002446_6_0_1 |
InParanoidi | O08561 |
PhylomeDBi | O08561 |
TreeFami | TF102042 |
Family and domain databases
Gene3Di | 1.10.1070.11, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000403, PI3/4_kinase_cat_dom IPR036940, PI3/4_kinase_cat_sf IPR018936, PI3/4_kinase_CS IPR001263, PI3K_accessory_dom IPR015433, PI_Kinase |
PANTHERi | PTHR10048, PTHR10048, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
SMARTi | View protein in SMART SM00146, PI3Kc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00915, PI3_4_KINASE_1, 1 hit PS00916, PI3_4_KINASE_2, 1 hit PS50290, PI3_4_KINASE_3, 1 hit PS51545, PIK_HELICAL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGDMVVEPAT LKPTSEPTPS PSGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAISS KGSPLELVNG DGVDNEIRCL DDPPTEIREE
110 120 130 140 150
EDEMEPGVVS GTAKGTRRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPTLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTNVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LNGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810
TTKLYDGFQY LTNGIM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JYH1 | A0A0G2JYH1_RAT | Phosphatidylinositol 4-kinase beta | Pi4kb | 841 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84667 mRNA Translation: BAA18969.1 |
RefSeqi | NP_112345.1, NM_031083.1 |
Genome annotation databases
Ensembli | ENSRNOT00000040501; ENSRNOP00000039342; ENSRNOG00000021024 |
GeneIDi | 81747 |
KEGGi | rno:81747 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84667 mRNA Translation: BAA18969.1 |
RefSeqi | NP_112345.1, NM_031083.1 |
3D structure databases
SMRi | O08561 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08561, 4 interactors |
MINTi | O08561 |
STRINGi | 10116.ENSRNOP00000039342 |
PTM databases
iPTMneti | O08561 |
PhosphoSitePlusi | O08561 |
Proteomic databases
jPOSTi | O08561 |
PaxDbi | O08561 |
Genome annotation databases
Ensembli | ENSRNOT00000040501; ENSRNOP00000039342; ENSRNOG00000021024 |
GeneIDi | 81747 |
KEGGi | rno:81747 |
Organism-specific databases
CTDi | 5298 |
RGDi | 621214, Pi4kb |
Phylogenomic databases
eggNOGi | KOG0903, Eukaryota |
GeneTreei | ENSGT00550000074892 |
HOGENOMi | CLU_002446_6_0_1 |
InParanoidi | O08561 |
PhylomeDBi | O08561 |
TreeFami | TF102042 |
Enzyme and pathway databases
Reactomei | R-RNO-1660514, Synthesis of PIPs at the Golgi membrane |
Miscellaneous databases
PROi | PR:O08561 |
Gene expression databases
Bgeei | ENSRNOG00000021024, Expressed in frontal cortex and 21 other tissues |
ExpressionAtlasi | O08561, baseline and differential |
Genevisiblei | O08561, RN |
Family and domain databases
Gene3Di | 1.10.1070.11, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000403, PI3/4_kinase_cat_dom IPR036940, PI3/4_kinase_cat_sf IPR018936, PI3/4_kinase_CS IPR001263, PI3K_accessory_dom IPR015433, PI_Kinase |
PANTHERi | PTHR10048, PTHR10048, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
SMARTi | View protein in SMART SM00146, PI3Kc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00915, PI3_4_KINASE_1, 1 hit PS00916, PI3_4_KINASE_2, 1 hit PS50290, PI3_4_KINASE_3, 1 hit PS51545, PIK_HELICAL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PI4KB_RAT | |
Accessioni | O08561Primary (citable) accession number: O08561 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 14, 2003 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families