UniProtKB - O08557 (DDAH1_RAT)
Protein
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Gene
Ddah1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication
Catalytic activityi
- EC:3.5.3.18
Activity regulationi
Inhibited by zinc ions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 30 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 73 | SubstrateBy similarity | 1 | |
Binding sitei | 78 | SubstrateBy similarity | 1 | |
Binding sitei | 79 | SubstrateBy similarity | 1 | |
Binding sitei | 98 | SubstrateBy similarity | 1 | |
Binding sitei | 145 | SubstrateBy similarity | 1 | |
Active sitei | 173 | Proton donorBy similarity | 1 | |
Binding sitei | 268 | Substrate; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 274 | NucleophileBy similarity | 1 | |
Metal bindingi | 274 | ZincBy similarity | 1 |
GO - Molecular functioni
- amino acid binding Source: RGD
- dimethylargininase activity Source: RGD
- hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- arginine catabolic process Source: RGD
- arginine metabolic process Source: RGD
- citrulline metabolic process Source: UniProtKB
- negative regulation of cell population proliferation Source: RGD
- negative regulation of cellular response to hypoxia Source: RGD
- negative regulation of vascular permeability Source: RGD
- positive regulation of angiogenesis Source: RGD
- positive regulation of nitric oxide biosynthetic process Source: RGD
- regulation of systemic arterial blood pressure Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.3.18, 5301 |
Reactomei | R-RNO-203615, eNOS activation |
SABIO-RKi | O08557 |
Names & Taxonomyi
Protein namesi | Recommended name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)Short name: DDAH-1 Short name: Dimethylarginine dimethylaminohydrolase 1 Alternative name(s): DDAHI Dimethylargininase-1 |
Gene namesi | Name:Ddah1 Synonyms:Ddah |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 70968, Ddah1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000171120 | 2 – 285 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1Add BLAST | 284 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 33 | PhosphoserineCombined sources | 1 | |
Modified residuei | 222 | S-nitrosocysteineBy similarity | 1 | |
Modified residuei | 274 | S-nitrosocysteineBy similarity | 1 |
Keywords - PTMi
Acetylation, Phosphoprotein, S-nitrosylationProteomic databases
jPOSTi | O08557 |
PaxDbi | O08557 |
PRIDEi | O08557 |
PTM databases
iPTMneti | O08557 |
PhosphoSitePlusi | O08557 |
SwissPalmi | O08557 |
Expressioni
Tissue specificityi
Detected in red blood cells (at protein level). Widely distributed, high amounts found in kidney, brain, aorta and pancreas.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000014613, Expressed in adult mammalian kidney and 21 other tissues |
Genevisiblei | O08557, RN |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
IntActi | O08557, 1 interactor |
STRINGi | 10116.ENSRNOP00000044971 |
Chemistry databases
BindingDBi | O08557 |
Family & Domainsi
Sequence similaritiesi
Belongs to the DDAH family.Curated
Phylogenomic databases
eggNOGi | ENOG502QWPA, Eukaryota |
GeneTreei | ENSGT00940000157892 |
HOGENOMi | CLU_067923_0_0_1 |
InParanoidi | O08557 |
OMAi | GPDVICV |
OrthoDBi | 1469762at2759 |
PhylomeDBi | O08557 |
Family and domain databases
InterProi | View protein in InterPro IPR033199, DDAH IPR033197, DDAH1 |
PANTHERi | PTHR12737, PTHR12737, 1 hit PTHR12737:SF17, PTHR12737:SF17, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O08557-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGLSHPSVF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHQLY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKTDS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86041 mRNA Translation: BAA18993.1 |
RefSeqi | NP_071633.1, NM_022297.2 |
Genome annotation databases
Ensembli | ENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613 |
GeneIDi | 64157 |
KEGGi | rno:64157 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86041 mRNA Translation: BAA18993.1 |
RefSeqi | NP_071633.1, NM_022297.2 |
3D structure databases
SMRi | O08557 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O08557, 1 interactor |
STRINGi | 10116.ENSRNOP00000044971 |
Chemistry databases
BindingDBi | O08557 |
ChEMBLi | CHEMBL4196 |
PTM databases
iPTMneti | O08557 |
PhosphoSitePlusi | O08557 |
SwissPalmi | O08557 |
Proteomic databases
jPOSTi | O08557 |
PaxDbi | O08557 |
PRIDEi | O08557 |
Genome annotation databases
Ensembli | ENSRNOT00000045016; ENSRNOP00000044971; ENSRNOG00000014613 |
GeneIDi | 64157 |
KEGGi | rno:64157 |
Organism-specific databases
CTDi | 23576 |
RGDi | 70968, Ddah1 |
Phylogenomic databases
eggNOGi | ENOG502QWPA, Eukaryota |
GeneTreei | ENSGT00940000157892 |
HOGENOMi | CLU_067923_0_0_1 |
InParanoidi | O08557 |
OMAi | GPDVICV |
OrthoDBi | 1469762at2759 |
PhylomeDBi | O08557 |
Enzyme and pathway databases
BRENDAi | 3.5.3.18, 5301 |
Reactomei | R-RNO-203615, eNOS activation |
SABIO-RKi | O08557 |
Miscellaneous databases
PROi | PR:O08557 |
Gene expression databases
Bgeei | ENSRNOG00000014613, Expressed in adult mammalian kidney and 21 other tissues |
Genevisiblei | O08557, RN |
Family and domain databases
InterProi | View protein in InterPro IPR033199, DDAH IPR033197, DDAH1 |
PANTHERi | PTHR12737, PTHR12737, 1 hit PTHR12737:SF17, PTHR12737:SF17, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDAH1_RAT | |
Accessioni | O08557Primary (citable) accession number: O08557 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families