Sec22b

Functionⁱ

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.

GO - Molecular functionⁱ

SNAP receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
syntaxin binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ER to Golgi vesicle-mediated transport
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
negative regulation of autophagosome assembly Source: MGI
positive regulation of protein catabolic process Source: MGI
protein transport Source: UniProtKB-KW
retrograde vesicle-mediated transport, Golgi to ER
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
vesicle fusion with Golgi apparatus
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
vesicle-mediated transport
Source: MGI
Inferred from direct assayⁱ
- 9094723

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	ER-Golgi transport, Protein transport, Transport

Biological process

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1236974 ER-Phagosome pathway R-MMU-204005 COPII-mediated vesicle transport R-MMU-5694530 Cargo concentration in the ER R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Reactomeⁱ

R-MMU-1236974 ER-Phagosome pathway
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Vesicle-trafficking protein SEC22b Alternative name(s): ER-Golgi SNARE of 24 kDa Short name: ERS-24 Short name: ERS24 SEC22 vesicle-trafficking protein homolog B SEC22 vesicle-trafficking protein-like 1 Short name: mSec22b
Gene namesⁱ	Name:Sec22b Synonyms:Sec22l1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:1338759 Sec22b

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Topological domainⁱ	1 – 194	CytoplasmicSequence analysisAdd BLAST		194
Transmembraneⁱ	195 – 215	Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST		21

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000206771	1 – 215	Vesicle-trafficking protein SEC22bAdd BLAST		215

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	38	N6-acetyllysineBy similarity	1
Modified residueⁱ	137	PhosphoserineCombined sources	1
Modified residueⁱ	140	PhosphothreonineBy similarity	1
Modified residueⁱ	164	PhosphoserineBy similarity	1
Modified residueⁱ	168	PhosphoserineCombined sources	1
Modified residueⁱ	174	PhosphoserineBy similarity	1
Modified residueⁱ	177	PhosphoserineBy similarity	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O08547
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O08547
PaxDbⁱ	O08547
PeptideAtlas More...PeptideAtlasⁱ	O08547
PRIDEⁱ	O08547
TopDownProteomicsⁱ	O08547

PTM databases

iPTMnetⁱ	O08547
PhosphoSitePlusⁱ	O08547
SwissPalmⁱ	O08547

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000027879 Expressed in 285 organ(s), highest expression level in cumulus cell
ExpressionAtlasⁱ	O08547 baseline and differential
Genevisibleⁱ	O08547 MM

Interactionⁱ

Subunit structureⁱ

Interacts with STX17. Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B as subunit of either complex (By similarity). Interacts with the COPII Sec23/24 complex composed of SEC23A and SEC24A; recruits SEC22B into COPII-coated vesicles to allow its transport from the endoplasmic reticulum to the Golgi (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Stx1a	O35526	4	EBI-8400083,EBI-400878

With

Entry

#Exp.

IntAct

Notes

Stx1a

O35526

4

EBI-8400083,EBI-400878

GO - Molecular functionⁱ

SNAP receptor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
syntaxin binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	203149, 4 interactors
Database of interacting proteins More...DIPⁱ	DIP-60852N
IntActⁱ	O08547, 3 interactors
Molecular INTeraction database More...MINTⁱ	O08547
STRINGⁱ	10090.ENSMUSP00000029476

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	4 – 9	Combined sources	6
Turnⁱ	10 – 13	Combined sources	4
Beta strandⁱ	14 – 19	Combined sources	6
Helixⁱ	30 – 43	Combined sources	14
Beta strandⁱ	46 – 48	Combined sources	3
Beta strandⁱ	50 – 56	Combined sources	7
Beta strandⁱ	59 – 66	Combined sources	8
Beta strandⁱ	69 – 76	Combined sources	8
Helixⁱ	81 – 99	Combined sources	19
Turnⁱ	100 – 102	Combined sources	3
Helixⁱ	103 – 105	Combined sources	3
Turnⁱ	109 – 112	Combined sources	4
Helixⁱ	113 – 115	Combined sources	3
Helixⁱ	116 – 123	Combined sources	8
Turnⁱ	124 – 126	Combined sources	3
Turnⁱ	129 – 131	Combined sources	3
Beta strandⁱ	150 – 152	Combined sources	3
Helixⁱ	153 – 156	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	O08547
SMRⁱ	O08547
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

O08547

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	6 – 119	LonginPROSITE-ProRule annotationAdd BLAST		114
Domainⁱ	134 – 194	v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST		61

Sequence similaritiesⁱ

Belongs to the synaptobrevin family.Curated

Keywords - Domainⁱ

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0862 Eukaryota COG5143 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046833
HOGENOMⁱ	HOG000044957
HOVERGENⁱ	HBG052748
InParanoidⁱ	O08547
KEGG Orthology (KO) More...KOⁱ	K08517
OMAⁱ	DVTRIMV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MPE
PhylomeDBⁱ	O08547
TreeFamⁱ	TF105933

Family and domain databases

InterProⁱ	View protein in InterPro IPR011012 Longin-like_dom_sf IPR010908 Longin_dom IPR001388 Synaptobrevin
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13774 Longin, 1 hit PF00957 Synaptobrevin, 1 hit
PRINTSⁱ	PR00219 SYNAPTOBREVN
SMARTⁱ	View protein in SMART SM01270 Longin, 1 hit
SUPFAMⁱ	SSF64356 SSF64356, 1 hit
PROSITEⁱ	View protein in PROSITE PS50859 LONGIN, 1 hit PS50892 V_SNARE, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

O08547-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT 
        60         70         80         90        100
RCTLEAGAMT FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG 
       110        120        130        140        150
KKVPTVSRPY SFIEFDTFIQ KTKKLYIDSR ARRNLGSINT ELQDVQRIMV 
       160        170        180        190        200
ANIEEVLQRG EALSALDSKA NNLSSLSKKY RQDAKYLNMR STYAKLAAVA 
       210 
VFFIMLIVYV RFWWL

Length:215

Mass (Da):24,741

Last modified:January 23, 2007 - v3

Checksum:ⁱ29B4C55961C5A044

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

E9Q6R3	E9Q6R3_MOUSE	Vesicle-trafficking protein SEC22b Vesicle-trafficking protein SEC22b	Sec22b	166	Annotation score:
D6RES2	D6RES2_MOUSE	Vesicle-trafficking protein SEC22b Vesicle-trafficking protein SEC22b	Sec22b	42	Annotation score:
A0A0G2JF08	A0A0G2JF08_MOUSE	Vesicle-trafficking protein SEC22b Vesicle-trafficking protein SEC22b	Sec22b	62	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	189	M → I in AAH09024 (PubMed:15489334).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U91538 mRNA Translation: AAC53130.1 AK017237 mRNA Translation: BAB30646.1 AK034973 mRNA Translation: BAC28899.1 AK037918 mRNA Translation: BAC29901.1 AK088514 mRNA Translation: BAC40397.1 AK089928 mRNA Translation: BAC40999.1 BC009024 mRNA Translation: AAH09024.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS17658.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035472.1, NM_011342.4
UniGeneⁱ	Mm.2551 Mm.486379

Genome annotation databases

Ensemblⁱ	ENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879
GeneIDⁱ	20333
KEGGⁱ	mmu:20333
UCSC genome browser More...UCSCⁱ	uc008qpk.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O08547	Vesicle-trafficking protein SEC22b		PONAB		215	UniRef100_O08547
Vesicle-trafficking protein SEC22b		RAT		215
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)		MACMU		215
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)		CALJA		215
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)		MACFA		215
+23

Protein	Similar proteins		Species	Score	Length	Source
O08547	Vesicle-trafficking protein SEC22b		PONAB		215	UniRef90_O08547
Vesicle-trafficking protein SEC22b		RAT		215
Vesicle-trafficking protein SEC22b		CHICK		215
Vesicle-trafficking protein SEC22b		CRIGR		215
SEC22 homolog B, vesicle trafficking protein (gene/pseudogene)		MACMU		215
+199

Protein	Similar proteins		Species	Score	Length	Source
O08547	Vesicle-trafficking protein SEC22b	)	HUMAN		215	UniRef50_O75396
Vesicle-trafficking protein SEC22b		XENTR		215
Vesicle-trafficking protein SEC22b		CRIGR		215
Vesicle-trafficking protein SEC22b		PONAB		215
Vesicle-trafficking protein SEC22b		RAT		215
+278

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U91538 mRNA Translation: AAC53130.1 AK017237 mRNA Translation: BAB30646.1 AK034973 mRNA Translation: BAC28899.1 AK037918 mRNA Translation: BAC29901.1 AK088514 mRNA Translation: BAC40397.1 AK089928 mRNA Translation: BAC40999.1 BC009024 mRNA Translation: AAH09024.1
CCDSⁱ	CCDS17658.1
RefSeqⁱ	NP_035472.1, NM_011342.4
UniGeneⁱ	Mm.2551 Mm.486379

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1IFQ	X-ray	2.40	A/B	2-127	[»]
	5VNE	X-ray	2.70	C	1-157	[»]
	5VNF	X-ray	2.41	C	1-157	[»]
	5VNG	X-ray	2.60	C	1-157	[»]
	5VNH	X-ray	2.60	C	1-157	[»]
	5VNI	X-ray	2.79	C	1-157	[»]
	5VNJ	X-ray	2.81	C	1-157	[»]
	5VNK	X-ray	2.55	C	1-157	[»]
	5VNL	X-ray	2.39	C	1-157	[»]
	5VNM	X-ray	2.77	C	1-157	[»]
	5VNN	X-ray	2.50	C	1-157	[»]
	5VNO	X-ray	2.90	C	1-157	[»]
ProteinModelPortalⁱ	O08547
SMRⁱ	O08547
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	203149, 4 interactors
DIPⁱ	DIP-60852N
IntActⁱ	O08547, 3 interactors
MINTⁱ	O08547
STRINGⁱ	10090.ENSMUSP00000029476

PTM databases

iPTMnetⁱ	O08547
PhosphoSitePlusⁱ	O08547
SwissPalmⁱ	O08547

Proteomic databases

EPDⁱ	O08547
MaxQBⁱ	O08547
PaxDbⁱ	O08547
PeptideAtlasⁱ	O08547
PRIDEⁱ	O08547
TopDownProteomicsⁱ	O08547

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879
GeneIDⁱ	20333
KEGGⁱ	mmu:20333
UCSCⁱ	uc008qpk.1 mouse

Organism-specific databases

CTDⁱ	9554
MGIⁱ	MGI:1338759 Sec22b

Phylogenomic databases

eggNOGⁱ	KOG0862 Eukaryota COG5143 LUCA
GeneTreeⁱ	ENSGT00510000046833
HOGENOMⁱ	HOG000044957
HOVERGENⁱ	HBG052748
InParanoidⁱ	O08547
KOⁱ	K08517
OMAⁱ	DVTRIMV
OrthoDBⁱ	EOG091G0MPE
PhylomeDBⁱ	O08547
TreeFamⁱ	TF105933

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1236974 ER-Phagosome pathway R-MMU-204005 COPII-mediated vesicle transport R-MMU-5694530 Cargo concentration in the ER R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Reactomeⁱ

R-MMU-1236974 ER-Phagosome pathway
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

EvolutionaryTraceⁱ	O08547
Protein Ontology More...PROⁱ	PR:O08547
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027879 Expressed in 285 organ(s), highest expression level in cumulus cell
ExpressionAtlasⁱ	O08547 baseline and differential
Genevisibleⁱ	O08547 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR011012 Longin-like_dom_sf IPR010908 Longin_dom IPR001388 Synaptobrevin
Pfamⁱ	View protein in Pfam PF13774 Longin, 1 hit PF00957 Synaptobrevin, 1 hit
PRINTSⁱ	PR00219 SYNAPTOBREVN
SMARTⁱ	View protein in SMART SM01270 Longin, 1 hit
SUPFAMⁱ	SSF64356 SSF64356, 1 hit
PROSITEⁱ	View protein in PROSITE PS50859 LONGIN, 1 hit PS50892 V_SNARE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SC22B_MOUSE
Accessionⁱ	O08547Primary (citable) accession number: O08547 Secondary accession number(s): Q91VU3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O08547 (SC22B_MOUSE)

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Vesicle-trafficking protein SEC22b

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Golgi apparatus

Endoplasmic reticulum

Other locations

Endoplasmic reticulum

Golgi apparatus

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ