UniProtKB - O08547 (SC22B_MOUSE)
Protein
Vesicle-trafficking protein SEC22b
Gene
Sec22b
Organism
Mus musculus (Mouse)
Status
Functioni
SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- syntaxin binding Source: MGI
GO - Biological processi
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
- negative regulation of autophagosome assembly Source: MGI
- positive regulation of protein catabolic process Source: MGI
- protein transport Source: UniProtKB-KW
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central
- vesicle fusion with Golgi apparatus Source: GO_Central
- vesicle-mediated transport Source: MGI
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-204005, COPII-mediated vesicle transport R-MMU-5694530, Cargo concentration in the ER R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Vesicle-trafficking protein SEC22bAlternative name(s): ER-Golgi SNARE of 24 kDa Short name: ERS-24 Short name: ERS24 SEC22 vesicle-trafficking protein homolog B SEC22 vesicle-trafficking protein-like 1 Short name: mSec22b |
Gene namesi | Name:Sec22b Synonyms:Sec22l1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1338759, Sec22b |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type IV membrane protein By similarity
Golgi apparatus
- cis-Golgi network membrane By similarity
- trans-Golgi network membrane By similarity
Other locations
- Endoplasmic reticulum-Golgi intermediate compartment membrane By similarity
- Melanosome By similarity
Note: Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: MGI
Golgi apparatus
- Golgi membrane Source: MGI
Other locations
- COPI-coated vesicle Source: MGI
- endoplasmic reticulum-Golgi intermediate compartment Source: MGI
- endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
- ER to Golgi transport vesicle membrane Source: MGI
- integral component of membrane Source: UniProtKB-KW
- melanosome Source: UniProtKB-SubCell
- SNARE complex Source: MGI
- synaptic vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 194 | CytoplasmicSequence analysisAdd BLAST | 194 | |
Transmembranei | 195 – 215 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206771 | 1 – 215 | Vesicle-trafficking protein SEC22bAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 38 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 164 | PhosphoserineBy similarity | 1 | |
Modified residuei | 168 | PhosphoserineCombined sources | 1 | |
Modified residuei | 174 | PhosphoserineBy similarity | 1 | |
Modified residuei | 177 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O08547 |
jPOSTi | O08547 |
MaxQBi | O08547 |
PaxDbi | O08547 |
PeptideAtlasi | O08547 |
PRIDEi | O08547 |
TopDownProteomicsi | O08547 |
PTM databases
iPTMneti | O08547 |
PhosphoSitePlusi | O08547 |
SwissPalmi | O08547 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027879, Expressed in cumulus cell and 300 other tissues |
Genevisiblei | O08547, MM |
Interactioni
Subunit structurei
Interacts with STX17.
Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B as subunit of either complex (By similarity).
Interacts with the COPII Sec23/24 complex composed of SEC23A and SEC24A; recruits SEC22B into COPII-coated vesicles to allow its transport from the endoplasmic reticulum to the Golgi (By similarity).
By similarityBinary interactionsi
O08547
With | #Exp. | IntAct |
---|---|---|
Stx1a [O35526] | 4 | EBI-8400083,EBI-400878 |
GO - Molecular functioni
- syntaxin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203149, 24 interactors |
DIPi | DIP-60852N |
IntActi | O08547, 3 interactors |
MINTi | O08547 |
STRINGi | 10090.ENSMUSP00000029476 |
Miscellaneous databases
RNActi | O08547, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O08547 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O08547 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 119 | LonginPROSITE-ProRule annotationAdd BLAST | 114 | |
Domaini | 134 – 194 | v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the synaptobrevin family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0862, Eukaryota |
GeneTreei | ENSGT00940000156349 |
HOGENOMi | CLU_054453_4_1_1 |
InParanoidi | O08547 |
OMAi | ERSYPRK |
OrthoDBi | 903466at2759 |
PhylomeDBi | O08547 |
TreeFami | TF105933 |
Family and domain databases
CDDi | cd14824, Longin, 1 hit |
InterProi | View protein in InterPro IPR011012, Longin-like_dom_sf IPR010908, Longin_dom IPR001388, Synaptobrevin IPR042855, V_SNARE_CC |
Pfami | View protein in Pfam PF13774, Longin, 1 hit PF00957, Synaptobrevin, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
SMARTi | View protein in SMART SM01270, Longin, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS50859, LONGIN, 1 hit PS50892, V_SNARE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O08547-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT
60 70 80 90 100
RCTLEAGAMT FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG
110 120 130 140 150
KKVPTVSRPY SFIEFDTFIQ KTKKLYIDSR ARRNLGSINT ELQDVQRIMV
160 170 180 190 200
ANIEEVLQRG EALSALDSKA NNLSSLSKKY RQDAKYLNMR STYAKLAAVA
210
VFFIMLIVYV RFWWL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q6R3 | E9Q6R3_MOUSE | Vesicle-trafficking protein SEC22b | Sec22b | 166 | Annotation score: | ||
A0A0G2JF08 | A0A0G2JF08_MOUSE | Vesicle-trafficking protein SEC22b | Sec22b | 62 | Annotation score: | ||
D6RES2 | D6RES2_MOUSE | Vesicle-trafficking protein SEC22b | Sec22b | 42 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 189 | M → I in AAH09024 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91538 mRNA Translation: AAC53130.1 AK017237 mRNA Translation: BAB30646.1 AK034973 mRNA Translation: BAC28899.1 AK037918 mRNA Translation: BAC29901.1 AK088514 mRNA Translation: BAC40397.1 AK089928 mRNA Translation: BAC40999.1 BC009024 mRNA Translation: AAH09024.1 |
CCDSi | CCDS17658.1 |
RefSeqi | NP_035472.1, NM_011342.4 |
Genome annotation databases
Ensembli | ENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879 |
GeneIDi | 20333 |
KEGGi | mmu:20333 |
UCSCi | uc008qpk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91538 mRNA Translation: AAC53130.1 AK017237 mRNA Translation: BAB30646.1 AK034973 mRNA Translation: BAC28899.1 AK037918 mRNA Translation: BAC29901.1 AK088514 mRNA Translation: BAC40397.1 AK089928 mRNA Translation: BAC40999.1 BC009024 mRNA Translation: AAH09024.1 |
CCDSi | CCDS17658.1 |
RefSeqi | NP_035472.1, NM_011342.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IFQ | X-ray | 2.40 | A/B | 2-127 | [»] | |
5VNE | X-ray | 2.70 | C | 1-157 | [»] | |
5VNF | X-ray | 2.41 | C | 1-157 | [»] | |
5VNG | X-ray | 2.60 | C | 1-157 | [»] | |
5VNH | X-ray | 2.60 | C | 1-157 | [»] | |
5VNI | X-ray | 2.79 | C | 1-157 | [»] | |
5VNJ | X-ray | 2.81 | C | 1-157 | [»] | |
5VNK | X-ray | 2.55 | C | 1-157 | [»] | |
5VNL | X-ray | 2.39 | C | 1-157 | [»] | |
5VNM | X-ray | 2.77 | C | 1-157 | [»] | |
5VNN | X-ray | 2.50 | C | 1-157 | [»] | |
5VNO | X-ray | 2.90 | C | 1-157 | [»] | |
SMRi | O08547 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 203149, 24 interactors |
DIPi | DIP-60852N |
IntActi | O08547, 3 interactors |
MINTi | O08547 |
STRINGi | 10090.ENSMUSP00000029476 |
PTM databases
iPTMneti | O08547 |
PhosphoSitePlusi | O08547 |
SwissPalmi | O08547 |
Proteomic databases
EPDi | O08547 |
jPOSTi | O08547 |
MaxQBi | O08547 |
PaxDbi | O08547 |
PeptideAtlasi | O08547 |
PRIDEi | O08547 |
TopDownProteomicsi | O08547 |
Protocols and materials databases
Antibodypediai | 72298, 202 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029476; ENSMUSP00000029476; ENSMUSG00000027879 |
GeneIDi | 20333 |
KEGGi | mmu:20333 |
UCSCi | uc008qpk.1, mouse |
Organism-specific databases
CTDi | 9554 |
MGIi | MGI:1338759, Sec22b |
Phylogenomic databases
eggNOGi | KOG0862, Eukaryota |
GeneTreei | ENSGT00940000156349 |
HOGENOMi | CLU_054453_4_1_1 |
InParanoidi | O08547 |
OMAi | ERSYPRK |
OrthoDBi | 903466at2759 |
PhylomeDBi | O08547 |
TreeFami | TF105933 |
Enzyme and pathway databases
Reactomei | R-MMU-1236974, ER-Phagosome pathway R-MMU-204005, COPII-mediated vesicle transport R-MMU-5694530, Cargo concentration in the ER R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic |
Miscellaneous databases
BioGRID-ORCSi | 20333, 9 hits in 18 CRISPR screens |
ChiTaRSi | Sec22b, mouse |
EvolutionaryTracei | O08547 |
PROi | PR:O08547 |
RNActi | O08547, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027879, Expressed in cumulus cell and 300 other tissues |
Genevisiblei | O08547, MM |
Family and domain databases
CDDi | cd14824, Longin, 1 hit |
InterProi | View protein in InterPro IPR011012, Longin-like_dom_sf IPR010908, Longin_dom IPR001388, Synaptobrevin IPR042855, V_SNARE_CC |
Pfami | View protein in Pfam PF13774, Longin, 1 hit PF00957, Synaptobrevin, 1 hit |
PRINTSi | PR00219, SYNAPTOBREVN |
SMARTi | View protein in SMART SM01270, Longin, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS50859, LONGIN, 1 hit PS50892, V_SNARE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SC22B_MOUSE | |
Accessioni | O08547Primary (citable) accession number: O08547 Secondary accession number(s): Q91VU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 179 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families