Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ephrin-A5

Gene

Efna5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.4 Publications

GO - Molecular functioni

  • chemorepellent activity Source: MGI
  • ephrin receptor binding Source: UniProtKB
  • neurotrophin TRKB receptor binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-2682334 EPH-Ephrin signaling
R-MMU-3928663 EPHA-mediated growth cone collapse
R-MMU-3928665 EPH-ephrin mediated repulsion of cells

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-A5
Alternative name(s):
AL-1
EPH-related receptor tyrosine kinase ligand 7
Short name:
LERK-7
Gene namesi
Name:Efna5
Synonyms:Epl7, Eplg7, Lerk7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107444 Efna5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display cataract an opacification of the lens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000837921 – 203Ephrin-A5Add BLAST183
PropeptideiPRO_0000008380204 – 228Removed in mature formSequence analysisAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi37N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi62 ↔ 102PROSITE-ProRule annotation1 Publication
Disulfide bondi90 ↔ 151PROSITE-ProRule annotation1 Publication
Glycosylationi162N-linked (GlcNAc...) asparagine; atypical1 Publication1
Lipidationi203GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO08543
PRIDEiO08543

PTM databases

iPTMnetiO08543
PhosphoSitePlusiO08543

Expressioni

Tissue specificityi

Expressed in myogenic progenitor cells.1 Publication

Developmental stagei

In myogenic progenitor cells, highly expressed at E11.5 and ceases its expression at the late fetal stage (E17.5).1 Publication

Gene expression databases

BgeeiENSMUSG00000048915 Expressed in 289 organ(s), highest expression level in ear
CleanExiMM_EFNA5
GenevisibleiO08543 MM

Interactioni

Subunit structurei

Binds to the receptor tyrosine kinases EPHA2, EPHA3 and EPHB1. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function (By similarity). Binds to EPHB2. Interacts with EPHA8; activates EPHA8.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiO08543, 2 interactors
STRINGi10090.ENSMUSP00000076115

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO08543
SMRiO08543
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO08543

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 162Ephrin RBDPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3858 Eukaryota
ENOG4111FMJ LUCA
GeneTreeiENSGT00390000015107
HOGENOMiHOG000234373
HOVERGENiHBG051447
InParanoidiO08543
KOiK05462
OMAiCVKTIGV
OrthoDBiEOG091G0IJF
PhylomeDBiO08543

Family and domain databases

CDDicd10425 Ephrin-A_Ectodomain, 1 hit
Gene3Di2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR008972 Cupredoxin
IPR031328 Ephrin
IPR034252 Ephrin-A_Ecto
IPR019765 Ephrin_CS
IPR001799 Ephrin_RBD
PANTHERiPTHR11304 PTHR11304, 1 hit
PfamiView protein in Pfam
PF00812 Ephrin, 1 hit
PRINTSiPR01347 EPHRIN
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002533 Ephrin, 1 hit
SUPFAMiSSF49503 SSF49503, 1 hit
PROSITEiView protein in PROSITE
PS01299 EPHRIN_RBD_1, 1 hit
PS51551 EPHRIN_RBD_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Long (identifier: O08543-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLHVEMLTLL FLVLWMCVFS QDPGSKVVAD RYAVYWNSSN PRFQRGDYHI
60 70 80 90 100
DVCINDYLDV FCPHYEDSVP EDKTERYVLY MVNFDGYSAC DHTSKGFKRW
110 120 130 140 150
ECNRPHSPNG PLKFSEKFQL FTPFSLGFEF RPGREYFYIS SAIPDNGRRS
160 170 180 190 200
CLKLKVFVRP TNSCMKTIGV HDRVFDVNDK VENSLEPADD TVHESAEPSR
210 220
GENAAQTPRI PSRLLAILLF LLAMLLTL
Length:228
Mass (Da):26,339
Last modified:July 1, 1997 - v1
Checksum:i85439F5337420022
GO
Isoform Short (identifier: O08543-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-189: Missing.

Show »
Length:201
Mass (Da):23,338
Checksum:iC5F5E78FD6674316
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001449163 – 189Missing in isoform Short. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90664 mRNA Translation: AAB50239.1
U90665 mRNA Translation: AAB50240.1
BC040218 mRNA Translation: AAH40218.1
CCDSiCCDS28934.1 [O08543-2]
CCDS28935.1 [O08543-1]
RefSeqiNP_034239.1, NM_010109.3 [O08543-2]
NP_997537.1, NM_207654.2 [O08543-1]
UniGeneiMm.401670
Mm.7978

Genome annotation databases

EnsembliENSMUST00000076840; ENSMUSP00000076115; ENSMUSG00000048915 [O08543-1]
ENSMUST00000078839; ENSMUSP00000077883; ENSMUSG00000048915 [O08543-2]
GeneIDi13640
KEGGimmu:13640
UCSCiuc008dfg.2 mouse [O08543-1]
uc008dfh.2 mouse [O08543-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90664 mRNA Translation: AAB50239.1
U90665 mRNA Translation: AAB50240.1
BC040218 mRNA Translation: AAH40218.1
CCDSiCCDS28934.1 [O08543-2]
CCDS28935.1 [O08543-1]
RefSeqiNP_034239.1, NM_010109.3 [O08543-2]
NP_997537.1, NM_207654.2 [O08543-1]
UniGeneiMm.401670
Mm.7978

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SHWX-ray2.20A28-164[»]
1SHXX-ray2.10A/B28-164[»]
ProteinModelPortaliO08543
SMRiO08543
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO08543, 2 interactors
STRINGi10090.ENSMUSP00000076115

PTM databases

iPTMnetiO08543
PhosphoSitePlusiO08543

Proteomic databases

PaxDbiO08543
PRIDEiO08543

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076840; ENSMUSP00000076115; ENSMUSG00000048915 [O08543-1]
ENSMUST00000078839; ENSMUSP00000077883; ENSMUSG00000048915 [O08543-2]
GeneIDi13640
KEGGimmu:13640
UCSCiuc008dfg.2 mouse [O08543-1]
uc008dfh.2 mouse [O08543-2]

Organism-specific databases

CTDi1946
MGIiMGI:107444 Efna5

Phylogenomic databases

eggNOGiKOG3858 Eukaryota
ENOG4111FMJ LUCA
GeneTreeiENSGT00390000015107
HOGENOMiHOG000234373
HOVERGENiHBG051447
InParanoidiO08543
KOiK05462
OMAiCVKTIGV
OrthoDBiEOG091G0IJF
PhylomeDBiO08543

Enzyme and pathway databases

ReactomeiR-MMU-2682334 EPH-Ephrin signaling
R-MMU-3928663 EPHA-mediated growth cone collapse
R-MMU-3928665 EPH-ephrin mediated repulsion of cells

Miscellaneous databases

ChiTaRSiEfna5 mouse
EvolutionaryTraceiO08543
PROiPR:O08543
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048915 Expressed in 289 organ(s), highest expression level in ear
CleanExiMM_EFNA5
GenevisibleiO08543 MM

Family and domain databases

CDDicd10425 Ephrin-A_Ectodomain, 1 hit
Gene3Di2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR008972 Cupredoxin
IPR031328 Ephrin
IPR034252 Ephrin-A_Ecto
IPR019765 Ephrin_CS
IPR001799 Ephrin_RBD
PANTHERiPTHR11304 PTHR11304, 1 hit
PfamiView protein in Pfam
PF00812 Ephrin, 1 hit
PRINTSiPR01347 EPHRIN
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002533 Ephrin, 1 hit
SUPFAMiSSF49503 SSF49503, 1 hit
PROSITEiView protein in PROSITE
PS01299 EPHRIN_RBD_1, 1 hit
PS51551 EPHRIN_RBD_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFNA5_MOUSE
AccessioniPrimary (citable) accession number: O08543
Secondary accession number(s): O08544
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again