UniProtKB - O08543 (EFNA5_MOUSE)
Protein
Ephrin-A5
Gene
Efna5
Organism
Mus musculus (Mouse)
Status
Functioni
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.4 Publications
GO - Molecular functioni
- chemorepellent activity Source: MGI
- ephrin receptor binding Source: UniProtKB
- neurotrophin TRKB receptor binding Source: BHF-UCL
GO - Biological processi
- axon guidance Source: MGI
- brain development Source: MGI
- cellular response to follicle-stimulating hormone stimulus Source: MGI
- cellular response to forskolin Source: MGI
- collateral sprouting Source: BHF-UCL
- ephrin receptor signaling pathway Source: UniProtKB
- negative regulation of substrate adhesion-dependent cell spreading Source: MGI
- positive regulation of collateral sprouting Source: BHF-UCL
- positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
- positive regulation of synapse assembly Source: BHF-UCL
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of cell-cell adhesion Source: UniProtKB
- regulation of cell morphogenesis Source: MGI
- regulation of focal adhesion assembly Source: UniProtKB
- regulation of GTPase activity Source: UniProtKB
- regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: UniProtKB
- retinal ganglion cell axon guidance Source: MGI
- synaptic membrane adhesion Source: SynGO
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-MMU-2682334 EPH-Ephrin signaling R-MMU-3928663 EPHA-mediated growth cone collapse R-MMU-3928665 EPH-ephrin mediated repulsion of cells |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin-A5Alternative name(s): AL-1 EPH-related receptor tyrosine kinase ligand 7 Short name: LERK-7 |
Gene namesi | Name:Efna5 Synonyms:Epl7, Eplg7, Lerk7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107444 Efna5 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; GPI-anchor By similarity
Other locations
- caveola By similarity; GPI-anchor By similarity
Note: Compartmentalized in discrete caveolae-like membrane microdomains.By similarity
Extracellular region or secreted
- basement membrane Source: MGI
Plasma Membrane
- anchored component of external side of plasma membrane Source: UniProtKB
- caveola Source: UniProtKB-SubCell
- plasma membrane Source: UniProtKB
Other locations
- adherens junction Source: MGI
- cell periphery Source: MGI
- GABA-ergic synapse Source: SynGO
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mice display cataract an opacification of the lens.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000008379 | 21 – 203 | Ephrin-A5Add BLAST | 183 | |
PropeptideiPRO_0000008380 | 204 – 228 | Removed in mature formSequence analysisAdd BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 62 ↔ 102 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 90 ↔ 151 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 162 | N-linked (GlcNAc...) asparagine; atypical1 Publication | 1 | |
Lipidationi | 203 | GPI-anchor amidated asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | O08543 |
PRIDEi | O08543 |
PTM databases
iPTMneti | O08543 |
PhosphoSitePlusi | O08543 |
Expressioni
Tissue specificityi
Expressed in myogenic progenitor cells.1 Publication
Developmental stagei
In myogenic progenitor cells, highly expressed at 11.5 dpc and ceases its expression at the late fetal stage (17.5 dpc).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000048915 Expressed in 289 organ(s), highest expression level in ear |
Genevisiblei | O08543 MM |
Interactioni
Subunit structurei
Binds to the receptor tyrosine kinases EPHA2, EPHA3 and EPHB1. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function (By similarity). Binds to EPHB2. Interacts with EPHA8; activates EPHA8.By similarity2 Publications
GO - Molecular functioni
- chemorepellent activity Source: MGI
- ephrin receptor binding Source: UniProtKB
- neurotrophin TRKB receptor binding Source: BHF-UCL
Protein-protein interaction databases
IntActi | O08543, 2 interactors |
STRINGi | 10090.ENSMUSP00000076115 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SHW | X-ray | 2.20 | A | 28-164 | [»] | |
1SHX | X-ray | 2.10 | A/B | 28-164 | [»] | |
ProteinModelPortali | O08543 | |||||
SMRi | O08543 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O08543 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 162 | Ephrin RBDPROSITE-ProRule annotationAdd BLAST | 134 |
Sequence similaritiesi
Belongs to the ephrin family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3858 Eukaryota ENOG4111FMJ LUCA |
GeneTreei | ENSGT00940000157299 |
HOGENOMi | HOG000234373 |
HOVERGENi | HBG051447 |
InParanoidi | O08543 |
KOi | K05462 |
OMAi | CVKTIGV |
OrthoDBi | 1094764at2759 |
PhylomeDBi | O08543 |
Family and domain databases
CDDi | cd10425 Ephrin-A_Ectodomain, 1 hit |
Gene3Di | 2.60.40.420, 1 hit |
InterProi | View protein in InterPro IPR008972 Cupredoxin IPR031328 Ephrin IPR034252 Ephrin-A_Ecto IPR019765 Ephrin_CS IPR001799 Ephrin_RBD |
PANTHERi | PTHR11304 PTHR11304, 1 hit |
Pfami | View protein in Pfam PF00812 Ephrin, 1 hit |
PRINTSi | PR01347 EPHRIN |
ProDomi | View protein in ProDom or Entries sharing at least one domain PD002533 Ephrin, 1 hit |
SUPFAMi | SSF49503 SSF49503, 1 hit |
PROSITEi | View protein in PROSITE PS01299 EPHRIN_RBD_1, 1 hit PS51551 EPHRIN_RBD_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform Long (identifier: O08543-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MLHVEMLTLL FLVLWMCVFS QDPGSKVVAD RYAVYWNSSN PRFQRGDYHI
60 70 80 90 100
DVCINDYLDV FCPHYEDSVP EDKTERYVLY MVNFDGYSAC DHTSKGFKRW
110 120 130 140 150
ECNRPHSPNG PLKFSEKFQL FTPFSLGFEF RPGREYFYIS SAIPDNGRRS
160 170 180 190 200
CLKLKVFVRP TNSCMKTIGV HDRVFDVNDK VENSLEPADD TVHESAEPSR
210 220
GENAAQTPRI PSRLLAILLF LLAMLLTL
Isoform Short (identifier: O08543-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
163-189: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001449 | 163 – 189 | Missing in isoform Short. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90664 mRNA Translation: AAB50239.1 U90665 mRNA Translation: AAB50240.1 BC040218 mRNA Translation: AAH40218.1 |
CCDSi | CCDS28934.1 [O08543-2] CCDS28935.1 [O08543-1] |
RefSeqi | NP_034239.1, NM_010109.3 [O08543-2] NP_997537.1, NM_207654.2 [O08543-1] |
UniGenei | Mm.401670 Mm.7978 |
Genome annotation databases
Ensembli | ENSMUST00000076840; ENSMUSP00000076115; ENSMUSG00000048915 [O08543-1] ENSMUST00000078839; ENSMUSP00000077883; ENSMUSG00000048915 [O08543-2] |
GeneIDi | 13640 |
KEGGi | mmu:13640 |
UCSCi | uc008dfg.2 mouse [O08543-1] uc008dfh.2 mouse [O08543-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90664 mRNA Translation: AAB50239.1 U90665 mRNA Translation: AAB50240.1 BC040218 mRNA Translation: AAH40218.1 |
CCDSi | CCDS28934.1 [O08543-2] CCDS28935.1 [O08543-1] |
RefSeqi | NP_034239.1, NM_010109.3 [O08543-2] NP_997537.1, NM_207654.2 [O08543-1] |
UniGenei | Mm.401670 Mm.7978 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SHW | X-ray | 2.20 | A | 28-164 | [»] | |
1SHX | X-ray | 2.10 | A/B | 28-164 | [»] | |
ProteinModelPortali | O08543 | |||||
SMRi | O08543 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
IntActi | O08543, 2 interactors |
STRINGi | 10090.ENSMUSP00000076115 |
PTM databases
iPTMneti | O08543 |
PhosphoSitePlusi | O08543 |
Proteomic databases
PaxDbi | O08543 |
PRIDEi | O08543 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000076840; ENSMUSP00000076115; ENSMUSG00000048915 [O08543-1] ENSMUST00000078839; ENSMUSP00000077883; ENSMUSG00000048915 [O08543-2] |
GeneIDi | 13640 |
KEGGi | mmu:13640 |
UCSCi | uc008dfg.2 mouse [O08543-1] uc008dfh.2 mouse [O08543-2] |
Organism-specific databases
CTDi | 1946 |
MGIi | MGI:107444 Efna5 |
Phylogenomic databases
eggNOGi | KOG3858 Eukaryota ENOG4111FMJ LUCA |
GeneTreei | ENSGT00940000157299 |
HOGENOMi | HOG000234373 |
HOVERGENi | HBG051447 |
InParanoidi | O08543 |
KOi | K05462 |
OMAi | CVKTIGV |
OrthoDBi | 1094764at2759 |
PhylomeDBi | O08543 |
Enzyme and pathway databases
Reactomei | R-MMU-2682334 EPH-Ephrin signaling R-MMU-3928663 EPHA-mediated growth cone collapse R-MMU-3928665 EPH-ephrin mediated repulsion of cells |
Miscellaneous databases
ChiTaRSi | Efna5 mouse |
EvolutionaryTracei | O08543 |
PROi | PR:O08543 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000048915 Expressed in 289 organ(s), highest expression level in ear |
Genevisiblei | O08543 MM |
Family and domain databases
CDDi | cd10425 Ephrin-A_Ectodomain, 1 hit |
Gene3Di | 2.60.40.420, 1 hit |
InterProi | View protein in InterPro IPR008972 Cupredoxin IPR031328 Ephrin IPR034252 Ephrin-A_Ecto IPR019765 Ephrin_CS IPR001799 Ephrin_RBD |
PANTHERi | PTHR11304 PTHR11304, 1 hit |
Pfami | View protein in Pfam PF00812 Ephrin, 1 hit |
PRINTSi | PR01347 EPHRIN |
ProDomi | View protein in ProDom or Entries sharing at least one domain PD002533 Ephrin, 1 hit |
SUPFAMi | SSF49503 SSF49503, 1 hit |
PROSITEi | View protein in PROSITE PS01299 EPHRIN_RBD_1, 1 hit PS51551 EPHRIN_RBD_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | EFNA5_MOUSE | |
Accessioni | O08543Primary (citable) accession number: O08543 Secondary accession number(s): O08544 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 13, 2019 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot