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Protein

Angiopoietin-1

Gene

Angpt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme (By similarity).By similarity

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: MGI
  • signaling receptor binding Source: MGI
  • vascular endothelial growth factor receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processAngiogenesis, Differentiation

Enzyme and pathway databases

ReactomeiR-MMU-210993 Tie2 Signaling
R-MMU-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Angiopoietin-1
Short name:
ANG-1
Gene namesi
Name:Angpt1
Synonyms:Agpt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:108448 Angpt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Embryonically lethal. Embryos die at about 12.5 dpc, due to important developmental defects of the endocardium and myocardium, plus generalized defects in vascular development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000911120 – 498Angiopoietin-1Add BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi286 ↔ 315PROSITE-ProRule annotation
Glycosylationi295N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi439 ↔ 452PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO08538
PaxDbiO08538
PRIDEiO08538

PTM databases

iPTMnetiO08538
PhosphoSitePlusiO08538

Expressioni

Developmental stagei

Early in development, at E9 to E11, it is found most prominently in the heart myocardium surrounding the endocardium. Later, it becomes more widely distributed, most often in the mesenchyme surrounding developing vessels, in close association with endothelial cells.

Gene expression databases

BgeeiENSMUSG00000022309 Expressed in 178 organ(s), highest expression level in cardiac atrium
CleanExiMM_ANGPT1
GenevisibleiO08538 MM

Interactioni

Subunit structurei

Homooligomer. Interacts with TEK/TIE2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-6051N
IntActiO08538, 1 interactor
STRINGi10090.ENSMUSP00000022921

Structurei

3D structure databases

ProteinModelPortaliO08538
SMRiO08538
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini277 – 497Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST221

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili81 – 119Sequence analysisAdd BLAST39
Coiled coili153 – 261Sequence analysisAdd BLAST109

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG2579 Eukaryota
ENOG410ZYS4 LUCA
GeneTreeiENSGT00920000148944
HOVERGENiHBG001644
InParanoidiO08538
KOiK05465
OMAiVFCNMDL
OrthoDBiEOG091G03M1
TreeFamiTF336658

Family and domain databases

CDDicd00087 FReD, 1 hit
Gene3Di3.90.215.10, 1 hit
4.10.530.10, 1 hit
InterProiView protein in InterPro
IPR028843 Ang-1
IPR036056 Fibrinogen-like_C
IPR014716 Fibrinogen_a/b/g_C_1
IPR014715 Fibrinogen_a/b/g_C_2
IPR002181 Fibrinogen_a/b/g_C_dom
IPR020837 Fibrinogen_CS
PANTHERiPTHR19143:SF156 PTHR19143:SF156, 1 hit
PfamiView protein in Pfam
PF00147 Fibrinogen_C, 1 hit
SMARTiView protein in SMART
SM00186 FBG, 1 hit
SUPFAMiSSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS00514 FIBRINOGEN_C_1, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O08538-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTVFLSFAFF AAILTHIGCS NQRRNPENGG RRYNRIQHGQ CAYTFILPEH
60 70 80 90 100
DGNCRESATE QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL
110 120 130 140 150
ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET
160 170 180 190 200
QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE
210 220 230 240 250
MEGKHKEELD TLKEEKENLQ GLVSRQTFII QELEKQLSRA TNNNSILQKQ
260 270 280 290 300
QLELMDTVHN LISLCTKEGV LLKGGKREEE KPFRDCADVY QAGFNKSGIY
310 320 330 340 350
TIYFNNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN
360 370 380 390 400
PSGEYWLGNE FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN
410 420 430 440 450
YRLYLKGHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD
460 470 480 490
ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK GPSYSLRSTT MMIRPLDF
Length:498
Mass (Da):57,519
Last modified:July 27, 2011 - v2
Checksum:iFC36F905A9E79074
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262I → V in AAB50558 (PubMed:8980223).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83509 mRNA Translation: AAB50558.1
BC067410 mRNA Translation: AAH67410.1
CCDSiCCDS27450.1
RefSeqiNP_033770.2, NM_009640.4
UniGeneiMm.309336

Genome annotation databases

EnsembliENSMUST00000022921; ENSMUSP00000022921; ENSMUSG00000022309
GeneIDi11600
KEGGimmu:11600
UCSCiuc007vpc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83509 mRNA Translation: AAB50558.1
BC067410 mRNA Translation: AAH67410.1
CCDSiCCDS27450.1
RefSeqiNP_033770.2, NM_009640.4
UniGeneiMm.309336

3D structure databases

ProteinModelPortaliO08538
SMRiO08538
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6051N
IntActiO08538, 1 interactor
STRINGi10090.ENSMUSP00000022921

PTM databases

iPTMnetiO08538
PhosphoSitePlusiO08538

Proteomic databases

MaxQBiO08538
PaxDbiO08538
PRIDEiO08538

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022921; ENSMUSP00000022921; ENSMUSG00000022309
GeneIDi11600
KEGGimmu:11600
UCSCiuc007vpc.2 mouse

Organism-specific databases

CTDi284
MGIiMGI:108448 Angpt1

Phylogenomic databases

eggNOGiKOG2579 Eukaryota
ENOG410ZYS4 LUCA
GeneTreeiENSGT00920000148944
HOVERGENiHBG001644
InParanoidiO08538
KOiK05465
OMAiVFCNMDL
OrthoDBiEOG091G03M1
TreeFamiTF336658

Enzyme and pathway databases

ReactomeiR-MMU-210993 Tie2 Signaling
R-MMU-5673001 RAF/MAP kinase cascade

Miscellaneous databases

ChiTaRSiAngpt1 mouse
PROiPR:O08538
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022309 Expressed in 178 organ(s), highest expression level in cardiac atrium
CleanExiMM_ANGPT1
GenevisibleiO08538 MM

Family and domain databases

CDDicd00087 FReD, 1 hit
Gene3Di3.90.215.10, 1 hit
4.10.530.10, 1 hit
InterProiView protein in InterPro
IPR028843 Ang-1
IPR036056 Fibrinogen-like_C
IPR014716 Fibrinogen_a/b/g_C_1
IPR014715 Fibrinogen_a/b/g_C_2
IPR002181 Fibrinogen_a/b/g_C_dom
IPR020837 Fibrinogen_CS
PANTHERiPTHR19143:SF156 PTHR19143:SF156, 1 hit
PfamiView protein in Pfam
PF00147 Fibrinogen_C, 1 hit
SMARTiView protein in SMART
SM00186 FBG, 1 hit
SUPFAMiSSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS00514 FIBRINOGEN_C_1, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANGP1_MOUSE
AccessioniPrimary (citable) accession number: O08538
Secondary accession number(s): Q6NWV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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