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Protein

Hexokinase-2

Gene

Hk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Activity regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.By similarity

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30ATP 1By similarity1
Binding sitei209Glucose-6-phosphate 1By similarity1
Binding sitei232Glucose-6-phosphate 1By similarity1
Binding sitei235Substrate 1By similarity1
Binding sitei260Substrate 1By similarity1
Binding sitei449Glucose-6-phosphate 1By similarity1
Binding sitei558ATP 2Sequence analysis1
Binding sitei657Glucose-6-phosphate 2By similarity1
Binding sitei680ATP 2By similarity1
Binding sitei680Glucose-6-phosphate 2By similarity1
Binding sitei683Substrate 2By similarity1
Binding sitei708Substrate 2By similarity1
Binding sitei897Glucose-6-phosphate 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 89ATP 1Sequence analysis6
Nucleotide bindingi425 – 426ATP 1By similarity2
Nucleotide bindingi532 – 537ATP 2By similarity6
Nucleotide bindingi747 – 748ATP 2By similarity2
Nucleotide bindingi784 – 788ATP 2By similarity5
Nucleotide bindingi863 – 867ATP 2By similarity5

GO - Molecular functioni

  • ATP binding Source: MGI
  • fructokinase activity Source: GO_Central
  • glucokinase activity Source: GO_Central
  • glucose binding Source: MGI
  • hexokinase activity Source: MGI
  • mannokinase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70171 Glycolysis
SABIO-RKiO08528
UniPathwayi
UPA00242

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase type II
Short name:
HK II
Gene namesi
Name:Hk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315197 Hk2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975881 – 917Hexokinase-2Add BLAST917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO08528
MaxQBiO08528
PaxDbiO08528
PeptideAtlasiO08528
PRIDEiO08528

PTM databases

iPTMnetiO08528
PhosphoSitePlusiO08528
SwissPalmiO08528

Expressioni

Gene expression databases

BgeeiENSMUSG00000000628 Expressed in 285 organ(s), highest expression level in extra-ocular muscle
CleanExiMM_HK2
ExpressionAtlasiO08528 baseline and differential
GenevisibleiO08528 MM

Interactioni

Subunit structurei

Monomer. Interacts with TIGAR; the interaction increases hexokinase HK2 activity in a hypoxia- and HIF1A-dependent manner.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pea15Q620482EBI-8344432,EBI-8314750

Protein-protein interaction databases

BioGridi200316, 4 interactors
DIPiDIP-41484N
IntActiO08528, 5 interactors
MINTiO08528
STRINGi10090.ENSMUSP00000000642

Structurei

3D structure databases

ProteinModelPortaliO08528
SMRiO08528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 458Hexokinase 1PROSITE-ProRule annotationAdd BLAST443
Domaini464 – 906Hexokinase 2PROSITE-ProRule annotationAdd BLAST443

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 12HydrophobicAdd BLAST12
Regioni13 – 475RegulatoryAdd BLAST463
Regioni73 – 207Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST135
Regioni84 – 88Glucose-6-phosphate 1 bindingBy similarity5
Regioni155 – 156Substrate 1 bindingBy similarity2
Regioni172 – 173Substrate 1 bindingBy similarity2
Regioni208 – 447Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni208 – 209Substrate 1 bindingBy similarity2
Regioni291 – 294Substrate 1 bindingBy similarity4
Regioni413 – 415Glucose-6-phosphate 1 bindingBy similarity3
Regioni476 – 917CatalyticAdd BLAST442
Regioni521 – 655Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST135
Regioni532 – 536Glucose-6-phosphate 2 bindingBy similarity5
Regioni603 – 604Substrate 2 bindingBy similarity2
Regioni620 – 621Substrate 2 bindingBy similarity2
Regioni656 – 895Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240
Regioni656 – 657Substrate 2 bindingBy similarity2
Regioni739 – 742Substrate 2 bindingBy similarity4
Regioni861 – 863Glucose-6-phosphate 2 bindingBy similarity3

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1369 Eukaryota
COG5026 LUCA
GeneTreeiENSGT00390000017159
HOGENOMiHOG000162671
HOVERGENiHBG005020
InParanoidiO08528
KOiK00844
OrthoDBiEOG091G08MD
PhylomeDBiO08528
TreeFamiTF314238

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 4 hits
PfamiView protein in Pfam
PF00349 Hexokinase_1, 2 hits
PF03727 Hexokinase_2, 2 hits
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 2 hits
PS51748 HEXOKINASE_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O08528-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIASHMIACL FTELNQNQVQ KVDQYLYHMR LSDETLLEIS RRFRKEMEKG
60 70 80 90 100
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLRVRVTDN
110 120 130 140 150
GLQRVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKEKKLPL
160 170 180 190 200
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVDLIR KAIQRRGDFD
210 220 230 240 250
IDIVAVVNDT VGTMMTCGYD DQNCEIGLIV GTGSNACYME EMRHIDMVEG
260 270 280 290 300
DEGRMCINME WGAFGDDGTL NDIRTEFDRE IDMGSLNPGK QLFEKMISGM
310 320 330 340 350
YMGELVRLIL VKMAKAELLF QGKLSPELLT TGSFETKDVS DIEDDKDGIQ
360 370 380 390 400
KAYQILVRLG LSPLQEDCVA THRICQIVST RSASLCAATL AAVLWRIKEN
410 420 430 440 450
KGEERLRSTI GVDGSVYKKH PHFAKRLHKA VRRLVPDCDV RFLRSEDGSG
460 470 480 490 500
KGAAMVTAVA YRLADQHRAR QKTLESLKLS HEQLLEVKRR MKVEMEQGLS
510 520 530 540 550
KETHEAAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKR
560 570 580 590 600
RGVEMHNKIY SIPQEVMHGT GEELFDHIVQ CIADFLEYMG MKGVSLPLGF
610 620 630 640 650
TFSFPCQQNS LDQSILLKWT KGFKASGCEG EDVVTLLKEA IRRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCGYEDP HCEVGLIVGT GSNACYMEEM RNVELVDGEE
710 720 730 740 750
GRMCVNMEWG AFGDNGCLDD LRTVFDVAVD ELSLNPGKQR FEKMISGMYL
760 770 780 790 800
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV
810 820 830 840 850
RAILRHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDKIRENRG
860 870 880 890 900
LDNLKVTVGV DGTLYKLHPH FAKVMHETVR DLAPKCDVSF LESEDGSGKG
910
AALITAVACR IREAGQR
Length:917
Mass (Da):102,535
Last modified:July 1, 1997 - v1
Checksum:i44293E7A92D724B3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q9M6E9Q9M6_MOUSE
Phosphotransferase
Hk2
43Annotation score:
E9Q5B5E9Q5B5_MOUSE
Hexokinase-2
Hk2
889Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11666, Y11667, Y11668 Genomic DNA Translation: CAA72366.1
AJ238540 mRNA Translation: CAB72257.1
BC054472 mRNA Translation: AAH54472.1
CCDSiCCDS20263.1
RefSeqiNP_038848.1, NM_013820.3
UniGeneiMm.255848
Mm.469167

Genome annotation databases

EnsembliENSMUST00000000642; ENSMUSP00000000642; ENSMUSG00000000628
GeneIDi15277
KEGGimmu:15277
UCSCiuc009cll.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11666, Y11667, Y11668 Genomic DNA Translation: CAA72366.1
AJ238540 mRNA Translation: CAB72257.1
BC054472 mRNA Translation: AAH54472.1
CCDSiCCDS20263.1
RefSeqiNP_038848.1, NM_013820.3
UniGeneiMm.255848
Mm.469167

3D structure databases

ProteinModelPortaliO08528
SMRiO08528
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200316, 4 interactors
DIPiDIP-41484N
IntActiO08528, 5 interactors
MINTiO08528
STRINGi10090.ENSMUSP00000000642

PTM databases

iPTMnetiO08528
PhosphoSitePlusiO08528
SwissPalmiO08528

Proteomic databases

EPDiO08528
MaxQBiO08528
PaxDbiO08528
PeptideAtlasiO08528
PRIDEiO08528

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000642; ENSMUSP00000000642; ENSMUSG00000000628
GeneIDi15277
KEGGimmu:15277
UCSCiuc009cll.2 mouse

Organism-specific databases

CTDi3099
MGIiMGI:1315197 Hk2

Phylogenomic databases

eggNOGiKOG1369 Eukaryota
COG5026 LUCA
GeneTreeiENSGT00390000017159
HOGENOMiHOG000162671
HOVERGENiHBG005020
InParanoidiO08528
KOiK00844
OrthoDBiEOG091G08MD
PhylomeDBiO08528
TreeFamiTF314238

Enzyme and pathway databases

UniPathwayi
UPA00242

ReactomeiR-MMU-70171 Glycolysis
SABIO-RKiO08528

Miscellaneous databases

ChiTaRSiHk2 mouse
PROiPR:O08528
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000628 Expressed in 285 organ(s), highest expression level in extra-ocular muscle
CleanExiMM_HK2
ExpressionAtlasiO08528 baseline and differential
GenevisibleiO08528 MM

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 4 hits
PfamiView protein in Pfam
PF00349 Hexokinase_1, 2 hits
PF03727 Hexokinase_2, 2 hits
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 2 hits
PS51748 HEXOKINASE_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiHXK2_MOUSE
AccessioniPrimary (citable) accession number: O08528
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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