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UniProtKB - O08315 (RIBA_HELPY)
Protein
GTP cyclohydrolase-2
Gene
ribA
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.
1 PublicationCatalytic activityi
- GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H+ + 2 phosphateUniRule annotationEC:3.5.4.25UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
: riboflavin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.UniRule annotation This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 55 | Zinc; catalyticUniRule annotation | 1 | |
Metal bindingi | 66 | Zinc; catalyticUniRule annotation | 1 | |
Metal bindingi | 68 | Zinc; catalyticUniRule annotation | 1 | |
Binding sitei | 114 | GTPUniRule annotation | 1 | |
Active sitei | 126 | Proton acceptorUniRule annotation | 1 | |
Active sitei | 128 | NucleophileUniRule annotation | 1 | |
Binding sitei | 149 | GTPUniRule annotation | 1 | |
Binding sitei | 154 | GTPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 50 – 54 | GTPUniRule annotation | 5 | |
Nucleotide bindingi | 92 – 94 | GTPUniRule annotation | 3 |
GO - Molecular functioni
- GTP binding Source: UniProtKB-KW
- GTP cyclohydrolase II activity Source: GO_Central
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- riboflavin biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Riboflavin biosynthesis |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.4.25, 2604 |
UniPathwayi | UPA00275;UER00400 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation)Alternative name(s): GTP cyclohydrolase IIUniRule annotation |
Gene namesi | Name:ribAUniRule annotation Ordered Locus Names:HP_0802 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151761 | 1 – 192 | GTP cyclohydrolase-2Add BLAST | 192 |
Proteomic databases
PaxDbi | O08315 |
Interactioni
Protein-protein interaction databases
DIPi | DIP-3449N |
IntActi | O08315, 1 interactor |
MINTi | O08315 |
STRINGi | 85962.C694_04110 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O08315 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the GTP cyclohydrolase II family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0807, Bacteria |
OMAi | GQGFILY |
PhylomeDBi | O08315 |
Family and domain databases
CDDi | cd00641, GTP_cyclohydro2, 1 hit |
Gene3Di | 3.40.50.10990, 1 hit |
HAMAPi | MF_00179, RibA, 1 hit |
InterProi | View protein in InterPro IPR032677, GTP_cyclohydro_II IPR000926, RibA IPR036144, RibA-like_sf |
Pfami | View protein in Pfam PF00925, GTP_cyclohydro2, 1 hit |
SUPFAMi | SSF142695, SSF142695, 1 hit |
TIGRFAMsi | TIGR00505, ribA, 1 hit |
i Sequence
Sequence statusi: Complete.
O08315-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLEVSNQA KLPTQFGEFY IQCFREKGSN GSKDHLVVFT PNFSQNPLVR
60 70 80 90 100
LHSECLTGDA LGSQKCDCGG ALQMALERIS KEGGLVIYLR QEGRGIGLFN
110 120 130 140 150
KVNAYALQDK GYDTIQANEM IGFKDDERDY SVAGEILEYY RIKKMRLLTN
160 170 180 190
NPKKIAALEK YAEVTRESLI VCANEHNQGY LEVKKLKMGH LL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | V → I in CAA72785 (PubMed:9574900).Curated | 1 | |
Sequence conflicti | 44 | S → P in CAA72785 (PubMed:9574900).Curated | 1 | |
Sequence conflicti | 72 | L → F in CAA72785 (PubMed:9574900).Curated | 1 | |
Sequence conflicti | 75 | A → P in CAA72785 (PubMed:9574900).Curated | 1 | |
Sequence conflicti | 98 – 100 | LFN → YLT (PubMed:9574900).Curated | 3 | |
Sequence conflicti | 102 | V → S (PubMed:9574900).Curated | 1 | |
Sequence conflicti | 141 | R → H in CAA72785 (PubMed:9574900).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07851.1 Y12062 Genomic DNA Translation: CAA72785.1 |
PIRi | B64620 |
RefSeqi | NP_207595.1, NC_000915.1 WP_000825074.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07851; AAD07851; HP_0802 |
KEGGi | hpy:HP_0802 |
PATRICi | fig|85962.47.peg.854 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07851.1 Y12062 Genomic DNA Translation: CAA72785.1 |
PIRi | B64620 |
RefSeqi | NP_207595.1, NC_000915.1 WP_000825074.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RL4 | X-ray | 2.20 | A/B | 1-192 | [»] | |
SMRi | O08315 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3449N |
IntActi | O08315, 1 interactor |
MINTi | O08315 |
STRINGi | 85962.C694_04110 |
Proteomic databases
PaxDbi | O08315 |
Genome annotation databases
EnsemblBacteriai | AAD07851; AAD07851; HP_0802 |
KEGGi | hpy:HP_0802 |
PATRICi | fig|85962.47.peg.854 |
Phylogenomic databases
eggNOGi | COG0807, Bacteria |
OMAi | GQGFILY |
PhylomeDBi | O08315 |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00400 |
BRENDAi | 3.5.4.25, 2604 |
Family and domain databases
CDDi | cd00641, GTP_cyclohydro2, 1 hit |
Gene3Di | 3.40.50.10990, 1 hit |
HAMAPi | MF_00179, RibA, 1 hit |
InterProi | View protein in InterPro IPR032677, GTP_cyclohydro_II IPR000926, RibA IPR036144, RibA-like_sf |
Pfami | View protein in Pfam PF00925, GTP_cyclohydro2, 1 hit |
SUPFAMi | SSF142695, SSF142695, 1 hit |
TIGRFAMsi | TIGR00505, ribA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIBA_HELPY | |
Accessioni | O08315Primary (citable) accession number: O08315 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families