UniProtKB - O07431 (CAMT_MYCTU)
Protein
Catechol O-methyltransferase
Gene
Rv0187
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catechol O-methyltransferase that can use various catechol-like compounds such as gallic acid (GA), 3,4-dihydroxy-5-methoxy-benzoic acid (5OMeBA), protocatechuic acid (PCA), 3,4-dihydroxy-benzaldehyde (DHA), dopamine, caffeic acid (CA), luteolin, quercetin, and 5-hydroxyuridine.1 Publication
Catalytic activityi
- EC:2.1.1.61 Publication
Cofactori
a divalent metal cation1 Publication
Activity regulationi
Inhibited by EDTA.1 Publication
Kineticsi
kcat is 0.080 min(-1) with 5OMeBA as substrate. kcat is 0.22 min(-1) with PCA as substrate. kcat is 0.43 min(-1) with CA as substrate.1 Publication
- KM=0.030 mM for 5OMeBA1 Publication
- KM=0.15 mM for PCA1 Publication
- KM=0.49 mM for CA1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 66 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 74 | S-adenosyl-L-methioninePROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 92 | S-adenosyl-L-methioninePROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 121 | S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 139 | Divalent metal cationPROSITE-ProRule annotation1 Publication | 1 | |
Binding sitei | 141 | S-adenosyl-L-methioninePROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 165 | Divalent metal cationPROSITE-ProRule annotation1 Publication | 1 | |
Metal bindingi | 166 | Divalent metal cationPROSITE-ProRule annotation1 Publication | 1 |
GO - Molecular functioni
- catechol O-methyltransferase activity Source: UniProtKB-EC
- L-dopa O-methyltransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- orcinol O-methyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4306-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Catechol O-methyltransferase1 Publication (EC:2.1.1.61 Publication)Short name: COMT1 Publication |
Gene namesi | Ordered Locus Names:Rv0187Imported |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0187 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 142 | K → A: Retains a low but substantial activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000448281 | 1 – 220 | Catechol O-methyltransferaseAdd BLAST | 220 |
Proteomic databases
PaxDbi | O07431 |
PRIDEi | O07431 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv0187 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 68 – 69 | S-adenosyl-L-methionine bindingPROSITE-ProRule annotation1 Publication | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | ENOG4108VQJ Bacteria COG4122 LUCA |
HOGENOMi | HOG000016838 |
OMAi | MGEHPRL |
PhylomeDBi | O07431 |
Family and domain databases
InterProi | View protein in InterPro IPR029063 SAM-dependent_MTases IPR002935 SAM_O-MeTrfase |
Pfami | View protein in Pfam PF01596 Methyltransf_3, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51682 SAM_OMT_I, 1 hit |
i Sequence
Sequence statusi: Complete.
O07431-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGMDQQPNPP DVDAFLDSTL VGDDPALAAA LAASDAAELP RIAVSAQQGK
60 70 80 90 100
FLCLLAGAIQ ARRVLEIGTL GGFSTIWLAR GAGPQGRVVT LEYQPKHAEV
110 120 130 140 150
ARVNLQRAGV ADRVEVVVGP ALDTLPTLAG GPFDLVFIDA DKENNVAYIQ
160 170 180 190 200
WAIRLARRGA VIVVDNVIRG GGILAESDDA DAVAARRTLQ MMGEHPGLDA
210 220
TAIQTVGRKG WDGFALALVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP42914.1 |
RefSeqi | NP_214701.1, NC_000962.3 WP_003401129.1, NZ_NVQJ01000001.1 |
Genome annotation databases
EnsemblBacteriai | CCP42914; CCP42914; Rv0187 |
GeneIDi | 886779 |
KEGGi | mtu:Rv0187 mtv:RVBD_0187 |
PATRICi | fig|83332.111.peg.215 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP42914.1 |
RefSeqi | NP_214701.1, NC_000962.3 WP_003401129.1, NZ_NVQJ01000001.1 |
3D structure databases
SMRi | O07431 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0187 |
Proteomic databases
PaxDbi | O07431 |
PRIDEi | O07431 |
Genome annotation databases
EnsemblBacteriai | CCP42914; CCP42914; Rv0187 |
GeneIDi | 886779 |
KEGGi | mtu:Rv0187 mtv:RVBD_0187 |
PATRICi | fig|83332.111.peg.215 |
Organism-specific databases
TubercuListi | Rv0187 |
Phylogenomic databases
eggNOGi | ENOG4108VQJ Bacteria COG4122 LUCA |
HOGENOMi | HOG000016838 |
OMAi | MGEHPRL |
PhylomeDBi | O07431 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4306-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR029063 SAM-dependent_MTases IPR002935 SAM_O-MeTrfase |
Pfami | View protein in Pfam PF01596 Methyltransf_3, 1 hit |
SUPFAMi | SSF53335 SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51682 SAM_OMT_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAMT_MYCTU | |
Accessioni | O07431Primary (citable) accession number: O07431 Secondary accession number(s): F2GLY5, I6WXN4, L0T2S4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2019 |
Last sequence update: | July 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names