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UniProtKB - O07347 (SRP54_THEAQ)
Protein
Signal recognition particle protein
Gene
ffh
Organism
Thermus aquaticus
Status
Functioni
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
UniRule annotationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 105 – 112 | GTPUniRule annotation | 8 | |
Nucleotide bindingi | 187 – 191 | GTPUniRule annotation | 5 | |
Nucleotide bindingi | 245 – 248 | GTPUniRule annotation | 4 |
GO - Molecular functioni
- 7S RNA binding Source: InterPro
- GTPase activity Source: UniProtKB-UniRule
- GTP binding Source: UniProtKB-UniRule
GO - Biological processi
- SRP-dependent cotranslational protein targeting to membrane Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Signal recognition particle proteinUniRule annotationAlternative name(s): Fifty-four homologUniRule annotation |
Gene namesi | Name:ffhUniRule annotation |
Organismi | Thermus aquaticus |
Taxonomic identifieri | 271 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation
Other locations
- signal recognition particle Source: UniProtKB-UniRule
Keywords - Cellular componenti
Cytoplasm, Signal recognition particlePathology & Biotechi
Chemistry databases
DrugBanki | DB01942, Formic acid DB04315, Guanosine-5'-Diphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000101170 | 2 – 430 | Signal recognition particle proteinAdd BLAST | 429 |
Interactioni
Subunit structurei
Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.
UniRule annotationBinary interactionsi
O07347
With | #Exp. | IntAct |
---|---|---|
ftsY [P83749] | 3 | EBI-1037906,EBI-1037899 |
Protein-protein interaction databases
IntActi | O07347, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O07347 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O07347 |
Family & Domainsi
Domaini
Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation3 Publications
Sequence similaritiesi
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR004780, SRP IPR036225, SRP/SRP_N IPR022941, SRP54 IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit PTHR11564:SF7, PTHR11564:SF7, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47364, SSF47364, 1 hit SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00959, ffh, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O07347-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFQQLSARLQ EAIGRLRGRG RITEEDLKAT LREIRRALMD ADVNLEVARD
60 70 80 90 100
FVERVREEAL GKQVLESLTP AEVILATVYE ALKEALGGEA RLPVLKDRNL
110 120 130 140 150
WFLVGLQGSG KTTTAAKLAL YYKGKGRRPL LVAADTQRPA AREQLRLLGE
160 170 180 190 200
KVGVPVLEVM DGESPESIRR RVEEKARLEA RDLILVDTAG RLQIDEPLMG
210 220 230 240 250
ELARLKEVLG PDEVLLVLDA MTGQEALSVA RAFDEKVGVT GLVLTKLDGD
260 270 280 290 300
ARGGAALSAR HVTGKPIYFA GVSEKPEGLE PFYPERLAGR ILGMGDVASL
310 320 330 340 350
AEKVRAAGLE AEAPKSAKEL SLEDFLKQMQ NLKRLGPFSE ILGLLPGVPQ
360 370 380 390 400
GLKVDEKAIK RLEAIVLSMT PEERKDPRIL NGSRRKRIAK GSGTSVQEVN
410 420 430
RFIKAFEEMK ALMKSLEKKK GRGLMGMFRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82109 Genomic DNA Translation: AAB58502.1 |
RefSeqi | WP_003045728.1, NZ_LHCI01000106.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82109 Genomic DNA Translation: AAB58502.1 |
RefSeqi | WP_003045728.1, NZ_LHCI01000106.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FFH | X-ray | 2.05 | A | 2-295 | [»] | |
1JPJ | X-ray | 2.30 | A | 1-296 | [»] | |
1JPN | X-ray | 1.90 | A/B | 1-296 | [»] | |
1LS1 | X-ray | 1.10 | A | 1-295 | [»] | |
1NG1 | X-ray | 2.03 | A | 1-294 | [»] | |
1O87 | X-ray | 2.10 | A/B | 2-297 | [»] | |
1OKK | X-ray | 2.05 | A | 1-294 | [»] | |
1RJ9 | X-ray | 1.90 | B | 1-300 | [»] | |
1RY1 | electron microscopy | 12.00 | U | 1-296 | [»] | |
2C03 | X-ray | 1.24 | A/B | 2-297 | [»] | |
2C04 | X-ray | 1.15 | A/B | 2-297 | [»] | |
2CNW | X-ray | 2.39 | A/B/C | 2-294 | [»] | |
2FFH | X-ray | 3.20 | A/B/C | 1-425 | [»] | |
2IY3 | electron microscopy | 16.00 | A | 2-296 | [»] | |
2J45 | X-ray | 1.14 | A/B | 2-297 | [»] | |
2J46 | X-ray | 1.14 | A/B | 2-297 | [»] | |
2J7P | X-ray | 1.97 | A/B | 2-294 | [»] | |
2NG1 | X-ray | 2.02 | A | 2-294 | [»] | |
2XKV | electron microscopy | 13.50 | A | 1-294 | [»] | |
3NG1 | X-ray | 2.30 | A/B | 1-294 | [»] | |
3ZN8 | electron microscopy | 12.00 | A | 2-295 | [»] | |
SMRi | O07347 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O07347, 1 interactor |
Chemistry databases
DrugBanki | DB01942, Formic acid DB04315, Guanosine-5'-Diphosphate |
Miscellaneous databases
EvolutionaryTracei | O07347 |
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR004780, SRP IPR036225, SRP/SRP_N IPR022941, SRP54 IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit PTHR11564:SF7, PTHR11564:SF7, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47364, SSF47364, 1 hit SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00959, ffh, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SRP54_THEAQ | |
Accessioni | O07347Primary (citable) accession number: O07347 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families