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Protein

Maltose-6'-phosphate glucosidase

Gene

malH

Organism
Fusobacterium mortiferum
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including maltose-6'P, trehalose-6P and the 6'-phosphorylated derivatives of the five linkage-isomeric alpha-D-glucosyl-D-fructoses: trehalulose-6'P, turanose-6'P, maltulose-6'P, leucrose-6'P, and palatinose-6'P. However, sucrose-6P is not a substrate for MalH, and this enzyme also fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'P and gentobiose-6'P.

Catalytic activityi

Alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • NAD+
  • Mn2+, Fe2+, Co2+, Ni2+Note: Divalent metal ion. Manganese, iron, cobalt and nickel ions enhance activity whereas magnesium, zinc, calcium and strontium do not.

pH dependencei

Optimum pH is 7.0-7.5.

Temperature dependencei

Optimum temperature is 40 degrees Celsius.

Pathwayi: maltose degradation

This protein is involved in the pathway maltose degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway maltose degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateBy similarity1
Sitei109Increases basicity of active site TyrBy similarity1
Binding sitei147SubstrateBy similarity1
Metal bindingi169ManganeseBy similarity1
Active sitei170Proton donorBy similarity1
Metal bindingi200ManganeseBy similarity1
Active sitei264Proton acceptorBy similarity1
Binding sitei284SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 70NADBy similarityAdd BLAST67

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCobalt, Iron, Manganese, Metal-binding, NAD, Nickel

Enzyme and pathway databases

BRENDAi3.2.1.122 2369
SABIO-RKiO06901
UniPathwayi
UPA00150

Protein family/group databases

CAZyiGH4 Glycoside Hydrolase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Maltose-6'-phosphate glucosidase (EC:3.2.1.122)
Alternative name(s):
6-phospho-alpha-D-glucosidase
Gene namesi
Name:malH
OrganismiFusobacterium mortiferum
Taxonomic identifieri850 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698641 – 441Maltose-6'-phosphate glucosidaseAdd BLAST441

Expressioni

Inductioni

By the five sucrose isomers and other alpha-glucosides (but not by sucrose or glucose).

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO06901
SMRiO06901
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiENOG4105F8D Bacteria
COG1486 LUCA
KOiK01232

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR019802 GlycHydrolase_4_CS
IPR001088 Glyco_hydro_4
IPR022616 Glyco_hydro_4_C
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR32092 PTHR32092, 1 hit
PfamiView protein in Pfam
PF02056 Glyco_hydro_4, 1 hit
PF11975 Glyco_hydro_4C, 1 hit
PRINTSiPR00732 GLHYDRLASE4
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
PROSITEiView protein in PROSITE
PS01324 GLYCOSYL_HYDROL_F4, 1 hit

Sequencei

Sequence statusi: Complete.

O06901-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQFSILIAG GGSTFTPGII LMLLDNLDKF PIRQIKMFDN DAERQAKIGE
60 70 80 90 100
ACAILLKEKA PQIKFSYSTN PEEAFTDIDF VMAHIRVGKY PMRELDEKIP
110 120 130 140 150
LRHGVVGQET CGPGGIAYGM RSIGGVIGLI DYMEKYSPNA WMLNYSNPAA
160 170 180 190 200
IVAEATRRLR PNSKVLNICD MPIGIEVRMA EILGLESRKD MDIMYYGLNH
210 220 230 240 250
FGWWKSVRDK QGNDLMPKLR EHVSQYGYVV PKGDNQHTEA SWNDTFAKAK
260 270 280 290 300
DVLALDPTTL PNTYLKYYLF PDYVVEHSNK EYTRANEVMD GREKFVFGEC
310 320 330 340 350
EKVVKNQSSE GCALHIDEHA SYIVDLARAI AFNTKEKMLL IVENNGAIVN
360 370 380 390 400
FDSTAMVEIP CIVGSNGPEP LVVGRIPQFQ KGMMEQQVTV EKLTVEAWIE
410 420 430 440
GSYQKLWQAI TMSKTVPSAK VAKDILDDLI EANKEYWPVL K
Length:441
Mass (Da):49,712
Last modified:July 1, 1997 - v1
Checksum:iEE9D85B35FA8AF46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81185 Genomic DNA Translation: AAB63015.1

Genome annotation databases

KEGGiag:AAB63015

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81185 Genomic DNA Translation: AAB63015.1

3D structure databases

ProteinModelPortaliO06901
SMRiO06901
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH4 Glycoside Hydrolase Family 4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB63015

Phylogenomic databases

eggNOGiENOG4105F8D Bacteria
COG1486 LUCA
KOiK01232

Enzyme and pathway databases

UniPathwayi
UPA00150

BRENDAi3.2.1.122 2369
SABIO-RKiO06901

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR019802 GlycHydrolase_4_CS
IPR001088 Glyco_hydro_4
IPR022616 Glyco_hydro_4_C
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR32092 PTHR32092, 1 hit
PfamiView protein in Pfam
PF02056 Glyco_hydro_4, 1 hit
PF11975 Glyco_hydro_4C, 1 hit
PRINTSiPR00732 GLHYDRLASE4
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
PROSITEiView protein in PROSITE
PS01324 GLYCOSYL_HYDROL_F4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMALH_FUSMR
AccessioniPrimary (citable) accession number: O06901
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: February 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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