UniProtKB - O06553 (F420R_MYCTU)
Protein
F420H(2)-dependent reductase Rv1155
Gene
Rv1155
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
F420H2-dependent reductase able to catalyze the reduction of biliverdin-IXalpha to bilirubin-IXalpha in vitro. However, kinetic parameters show that it is less efficient than the biliverdin reductase Rv2074 and suggest biliverdin-IXalpha is unlikely to be the native substrate of Rv1155, which probably catalyzes the reduction of an alternative molecule in vivo (PubMed:27364382). Binds coenzyme F420, but does not bind FMN or other flavins (PubMed:25644473). Cannot use pyridoxine 5'-phosphate, pyridoxamine 5'-phosphate, pyridoxal 5'-phosphate (PLP), the anti-tuberculosis drug PA-824 or aflatoxin analogs as substrates (PubMed:25644473).2 Publications
Caution
Was originally thought to be an FMN-dependent pyridoxine 5'-phosphate oxidase.1 Publication
FMN and PLP are seen in the crystal structures published in PubMed:16239726, however, the enzyme does not bind these compounds in vitro, and the complexes obtained in the crystal structures may be the result of weak FMN/PLP binding that is stabilized by the crystal lattice.1 Publication
Kineticsi
kcat is 0.024 sec(-1) for the reduction of biliverdin.1 Publication
- KM=82.8 µM for biliverdin1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | Coenzyme F4201 Publication | 1 | |
Binding sitei | 37 | Coenzyme F420; via amide nitrogen1 Publication | 1 | |
Binding sitei | 50 | Coenzyme F420; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 138 | Coenzyme F420; shared with dimeric partner1 Publication | 1 |
GO - Molecular functioni
- coenzyme F420 binding Source: UniProtKB
- FMN binding Source: MTBBASE
- oxidoreductase activity, acting on the CH-CH group of donors Source: GO_Central
- protein homodimerization activity Source: UniProtKB
- pyridoxal phosphate binding Source: MTBBASE
GO - Biological processi
- vitamin B6 metabolic process Source: MTBBASE
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BRENDAi | 1.4.3.5, 3445 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Rv1155 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1155 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000399901 | 1 – 147 | F420H(2)-dependent reductase Rv1155Add BLAST | 147 |
Proteomic databases
PaxDbi | O06553 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 83332.Rv1155 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O06553 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O06553 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 60 | Coenzyme F420-binding1 Publication | 5 | |
Regioni | 77 – 79 | Coenzyme F420-binding; shared with dimeric partner1 Publication | 3 |
Sequence similaritiesi
Belongs to the F420H(2)-dependent biliverdin reductase family.Curated
Phylogenomic databases
eggNOGi | COG3467, Bacteria |
InParanoidi | O06553 |
OMAi | DPRASYH |
PhylomeDBi | O06553 |
Family and domain databases
Gene3Di | 2.30.110.10, 1 hit |
InterProi | View protein in InterPro IPR019920, F420-binding_dom_put IPR011576, Pyridox_Oxase_put IPR012349, Split_barrel_FMN-bd |
Pfami | View protein in Pfam PF01243, Putative_PNPOx, 1 hit |
TIGRFAMsi | TIGR03618, Rv1155_F420, 1 hit |
i Sequence
Sequence statusi: Complete.
O06553-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARQVFDDKL LAVISGNSIG VLATIKHDGR PQLSNVQYHF DPRKLLIQVS
60 70 80 90 100
IAEPRAKTRN LRRDPRASIL VDADDGWSYA VAEGTAQLTP PAAAPDDDTV
110 120 130 140
EALIALYRNI AGEHSDWDDY RQAMVTDRRV LLTLPISHVY GLPPGMR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43910.1 |
PIRi | D70555 |
RefSeqi | NP_215671.1, NC_000962.3 WP_003898745.1, NZ_NVQJ01000025.1 |
Genome annotation databases
EnsemblBacteriai | CCP43910; CCP43910; Rv1155 |
GeneIDi | 23492700 885604 |
KEGGi | mtu:Rv1155 mtv:RVBD_1155 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43910.1 |
PIRi | D70555 |
RefSeqi | NP_215671.1, NC_000962.3 WP_003898745.1, NZ_NVQJ01000025.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1W9A | X-ray | 1.80 | A/B | 1-147 | [»] | |
1XXO | X-ray | 1.80 | A/B | 1-147 | [»] | |
1Y30 | X-ray | 2.20 | A/B | 1-147 | [»] | |
2AQ6 | X-ray | 1.70 | A/B | 1-147 | [»] | |
4QVB | X-ray | 2.30 | A/B | 2-147 | [»] | |
SMRi | O06553 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1155 |
Proteomic databases
PaxDbi | O06553 |
Genome annotation databases
EnsemblBacteriai | CCP43910; CCP43910; Rv1155 |
GeneIDi | 23492700 885604 |
KEGGi | mtu:Rv1155 mtv:RVBD_1155 |
Organism-specific databases
TubercuListi | Rv1155 |
Phylogenomic databases
eggNOGi | COG3467, Bacteria |
InParanoidi | O06553 |
OMAi | DPRASYH |
PhylomeDBi | O06553 |
Enzyme and pathway databases
BRENDAi | 1.4.3.5, 3445 |
Miscellaneous databases
EvolutionaryTracei | O06553 |
Family and domain databases
Gene3Di | 2.30.110.10, 1 hit |
InterProi | View protein in InterPro IPR019920, F420-binding_dom_put IPR011576, Pyridox_Oxase_put IPR012349, Split_barrel_FMN-bd |
Pfami | View protein in Pfam PF01243, Putative_PNPOx, 1 hit |
TIGRFAMsi | TIGR03618, Rv1155_F420, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F420R_MYCTU | |
Accessioni | O06553Primary (citable) accession number: O06553 Secondary accession number(s): L0T7G6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2010 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families