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UniProtKB - O06491 (GATA_BACSU)
Protein
Glutamyl-tRNA(Gln) amidotransferase subunit A
Gene
gatA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphateEC:6.3.5.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 79 | Charge relay systemBy similarity | 1 | |
Active sitei | 154 | Charge relay systemBy similarity | 1 | |
Active sitei | 178 | Acyl-ester intermediateBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU06680-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit A (EC:6.3.5.7)Short name: Glu-ADT subunit A |
Gene namesi | Name:gatA Synonyms:yedB, yerM Ordered Locus Names:BSU06680 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105138 | 1 – 485 | Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST | 485 |
Proteomic databases
jPOSTi | O06491 |
PaxDbi | O06491 |
PRIDEi | O06491 |
Interactioni
Subunit structurei
Heterotrimer of A, B and C subunits.
Protein-protein interaction databases
IntActi | O06491, 1 interactor |
MINTi | O06491 |
STRINGi | 224308.BSU06680 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0154, Bacteria |
InParanoidi | O06491 |
OMAi | MYLSDIF |
PhylomeDBi | O06491 |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
i Sequence
Sequence statusi: Complete.
O06491-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLFDHKITE LKQLIHKKEI KISDLVDESY KRIQAVDDKV QAFLALDEER
60 70 80 90 100
ARAYAKELDE AVDGRSEHGL LFGMPIGVKD NIVTKGLRTT CSSKILENFD
110 120 130 140 150
PIYDATVVQR LQDAEAVTIG KLNMDEFAMG SSTENSAYKL TKNPWNLDTV
160 170 180 190 200
PGGSSGGSAA AVAAGEVPFS LGSDTGGSIR QPASFCGVVG LKPTYGRVSR
210 220 230 240 250
YGLVAFASSL DQIGPITRTV EDNAFLLQAI SGVDKMDSTS ANVDVPDFLS
260 270 280 290 300
SLTGDIKGLK IAVPKEYLGE GVGKEARESV LAALKVLEGL GATWEEVSLP
310 320 330 340 350
HSKYALATYY LLSSSEASAN LARFDGIRYG YRTDNADNLI DLYKQTRAEG
360 370 380 390 400
FGNEVKRRIM LGTFALSSGY YDAYYKKAQK VRTLIKKDFE DVFEKYDVIV
410 420 430 440 450
GPTTPTPAFK IGENTKDPLT MYANDILTIP VNLAGVPGIS VPCGLADGLP
460 470 480
LGLQIIGKHF DESTVYRVAH AFEQATDHHK AKPEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 435 – 444 | GVPGISVPCG → AYRESGAMR in AAB83964 (PubMed:9342321).Curated | 10 | |
Sequence conflicti | 463 – 485 | STVYR…AKPEL → ALYTALLMHLNKQQTIIKQN LNCKG in AAB83964 (PubMed:9342321).CuratedAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008553 Genomic DNA Translation: AAB83964.1 AL009126 Genomic DNA Translation: CAB12488.1 AF011545 Genomic DNA Translation: AAB72184.1 U92466 Genomic DNA Translation: AAB66513.1 |
PIRi | B69795 T44452 T51582 |
RefSeqi | NP_388550.1, NC_000964.3 WP_003242878.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12488; CAB12488; BSU_06680 |
GeneIDi | 938748 |
KEGGi | bsu:BSU06680 |
PATRICi | fig|224308.179.peg.726 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008553 Genomic DNA Translation: AAB83964.1 AL009126 Genomic DNA Translation: CAB12488.1 AF011545 Genomic DNA Translation: AAB72184.1 U92466 Genomic DNA Translation: AAB66513.1 |
PIRi | B69795 T44452 T51582 |
RefSeqi | NP_388550.1, NC_000964.3 WP_003242878.1, NZ_JNCM01000032.1 |
3D structure databases
SMRi | O06491 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O06491, 1 interactor |
MINTi | O06491 |
STRINGi | 224308.BSU06680 |
Proteomic databases
jPOSTi | O06491 |
PaxDbi | O06491 |
PRIDEi | O06491 |
Genome annotation databases
EnsemblBacteriai | CAB12488; CAB12488; BSU_06680 |
GeneIDi | 938748 |
KEGGi | bsu:BSU06680 |
PATRICi | fig|224308.179.peg.726 |
Phylogenomic databases
eggNOGi | COG0154, Bacteria |
InParanoidi | O06491 |
OMAi | MYLSDIF |
PhylomeDBi | O06491 |
Enzyme and pathway databases
BioCyci | BSUB:BSU06680-MONOMER |
Miscellaneous databases
PROi | PR:O06491 |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GATA_BACSU | |
Accessioni | O06491Primary (citable) accession number: O06491 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families