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UniProtKB - O05701 (DHPS_STAAU)
Protein
Dihydropteroate synthase
Gene
folP
Organism
Staphylococcus aureus
Status
Functioni
Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate, the immediate precursor of folate derivatives.
1 PublicationMiscellaneous
Specific mutations in this gene are a major cause of sulfonamide resistance in MRSA clinical isolates.1 Publication
Catalytic activityi
- (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate1 PublicationEC:2.5.1.151 Publication
Cofactori
Mg2+By similarity1 PublicationNote: Magnesium is required for activity, even if it seems to interact primarily with the substrate.Curated
Kineticsi
- KM=5.4 µM for 4-aminobenzoate1 Publication
- KM=9.3 µM for 6-hydroxymethyl-7,8-dihydropterin diphosphate1 Publication
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.1 Publication This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 11 | Magnesium1 Publication | 1 | |
Binding sitei | 52 | 6-hydroxymethyl-7,8-dihydropterin diphosphateCombined sources1 Publication | 1 | |
Binding sitei | 84 | 6-hydroxymethyl-7,8-dihydropterin diphosphateCombined sources1 Publication | 1 | |
Binding sitei | 103 | 6-hydroxymethyl-7,8-dihydropterin diphosphateCombined sources1 Publication | 1 | |
Binding sitei | 167 | 6-hydroxymethyl-7,8-dihydropterin diphosphateCombined sources1 Publication | 1 | |
Binding sitei | 203 | 6-hydroxymethyl-7,8-dihydropterin diphosphateCombined sources1 Publication | 1 |
GO - Molecular functioni
- dihydropteroate synthase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- folic acid biosynthetic process Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
- tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Antibiotic resistance, Folate biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.5.1.15, 3352 |
SABIO-RKi | O05701 |
UniPathwayi | UPA00077;UER00156 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydropteroate synthase1 Publication (EC:2.5.1.151 Publication)Short name: DHPS1 Publication Alternative name(s): Dihydropteroate pyrophosphorylase |
Gene namesi | Name:folP Synonyms:dpsA1 Publication |
Organismi | Staphylococcus aureus |
Taxonomic identifieri | 1280 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168225 | 1 – 267 | Dihydropteroate synthaseAdd BLAST | 267 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O05701 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O05701 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 251 | Pterin-bindingPROSITE-ProRule annotationAdd BLAST | 251 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 239 – 241 | 6-hydroxymethyl-7,8-dihydropterin diphosphate bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the DHPS family.Curated
Family and domain databases
CDDi | cd00739, DHPS, 1 hit |
Gene3Di | 3.20.20.20, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF00809, Pterin_bind, 1 hit |
SUPFAMi | SSF51717, SSF51717, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit |
PROSITEi | View protein in PROSITE PS00792, DHPS_1, 1 hit PS00793, DHPS_2, 1 hit PS50972, PTERIN_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
O05701-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKTKIMGIL NVTPDSFSDG GKFNNVESAV TRVKAMMDEG ADIIDVGGVS
60 70 80 90 100
TRPGHEMITV EEELNRVLPV VEAIVGFDVK ISVDTFRSEV AEACLKLGVD
110 120 130 140 150
IINDQWAGLY DHRMFQVVAK YDAEIVLMHN GNGNRDEPVV EEMLTSLLAQ
160 170 180 190 200
AHQAKIAGIP SNKIWLDPGI GFAKTRNEEA EVMARLDELV ATEYPVLLAT
210 220 230 240 250
SRKRFTKEMM GYDTTPVERD EVTAATTAYG IMKGVRAVRV HNVELNAKLA
260
KGIDFLKENE NARHNFS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z84573 Genomic DNA Translation: CAB06539.1 |
RefSeqi | WP_000167936.1, NZ_WKIW01000023.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z84573 Genomic DNA Translation: CAB06539.1 |
RefSeqi | WP_000167936.1, NZ_WKIW01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AD1 | X-ray | 2.20 | A/B | 2-267 | [»] | |
1AD4 | X-ray | 2.40 | A/B | 2-267 | [»] | |
6CLU | X-ray | 1.95 | A/B/C/D | 1-267 | [»] | |
6CLV | X-ray | 2.30 | A/B/C/D | 1-267 | [»] | |
SMRi | O05701 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00156 |
BRENDAi | 2.5.1.15, 3352 |
SABIO-RKi | O05701 |
Miscellaneous databases
EvolutionaryTracei | O05701 |
Family and domain databases
CDDi | cd00739, DHPS, 1 hit |
Gene3Di | 3.20.20.20, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF00809, Pterin_bind, 1 hit |
SUPFAMi | SSF51717, SSF51717, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit |
PROSITEi | View protein in PROSITE PS00792, DHPS_1, 1 hit PS00793, DHPS_2, 1 hit PS50972, PTERIN_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHPS_STAAU | |
Accessioni | O05701Primary (citable) accession number: O05701 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families