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UniProtKB - O05516 (TSAB_BACSU)
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
Gene
tsaB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE; this reaction does not require ATP in vitro. TsaB seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD.
1 PublicationMiscellaneous
The four proteins YwlC, TsaD, TsaB and TsaE are necessary and sufficient for tRNA(NNU) t6A37 threonylcarbamoyladenosine biosynthesis in vitro in B.subtilis.1 Publication
Caution
The well-known t6A modification appears to be a hydrolyzed artifact of natural cyclic t6A (ct6A) that occurs during the preparation and handling of tRNA in B.subtilis and many other species (PubMed:23242255). In these species, the t6A modification is processed further by dehydration into ct6A, a reaction catalyzed by TcdA.Curated
GO - Biological processi
- tRNA threonylcarbamoyladenosine modification Source: UniProtKB
Keywordsi
Biological process | tRNA processing |
Enzyme and pathway databases
BioCyci | BSUB:BSU05920-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA threonylcarbamoyladenosine biosynthesis protein TsaBAlternative name(s): t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB |
Gene namesi | Name:tsaB Synonyms:ydiC Ordered Locus Names:BSU05920 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Cells lacking this gene display a slow growth phenotype. Cell size, shape and nucleoid morphology remain unchanged.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000387461 | 1 – 229 | tRNA threonylcarbamoyladenosine biosynthesis protein TsaBAdd BLAST | 229 |
Proteomic databases
PaxDbi | O05516 |
Interactioni
Subunit structurei
Forms a heterodimer with TsaE.
1 PublicationProtein-protein interaction databases
STRINGi | 224308.BSU05920 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1214, Bacteria |
InParanoidi | O05516 |
OMAi | RRMEVYS |
PhylomeDBi | O05516 |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR022496, T6A_TsaB |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF11, PTHR11735:SF11, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR03725, T6A_YeaZ, 1 hit |
i Sequence
Sequence statusi: Complete.
O05516-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTILAIDTSN YTLGIALLRE DTVIAEYITY LKKNHSVRAM PAVHSLLNDC
60 70 80 90 100
DMAPQDLSKI VVAKGPGSYT GVRIGVTLAK TLAWSLDIPI SAVSSLETLA
110 120 130 140 150
ANGRHFDGLI SPIFDARRGQ VYTGLYQYKN GLLEQVVPDQ NVMLADWLEM
160 170 180 190 200
LKEKDRPVLF LGHDTSLHKQ MIEDVLGTKG FIGTAAQHNP RPSELAFLGK
210 220
EKEAADVHGL VPDYLRLAEA EAKWIESQK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88802 Genomic DNA Translation: BAA19716.1 AL009126 Genomic DNA Translation: CAB12411.1 |
PIRi | D69786 |
RefSeqi | NP_388473.1, NC_000964.3 WP_003234078.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12411; CAB12411; BSU_05920 |
GeneIDi | 939874 |
KEGGi | bsu:BSU05920 |
PATRICi | fig|224308.179.peg.637 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D88802 Genomic DNA Translation: BAA19716.1 AL009126 Genomic DNA Translation: CAB12411.1 |
PIRi | D69786 |
RefSeqi | NP_388473.1, NC_000964.3 WP_003234078.1, NZ_JNCM01000032.1 |
3D structure databases
SMRi | O05516 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU05920 |
Proteomic databases
PaxDbi | O05516 |
Genome annotation databases
EnsemblBacteriai | CAB12411; CAB12411; BSU_05920 |
GeneIDi | 939874 |
KEGGi | bsu:BSU05920 |
PATRICi | fig|224308.179.peg.637 |
Phylogenomic databases
eggNOGi | COG1214, Bacteria |
InParanoidi | O05516 |
OMAi | RRMEVYS |
PhylomeDBi | O05516 |
Enzyme and pathway databases
BioCyci | BSUB:BSU05920-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR043129, ATPase_NBD IPR000905, Gcp-like_dom IPR022496, T6A_TsaB |
PANTHERi | PTHR11735, PTHR11735, 1 hit PTHR11735:SF11, PTHR11735:SF11, 1 hit |
Pfami | View protein in Pfam PF00814, TsaD, 1 hit |
SUPFAMi | SSF53067, SSF53067, 2 hits |
TIGRFAMsi | TIGR03725, T6A_YeaZ, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TSAB_BACSU | |
Accessioni | O05516Primary (citable) accession number: O05516 Secondary accession number(s): Q797D5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 3, 2009 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families