We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - O05268 (FENR2_BACSU)
Protein
Ferredoxin--NADP reductase 2
Gene
yumC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]1 PublicationEC:1.18.1.21 Publication
Cofactori
FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication
Kineticsi
KM for NADH is < 0.1 µM.
- KM=0.54 µM for NADPH (at 24 degrees Celsius and pH 7.0)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | FADBy similarity | 1 | |
Binding sitei | 45 | FADBy similarity | 1 | |
Binding sitei | 50 | FADBy similarity | 1 | |
Binding sitei | 90 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 124 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 285 | FADBy similarity | 1 | |
Binding sitei | 326 | FADBy similarity | 1 |
GO - Molecular functioni
- ferredoxin-NADP+ reductase activity Source: UniProtKB-UniRule
- flavin adenine dinucleotide binding Source: UniProtKB-UniRule
- NADP binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BioCyci | BSUB:BSU32110-MONOMER |
BRENDAi | 1.14.14.47, 9933 1.18.1.2, 658 1.19.1.1, 658 |
SABIO-RKi | O05268 |
Names & Taxonomyi
Protein namesi | Recommended name: Ferredoxin--NADP reductase 2 (EC:1.18.1.2)Short name: FNR 2 Short name: Fd-NADP(+) reductase 2 |
Gene namesi | Name:yumC Ordered Locus Names:BSU32110 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000360548 | 1 – 332 | Ferredoxin--NADP reductase 2Add BLAST | 332 |
Proteomic databases
jPOSTi | O05268 |
PaxDbi | O05268 |
PRIDEi | O05268 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
IntActi | O05268, 1 interactor |
MINTi | O05268 |
STRINGi | 224308.BSU32110 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O05268 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O05268 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ferredoxin--NADP reductase type 2 family.Curated
Phylogenomic databases
eggNOGi | COG0492, Bacteria |
InParanoidi | O05268 |
OMAi | PEKVIYD |
PhylomeDBi | O05268 |
Family and domain databases
Gene3Di | 3.50.50.60, 2 hits |
HAMAPi | MF_01685, FENR2, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR022890, Fd--NADP_Rdtase_type_2 |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
i Sequence
Sequence statusi: Complete.
O05268-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MREDTKVYDI TIIGGGPVGL FTAFYGGMRQ ASVKIIESLP QLGGQLSALY
60 70 80 90 100
PEKYIYDVAG FPKIRAQELI NNLKEQMAKF DQTICLEQAV ESVEKQADGV
110 120 130 140 150
FKLVTNEETH YSKTVIITAG NGAFKPRKLE LENAEQYEGK NLHYFVDDLQ
160 170 180 190 200
KFAGRRVAIL GGGDSAVDWA LMLEPIAKEV SIIHRRDKFR AHEHSVENLH
210 220 230 240 250
ASKVNVLTPF VPAELIGEDK IEQLVLEEVK GDRKEILEID DLIVNYGFVS
260 270 280 290 300
SLGPIKNWGL DIEKNSIVVK STMETNIEGF FAAGDICTYE GKVNLIASGF
310 320 330
GEAPTAVNNA KAYMDPKARV QPLHSTSLFE NK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 105 – 116 | TNEET…SKTVI → QMKKPTTLKRSC in CAB07954 (Ref. 1) CuratedAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z93939 Genomic DNA Translation: CAB07954.1 AL009126 Genomic DNA Translation: CAB15201.2 |
PIRi | B70015 |
RefSeqi | NP_391091.2, NC_000964.3 WP_003220618.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15201; CAB15201; BSU_32110 |
GeneIDi | 64304956 936577 |
KEGGi | bsu:BSU32110 |
PATRICi | fig|224308.179.peg.3477 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z93939 Genomic DNA Translation: CAB07954.1 AL009126 Genomic DNA Translation: CAB15201.2 |
PIRi | B70015 |
RefSeqi | NP_391091.2, NC_000964.3 WP_003220618.1, NZ_JNCM01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3LZW | X-ray | 1.80 | A | 1-332 | [»] | |
3LZX | X-ray | 1.90 | A/B | 1-332 | [»] | |
SMRi | O05268 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O05268, 1 interactor |
MINTi | O05268 |
STRINGi | 224308.BSU32110 |
Proteomic databases
jPOSTi | O05268 |
PaxDbi | O05268 |
PRIDEi | O05268 |
Genome annotation databases
EnsemblBacteriai | CAB15201; CAB15201; BSU_32110 |
GeneIDi | 64304956 936577 |
KEGGi | bsu:BSU32110 |
PATRICi | fig|224308.179.peg.3477 |
Phylogenomic databases
eggNOGi | COG0492, Bacteria |
InParanoidi | O05268 |
OMAi | PEKVIYD |
PhylomeDBi | O05268 |
Enzyme and pathway databases
BioCyci | BSUB:BSU32110-MONOMER |
BRENDAi | 1.14.14.47, 9933 1.18.1.2, 658 1.19.1.1, 658 |
SABIO-RKi | O05268 |
Miscellaneous databases
EvolutionaryTracei | O05268 |
PROi | PR:O05268 |
Family and domain databases
Gene3Di | 3.50.50.60, 2 hits |
HAMAPi | MF_01685, FENR2, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR022890, Fd--NADP_Rdtase_type_2 |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FENR2_BACSU | |
Accessioni | O05268Primary (citable) accession number: O05268 Secondary accession number(s): Q795K8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2009 |
Last sequence update: | July 28, 2009 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families