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UniProtKB - O05207 (TOP6B_SACSH)
Protein
Type 2 DNA topoisomerase 6 subunit B
Gene
top6B
Organism
Saccharolobus shibatae (Sulfolobus shibatae)
Status
Functioni
Relaxes both positive and negative supercoils and exhibits a strong decatenase and unknotting activity; it cannot introduce DNA supercoils (PubMed:7961685).
ATP is absolutely required for DNA cleavage; the nonhydrolyzable analog AMP-PNP generates nicked or linear products from a supercoiled dsDNA substrate. Generates staggered two-nucleotide long 5' overhangs. The enzyme is covalently attached transiently to the 5'-ends of the cleaved strands (PubMed:11485995).
2 PublicationsCatalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation1 Publication EC:5.6.2.2
Activity regulationi
Not inhibited by the DNA gyrase inhibitor novobiocin, instead inhibited by eukaryotic topoisomerase inhibitors such as m- and o-amsacrine, ellipticine, and the quinolone CP-115,953 (PubMed:7961685). Radicicol inhibits the ATPase activity (PubMed:16920739).2 Publications
Temperature dependencei
Optimum temperature is 70-80 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | ATPCombined sources2 Publications | 1 | |
Binding sitei | 76 | ATPCombined sources2 Publications | 1 | |
Binding sitei | 427 | ATPCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 96 – 98 | ATPCombined sources2 Publications | 3 | |
Nucleotide bindingi | 107 – 113 | ATPCombined sources2 Publications | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB-UniRule
- identical protein binding Source: IntAct
GO - Biological processi
- DNA topological change Source: UniProtKB-UniRule
- DNA unwinding involved in DNA replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 5.6.2.2, 6162 |
Names & Taxonomyi
Protein namesi | Recommended name: Type 2 DNA topoisomerase 6 subunit BUniRule annotation (EC:5.6.2.2UniRule annotation1 Publication)Alternative name(s): Type II DNA topoisomerase VI subunit BUniRule annotation Short name: TopoVI-BUniRule annotation |
Gene namesi | Name:top6BUniRule annotation |
Organismi | Saccharolobus shibatae (Sulfolobus shibatae) |
Taxonomic identifieri | 2286 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus |
Subcellular locationi
Other locations
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145470 | 1 – 530 | Type 2 DNA topoisomerase 6 subunit BAdd BLAST | 530 |
Interactioni
Subunit structurei
Homodimer. Heterotetramer of two Top6A and two Top6B chains.
UniRule annotation6 PublicationsBinary interactionsi
O05207
With | #Exp. | IntAct |
---|---|---|
top6A [O05208] | 5 | EBI-9026762,EBI-6430603 |
itself | 3 | EBI-9026762,EBI-9026762 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-29416N |
IntActi | O05207, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O05207 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O05207 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TOP6B family.UniRule annotation
Family and domain databases
CDDi | cd00823, TopoIIB_Trans, 1 hit |
Gene3Di | 3.30.230.10, 1 hit 3.30.565.10, 1 hit |
HAMAPi | MF_00322, Top6B, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR010979, Ribosomal_S13-like_H2TH IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005734, TopoVI_B IPR015320, TopoVI_B_transducer |
PANTHERi | PTHR10871:SF4, PTHR10871:SF4, 1 hit |
Pfami | View protein in Pfam PF09239, Topo-VIb_trans, 1 hit |
PIRSFi | PIRSF006553, TopoVI_B, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF46946, SSF46946, 1 hit SSF54211, SSF54211, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01052, top6b, 1 hit |
i Sequence
Sequence statusi: Complete.
O05207-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAKEKFTSL SPAEFFKRNP ELAGFPNPAR ALYQTVRELI ENSLDATDVH
60 70 80 90 100
GILPNIKITI DLIDDARQIY KVNVVDNGIG IPPQEVPNAF GRVLYSSKYV
110 120 130 140 150
NRQTRGMYGL GVKAAVLYSQ MHQDKPIEIE TSPVNSKRIY TFKLKIDINK
160 170 180 190 200
NEPIIVERGS VENTRGFHGT SVAISIPGDW PKAKSRIYEY IKRTYIITPY
210 220 230 240 250
AEFIFKDPEG NVTYYPRLTN KIPKPPQEVK PHPYGVDREE IKILINNLKR
260 270 280 290 300
DYTIKEFLVN EFQSIGDTTA DKILELAGLK PNKKVKNLTE EEITRLVETF
310 320 330 340 350
KKDEDFRSPS ADSLSVIGED LIELGLKKIF NPDFAASITR KPKAYQGHPF
360 370 380 390 400
IVEAGVAFGG SIPVGEEPIV LRYANKIPLI YDEKSDVIWK VVEELDWKRY
410 420 430 440 450
GIESDQYQMV VMVHLCSTKI PYKSAGKESI AEVENIEKEI KNALMEVARK
460 470 480 490 500
LKQYLSEKRK EQEAKKKLLA YLKYIPEVSR SLATFLASGN KELVSKYQNE
510 520 530
ISEGLFKLIS KKLDLINIEE YRKVYRVDSE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10582 Genomic DNA Translation: CAA71604.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10582 Genomic DNA Translation: CAA71604.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MU5 | X-ray | 2.00 | A | 2-470 | [»] | |
1MX0 | X-ray | 2.30 | A/B/C/D/E/F | 1-470 | [»] | |
1Z59 | X-ray | 2.10 | A | 2-470 | [»] | |
1Z5A | X-ray | 2.20 | A/B | 2-470 | [»] | |
1Z5B | X-ray | 2.00 | A/B | 2-470 | [»] | |
1Z5C | X-ray | 2.20 | A/B | 2-470 | [»] | |
2HKJ | X-ray | 2.00 | A | 2-470 | [»] | |
2ZBK | X-ray | 3.56 | B/D/F/H | 1-530 | [»] | |
SMRi | O05207 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29416N |
IntActi | O05207, 1 interactor |
Enzyme and pathway databases
BRENDAi | 5.6.2.2, 6162 |
Miscellaneous databases
EvolutionaryTracei | O05207 |
Family and domain databases
CDDi | cd00823, TopoIIB_Trans, 1 hit |
Gene3Di | 3.30.230.10, 1 hit 3.30.565.10, 1 hit |
HAMAPi | MF_00322, Top6B, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR010979, Ribosomal_S13-like_H2TH IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005734, TopoVI_B IPR015320, TopoVI_B_transducer |
PANTHERi | PTHR10871:SF4, PTHR10871:SF4, 1 hit |
Pfami | View protein in Pfam PF09239, Topo-VIb_trans, 1 hit |
PIRSFi | PIRSF006553, TopoVI_B, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF46946, SSF46946, 1 hit SSF54211, SSF54211, 1 hit SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR01052, top6b, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TOP6B_SACSH | |
Accessioni | O05207Primary (citable) accession number: O05207 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families