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Protein

Pyruvate kinase

Gene

pyk

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), Phosphoglyceromutase (MexAM1_META1p1219), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35SubstrateBy similarity1
Metal bindingi37PotassiumBy similarity1
Metal bindingi39PotassiumBy similarity1
Metal bindingi69PotassiumBy similarity1
Sitei217Transition state stabilizerBy similarity1
Metal bindingi219MagnesiumBy similarity1
Binding sitei242Substrate; via amide nitrogenBy similarity1
Metal bindingi243MagnesiumBy similarity1
Binding sitei243Substrate; via amide nitrogenBy similarity1
Binding sitei275SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:MexAM1_META1p2941
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120781 – 478Pyruvate kinaseAdd BLAST478

Proteomic databases

PRIDEiO05118

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p2941

Structurei

3D structure databases

ProteinModelPortaliO05118
SMRiO05118
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
HOGENOMiHOG000021558
KOiK00873
OMAiGSHDDIR

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

O05118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSRRTKIV ATLGPASDTP EMIEKLFHAG ADVFRINMSH LAREKLPERI
60 70 80 90 100
EVIRTIEREA KRPIGILVDL QGPKLRLGTF VGDAAVLENG QTFVLDSDPT
110 120 130 140 150
PGDTDRVFLP HPEILSALEP SHGILIDDGK LRLIVTEVSE GRAVTRVEVG
160 170 180 190 200
GRISNRKGVS LPHTVLPVPA MTEKDRGDLE AGLAAGADWI AVSFVQRPED
210 220 230 240 250
VAEVKKVAAG RALVMAKIEK PQALTRLDEI IEISDGIMVA RGDLGVEMPL
260 270 280 290 300
EQVPGVQKRI TRVARRLGKP VVVATQMLES MITSPVPTRA EVSDVATAVY
310 320 330 340 350
EGADAVMLSA ESAAGDFPVE AIGTMNRIAE QVERDALYWS ILMAQRSEPE
360 370 380 390 400
PTASDAIAAA AHQIVEALSL RSIMAWTHSG STVLRLARAR PNASVIALTP
410 420 430 440 450
KRETARRLTM AWGVHPIVTK DASDVDDMAF RAAKFAVRER FAEIGDRVII
460 470
VAGVPFGIPG ATNMVRIAFV TREHAERA
Length:478
Mass (Da):51,801
Last modified:September 22, 2009 - v2
Checksum:i6C58142F1B17E3AA
GO

Sequence cautioni

The sequence AAB66498 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60A → G in AAB66498 (Ref. 1) Curated1
Sequence conflicti165V → A in AAB66498 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87316 Genomic DNA Translation: AAB66498.1 Different initiation.
CP001510 Genomic DNA Translation: ACS40693.1
RefSeqiWP_003601864.1, NC_012808.1

Genome annotation databases

EnsemblBacteriaiACS40693; ACS40693; MexAM1_META1p2941
KEGGimea:Mex_1p2941

Similar proteinsi

Entry informationi

Entry nameiKPYK_METEA
AccessioniPrimary (citable) accession number: O05118
Secondary accession number(s): C5AUT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 22, 2009
Last modified: May 23, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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