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Protein

Folate synthesis bifunctional protein, mitochondrial

Gene

MitHPPK/DHPS

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis.1 Publication

Catalytic activityi

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate.1 Publication
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.1 Publication

Cofactori

Mg2+By similarity

Kineticsi

  1. KM=0.7 µM for 6-hydroxymethyl-7,8-dihydropterin1 Publication
  2. KM=70 µM for ATP1 Publication
  3. KM=30 µM for 6-hydroxymethyl-7,8-dihydropterin-pyrophosphate1 Publication
  4. KM=0.6 µM for p-aminobenzoic acid1 Publication

    pH dependencei

    Optimum pH is 9.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Pathwayi: tetrahydrofolate biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.Curated
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. no protein annotated in this organism
    3. no protein annotated in this organism
    4. Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
    This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

    Pathwayi: tetrahydrofolate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.Curated
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Folate synthesis bifunctional protein, mitochondrial (MitHPPK/DHPS)
    2. no protein annotated in this organism
    This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi237MagnesiumBy similarity1
    Binding sitei2776-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei3146-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei3336-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei4066-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
    Binding sitei4516-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

    GO - Molecular functioni

    • 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: UniProtKB
    • ATP binding Source: UniProtKB-KW
    • dihydropteroate synthase activity Source: UniProtKB
    • kinase activity Source: UniProtKB-KW
    • magnesium ion binding Source: UniProtKB

    GO - Biological processi

    • folic acid biosynthetic process Source: UniProtKB
    • tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionKinase, Multifunctional enzyme, Transferase
    Biological processFolate biosynthesis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00155
    UPA00077; UER00156

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Folate synthesis bifunctional protein, mitochondrial1 Publication
    Including the following 2 domains:
    6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase1 Publication (EC:2.7.6.31 Publication)
    Short name:
    HPPK1 Publication
    Alternative name(s):
    2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
    7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
    Short name:
    PPPK
    Dihydropteroate synthase1 Publication (EC:2.5.1.151 Publication)
    Short name:
    DHPS1 Publication
    Gene namesi
    Name:MitHPPK/DHPS1 Publication
    OrganismiPisum sativum (Garden pea)Imported
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 28Mitochondrion1 PublicationAdd BLAST28
    ChainiPRO_500014695729 – 515Folate synthesis bifunctional protein, mitochondrialAdd BLAST487

    Expressioni

    Inductioni

    Not induced by light.1 Publication

    Interactioni

    Subunit structurei

    Homomultimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO04862
    SMRiO04862
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini230 – 498Pterin-bindingPROSITE-ProRule annotationAdd BLAST269

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni47 – 172HPPKCuratedAdd BLAST126
    Regioni232 – 515DHPSAdd BLAST284
    Regioni486 – 4886-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

    Sequence similaritiesi

    In the N-terminal section; belongs to the HPPK family.Curated
    In the C-terminal section; belongs to the DHPS family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    CDDicd00739 DHPS, 1 hit
    cd00483 HPPK, 1 hit
    Gene3Di3.20.20.20, 1 hit
    3.30.70.560, 1 hit
    InterProiView protein in InterPro
    IPR006390 DHP_synth
    IPR011005 Dihydropteroate_synth-like
    IPR000550 Hppk
    IPR035907 Hppk_sf
    IPR000489 Pterin-binding_dom
    PfamiView protein in Pfam
    PF01288 HPPK, 1 hit
    PF00809 Pterin_bind, 1 hit
    SUPFAMiSSF51717 SSF51717, 1 hit
    SSF55083 SSF55083, 1 hit
    TIGRFAMsiTIGR01496 DHPS, 1 hit
    TIGR01498 folK, 1 hit
    PROSITEiView protein in PROSITE
    PS00792 DHPS_1, 1 hit
    PS00793 DHPS_2, 1 hit
    PS00794 HPPK, 1 hit
    PS50972 PTERIN_BINDING, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O04862-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSILKCLGVR GNQLCAARNY LKVLGFSSFH TAPNSSIEIQ TQDEEVVIAL
    60 70 80 90 100
    GSNVGDRLHN FKEALKLMRK SGIHITRHAS LYETAPAYVT DQPRFLNSAV
    110 120 130 140 150
    RADTKLGPHE LLAALKRIEK DMGRTDGIRY GPRPIDLDIL FYGKFKVRSD
    160 170 180 190 200
    ILTVPHERIW ERPFVMAPLM DLLGTAIDSD TVASWHSFSG HSGGLNALWE
    210 220 230 240 250
    KLGGESLIGE EGMYRVMPVA NGLLDWSRRT LVMGILNLTP DSFSDGGNFQ
    260 270 280 290 300
    SVKSAVSQAR LMISEGADII DIGAQSTRPM ASRISAEEEL GRLIPVLEAV
    310 320 330 340 350
    MSIPEVEGKL ISVDTFYSEV ALEAVRKGAH IINDVSAGKL DASMFKVMAE
    360 370 380 390 400
    LDVPYVAMHM RGDPSTMQDS ENLKYDNVCK DISSELYSRV REAEISGIPA
    410 420 430 440 450
    WRIIMDPGIG FSKKTEDNLA ALTGIPDIRE EISKRSLAIS HAPILIGPSR
    460 470 480 490 500
    KRFLGEICSR PSAVDRDPAT IASVTAGVLC GANIVRVHNV KDNLDAVKLC
    510
    DAILKQKSSP IKFKQ
    Length:515
    Mass (Da):56,455
    Last modified:July 1, 1997 - v1
    Checksum:iE6D03CB710CCB56A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y08611 mRNA Translation: CAA69903.1
    PIRiT06595

    Similar proteinsi

    Entry informationi

    Entry nameiFOLM_PEA
    AccessioniPrimary (citable) accession number: O04862
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
    Last sequence update: July 1, 1997
    Last modified: May 23, 2018
    This is version 80 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

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