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UniProtKB - O04200 (PXN_ARATH)
Protein
Peroxisomal nicotinamide adenine dinucleotide carrier
Gene
PXN
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Mediates the NAD+ import into peroxisomes. Favors the NAD+(in)/AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport that might be essential under special conditions. Transports CoA, dephospho-CoA, acetyl-CoA, adenosine 3',5'-diphosphate (PAP), NAD+, AMP, ADP and NADH, but has no activity with ATP, GTP, GDP, NADPH, NADP+ or FAD. Required for peroxisomes proliferation.
3 PublicationsCaution
Was originally thought to be an adenine nucleotide carrier.1 Publication
Activity regulationi
Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride and bromocresol purple.1 Publication
Kineticsi
- KM=102 µM for the NAD+/NAD+ exchange2 Publications
- KM=119 µM for the AMP/AMP exchange2 Publications
- Vmax=166 µmol/min/g enzyme for the NAD+/NAD+ exchange2 Publications
- Vmax=416 µmol/min/g enzyme for the AMP/AMP exchange2 Publications
GO - Molecular functioni
- antiporter activity Source: UniProtKB-KW
GO - Biological processi
- NAD transport Source: TAIR
- regulation of peroxisome size Source: TAIR
Keywordsi
Biological process | Antiport, Transport |
Protein family/group databases
TCDBi | 2.A.29.6.2, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal nicotinamide adenine dinucleotide carrierAlternative name(s): Peroxisomal NAD carrier Peroxisomal membrane protein 38, (PMP36) Short name: AtPMP38 Protein ABERRANT PEROXISOME MORPHOLOGY 3 Solute carrier family 25 member 17 |
Gene namesi | Name:PXN Synonyms:APEM3 Ordered Locus Names:At2g39970 ORF Names:T28M21.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G39970 |
TAIRi | locus:2061176, AT2G39970 |
Subcellular locationi
Peroxisome
- Glyoxysome membrane 3 Publications; Multi-pass membrane protein 3 Publications
Peroxisome
- glyoxysomal membrane Source: UniProtKB-SubCell
- peroxisome Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 5 – 25 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 63 – 85 | Helical; Name=2Sequence analysisAdd BLAST | 23 | |
Transmembranei | 116 – 136 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 180 – 200 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 235 – 255 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 293 – 313 | Helical; Name=6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Glyoxysome, Membrane, PeroxisomePathology & Biotechi
Disruption phenotypei
No germination or growth inhibition, but delayed storage oil mobilization. Decreased sensitivity to 4-(2,4-dichlorophenoxy)butanoic acid (2,4-DB), a precursor converted in vivo by beta-oxidation into the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Enlarged peroxisomes.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420694 | 1 – 331 | Peroxisomal nicotinamide adenine dinucleotide carrierAdd BLAST | 331 |
Proteomic databases
PaxDbi | O04200 |
ProMEXi | O04200 |
ProteomicsDBi | 226132 |
PTM databases
iPTMneti | O04200 |
Expressioni
Tissue specificityi
Expressed in cotyledons, hypocotyls, vascular tissues, trichomes, hydathodes, seeds, pedicels, flowers and stigma.2 Publications
Gene expression databases
ExpressionAtlasi | O04200, baseline and differential |
Genevisiblei | O04200, AT |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 3922, 1 interactor |
STRINGi | 3702.AT2G39970.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 2 – 91 | Solcar 1Add BLAST | 90 | |
Repeati | 109 – 216 | Solcar 2Add BLAST | 108 | |
Repeati | 229 – 320 | Solcar 3Add BLAST | 92 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0769, Eukaryota |
HOGENOMi | CLU_015166_6_3_1 |
InParanoidi | O04200 |
OMAi | YEWTRSF |
OrthoDBi | 1186395at2759 |
PhylomeDBi | O04200 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR044712, SLC25A32-like |
PANTHERi | PTHR45683, PTHR45683, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
O04200-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDALINGLA GAGGGIIAQL LTYPLQTVNT RQQTERDLKR EKRKLGTIEH
60 70 80 90 100
MCQVVKQEGW ERLYGGLAPS LAGTAASQGV YYYFYQVFRN RAEATALARK
110 120 130 140 150
KKGLGDGSVG MFASLLVAAF AGSVNVLMTN PIWVIVTRMQ THRKMTKDQT
160 170 180 190 200
AAPESPSSNA EALVAVEPRP YGTFNTIREV YDEAGITGFW KGVIPTLIMV
210 220 230 240 250
SNPSMQFMLY ETMLTKLKKK RALKGSNNVT ALETFLLGAV AKLGATVTTY
260 270 280 290 300
PLLVVKSRLQ AKQVTTGDKR QQYKGTLDAI LKMIRYEGLY GFYKGMSTKI
310 320 330
VQSVLAAAVL FMIKEELVKG AKLLLSNATS S
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 331 | S → I in AAM65850 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB047148 mRNA Translation: BAB62814.1 AF002109 Genomic DNA Translation: AAB95282.1 CP002685 Genomic DNA Translation: AEC09757.1 AY039858 mRNA Translation: AAK63962.1 BT003020 mRNA Translation: AAO23585.1 AY088311 mRNA Translation: AAM65850.1 |
PIRi | F84823 |
RefSeqi | NP_181526.1, NM_129555.4 |
Genome annotation databases
EnsemblPlantsi | AT2G39970.1; AT2G39970.1; AT2G39970 |
GeneIDi | 818584 |
Gramenei | AT2G39970.1; AT2G39970.1; AT2G39970 |
KEGGi | ath:AT2G39970 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB047148 mRNA Translation: BAB62814.1 AF002109 Genomic DNA Translation: AAB95282.1 CP002685 Genomic DNA Translation: AEC09757.1 AY039858 mRNA Translation: AAK63962.1 BT003020 mRNA Translation: AAO23585.1 AY088311 mRNA Translation: AAM65850.1 |
PIRi | F84823 |
RefSeqi | NP_181526.1, NM_129555.4 |
3D structure databases
AlphaFoldDBi | O04200 |
SMRi | O04200 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 3922, 1 interactor |
STRINGi | 3702.AT2G39970.1 |
Protein family/group databases
TCDBi | 2.A.29.6.2, the mitochondrial carrier (mc) family |
PTM databases
iPTMneti | O04200 |
Proteomic databases
PaxDbi | O04200 |
ProMEXi | O04200 |
ProteomicsDBi | 226132 |
Genome annotation databases
EnsemblPlantsi | AT2G39970.1; AT2G39970.1; AT2G39970 |
GeneIDi | 818584 |
Gramenei | AT2G39970.1; AT2G39970.1; AT2G39970 |
KEGGi | ath:AT2G39970 |
Organism-specific databases
Araporti | AT2G39970 |
TAIRi | locus:2061176, AT2G39970 |
Phylogenomic databases
eggNOGi | KOG0769, Eukaryota |
HOGENOMi | CLU_015166_6_3_1 |
InParanoidi | O04200 |
OMAi | YEWTRSF |
OrthoDBi | 1186395at2759 |
PhylomeDBi | O04200 |
Miscellaneous databases
PROi | PR:O04200 |
Gene expression databases
ExpressionAtlasi | O04200, baseline and differential |
Genevisiblei | O04200, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR044712, SLC25A32-like |
PANTHERi | PTHR45683, PTHR45683, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PXN_ARATH | |
Accessioni | O04200Primary (citable) accession number: O04200 Secondary accession number(s): Q8L9P5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2013 |
Last sequence update: | July 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families