UniProtKB - O04005 (REHY_ARATH)
Protein
1-Cys peroxiredoxin PER1
Gene
PER1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (By similarity). Seems to contribute to the inhibition of germination during stress (PubMed:14526116).By similarity1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.By similarity
Catalytic activityi
- EC:1.11.1.24By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 46 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- thioredoxin peroxidase activity Source: TAIR
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- maintenance of seed dormancy Source: TAIR
- response to desiccation Source: TAIR
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | ARA:AT1G48130-MONOMER |
Protein family/group databases
PeroxiBasei | 4362, At1CysPrx |
Names & Taxonomyi
Protein namesi | Recommended name: 1-Cys peroxiredoxin PER1 (EC:1.11.1.24By similarity)Alternative name(s): Rehydrin homolog Thioredoxin peroxidase Thioredoxin-dependent peroxiredoxinCurated |
Gene namesi | Name:PER1 Ordered Locus Names:At1g48130 ORF Names:F21D18.15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G48130 |
TAIRi | locus:2023772, AT1G48130 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135107 | 1 – 216 | 1-Cys peroxiredoxin PER1Add BLAST | 216 |
Proteomic databases
PaxDbi | O04005 |
PRIDEi | O04005 |
ProteomicsDBi | 234913 |
Expressioni
Tissue specificityi
Predominantly expressed in seed. Expressed in endosperm, embryo and aleurone cells. Also detected in young seedlings, abscission zones, stem branching points.2 Publications
Developmental stagei
Expressed during the late globular stage and late torpedo stage of the embryo, and in distinct cells of unfertilized and fertilized ovules.1 Publication
Inductioni
By abscisic acid (ABA) and oxidative stress.1 Publication
Gene expression databases
ExpressionAtlasi | O04005, baseline and differential |
Genevisiblei | O04005, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 159 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 156 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 191 – 214 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG0854, Eukaryota |
HOGENOMi | CLU_042529_4_1_1 |
OMAi | IPLTCRA |
OrthoDBi | 1129256at2759 |
PhylomeDBi | O04005 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O04005-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGITLGDTV PNLEVETTHD KFKLHDYFAN SWTVLFSHPG DFTPVCTTEL
60 70 80 90 100
GAMAKYAHEF DKRGVKLLGL SCDDVQSHKD WIKDIEAFNH GSKVNYPIIA
110 120 130 140 150
DPNKEIIPQL NMIDPIENGP SRALHIVGPD SKIKLSFLYP STTGRNMDEV
160 170 180 190 200
LRALDSLLMA SKHNNKIATP VNWKPDQPVV ISPAVSDEEA KKMFPQGFKT
210
ADLPSKKGYL RHTEVS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 23 | K → T in CAA85539 (Ref. 6) Curated | 1 | |
Sequence conflicti | 77 | S → T in CAA85539 (Ref. 6) Curated | 1 | |
Sequence conflicti | 107 | I → N in CAA85539 (Ref. 6) Curated | 1 | |
Sequence conflicti | 212 | H → R in AAT41856 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 212 | H → R in AAO41963 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12089 Genomic DNA Translation: CAA72804.1 AC023673 Genomic DNA Translation: AAF79536.1 CP002684 Genomic DNA Translation: AEE32252.1 BT003916 mRNA Translation: AAO41963.1 BT014873 mRNA Translation: AAT41856.1 Z37278 mRNA Translation: CAA85539.1 |
RefSeqi | NP_175247.1, NM_103709.4 |
Genome annotation databases
EnsemblPlantsi | AT1G48130.1; AT1G48130.1; AT1G48130 |
GeneIDi | 841231 |
Gramenei | AT1G48130.1; AT1G48130.1; AT1G48130 |
KEGGi | ath:AT1G48130 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12089 Genomic DNA Translation: CAA72804.1 AC023673 Genomic DNA Translation: AAF79536.1 CP002684 Genomic DNA Translation: AEE32252.1 BT003916 mRNA Translation: AAO41963.1 BT014873 mRNA Translation: AAT41856.1 Z37278 mRNA Translation: CAA85539.1 |
RefSeqi | NP_175247.1, NM_103709.4 |
3D structure databases
SMRi | O04005 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G48130.1 |
Protein family/group databases
PeroxiBasei | 4362, At1CysPrx |
Proteomic databases
PaxDbi | O04005 |
PRIDEi | O04005 |
ProteomicsDBi | 234913 |
Genome annotation databases
EnsemblPlantsi | AT1G48130.1; AT1G48130.1; AT1G48130 |
GeneIDi | 841231 |
Gramenei | AT1G48130.1; AT1G48130.1; AT1G48130 |
KEGGi | ath:AT1G48130 |
Organism-specific databases
Araporti | AT1G48130 |
TAIRi | locus:2023772, AT1G48130 |
Phylogenomic databases
eggNOGi | KOG0854, Eukaryota |
HOGENOMi | CLU_042529_4_1_1 |
OMAi | IPLTCRA |
OrthoDBi | 1129256at2759 |
PhylomeDBi | O04005 |
Enzyme and pathway databases
BioCyci | ARA:AT1G48130-MONOMER |
Miscellaneous databases
PROi | PR:O04005 |
Gene expression databases
ExpressionAtlasi | O04005, baseline and differential |
Genevisiblei | O04005, AT |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | REHY_ARATH | |
Accessioni | O04005Primary (citable) accession number: O04005 Secondary accession number(s): Q42319, Q84WE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families