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Protein

Phosphatidylinositol 4-kinase beta

Gene

PI4KB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).By similarity1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.1 Publication

Activity regulationi

Inhibited by wortmannin. Increased kinase activity upon interaction with NCS1/FREQ.2 Publications

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB
  • 1-phosphatidylinositol 4-kinase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase beta (EC:2.7.1.67)
Short name:
PI4K-beta
Short name:
PI4Kbeta
Short name:
PtdIns 4-kinase beta
Gene namesi
Name:PI4KB
Synonyms:PIK4CB
OrganismiBos taurus (Bovine)Imported
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000888282 – 816Phosphatidylinositol 4-kinase betaAdd BLAST815

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei263PhosphothreonineBy similarity1
Modified residuei266PhosphoserineBy similarity1
Modified residuei275PhosphoserineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei284PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei428PhosphoserineBy similarity1
Modified residuei438PhosphothreonineBy similarity1
Modified residuei511PhosphoserineBy similarity1
Modified residuei517PhosphothreonineBy similarity1
Modified residuei519PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO02810
PRIDEiO02810

Interactioni

Subunit structurei

Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (By similarity). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (PubMed:11526106). Interacts with ACBD3. Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009627

Structurei

Secondary structure

1816
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO02810
SMRiO02810
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 242PIK helicalPROSITE-ProRule annotationAdd BLAST214
Domaini543 – 750PI3K/PI4KPROSITE-ProRule annotationCuratedAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 68Interaction with ACBD3By similarityAdd BLAST67

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0903 Eukaryota
ENOG410XPH3 LUCA
HOGENOMiHOG000008289
HOVERGENiHBG053597
InParanoidiO02810
KOiK19801

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 2 hits
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
SMARTiView protein in SMART
SM00146 PI3Kc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 11 Publication (identifier: O02810-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGDTIVEPAP LKPTSEPAPG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK
60 70 80 90 100
ACQEVLEKVK LLHGGVAISS RGTPLELVNG DGVDSEIRCL DDPPAQIREE
110 120 130 140 150
EDEMGATVAS GTAKGARRRR QNNSAKQSWL LRLFESKLFD ISMAISYLYN
160 170 180 190 200
SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL NMYIHMDEDV GDAIKPYIVH
210 220 230 240 250
RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI LSDELKPAHR
260 270 280 290 300
KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN
310 320 330 340 350
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ
360 370 380 390 400
RLISELSLLN HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY
410 420 430 440 450
VEVLECENFD TTSVPARIPE NRIRSTRSVE NLPECGITHE QRAGSFSTVP
460 470 480 490 500
NYDNDDEAWS VDDIGELQVE LPEVHTNSCD NISQFSVDSI TSQESKEPVF
510 520 530 540 550
IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK VRRIREGSPY
560 570 580 590 600
GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI
610 620 630 640 650
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ
660 670 680 690 700
RNFVQSCAGY CLGCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN
710 720 730 740 750
LGFETSAFKL TTEFVDVMGG LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI
760 770 780 790 800
VEIMQQGSQL PCFHGSSTIR NLKERFHMSM TEEQLQLLVE QMVDGSMRSI
810
TTKLYDGFQY LTNGIM
Length:816
Mass (Da):91,375
Last modified:November 14, 2003 - v2
Checksum:i4388D127636B500A
GO
Isoform 21 Publication (identifier: O02810-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Show »
Length:801
Mass (Da):89,803
Checksum:i8B6B0D34450ED08C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050628304 – 318Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88531 mRNA Translation: AAC48729.1
RefSeqiNP_777208.1, NM_174783.2 [O02810-2]
UniGeneiBt.688

Genome annotation databases

GeneIDi286846
KEGGibta:286846

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88531 mRNA Translation: AAC48729.1
RefSeqiNP_777208.1, NM_174783.2 [O02810-2]
UniGeneiBt.688

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EUQX-ray3.20E288-422[»]
ProteinModelPortaliO02810
SMRiO02810
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009627

Proteomic databases

PaxDbiO02810
PRIDEiO02810

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi286846
KEGGibta:286846

Organism-specific databases

CTDi5298

Phylogenomic databases

eggNOGiKOG0903 Eukaryota
ENOG410XPH3 LUCA
HOGENOMiHOG000008289
HOVERGENiHBG053597
InParanoidiO02810
KOiK19801

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 2 hits
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
SMARTiView protein in SMART
SM00146 PI3Kc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPI4KB_BOVIN
AccessioniPrimary (citable) accession number: O02810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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