UniProtKB - O02744 (IL12B_FELCA)
Interleukin-12 subunit beta
IL12B
Functioni
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
By similarityAssociates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- cytokine binding Source: GO_Central
- cytokine receptor activity Source: GO_Central
- interleukin-12 alpha subunit binding Source: GO_Central
GO - Biological processi
- cytokine-mediated signaling pathway Source: GO_Central
Keywordsi
Molecular function | Cytokine |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin-12 subunit betaShort name: IL-12B Alternative name(s): Cytotoxic lymphocyte maturation factor 40 kDa subunit Short name: CLMF p40 IL-12 subunit p40 |
Gene namesi | Name:IL12B |
Organismi | Felis catus (Cat) (Felis silvestris catus) |
Taxonomic identifieri | 9685 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- interleukin-12 complex Source: GO_Central
- interleukin-23 complex Source: GO_Central
Plasma Membrane
- external side of plasma membrane Source: GO_Central
Other locations
- receptor complex Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | By similarityAdd BLAST | 22 | |
ChainiPRO_0000010928 | 23 – 329 | Interleukin-12 subunit betaAdd BLAST | 307 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 50 ↔ 90 | PROSITE-ProRule annotation | ||
Glycosylationi | 135 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 200 | Interchain (with C-99 in IL12A)PROSITE-ProRule annotation | ||
Glycosylationi | 223 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: UniProtKB-KW
- cytokine binding Source: GO_Central
- interleukin-12 alpha subunit binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 9685.ENSFCAP00000014439 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 106 | Ig-like C2-typeAdd BLAST | 84 | |
Domaini | 238 – 329 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 92 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, SignalPhylogenomic databases
eggNOGi | ENOG502RZMA, Eukaryota |
InParanoidi | O02744 |
OrthoDBi | 1179405at2759 |
TreeFami | TF334829 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 3 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR003530, Hematopoietin_rcpt_L_F3_CS IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003598, Ig_sub2 IPR015528, IL-12_beta IPR019482, IL-12_beta_cen-dom |
PANTHERi | PTHR23036:SF156, PTHR23036:SF156, 1 hit |
Pfami | View protein in Pfam PF10420, IL12p40_C, 1 hit |
PIRSFi | PIRSF038007, IL_12_beta, 1 hit |
PRINTSi | PR01928, INTRLEUKN12B |
SMARTi | View protein in SMART SM00408, IGc2, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF49265, SSF49265, 2 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS01354, HEMATOPO_REC_L_F3, 1 hit PS50835, IG_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MHPQQLVIAW LSLVLLAPPL MAIWELEKNV YVVELDWHPD APGEMVVLTC
60 70 80 90 100
NTPEEDDITW TSDQSSEVLG SGKTLTIQVK EFADAGQYTC HKGGEVLSHS
110 120 130 140 150
FLLIHKKEDG IWSTDILREQ KESKNKIFLK CEAKNYSGRF TCWWLTAIST
160 170 180 190 200
DLKFTVKSSR GSSDPQEVTC GAATLSAEKV RVDNRDYKKY TVECQEGSAC
210 220 230 240 250
PAAEESLPIE VVVDAIHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS
260 270 280 290 300
RHVEVSWEYP DTWSTPHSYF SLTFGVQVQG KNNREKKDRL SVDKTSAKVV
310 320
CHKDAKIRVQ ARDRYYSSSW SNWASVSCS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | L → F in AAB93835 (PubMed:9522125).Curated | 1 | |
Sequence conflicti | 51 | N → D in AAB93835 (PubMed:9522125).Curated | 1 | |
Sequence conflicti | 167 | E → G in AAB93835 (PubMed:9522125).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07762 mRNA Translation: CAA69068.1 U83184 mRNA Translation: AAB93835.1 |
RefSeqi | NP_001070881.1, NM_001077413.1 |
Genome annotation databases
GeneIDi | 768273 |
KEGGi | fca:768273 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y07762 mRNA Translation: CAA69068.1 U83184 mRNA Translation: AAB93835.1 |
RefSeqi | NP_001070881.1, NM_001077413.1 |
3D structure databases
SMRi | O02744 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9685.ENSFCAP00000014439 |
Genome annotation databases
GeneIDi | 768273 |
KEGGi | fca:768273 |
Organism-specific databases
CTDi | 3593 |
Phylogenomic databases
eggNOGi | ENOG502RZMA, Eukaryota |
InParanoidi | O02744 |
OrthoDBi | 1179405at2759 |
TreeFami | TF334829 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 3 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR003530, Hematopoietin_rcpt_L_F3_CS IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003598, Ig_sub2 IPR015528, IL-12_beta IPR019482, IL-12_beta_cen-dom |
PANTHERi | PTHR23036:SF156, PTHR23036:SF156, 1 hit |
Pfami | View protein in Pfam PF10420, IL12p40_C, 1 hit |
PIRSFi | PIRSF038007, IL_12_beta, 1 hit |
PRINTSi | PR01928, INTRLEUKN12B |
SMARTi | View protein in SMART SM00408, IGc2, 1 hit |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF49265, SSF49265, 2 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS01354, HEMATOPO_REC_L_F3, 1 hit PS50835, IG_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IL12B_FELCA | |
Accessioni | O02744Primary (citable) accession number: O02744 Secondary accession number(s): O46671 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families