UniProtKB - O02703 (BAX_BOVIN)
Apoptosis regulator BAX
BAX
Functioni
GO - Molecular functioni
- BH3 domain binding Source: UniProtKB
- channel activity Source: UniProtKB
- lipid binding Source: UniProtKB
- protein heterodimerization activity Source: GO_Central
- protein homodimerization activity Source: HGNC
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c Source: UniProtKB
- apoptotic mitochondrial changes Source: UniProtKB
- apoptotic signaling pathway Source: UniProtKB
- B cell apoptotic process Source: UniProtKB
- establishment or maintenance of transmembrane electrochemical gradient Source: UniProtKB
- extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
- intrinsic apoptotic signaling pathway Source: UniProtKB
- intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
- mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
- mitochondrial fusion Source: UniProtKB
- negative regulation of mitochondrial membrane potential Source: UniProtKB
- negative regulation of protein binding Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of endoplasmic reticulum unfolded protein response Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of neuron apoptotic process Source: UniProtKB
- positive regulation of protein-containing complex assembly Source: UniProtKB
- positive regulation of release of cytochrome c from mitochondria Source: UniProtKB
- regulation of apoptotic process Source: UniProtKB
- regulation of mitochondrial membrane potential Source: UniProtKB
- release of cytochrome c from mitochondria Source: UniProtKB
- release of matrix enzymes from mitochondria Source: UniProtKB
- response to toxic substance Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Names & Taxonomyi
Protein namesi | Recommended name: Apoptosis regulator BAX |
Gene namesi | Name:BAX |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:26428, BAX |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Mitochondrion
- Mitochondrion outer membrane By similarity; Single-pass membrane protein Sequence analysis
Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane (By similarity).By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
- endoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrial outer membrane Source: UniProtKB
- mitochondrial permeability transition pore complex Source: UniProtKB
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- BAX complex Source: UniProtKB
- Bcl-2 family protein complex Source: UniProtKB
- cytoplasm Source: UniProtKB
- pore complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 172 – 192 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000143052 | 1 – 192 | Apoptosis regulator BAXAdd BLAST | 192 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | O02703 |
PeptideAtlasi | O02703 |
PRIDEi | O02703 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000013340, Expressed in placenta and 19 other tissues |
ExpressionAtlasi | O02703, baseline and differential |
Interactioni
Subunit structurei
Homodimer. Forms higher oligomers under stress conditions. Forms heterooligomers with BAK.
Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1.
Interacts with SH3GLB1 and HN.
Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria.
Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX.
Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells (By similarity).
Interacts with FAIM2/LFG2 (By similarity).
Interacts with BOP (By similarity).
Interacts with GIMAP3/IAN4 and GIMAP5/IAN5; this interaction is increased, when cells initiate apoptosis upon IL2 withdrawal (By similarity).
Interacts with IRF3; the interaction is direct and, upon virus infection, mediates the formation of the apoptosis complex TOMM70:HSP90AA1:IRF3:BAX (By similarity).
By similarityGO - Molecular functioni
- BH3 domain binding Source: UniProtKB
- protein heterodimerization activity Source: GO_Central
- protein homodimerization activity Source: HGNC
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000017739 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 59 – 73 | BH3Add BLAST | 15 | |
Motifi | 98 – 118 | BH1Add BLAST | 21 | |
Motifi | 150 – 165 | BH2Add BLAST | 16 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01020000230347 |
HOGENOMi | CLU_085401_2_2_1 |
InParanoidi | O02703 |
OMAi | RDHVINW |
OrthoDBi | 1218929at2759 |
TreeFami | TF315834 |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR026304, BAX IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR020726, Bcl2_BH2_motif_CS IPR020728, Bcl2_BH3_motif_CS IPR036834, Blc2-like_sf IPR026298, Blc2_fam |
PANTHERi | PTHR11256, PTHR11256, 1 hit PTHR11256:SF42, PTHR11256:SF42, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit |
PRINTSi | PR01862, BCL2FAMILY |
SMARTi | View protein in SMART SM00337, BCL, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS01258, BH2, 1 hit PS01259, BH3, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG ETPELGLEQV
60 70 80 90 100
PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAAEMF
110 120 130 140 150
SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG
160 170 180 190
WIQDQGGWDG LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1MN40 | A0A3Q1MN40_BOVIN | Apoptosis regulator BAX | BAX | 336 | Annotation score: | ||
A0A3Q1MT88 | A0A3Q1MT88_BOVIN | Apoptosis regulator BAX | BAX | 355 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92569 mRNA Translation: AAC48806.1 |
RefSeqi | NP_776319.1, NM_173894.1 [O02703-1] |
Genome annotation databases
Ensembli | ENSBTAT00000017739; ENSBTAP00000017739; ENSBTAG00000013340 [O02703-1] |
GeneIDi | 280730 |
KEGGi | bta:280730 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92569 mRNA Translation: AAC48806.1 |
RefSeqi | NP_776319.1, NM_173894.1 [O02703-1] |
3D structure databases
BMRBi | O02703 |
SMRi | O02703 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000017739 |
Proteomic databases
PaxDbi | O02703 |
PeptideAtlasi | O02703 |
PRIDEi | O02703 |
Genome annotation databases
Ensembli | ENSBTAT00000017739; ENSBTAP00000017739; ENSBTAG00000013340 [O02703-1] |
GeneIDi | 280730 |
KEGGi | bta:280730 |
Organism-specific databases
CTDi | 581 |
VGNCi | VGNC:26428, BAX |
Phylogenomic databases
eggNOGi | KOG4728, Eukaryota |
GeneTreei | ENSGT01020000230347 |
HOGENOMi | CLU_085401_2_2_1 |
InParanoidi | O02703 |
OMAi | RDHVINW |
OrthoDBi | 1218929at2759 |
TreeFami | TF315834 |
Gene expression databases
Bgeei | ENSBTAG00000013340, Expressed in placenta and 19 other tissues |
ExpressionAtlasi | O02703, baseline and differential |
Family and domain databases
CDDi | cd06845, Bcl-2_like, 1 hit |
Gene3Di | 1.10.437.10, 1 hit |
InterProi | View protein in InterPro IPR026304, BAX IPR002475, Bcl2-like IPR020717, Bcl2_BH1_motif_CS IPR020726, Bcl2_BH2_motif_CS IPR020728, Bcl2_BH3_motif_CS IPR036834, Blc2-like_sf IPR026298, Blc2_fam |
PANTHERi | PTHR11256, PTHR11256, 1 hit PTHR11256:SF42, PTHR11256:SF42, 1 hit |
Pfami | View protein in Pfam PF00452, Bcl-2, 1 hit |
PRINTSi | PR01862, BCL2FAMILY |
SMARTi | View protein in SMART SM00337, BCL, 1 hit |
SUPFAMi | SSF56854, SSF56854, 1 hit |
PROSITEi | View protein in PROSITE PS50062, BCL2_FAMILY, 1 hit PS01080, BH1, 1 hit PS01258, BH2, 1 hit PS01259, BH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAX_BOVIN | |
Accessioni | O02703Primary (citable) accession number: O02703 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families