UniProtKB - O02626 (MADD_CAEEL)
MAP kinase-activating death domain protein
aex-3
Functioni
Guanyl-nucleotide exchange factor that regulates small GTPases (By similarity).
Converts GDP-bound inactive form of rab-3 and cab-1 to the GTP-bound active forms. Regulator of presynaptic activity that interacts with rab-3 to regulate synaptic vesicle release (PubMed:9136770, PubMed:10970871).
Is also a regulator of the cab-1 synaptic transmission pathway (PubMed:10970871).
Probably by converting rab-3 to its GTP-bound active form, plays a role in the recruitment of endophilin unc-57 to synaptic vesicles (PubMed:21029864).
Probably by activating rab-3 and thus regulating the trafficking of dense-core vesicles, plays a role in AVG neuron-mediated formation of the right axon tract of the ventral nerve cord (PubMed:27116976).
Regulates anterior body muscle contractions (aBOC) and the expulsion steps during the defecation motor program (DMP) (PubMed:18852466).
Probably by regulating DMP, required for fatty acid uptake by intestinal cells (PubMed:25849533).
By similarity6 PublicationsGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: GO_Central
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- chemical synaptic transmission Source: WormBase
- defecation Source: WormBase
- lipid transport involved in lipid storage Source: UniProtKB
- mating Source: WormBase
- oviposition Source: WormBase
- positive regulation of acetylcholine secretion, neurotransmission Source: WormBase
- positive regulation of anterior/posterior axon guidance Source: UniProtKB
- positive regulation of defecation Source: UniProtKB
- positive regulation of intestinal lipid absorption Source: UniProtKB
- regulation of apoptotic process Source: GO_Central
- regulation of Rab protein signal transduction Source: GO_Central
- ventral cord development Source: UniProtKB
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-CEL-8876198, RAB GEFs exchange GTP for GDP on RABs |
Names & Taxonomyi
Protein namesi | Recommended name: MAP kinase-activating death domain proteinAlternative name(s): Aboc, expulsion defective protein 3 Regulator of presynaptic activity aex-3 |
Gene namesi | Name:aex-3 ORF Names:C02H7.3 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C02H7.3a ; CE16806 ; WBGene00000086 ; aex-3 C02H7.3b ; CE30850 ; WBGene00000086 ; aex-3 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- synapse Source: GOC
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064469 | 1 – 1409 | MAP kinase-activating death domain proteinAdd BLAST | 1409 |
Proteomic databases
EPDi | O02626 |
PaxDbi | O02626 |
PeptideAtlasi | O02626 |
PRIDEi | O02626 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | WBGene00000086, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
Interactioni
Subunit structurei
Interacts with cab-1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 45373, 1 interactor |
STRINGi | 6239.C02H7.3a |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 230 | uDENNPROSITE-ProRule annotationAdd BLAST | 205 | |
Domaini | 251 – 390 | cDENNPROSITE-ProRule annotationAdd BLAST | 140 | |
Domaini | 392 – 496 | dDENNPROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 1109 – 1184 | DeathAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 654 – 701 | DisorderedSequence analysisAdd BLAST | 48 | |
Regioni | 761 – 784 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 902 – 1008 | DisorderedSequence analysisAdd BLAST | 107 | |
Regioni | 1015 – 1034 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 654 – 692 | Polar residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 902 – 916 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 936 – 959 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 962 – 1005 | Pro residuesSequence analysisAdd BLAST | 44 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3570, Eukaryota |
GeneTreei | ENSGT00940000156718 |
HOGENOMi | CLU_001270_0_0_1 |
InParanoidi | O02626 |
OMAi | ETFSMSK |
OrthoDBi | 162625at2759 |
PhylomeDBi | O02626 |
Family and domain databases
Gene3Di | 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR039980, MADD IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
PANTHERi | PTHR13008, PTHR13008, 1 hit |
Pfami | View protein in Pfam PF02141, DENN, 1 hit PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00800, uDENN, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNDKEKEICP RLIDFLVVVG KRNRTRGASQ SSPDATTDTT VTYPEILRRY
60 70 80 90 100
PTDDHKDFIL PTDVTVFCQP EGCTTTSARL RKNARNDPQF FVFMLTEKDS
110 120 130 140 150
AKVRYGICLN FYQSFDRRST PKDEIKKVPD DAHHKKRDSH VSLTSLCFIS
160 170 180 190 200
HHPFVSIFHQ VLLLLKRIID SSNHRAAQRT GLKDVVWAIL TGHYNEPIVP
210 220 230 240 250
EVMKEIKEIE TWILMLLSSP VPVPGKTKVQ IEVMPMDLSQ VFEFALPDHT
260 270 280 290 300
RFTLIDFPLH IPFEILGIDM ALRVLTAAML EFKIVIQSRN YNAVSMCILS
310 320 330 340 350
IVALLYPLEY MFPVIPLLPA YMPSAEQLLL APTPFLIGVP SSFFHHRKIR
360 370 380 390 400
ELPSDVILVD LDTNCLQVPD DLYIPDLPEP DATHLKERLK NAINKMTTMT
410 420 430 440 450
VDNETSVTDA DFGIDIDSVD VACRVAMVQF FNSANVFGNF SEHTRTLRLY
460 470 480 490 500
PRPVVSLQTD SFLRSRPQCT QLITDLCRTQ AVEYFAECCL CPKNETFVRV
510 520 530 540 550
QAGIESAEQV GDKPKWFSES LMPVHFMVYP NNSTLDSAIR VYNAEIDNDD
560 570 580 590 600
YEDDSATSTE NSNSIDDLVF DENQVTDAGG EVTKPLAEVN YIYKEPMTLE
610 620 630 640 650
LPQSESVVSI DSSLSSGRSS PDSSLSTSAV DSEADFARLA DNLALKSNSQ
660 670 680 690 700
GAFSFDHGSD SEYESTPVSQ RRKTIHNPGS DASDTPTSRG SIKSGLRMKG
710 720 730 740 750
LTTLTDSGEK VLGPSLMNAI NGYAEKSQSV FSQVINKTAP KAQALKERTM
760 770 780 790 800
KPLANRIEQS QHIVRSKTQP NPTSQQTANQ QSKNQQTVKE FCDQALVGQS
810 820 830 840 850
VGMFSAPKLK RLMEDESLRE LVCSKLNLGL EVKLSEDEYV KEVQLTKGQF
860 870 880 890 900
KAYVKILKAC LEGIEVSFNT PGCCGFASVF HVLEIAHTHY WAMGGGEVIT
910 920 930 940 950
PSSSAPSTMT TPSEHSNDIL KESRPKLPAS TIDLRTPTKP LGQNVTPTST
960 970 980 990 1000
NNHEIAQSTR SPALPPPVPP REAPPIPKRN PPPLGAPPKV PEGARAPPPL
1010 1020 1030 1040 1050
PPRPKVKTTA VDETPQNLVP NNQPAQPSSP SFLADADEQT KPLLKPAPPT
1060 1070 1080 1090 1100
TLPVGKQEPC KVLPTPNEPV RHYIYQELIL AVQHQIWQNL QFWENAFVDL
1110 1120 1130 1140 1150
VAQEREIVGM DQEPSEMIDR YSALNDSEKK RLELEEDRLL STLLHNMTAY
1160 1170 1180 1190 1200
MIMCGTGQKA LQQKVRRLLG KAHIGLVCSK EINKLLDELP STQGNFIPLK
1210 1220 1230 1240 1250
PLGSRLVQKQ SFTVCPGQSS DGQMMFMEVC DDAVVLRSIT GAATERWWYE
1260 1270 1280 1290 1300
RLVNITYSPK TKILCLWRRH DDKVHMHKFH TKKCRELYQC MKAAMERAAA
1310 1320 1330 1340 1350
RGKVNVEGRA LGGEFPVHDT ETNQGGLLQV RCDGVAVIFA HNQIFIGLSN
1360 1370 1380 1390 1400
IKKCNTFGGN VFLLEEFDRK KGEIIQRRYF SQMADQICYA VLCVFSLAAA
GHKKEEHSK
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015555 | 1385 – 1409 | DQICY…EEHSK → VDIAWAMHRVFSVQFAISCQ KDTN in isoform b. CuratedAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93842 mRNA Translation: AAB52421.1 FO080298 Genomic DNA Translation: CCD62710.1 FO080298 Genomic DNA Translation: CCD62711.1 |
PIRi | T37188 |
RefSeqi | NP_001024342.1, NM_001029171.3 [O02626-2] NP_741710.1, NM_171618.3 [O02626-1] |
Genome annotation databases
EnsemblMetazoai | C02H7.3a.1; C02H7.3a.1; WBGene00000086 [O02626-1] C02H7.3b.1; C02H7.3b.1; WBGene00000086 [O02626-2] |
GeneIDi | 180420 |
KEGGi | cel:CELE_C02H7.3 |
UCSCi | C02H7.3a, c. elegans [O02626-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93842 mRNA Translation: AAB52421.1 FO080298 Genomic DNA Translation: CCD62710.1 FO080298 Genomic DNA Translation: CCD62711.1 |
PIRi | T37188 |
RefSeqi | NP_001024342.1, NM_001029171.3 [O02626-2] NP_741710.1, NM_171618.3 [O02626-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 45373, 1 interactor |
STRINGi | 6239.C02H7.3a |
Proteomic databases
EPDi | O02626 |
PaxDbi | O02626 |
PeptideAtlasi | O02626 |
PRIDEi | O02626 |
Genome annotation databases
EnsemblMetazoai | C02H7.3a.1; C02H7.3a.1; WBGene00000086 [O02626-1] C02H7.3b.1; C02H7.3b.1; WBGene00000086 [O02626-2] |
GeneIDi | 180420 |
KEGGi | cel:CELE_C02H7.3 |
UCSCi | C02H7.3a, c. elegans [O02626-1] |
Organism-specific databases
CTDi | 180420 |
WormBasei | C02H7.3a ; CE16806 ; WBGene00000086 ; aex-3 C02H7.3b ; CE30850 ; WBGene00000086 ; aex-3 |
Phylogenomic databases
eggNOGi | KOG3570, Eukaryota |
GeneTreei | ENSGT00940000156718 |
HOGENOMi | CLU_001270_0_0_1 |
InParanoidi | O02626 |
OMAi | ETFSMSK |
OrthoDBi | 162625at2759 |
PhylomeDBi | O02626 |
Enzyme and pathway databases
Reactomei | R-CEL-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
PROi | PR:O02626 |
Gene expression databases
Bgeei | WBGene00000086, Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues |
Family and domain databases
Gene3Di | 3.40.50.11500, 1 hit |
InterProi | View protein in InterPro IPR001194, cDENN_dom IPR005112, dDENN_dom IPR043153, DENN_C IPR039980, MADD IPR037516, Tripartite_DENN IPR005113, uDENN_dom |
PANTHERi | PTHR13008, PTHR13008, 1 hit |
Pfami | View protein in Pfam PF02141, DENN, 1 hit PF03456, uDENN, 1 hit |
SMARTi | View protein in SMART SM00801, dDENN, 1 hit SM00799, DENN, 1 hit SM00800, uDENN, 1 hit |
PROSITEi | View protein in PROSITE PS50211, DENN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MADD_CAEEL | |
Accessioni | O02626Primary (citable) accession number: O02626 Secondary accession number(s): Q27467, Q8MQF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 27, 2001 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families