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Protein

Putative 6-pyruvoyl tetrahydrobiopterin synthase

Gene

ptps-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin (By similarity).By similarity

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative 6-pyruvoyl tetrahydrobiopterin synthase (ptps-1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19ZincPROSITE-ProRule annotation1
Active sitei38Proton acceptorPROSITE-ProRule annotation1
Metal bindingi44ZincPROSITE-ProRule annotation1
Metal bindingi46ZincPROSITE-ProRule annotation1
Active sitei84Charge relay systemBy similarity1
Active sitei129Charge relay systemBy similarity1

GO - Molecular functioni

  • 6-pyruvoyltetrahydropterin synthase activity Source: WormBase
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: WormBase

Keywordsi

Molecular functionLyase
Biological processTetrahydrobiopterin biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
UniPathwayiUPA00849; UER00819

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:ptps-1
ORF Names:B0041.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0041.6a ; CE34237 ; WBGene00015010 ; ptps-1
B0041.6b ; CE39541 ; WBGene00015010 ; ptps-1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000579191 – 140Putative 6-pyruvoyl tetrahydrobiopterin synthaseAdd BLAST140

Proteomic databases

EPDiO02058
PaxDbiO02058
PeptideAtlasiO02058

Expressioni

Gene expression databases

BgeeiWBGene00015010

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-312091,EBI-312091

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi46692, 1 interactor
IntActiO02058, 1 interactor
STRINGi6239.B0041.6a

Structurei

Secondary structure

1140
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 19Combined sources14
Helixi28 – 35Combined sources8
Helixi36 – 39Combined sources4
Beta strandi44 – 57Combined sources14
Turni60 – 62Combined sources3
Helixi68 – 80Combined sources13
Turni81 – 84Combined sources4
Helixi87 – 90Combined sources4
Helixi92 – 95Combined sources4
Helixi101 – 115Combined sources15
Helixi119 – 121Combined sources3
Beta strandi122 – 130Combined sources9
Beta strandi133 – 137Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G64X-ray1.80A1-140[»]
ProteinModelPortaliO02058
SMRiO02058
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO02058

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105 Eukaryota
COG0720 LUCA
GeneTreeiENSGT00390000002752
HOGENOMiHOG000225069
InParanoidiO02058
KOiK01737
OMAiNIAVVIW
OrthoDBiEOG091G0TKH
PhylomeDBiO02058

Family and domain databases

Gene3Di3.30.479.10, 1 hit
InterProiView protein in InterPro
IPR007115 6-PTP_synth/QueD
IPR038418 6-PTP_synth/QueD_sf
IPR022470 PTPS_Cys_AS
PANTHERiPTHR12589 PTHR12589, 1 hit
PfamiView protein in Pfam
PF01242 PTPS, 1 hit
TIGRFAMsiTIGR00039 6PTHBS, 1 hit
PROSITEiView protein in PROSITE
PS00987 PTPS_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O02058-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW
60 70 80 90 100
KVKLRGEVDP TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST
110 120 130 140
SENVAIYMFE KLKSVMSNPS VLYKVTIEET PKNIFTYKGC
Note: No experimental confirmation available.
Length:140
Mass (Da):16,046
Last modified:October 10, 2003 - v2
Checksum:i7A677CE73A95882C
GO
Isoform b (identifier: O02058-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: No experimental confirmation available.
Show »
Length:137
Mass (Da):15,612
Checksum:iC5D18E885E24B16B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0192911 – 3Missing in isoform b. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080106 Genomic DNA Translation: CCD61251.1
FO080106 Genomic DNA Translation: CCD61252.1
PIRiT34033
RefSeqiNP_001040626.1, NM_001047161.1 [O02058-1]
NP_001040627.1, NM_001047162.2 [O02058-2]
UniGeneiCel.23560

Genome annotation databases

EnsemblMetazoaiB0041.6a; B0041.6a; WBGene00015010 [O02058-1]
GeneIDi181823
KEGGicel:CELE_B0041.6
UCSCiB0041.6b c. elegans [O02058-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTPS_CAEEL
AccessioniPrimary (citable) accession number: O02058
Secondary accession number(s): Q2MGF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2003
Last modified: March 28, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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