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Protein

Ras-related protein rab-11.1

Gene

rab-11.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21320697, PubMed:24843160). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21320697, PubMed:24843160). Involved in regulating the meiotic maturation of oocytes (PubMed:18472420). Plays a role in egg shell formation, regulating exocytosis of chrondroitin proteoglycans following fertilization (PubMed:18765566, PubMed:26506309). Controls cortical granule localization and targets them to the plasma membrane for exocytosis (PubMed:18765566). Acts as a major regulator of membrane delivery during cytokinesis (PubMed:18765566, PubMed:20116245, PubMed:26506309). Regulates the cytoskeleton by facilitating astral microtubule elongation and organization during metaphase to ensure proper spindle alignment and polarity in the first embryonic cell division (PubMed:18385514). Maintains normal endoplasmic reticulum morphology during metaphase (PubMed:18385514). Involved in vesicle formation and plasma membrane repair following exposure to pore forming toxins (PubMed:21320697). Regulates endocytic recycling (PubMed:24843160). May play a role in yolk receptor endocytosis in growing oocytes (PubMed:18354496, PubMed:26506309). Plays a role in the shedding of pathogen spores from intestinal cells via its involvement in spore fusion and endocytic trafficking (PubMed:24843160).8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26GTPBy similarity9
Nucleotide bindingi66 – 70GTPBy similarity5
Nucleotide bindingi124 – 127GTPBy similarity4
Nucleotide bindingi154 – 156GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: WormBase
  • GTP binding Source: WormBase

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-5620912 Anchoring of the basal body to the plasma membrane
R-CEL-5620916 VxPx cargo-targeting to cilium
R-CEL-8854214 TBC/RABGAPs
R-CEL-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein rab-11.1By similarity
Alternative name(s):
Rab GTPase rab-11.1Curated
Gene namesi
Name:rab-11.1Imported
ORF Names:F53G12.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF53G12.1 ; CE11006 ; WBGene00004274 ; rab-11.1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Zygotic lethal (PubMed:18765566). RNAi-mediated knockdown results in a reduced meiotic maturation rate of oocytes (PubMed:18472420). Embryos are multinucleated and osmotically sensitive with permeable and therefore defective eggshells (PubMed:18765566, PubMed:18385514). During the first cell cycle, 52% of embryos fail to extrude polar bodies, 83% of embryos display no or minimal pseudocleavage and the centrosomal-nuclear complex does not migrate to the center of embryos (PubMed:18385514). Animals display mitotic spindle alignment defects whereby in 73.9% of embryos, the P0 spindle does not rotate to the anterior-posterior axis of the embryo during anaphase, but during the metaphase to anaphase transition, the movements of the spindle are more abrupt with the spindle migrating to the posterior pole and then rebounding to the anterior-posterior axis (PubMed:18385514). In 17.4% of embryos the centrosomes are unstable and the spindle is displaced further towards the posterior pole (PubMed:18385514). Impaired localization of proteins important for establishing cell polarity such as par-2, par-3, gpr-1 and gpr-2 (PubMed:18385514). Altered microtubule organization and dynamics during metaphase including a greater distance between the growing microtubule plus ends and the posterior cortex, impeded microtubule growth with fewer growing astral microtubules reaching the cortex, and a reduced microtubule nucleation rate (PubMed:18385514). Slight defect in endoplasmic reticulum morphology whereby the large endoplasmic reticulum aggregates that normally form during metaphase and persist during anaphase, form, but do not persist during anaphase and disperse (PubMed:18385514). Mild endocytosis defect with an accumulation of the yolk protein vitellogenin in the pseudocoelom (PubMed:18354496). Impaired cortical granule (secretory vesicle) localization with granules abnormally clustered around the nuclear envelope of proximal oocytes, irregulary dispersed in the cytoplasm of oocytes and retained in the embryo following entry into the uterus (PubMed:18765566). Distrupted fusion of spores, containing intracellular pathogen N.parisii, with the apical cell membrane and thus disrupted clearance from intestinal cells (PubMed:24843160). RNAi-mediated knockdown in RNAi-sensitive mutants exposed to bacterial pore-forming toxin Cry5B results in intoxification and impaired plasma membrane repair (PubMed:21320697). RNAi-mediated knockdown in the intestine results in reduced survival upon exposure to Cry5B (PubMed:21320697).6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004356821 – 211Ras-related protein rab-11.1CuratedAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi208S-geranylgeranyl cysteineBy similarity1
Lipidationi209S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

EPDiO01803
PaxDbiO01803
PeptideAtlasiO01803
PRIDEiO01803

Expressioni

Tissue specificityi

Expressed weakly in sperm, but more predominantly in oocytes (PubMed:18472420). Expressed in the intestine (PubMed:24843160).2 Publications

Developmental stagei

Expressed during embryogenesis (PubMed:18385514, PubMed:20116245, PubMed:26506309). Transiently expressed during cytokinesis in embryos on the ingressing furrow and at the midbody during early abscission (PubMed:20116245). Expressed during the L4 stage and young adult stage of development (PubMed:22634595).4 Publications

Gene expression databases

BgeeiWBGene00004274 Expressed in 6 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Interactioni

Subunit structurei

Interacts with rei-1 and rei-2. The GDP-form preferentially binds to rei-1 and rei-2.1 Publication

Protein-protein interaction databases

DIPiDIP-24288N
IntActiO01803, 1 interactor
STRINGi6239.F53G12.1.2

Structurei

3D structure databases

ProteinModelPortaliO01803
SMRiO01803
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 48Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0087 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118841
HOGENOMiHOG000233968
InParanoidiO01803
KOiK07904
OMAiDISKHAT
OrthoDBiEOG091G0O1R
PhylomeDBiO01803

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

O01803-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSRDDEYDY LFKVVLIGDS GVGKSNLLSR FTRNEFNLES KSTIGVEFAT
60 70 80 90 100
RSISVEGKTV KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHVTYE
110 120 130 140 150
NVERWLKELR DHADQNIVIM LVGNKSDLRH LRAVPTDEAK IYAERNQLSF
160 170 180 190 200
IETSALDSTN VEAAFTNILT EIYKSVSNKH VGTDRQGYGG GSGTIIPSPA
210
SDPPKKQCCI P
Length:211
Mass (Da):23,429
Last modified:July 1, 1997 - v1
Checksum:iB0FD2837E42BE574
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ235189 mRNA Translation: AFP33153.1
BX284601 Genomic DNA Translation: CCD71701.1
PIRiT29035
RefSeqiNP_490675.1, NM_058274.3
UniGeneiCel.17482

Genome annotation databases

EnsemblMetazoaiF53G12.1.1; F53G12.1.1; WBGene00004274
F53G12.1.2; F53G12.1.2; WBGene00004274
GeneIDi171601
KEGGicel:CELE_F53G12.1
UCSCiF53G12.1 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ235189 mRNA Translation: AFP33153.1
BX284601 Genomic DNA Translation: CCD71701.1
PIRiT29035
RefSeqiNP_490675.1, NM_058274.3
UniGeneiCel.17482

3D structure databases

ProteinModelPortaliO01803
SMRiO01803
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24288N
IntActiO01803, 1 interactor
STRINGi6239.F53G12.1.2

Proteomic databases

EPDiO01803
PaxDbiO01803
PeptideAtlasiO01803
PRIDEiO01803

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF53G12.1.1; F53G12.1.1; WBGene00004274
F53G12.1.2; F53G12.1.2; WBGene00004274
GeneIDi171601
KEGGicel:CELE_F53G12.1
UCSCiF53G12.1 c. elegans

Organism-specific databases

CTDi171601
WormBaseiF53G12.1 ; CE11006 ; WBGene00004274 ; rab-11.1

Phylogenomic databases

eggNOGiKOG0087 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118841
HOGENOMiHOG000233968
InParanoidiO01803
KOiK07904
OMAiDISKHAT
OrthoDBiEOG091G0O1R
PhylomeDBiO01803

Enzyme and pathway databases

ReactomeiR-CEL-5620912 Anchoring of the basal body to the plasma membrane
R-CEL-5620916 VxPx cargo-targeting to cilium
R-CEL-8854214 TBC/RABGAPs
R-CEL-8873719 RAB geranylgeranylation

Miscellaneous databases

PROiPR:O01803

Gene expression databases

BgeeiWBGene00004274 Expressed in 6 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRB11A_CAEEL
AccessioniPrimary (citable) accession number: O01803
Secondary accession number(s): I7EVK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 16, 2016
Last sequence update: July 1, 1997
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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