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Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

UCH

Organism
Aplysia californica (California sea hare)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophilePROSITE-ProRule annotation1
Active sitei164Proton donorPROSITE-ProRule annotation1
Sitei179Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC12.003

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:UCH
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110681 – 214Ubiquitin carboxyl-terminal hydrolaseAdd BLAST214

Structurei

3D structure databases

ProteinModelPortaliO01391
SMRiO01391
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
PANTHERiPTHR10589 PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PRINTSiPR00707 UBCTHYDRLASE
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00140 UCH_1, 1 hit

Sequencei

Sequence statusi: Complete.

O01391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA
60 70 80 90 100
AALVLLFPDD KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA
110 120 130 140 150
NNENVIPFDA AKHFKTFLEK TKPLNPEERA KHLEQDNLMG AAHGDCAQEG
160 170 180 190 200
DTQAPSQDEH VKSHFVALVH CNGTLYELDG RKEAPVVHGT TSADTFLEDA
210
AEVVKKFMAR DPEI
Length:214
Mass (Da):23,703
Last modified:July 1, 1997 - v1
Checksum:i07ACDEDAADB05D6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90177 mRNA Translation: AAB52410.1
RefSeqiNP_001191493.1, NM_001204564.1
UniGeneiAcl.28

Genome annotation databases

GeneIDi100533253

Similar proteinsi

Entry informationi

Entry nameiUCHL_APLCA
AccessioniPrimary (citable) accession number: O01391
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: June 20, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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