UniProtKB - O01382 (DRICE_DROME)
Protein
Caspase
Gene
Drice
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Involved in the activation cascade of caspases responsible for apoptosis execution. Acts downstream of rpr. Cleaves baculovirus p35 and lamin DmO in vitro.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 169 | By similarity | 1 | |
Active sitei | 211 | By similarity | 1 |
GO - Molecular functioni
- BIR domain binding Source: FlyBase
- cysteine-type endopeptidase activity Source: FlyBase
- cysteine-type endopeptidase activity involved in apoptotic process Source: FlyBase
- cysteine-type endopeptidase activity involved in execution phase of apoptosis Source: FlyBase
GO - Biological processi
- apoptotic process Source: GO_Central
- negative regulation of apoptotic process Source: FlyBase
- neuron remodeling Source: FlyBase
- nurse cell apoptotic process Source: FlyBase
- positive regulation of compound eye retinal cell programmed cell death Source: FlyBase
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: FlyBase
- programmed cell death Source: FlyBase
- programmed cell death involved in cell development Source: FlyBase
- proteolysis Source: FlyBase
- response to X-ray Source: FlyBase
- salivary gland histolysis Source: FlyBase
- spermatid differentiation Source: FlyBase
- sterol regulatory element binding protein cleavage Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-DME-111459, Activation of caspases through apoptosome-mediated cleavage R-DME-111465, Apoptotic cleavage of cellular proteins R-DME-111469, SMAC, XIAP-regulated apoptotic response R-DME-352238, Breakdown of the nuclear lamina |
Protein family/group databases
MEROPSi | C14.015 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Drice Synonyms:ICE ORF Names:CG7788 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0019972, Drice |
Subcellular locationi
Nucleus
- nucleus Source: FlyBase
Plasma Membrane
- plasma membrane Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | A → V: Abolishes binding to Diap2 but has no effect on Drice processing or activity. 1 Publication | 1 | |
Mutagenesisi | 211 | C → A: No effect on binding to Diap2 but may effect stability of complex formed with Diap2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000004666 | 1 – 28 | By similarityAdd BLAST | 28 | |
ChainiPRO_0000004667 | 29 – 217 | Caspase subunit p21By similarityAdd BLAST | 189 | |
PropeptideiPRO_0000004668 | 218 – 230 | By similarityAdd BLAST | 13 | |
ChainiPRO_0000004669 | 231 – 339 | Caspase subunit p12By similarityAdd BLAST | 109 |
Keywords - PTMi
ZymogenProteomic databases
PaxDbi | O01382 |
PRIDEi | O01382 |
Expressioni
Developmental stagei
Expressed at all stages where apoptosis occurs.
Gene expression databases
Bgeei | FBgn0019972, Expressed in embryo and 47 other tissues |
Genevisiblei | O01382, DM |
Interactioni
Subunit structurei
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 21 kDa (p21) and a 12 kDa (p12) subunit. Inactive pro-form can homodimerize. Dronc and Drice can form a stable complex (PubMed:10675329).
Interacts with Diap2 (via BIR3 domain) to form a stable complex (PubMed:18166655).
2 PublicationsBinary interactionsi
O01382
With | #Exp. | IntAct |
---|---|---|
Dronc [Q9XYF4] | 3 | EBI-91422,EBI-108311 |
GO - Molecular functioni
- BIR domain binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 68378, 24 interactors |
DIPi | DIP-21838N |
ELMi | O01382 |
IntActi | O01382, 2 interactors |
MINTi | O01382 |
STRINGi | 7227.FBpp0084848 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O01382 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C14A family.Curated
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | O01382 |
OMAi | SWRNTTR |
OrthoDBi | 984395at2759 |
PhylomeDBi | O01382 |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O01382-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDATNNGESA DQVGIRVGNP EQPNDHTDAL GSVGSGGAGS SGLVAGSSHP
60 70 80 90 100
YGSGAIGQLA NGYSSPSSSY RKNVAKMVTD RHAAEYNMRH KNRGMALIFN
110 120 130 140 150
HEHFEVPTLK SRAGTNVDCE NLTRVLKQLD FEVTVYKDCR YKDILRTIEY
160 170 180 190 200
AASQNHSDSD CILVAILSHG EMGYIYAKDT QYKLDNIWSF FTANHCPSLA
210 220 230 240 250
GKPKLFFIQA CQGDRLDGGV TMQRSQTETD GDSSMSYKIP VHADFLIAYS
260 270 280 290 300
TVPGFYSWRN TTRGSWFMQS LCAELAANGK RLDILTLLTF VCQRVAVDFE
310 320 330
SCTPDTPEMH QQKQIPCITT MLTRILRFSD KQLAPAGRV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 151 | A → S in CAA72937 (PubMed:9184225).Curated | 1 | |
Sequence conflicti | 265 | S → T in CAA72937 (PubMed:9184225).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12261 mRNA Translation: CAA72937.1 AE014297 Genomic DNA Translation: AAF56939.1 AY058451 mRNA Translation: AAL13680.1 |
RefSeqi | NP_524551.2, NM_079827.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0085482; FBpp0084848; FBgn0019972 |
GeneIDi | 43514 |
KEGGi | dme:Dmel_CG7788 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12261 mRNA Translation: CAA72937.1 AE014297 Genomic DNA Translation: AAF56939.1 AY058451 mRNA Translation: AAL13680.1 |
RefSeqi | NP_524551.2, NM_079827.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SIP | X-ray | 3.50 | A/C | 78-230 | [»] | |
B/D | 231-339 | [»] | ||||
3SIR | X-ray | 2.68 | A/B/C/D | 78-332 | [»] | |
SMRi | O01382 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 68378, 24 interactors |
DIPi | DIP-21838N |
ELMi | O01382 |
IntActi | O01382, 2 interactors |
MINTi | O01382 |
STRINGi | 7227.FBpp0084848 |
Protein family/group databases
MEROPSi | C14.015 |
Proteomic databases
PaxDbi | O01382 |
PRIDEi | O01382 |
Genome annotation databases
EnsemblMetazoai | FBtr0085482; FBpp0084848; FBgn0019972 |
GeneIDi | 43514 |
KEGGi | dme:Dmel_CG7788 |
Organism-specific databases
CTDi | 43514 |
FlyBasei | FBgn0019972, Drice |
Phylogenomic databases
eggNOGi | KOG3573, Eukaryota |
GeneTreei | ENSGT00940000153232 |
HOGENOMi | CLU_036904_2_0_1 |
InParanoidi | O01382 |
OMAi | SWRNTTR |
OrthoDBi | 984395at2759 |
PhylomeDBi | O01382 |
Enzyme and pathway databases
Reactomei | R-DME-111459, Activation of caspases through apoptosome-mediated cleavage R-DME-111465, Apoptotic cleavage of cellular proteins R-DME-111469, SMAC, XIAP-regulated apoptotic response R-DME-352238, Breakdown of the nuclear lamina |
Miscellaneous databases
BioGRID-ORCSi | 43514, 0 hits in 3 CRISPR screens |
GenomeRNAii | 43514 |
PROi | PR:O01382 |
Gene expression databases
Bgeei | FBgn0019972, Expressed in embryo and 47 other tissues |
Genevisiblei | O01382, DM |
Family and domain databases
CDDi | cd00032, CASc, 1 hit |
InterProi | View protein in InterPro IPR029030, Caspase-like_dom_sf IPR033139, Caspase_cys_AS IPR016129, Caspase_his_AS IPR002398, Pept_C14 IPR002138, Pept_C14_p10 IPR001309, Pept_C14_p20 IPR015917, Pept_C14A |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
PRINTSi | PR00376, IL1BCENZYME |
SMARTi | View protein in SMART SM00115, CASc, 1 hit |
SUPFAMi | SSF52129, SSF52129, 1 hit |
PROSITEi | View protein in PROSITE PS01122, CASPASE_CYS, 1 hit PS01121, CASPASE_HIS, 1 hit PS50207, CASPASE_P10, 1 hit PS50208, CASPASE_P20, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DRICE_DROME | |
Accessioni | O01382Primary (citable) accession number: O01382 Secondary accession number(s): Q9VAH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families