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UniProtKB - O00910 (STATA_DICDI)
Protein
Signal transducer and activator of transcription A
Gene
dstA
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
Transcription factor that binds to 5'-TTGAATTGA-3' elements in the promoter region of target genes. Functions as repressor of the ecmB gene. Regulates the differentiation of prestalk cells during development.
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 443 – 487 | Add BLAST | 45 |
GO - Molecular functioni
- DNA binding Source: dictyBase
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- identical protein binding Source: dictyBase
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- culmination involved in sorocarp development Source: dictyBase
- defense response Source: GO_Central
- negative regulation of transcription, DNA-templated Source: dictyBase
- positive regulation of gene expression Source: dictyBase
- receptor signaling pathway via JAK-STAT Source: GO_Central
- receptor signaling pathway via STAT Source: dictyBase
- regulation of cell population proliferation Source: GO_Central
- regulation of gene expression Source: dictyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- sorocarp development Source: dictyBase
- sorocarp stalk cell differentiation Source: dictyBase
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DDI-1059683, Interleukin-6 signaling R-DDI-1169408, ISG15 antiviral mechanism R-DDI-201556, Signaling by ALK R-DDI-3249367, STAT6-mediated induction of chemokines R-DDI-6783783, Interleukin-10 signaling R-DDI-6785807, Interleukin-4 and Interleukin-13 signaling R-DDI-877300, Interferon gamma signaling R-DDI-877312, Regulation of IFNG signaling R-DDI-8854691, Interleukin-20 family signaling R-DDI-8983432, Interleukin-15 signaling R-DDI-8984722, Interleukin-35 Signalling R-DDI-8985947, Interleukin-9 signaling R-DDI-9008059, Interleukin-37 signaling R-DDI-9020591, Interleukin-12 signaling R-DDI-9020933, Interleukin-23 signaling R-DDI-9020956, Interleukin-27 signaling R-DDI-909733, Interferon alpha/beta signaling R-DDI-9701898, STAT3 nuclear events downstream of ALK signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Signal transducer and activator of transcription AAlternative name(s): Dd-STATa STAT5 homolog A |
Gene namesi | Name:dstA Synonyms:stat5, statA ORF Names:DDB_G0281381 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0281381, dstA |
Subcellular locationi
Nucleus
Cytoplasm and Cytosol
Note: Cytoplasmic in growing cells. Translocated into the nucleus in response to cAMP-induced tyrosine phosphorylation. Nuclear at the tight mound stage and in the upper, prestalk region of tipped aggregates and in cells at the tip of the slug. Subject to crm1-dependent nuclear export.
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Cells are hypersensitive to the chlorinated hexaphenone DIF. They form slugs, but there is little or no stalk cell differentiation. After several days of developmental arrest very small spore masses appear that are supported by columns of apparently undifferentiated cells.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 443 | K → D: Loss of DNA binding. 1 Publication | 1 | |
Mutagenesisi | 449 – 450 | RK → DD: About 3-fold reduced DNA binding. 1 Publication | 2 | |
Mutagenesisi | 484 | N → A: Loss of DNA binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000328082 | 1 – 707 | Signal transducer and activator of transcription AAdd BLAST | 707 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 702 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Tyrosine phosphorylated in response to cAMP. Not tyrosine phosphorylated in growing cells. Tyrosine phosphorylation is first detected at the tight mound stage, continues throughout the slug stage and early culmination, and starts to decrease at mid-culmination. Barely detectable in fruiting bodies.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O00910 |
PTM databases
iPTMneti | O00910 |
Expressioni
Developmental stagei
Constitutively expressed with a slight increase during the tight mound stage (at protein level). Detected at very low levels in growing cells and aggregates up to the loose mound stage. Highly expressed in tipped aggregates and in the Mexican hat stage. Expressed at lower levels in early and late culminants and in fruiting bodies.2 Publications
Interactioni
Subunit structurei
Monomer, in the absence of tyrosine phosphorylation. Homodimer, or heterodimer with another family member, when tyrosine phosphorylated.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 380 | Interaction with DNABy similarity | 1 | |
Sitei | 577 | Interaction with DNABy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: dictyBase
Protein-protein interaction databases
STRINGi | 44689.DDB0215388 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O00910 |
SMRi | O00910 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O00910 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 583 – 686 | SH2PROSITE-ProRule annotationAdd BLAST | 104 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 70 – 246 | DisorderedSequence analysisAdd BLAST | 177 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 242 – 356 | 1 PublicationAdd BLAST | 115 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 70 – 128 | Polar residuesSequence analysisAdd BLAST | 59 | |
Compositional biasi | 136 – 246 | Polar residuesSequence analysisAdd BLAST | 111 |
Sequence similaritiesi
Belongs to the transcription factor STAT family.Curated
Keywords - Domaini
Coiled coil, SH2 domainPhylogenomic databases
eggNOGi | KOG3667, Eukaryota |
HOGENOMi | CLU_390520_0_0_1 |
InParanoidi | O00910 |
OMAi | QMNTEMS |
PhylomeDBi | O00910 |
Family and domain databases
Gene3Di | 2.60.40.340, 1 hit 3.30.505.10, 1 hit |
IDEALi | IID50264 |
InterProi | View protein in InterPro IPR041604, EF-hand_12 IPR008967, p53-like_TF_DNA-bd IPR037059, RHD_DNA_bind_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR001217, STAT IPR015988, STAT_TF_coiled-coil IPR015347, STAT_TF_homologue_coiled-coil IPR041410, STATa_Ig |
PANTHERi | PTHR11801, PTHR11801, 3 hits |
Pfami | View protein in Pfam PF09267, Dict-STAT-coil, 1 hit PF17901, EF-hand_12, 1 hit PF00017, SH2, 1 hit PF18214, STATa_Ig, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF47655, SSF47655, 1 hit SSF49417, SSF49417, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
i Sequence
Sequence statusi: Complete.
O00910-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAEFSMDD FEDTFDSNAT ISTKDLFEGS DRLPLNQSIN TTIQNLYLPN
60 70 80 90 100
GGFAIGDQSQ QQYYQAMPPL NQSDQFNLGR SNNLTPRTNQ LQQLQQQQQQ
110 120 130 140 150
QQQPQQQQQQ QTYGTQSPIH MSQTPSSPLS SPLPSPTPFS RQQSYNNNNS
160 170 180 190 200
NNTSSSQNYN NNNININNNN NNNNTNNNNN NNNGNNSNGN NGNNNNNNNN
210 220 230 240 250
NNNNNTNNNN NNNQQQQQQQ QQQQQQQQQQ QQQQQQGNPN LSSPQPILDT
260 270 280 290 300
IYKLLSEQEQ TLVQMIHEQS LLLNRLPPTL DENSLAPLKS LSQKQITLSG
310 320 330 340 350
QMNTEMSALD ATKKGMILEP TDLAKLFALK QDLQIQFKQL SLLHNEIQSI
360 370 380 390 400
LNPQHSAPKP NVALVLKSQP FPVVISKGKQ LGENQLVVLV LTGARSNFHI
410 420 430 440 450
NGPVKATMIC DSHPTNKNNP TTPLEMDSQP IYPATLTAHF PLKFLAGTRK
460 470 480 490 500
CSVNLKFGVN IRDLDNVTTT VESDASNPFV VITNECQWEG SAGVLLKKDA
510 520 530 540 550
FDGQLEITWA QFINTLQRHF LIATKQDPVR PKRPLSSYDL KYIQTHFFGN
560 570 580 590 600
RSIIHQQDFD KFWVWFGKSM QTLRYQRHIS TLWQEGIIYG YMGRQEVNDA
610 620 630 640 650
LQNQDPGTFI IRFSERNPGQ FGIAYIGVEM PARIKHYLVQ PNDTAAAKKT
660 670 680 690 700
FPDFLSEHSQ FVNLLQWTKD TNGAPRFLKL HKDTALGSFA PKRTAPVPVG
GYEPLNS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13097 mRNA Translation: CAA73551.1 AAFI02000041 Genomic DNA Translation: EAL66672.1 |
RefSeqi | XP_640661.1, XM_635569.1 |
Genome annotation databases
EnsemblProtistsi | EAL66672; EAL66672; DDB_G0281381 |
GeneIDi | 8623045 |
KEGGi | ddi:DDB_G0281381 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y13097 mRNA Translation: CAA73551.1 AAFI02000041 Genomic DNA Translation: EAL66672.1 |
RefSeqi | XP_640661.1, XM_635569.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UUR | X-ray | 2.70 | A | 235-707 | [»] | |
1UUS | X-ray | 2.80 | A | 235-707 | [»] | |
AlphaFoldDBi | O00910 | |||||
SMRi | O00910 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0215388 |
PTM databases
iPTMneti | O00910 |
Proteomic databases
PaxDbi | O00910 |
Genome annotation databases
EnsemblProtistsi | EAL66672; EAL66672; DDB_G0281381 |
GeneIDi | 8623045 |
KEGGi | ddi:DDB_G0281381 |
Organism-specific databases
dictyBasei | DDB_G0281381, dstA |
Phylogenomic databases
eggNOGi | KOG3667, Eukaryota |
HOGENOMi | CLU_390520_0_0_1 |
InParanoidi | O00910 |
OMAi | QMNTEMS |
PhylomeDBi | O00910 |
Enzyme and pathway databases
Reactomei | R-DDI-1059683, Interleukin-6 signaling R-DDI-1169408, ISG15 antiviral mechanism R-DDI-201556, Signaling by ALK R-DDI-3249367, STAT6-mediated induction of chemokines R-DDI-6783783, Interleukin-10 signaling R-DDI-6785807, Interleukin-4 and Interleukin-13 signaling R-DDI-877300, Interferon gamma signaling R-DDI-877312, Regulation of IFNG signaling R-DDI-8854691, Interleukin-20 family signaling R-DDI-8983432, Interleukin-15 signaling R-DDI-8984722, Interleukin-35 Signalling R-DDI-8985947, Interleukin-9 signaling R-DDI-9008059, Interleukin-37 signaling R-DDI-9020591, Interleukin-12 signaling R-DDI-9020933, Interleukin-23 signaling R-DDI-9020956, Interleukin-27 signaling R-DDI-909733, Interferon alpha/beta signaling R-DDI-9701898, STAT3 nuclear events downstream of ALK signaling |
Miscellaneous databases
EvolutionaryTracei | O00910 |
PROi | PR:O00910 |
Family and domain databases
Gene3Di | 2.60.40.340, 1 hit 3.30.505.10, 1 hit |
IDEALi | IID50264 |
InterProi | View protein in InterPro IPR041604, EF-hand_12 IPR008967, p53-like_TF_DNA-bd IPR037059, RHD_DNA_bind_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR001217, STAT IPR015988, STAT_TF_coiled-coil IPR015347, STAT_TF_homologue_coiled-coil IPR041410, STATa_Ig |
PANTHERi | PTHR11801, PTHR11801, 3 hits |
Pfami | View protein in Pfam PF09267, Dict-STAT-coil, 1 hit PF17901, EF-hand_12, 1 hit PF00017, SH2, 1 hit PF18214, STATa_Ig, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit |
SUPFAMi | SSF47655, SSF47655, 1 hit SSF49417, SSF49417, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50001, SH2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STATA_DICDI | |
Accessioni | O00910Primary (citable) accession number: O00910 Secondary accession number(s): Q54U00 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | July 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families