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Protein

Ubiquitin-conjugating enzyme E2 C

Gene

UBE2C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.6 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation2 Publications
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin-like protein ligase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiO00762
SIGNORiO00762
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 C (EC:2.3.2.232 Publications)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.241 Publication)
E2 ubiquitin-conjugating enzyme C
UbcH10
Ubiquitin carrier protein C
Ubiquitin-protein ligase C
Gene namesi
Name:UBE2C
Synonyms:UBCH10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000175063.16
HGNCiHGNC:15937 UBE2C
MIMi605574 gene
neXtProtiNX_O00762

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114C → S: Loss of function; inhibition of cyclin-B degradation. 2 Publications1

Organism-specific databases

DisGeNETi11065
OpenTargetsiENSG00000175063
PharmGKBiPA38057

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000825602 – 179Ubiquitin-conjugating enzyme E2 CAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3PhosphoserineCombined sources1

Post-translational modificationi

Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO00762
PaxDbiO00762
PeptideAtlasiO00762
PRIDEiO00762
ProteomicsDBi12714
48021
TopDownProteomicsiO00762-1 [O00762-1]
O00762-3 [O00762-3]

PTM databases

iPTMnetiO00762
PhosphoSitePlusiO00762

Expressioni

Gene expression databases

BgeeiENSG00000175063 Expressed in 179 organ(s), highest expression level in oocyte
CleanExiHS_UBE2C
ExpressionAtlasiO00762 baseline and differential
GenevisibleiO00762 HS

Organism-specific databases

HPAiCAB011464
CAB035990
HPA034569
HPA054975

Interactioni

Subunit structurei

Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116249, 69 interactors
DIPiDIP-52725N
IntActiO00762, 10 interactors
STRINGi9606.ENSP00000348838

Structurei

Secondary structure

1179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO00762
SMRiO00762
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00762

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
GeneTreeiENSGT00930000150941
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiO00762
KOiK06688
OMAiELMQLMM
OrthoDBiEOG091G0M7S
PhylomeDBiO00762
TreeFamiTF101116

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O00762-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASQNRDPAA TSVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI
60 70 80 90 100
SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT
110 120 130 140 150
PCYHPNVDTQ GNICLDILKE KWSALYDVRT ILLSIQSLLG EPNIDSPLNT
160 170
HAAELWKNPT AFKKYLQETY SKQVTSQEP
Length:179
Mass (Da):19,652
Last modified:July 1, 1997 - v1
Checksum:i0B6F58A1F0665D9A
GO
Isoform 2 (identifier: O00762-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):15,765
Checksum:i194F276AD75D5168
GO
Isoform 3 (identifier: O00762-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-72: Missing.

Note: No experimental confirmation available.
Show »
Length:150
Mass (Da):16,676
Checksum:i5410B7BA11DFC35E
GO
Isoform 4 (identifier: O00762-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     73-140: VYEDLRYKLS...ILLSIQSLLG → AVGSIRTSST...LTLQLFLQMP

Note: No experimental confirmation available.
Show »
Length:161
Mass (Da):17,373
Checksum:iD8F52001401888DA
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WVK1A0A087WVK1_HUMAN
Ubiquitin-conjugating enzyme E2 C
UBE2C
132Annotation score:
A0A0A0MSE8A0A0A0MSE8_HUMAN
Ubiquitin-conjugating enzyme E2 C
UBE2C
79Annotation score:
K4DI81K4DI81_HUMAN
Ubiquitin-conjugating enzyme E2 C
UBE2C hCG_38372
50Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00769425G → D. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0456471 – 39Missing in isoform 2. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_04564844 – 72Missing in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_04564973 – 140VYEDL…QSLLG → AVGSIRTSSTVCLLSGPRET QDSSKPLVWGLGWDMRLLLE LTLQLFLQMP in isoform 4. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73379 mRNA Translation: AAB53362.1
BT007300 mRNA Translation: AAP35964.1
AL050348 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75804.1
CH471077 Genomic DNA Translation: EAW75805.1
CH471077 Genomic DNA Translation: EAW75806.1
CH471077 Genomic DNA Translation: EAW75807.1
BC007656 mRNA Translation: AAH07656.1
BC016292 mRNA Translation: AAH16292.1
BC050736 mRNA Translation: AAH50736.1
BI858659 mRNA No translation available.
BM556795 mRNA No translation available.
BU844974 mRNA No translation available.
CCDSiCCDS13370.1 [O00762-1]
CCDS13371.1 [O00762-4]
CCDS13372.1 [O00762-3]
CCDS13374.1 [O00762-2]
RefSeqiNP_001268670.1, NM_001281741.1
NP_001268671.1, NM_001281742.1
NP_008950.1, NM_007019.3 [O00762-1]
NP_861515.1, NM_181799.2 [O00762-4]
NP_861516.1, NM_181800.2 [O00762-3]
NP_861517.1, NM_181801.3 [O00762-2]
UniGeneiHs.93002

Genome annotation databases

EnsembliENST00000335046; ENSP00000335674; ENSG00000175063 [O00762-4]
ENST00000352551; ENSP00000333975; ENSG00000175063 [O00762-3]
ENST00000356455; ENSP00000348838; ENSG00000175063 [O00762-1]
ENST00000372568; ENSP00000361649; ENSG00000175063 [O00762-2]
GeneIDi11065
KEGGihsa:11065
UCSCiuc002xpl.5 human [O00762-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73379 mRNA Translation: AAB53362.1
BT007300 mRNA Translation: AAP35964.1
AL050348 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75804.1
CH471077 Genomic DNA Translation: EAW75805.1
CH471077 Genomic DNA Translation: EAW75806.1
CH471077 Genomic DNA Translation: EAW75807.1
BC007656 mRNA Translation: AAH07656.1
BC016292 mRNA Translation: AAH16292.1
BC050736 mRNA Translation: AAH50736.1
BI858659 mRNA No translation available.
BM556795 mRNA No translation available.
BU844974 mRNA No translation available.
CCDSiCCDS13370.1 [O00762-1]
CCDS13371.1 [O00762-4]
CCDS13372.1 [O00762-3]
CCDS13374.1 [O00762-2]
RefSeqiNP_001268670.1, NM_001281741.1
NP_001268671.1, NM_001281742.1
NP_008950.1, NM_007019.3 [O00762-1]
NP_861515.1, NM_181799.2 [O00762-4]
NP_861516.1, NM_181800.2 [O00762-3]
NP_861517.1, NM_181801.3 [O00762-2]
UniGeneiHs.93002

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7KX-ray1.95A/B1-179[»]
4YIIX-ray1.80U27-179[»]
5A31electron microscopy4.30Q29-173[»]
5KHRelectron microscopy6.10Q1-179[»]
5L9Uelectron microscopy6.40U1-179[»]
ProteinModelPortaliO00762
SMRiO00762
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116249, 69 interactors
DIPiDIP-52725N
IntActiO00762, 10 interactors
STRINGi9606.ENSP00000348838

PTM databases

iPTMnetiO00762
PhosphoSitePlusiO00762

Proteomic databases

EPDiO00762
PaxDbiO00762
PeptideAtlasiO00762
PRIDEiO00762
ProteomicsDBi12714
48021
TopDownProteomicsiO00762-1 [O00762-1]
O00762-3 [O00762-3]

Protocols and materials databases

DNASUi11065
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335046; ENSP00000335674; ENSG00000175063 [O00762-4]
ENST00000352551; ENSP00000333975; ENSG00000175063 [O00762-3]
ENST00000356455; ENSP00000348838; ENSG00000175063 [O00762-1]
ENST00000372568; ENSP00000361649; ENSG00000175063 [O00762-2]
GeneIDi11065
KEGGihsa:11065
UCSCiuc002xpl.5 human [O00762-1]

Organism-specific databases

CTDi11065
DisGeNETi11065
EuPathDBiHostDB:ENSG00000175063.16
GeneCardsiUBE2C
HGNCiHGNC:15937 UBE2C
HPAiCAB011464
CAB035990
HPA034569
HPA054975
MIMi605574 gene
neXtProtiNX_O00762
OpenTargetsiENSG00000175063
PharmGKBiPA38057
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
GeneTreeiENSGT00930000150941
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiO00762
KOiK06688
OMAiELMQLMM
OrthoDBiEOG091G0M7S
PhylomeDBiO00762
TreeFamiTF101116

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiO00762
SIGNORiO00762

Miscellaneous databases

ChiTaRSiUBE2C human
EvolutionaryTraceiO00762
GeneWikiiUBE2C
GenomeRNAii11065
PROiPR:O00762
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175063 Expressed in 179 organ(s), highest expression level in oocyte
CleanExiHS_UBE2C
ExpressionAtlasiO00762 baseline and differential
GenevisibleiO00762 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2C_HUMAN
AccessioniPrimary (citable) accession number: O00762
Secondary accession number(s): A6NP33, E1P5N7, G3XAB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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