UniProtKB - O00748 (EST2_HUMAN)
Cocaine esterase
CES2
Functioni
Catalytic activityi
Kineticsi
- KM=0.39 mM for cocaine1 Publication
- KM=0.15 mM for 4-methylumbelliferyl acetate1 Publication
- KM=6.8 mM for heroin1 Publication
- KM=0.13 mM for 6-monoacetylmorphine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 228 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 345 | Charge relay systemBy similarity | 1 | |
Active sitei | 457 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- carboxylic ester hydrolase activity Source: BHF-UCL
- methyl indole-3-acetate esterase activity Source: UniProtKB-EC
- methylumbelliferyl-acetate deacetylase activity Source: UniProtKB-EC
GO - Biological processi
- catabolic process Source: ProtInc
- prostaglandin metabolic process Source: BHF-UCL
- xenobiotic metabolic process Source: Reactome
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Enzyme and pathway databases
BRENDAi | 3.1.1.1 2681 3.1.1.84 2681 |
Reactomei | R-HSA-211945 Phase I - Functionalization of compounds |
SABIO-RKi | O00748 |
Protein family/group databases
ESTHERi | human-CES2 Carb_B_Chordata |
MEROPSi | S09.984 |
Chemistry databases
SwissLipidsi | SLP:000001424 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CES2 Synonyms:ICE |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000172831.11 |
HGNCi | HGNC:1864 CES2 |
MIMi | 605278 gene |
neXtProti | NX_O00748 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: ProtInc
- endoplasmic reticulum lumen Source: Reactome
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Organism-specific databases
DisGeNETi | 8824 |
OpenTargetsi | ENSG00000172831 |
PharmGKBi | PA377 |
Chemistry databases
ChEMBLi | CHEMBL3180 |
DrugBanki | DB06695 Dabigatran etexilate DB00762 Irinotecan DB00688 Mycophenolate mofetil DB06209 Prasugrel |
Polymorphism and mutation databases
BioMutai | CES2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000008572 | 27 – 559 | Cocaine esteraseAdd BLAST | 533 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 27 | Pyrrolidone carboxylic acidBy similarity | 1 | |
Disulfide bondi | 95 ↔ 123 | By similarity | ||
Glycosylationi | 111 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 276 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 280 ↔ 291 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acidProteomic databases
EPDi | O00748 |
jPOSTi | O00748 |
MaxQBi | O00748 |
PaxDbi | O00748 |
PeptideAtlasi | O00748 |
PRIDEi | O00748 |
ProteomicsDBi | 48014 48015 [O00748-2] |
PTM databases
iPTMneti | O00748 |
PhosphoSitePlusi | O00748 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000172831 Expressed in 236 organ(s), highest expression level in jejunal mucosa |
ExpressionAtlasi | O00748 baseline and differential |
Genevisiblei | O00748 HS |
Organism-specific databases
HPAi | HPA018897 |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGridi | 114351, 1 interactor |
IntActi | O00748, 2 interactors |
STRINGi | 9606.ENSP00000317842 |
Chemistry databases
BindingDBi | O00748 |
Structurei
3D structure databases
ProteinModelPortali | O00748 |
SMRi | O00748 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 556 – 559 | Prevents secretion from ERSequence analysis | 4 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1516 Eukaryota COG2272 LUCA |
GeneTreei | ENSGT00940000153793 |
HOGENOMi | HOG000091866 |
HOVERGENi | HBG008839 |
InParanoidi | O00748 |
KOi | K03927 |
OMAi | GASAYWP |
OrthoDBi | 754103at2759 |
PhylomeDBi | O00748 |
TreeFami | TF315470 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058 AB_hydrolase IPR002018 CarbesteraseB IPR019826 Carboxylesterase_B_AS IPR019819 Carboxylesterase_B_CS |
Pfami | View protein in Pfam PF00135 COesterase, 1 hit |
SUPFAMi | SSF53474 SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122 CARBOXYLESTERASE_B_1, 1 hit PS00941 CARBOXYLESTERASE_B_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing and alternative initiation. AlignAdd to basketThis entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MRLHRLRARL SAVACGLLLL LVRGQGQDSA SPIRTTHTGQ VLGSLVHVKG
60 70 80 90 100
ANAGVQTFLG IPFAKPPLGP LRFAPPEPPE SWSGVRDGTT HPAMCLQDLT
110 120 130 140 150
AVESEFLSQF NMTFPSDSMS EDCLYLSIYT PAHSHEGSNL PVMVWIHGGA
160 170 180 190 200
LVFGMASLYD GSMLAALENV VVVIIQYRLG VLGFFSTGDK HATGNWGYLD
210 220 230 240 250
QVAALRWVQQ NIAHFGGNPD RVTIFGESAG GTSVSSLVVS PISQGLFHGA
260 270 280 290 300
IMESGVALLP GLIASSADVI STVVANLSAC DQVDSEALVG CLRGKSKEEI
310 320 330 340 350
LAINKPFKMI PGVVDGVFLP RHPQELLASA DFQPVPSIVG VNNNEFGWLI
360 370 380 390 400
PKVMRIYDTQ KEMDREASQA ALQKMLTLLM LPPTFGDLLR EEYIGDNGDP
410 420 430 440 450
QTLQAQFQEM MADSMFVIPA LQVAHFQCSR APVYFYEFQH QPSWLKNIRP
460 470 480 490 500
PHMKADHGDE LPFVFRSFFG GNYIKFTEEE EQLSRKMMKY WANFARNGNP
510 520 530 540 550
NGEGLPHWPL FDQEEQYLQL NLQPAVGRAL KAHRLQFWKK ALPQKIQELE
EPEERHTEL
The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MTAQSRSPTTPTFPGPSQRTPLTPCPVQTPRLGKALIHCWTDPGQPLGEQQRVRRQRTETSEPTM
The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MTAQSRSPTTPTFPGPSQRTPLTPCPVQTPRLGKALIHCWTDPGQPLGEQQRVRRQRTETSEPTM
458-474: GDELPFVFRSFFGGNYI → V
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QLP1 | J3QLP1_HUMAN | Cocaine esterase | CES2 | 125 | Annotation score: | ||
J3QSC0 | J3QSC0_HUMAN | Cocaine esterase | CES2 | 201 | Annotation score: | ||
H3BUN5 | H3BUN5_HUMAN | Cocaine esterase | CES2 | 122 | Annotation score: | ||
J3QKN7 | J3QKN7_HUMAN | Cocaine esterase | CES2 | 21 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 – 10 | Missing AA sequence (PubMed:9169443).Curated | 9 | |
Sequence conflicti | 180 | G → S in BAF83171 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 239 | V → M in BAF83171 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 385 | F → S in CAD98009 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 519 | Q → R in CAD98009 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_018396 | 34 | R → W1 Publication | 1 | |
Natural variantiVAR_018397 | 206 | R → H1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059804 | 1 | M → MTAQSRSPTTPTFPGPSQRT PLTPCPVQTPRLGKALIHCW TDPGQPLGEQQRVRRQRTET SEPTM in isoform 3 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_010161 | 458 – 474 | GDELP…GGNYI → V in isoform 2 and isoform 4. 1 PublicationAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09616 mRNA Translation: CAA70831.1 D50579 mRNA Translation: BAA23606.1 U60553 mRNA Translation: AAB03611.1 AL713761 mRNA Translation: CAD28531.1 AK290482 mRNA Translation: BAF83171.1 BX538086 mRNA Translation: CAD98009.1 AY851164 Genomic DNA Translation: AAW29943.1 AC009084 Genomic DNA No translation available. CH471092 Genomic DNA Translation: EAW83058.1 CH471092 Genomic DNA Translation: EAW83059.1 BC032095 mRNA Translation: AAH32095.1 |
CCDSi | CCDS10825.1 [O00748-1] CCDS45507.1 [O00748-2] |
PIRi | JC5408 |
RefSeqi | NP_003860.2, NM_003869.5 [O00748-1] NP_932327.1, NM_198061.2 [O00748-2] |
UniGenei | Hs.282975 |
Genome annotation databases
Ensembli | ENST00000317091; ENSP00000317842; ENSG00000172831 [O00748-4] ENST00000417689; ENSP00000394452; ENSG00000172831 [O00748-5] |
GeneIDi | 8824 |
KEGGi | hsa:8824 |
UCSCi | uc002eqq.4 human [O00748-1] uc002eqr.4 human |
Keywords - Coding sequence diversityi
Alternative initiation, Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y09616 mRNA Translation: CAA70831.1 D50579 mRNA Translation: BAA23606.1 U60553 mRNA Translation: AAB03611.1 AL713761 mRNA Translation: CAD28531.1 AK290482 mRNA Translation: BAF83171.1 BX538086 mRNA Translation: CAD98009.1 AY851164 Genomic DNA Translation: AAW29943.1 AC009084 Genomic DNA No translation available. CH471092 Genomic DNA Translation: EAW83058.1 CH471092 Genomic DNA Translation: EAW83059.1 BC032095 mRNA Translation: AAH32095.1 |
CCDSi | CCDS10825.1 [O00748-1] CCDS45507.1 [O00748-2] |
PIRi | JC5408 |
RefSeqi | NP_003860.2, NM_003869.5 [O00748-1] NP_932327.1, NM_198061.2 [O00748-2] |
UniGenei | Hs.282975 |
3D structure databases
ProteinModelPortali | O00748 |
SMRi | O00748 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 114351, 1 interactor |
IntActi | O00748, 2 interactors |
STRINGi | 9606.ENSP00000317842 |
Chemistry databases
BindingDBi | O00748 |
ChEMBLi | CHEMBL3180 |
DrugBanki | DB06695 Dabigatran etexilate DB00762 Irinotecan DB00688 Mycophenolate mofetil DB06209 Prasugrel |
SwissLipidsi | SLP:000001424 |
Protein family/group databases
ESTHERi | human-CES2 Carb_B_Chordata |
MEROPSi | S09.984 |
PTM databases
iPTMneti | O00748 |
PhosphoSitePlusi | O00748 |
Polymorphism and mutation databases
BioMutai | CES2 |
Proteomic databases
EPDi | O00748 |
jPOSTi | O00748 |
MaxQBi | O00748 |
PaxDbi | O00748 |
PeptideAtlasi | O00748 |
PRIDEi | O00748 |
ProteomicsDBi | 48014 48015 [O00748-2] |
Protocols and materials databases
DNASUi | 8824 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000317091; ENSP00000317842; ENSG00000172831 [O00748-4] ENST00000417689; ENSP00000394452; ENSG00000172831 [O00748-5] |
GeneIDi | 8824 |
KEGGi | hsa:8824 |
UCSCi | uc002eqq.4 human [O00748-1] uc002eqr.4 human |
Organism-specific databases
CTDi | 8824 |
DisGeNETi | 8824 |
EuPathDBi | HostDB:ENSG00000172831.11 |
GeneCardsi | CES2 |
HGNCi | HGNC:1864 CES2 |
HPAi | HPA018897 |
MIMi | 605278 gene |
neXtProti | NX_O00748 |
OpenTargetsi | ENSG00000172831 |
PharmGKBi | PA377 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1516 Eukaryota COG2272 LUCA |
GeneTreei | ENSGT00940000153793 |
HOGENOMi | HOG000091866 |
HOVERGENi | HBG008839 |
InParanoidi | O00748 |
KOi | K03927 |
OMAi | GASAYWP |
OrthoDBi | 754103at2759 |
PhylomeDBi | O00748 |
TreeFami | TF315470 |
Enzyme and pathway databases
BRENDAi | 3.1.1.1 2681 3.1.1.84 2681 |
Reactomei | R-HSA-211945 Phase I - Functionalization of compounds |
SABIO-RKi | O00748 |
Miscellaneous databases
ChiTaRSi | CES2 human |
GeneWikii | Carboxylesterase_2 |
GenomeRNAii | 8824 |
PROi | PR:O00748 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000172831 Expressed in 236 organ(s), highest expression level in jejunal mucosa |
ExpressionAtlasi | O00748 baseline and differential |
Genevisiblei | O00748 HS |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058 AB_hydrolase IPR002018 CarbesteraseB IPR019826 Carboxylesterase_B_AS IPR019819 Carboxylesterase_B_CS |
Pfami | View protein in Pfam PF00135 COesterase, 1 hit |
SUPFAMi | SSF53474 SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122 CARBOXYLESTERASE_B_1, 1 hit PS00941 CARBOXYLESTERASE_B_2, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | EST2_HUMAN | |
Accessioni | O00748Primary (citable) accession number: O00748 Secondary accession number(s): A0A024R6X1 Q8TCP8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | July 1, 1997 | |
Last modified: | February 13, 2019 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations