Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor E2F3

Gene

E2F3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi155 – 245Sequence analysisAdd BLAST91

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1912408 Pre-NOTCH Transcription and Translation
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559585 Oncogene Induced Senescence
R-HSA-68689 CDC6 association with the ORC:origin complex
R-HSA-68911 G2 Phase
R-HSA-69231 Cyclin D associated events in G1
SignaLinkiO00716
SIGNORiO00716

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F3
Short name:
E2F-3
Gene namesi
Name:E2F3
Synonyms:KIAA0075
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112242.14
HGNCiHGNC:3115 E2F3
MIMi600427 gene
neXtProtiNX_O00716

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1871
OpenTargetsiENSG00000112242
PharmGKBiPA27573

Polymorphism and mutation databases

BioMutaiE2F3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194661 – 465Transcription factor E2F3Add BLAST465

Proteomic databases

EPDiO00716
MaxQBiO00716
PaxDbiO00716
PeptideAtlasiO00716
PRIDEiO00716
ProteomicsDBi48008

PTM databases

iPTMnetiO00716
PhosphoSitePlusiO00716

Expressioni

Gene expression databases

BgeeiENSG00000112242 Expressed in 215 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_E2F3
ExpressionAtlasiO00716 baseline and differential
GenevisibleiO00716 HS

Organism-specific databases

HPAiHPA029779
HPA065012

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108203, 36 interactors
DIPiDIP-24230N
ELMiO00716
IntActiO00716, 131 interactors
MINTiO00716
STRINGi9606.ENSP00000262904

Structurei

3D structure databases

ProteinModelPortaliO00716
SMRiO00716
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 153Cyclin A/CDK2 bindingSequence analysisAdd BLAST53
Regioni204 – 225Leucine-zipperAdd BLAST22
Regioni246 – 337DimerizationSequence analysisAdd BLAST92
Regioni391 – 465TransactivationSequence analysisAdd BLAST75
Regioni432 – 449Retinoblastoma protein bindingSequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi209 – 245DEF boxAdd BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 31Poly-Ala6
Compositional biasi45 – 53Poly-Ala9
Compositional biasi120 – 129Poly-Gly10

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiO00716
KOiK06620
OMAiLLCPDEE
OrthoDBiEOG091G087U
PhylomeDBiO00716
TreeFamiTF105566

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O00716-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA
60 70 80 90 100
AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY
110 120 130 140 150
TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL
160 170 180 190 200
KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG
210 220 230 240 250
VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG
260 270 280 290 300
GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT
310 320 330 340 350
YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL
360 370 380 390 400
CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL
410 420 430 440 450
ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA
460
YDLEKLPLVE DFMCS
Length:465
Mass (Da):49,162
Last modified:July 1, 1997 - v1
Checksum:i4641565842CA99EC
GO
Isoform 2 (identifier: O00716-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRKGIQ → MPLQQQ
     7-131: Missing.
     169-175: TPKSPSE → K

Note: No experimental confirmation available.
Show »
Length:334
Mass (Da):37,010
Checksum:i11CD170783B1974D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087X0B6A0A087X0B6_HUMAN
Transcription factor E2F3
E2F3
128Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014341344G → R1 PublicationCorresponds to variant dbSNP:rs4134973Ensembl.1
Natural variantiVAR_014342389D → N1 PublicationCorresponds to variant dbSNP:rs4134982Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0450661 – 6MRKGIQ → MPLQQQ in isoform 2. 1 Publication6
Alternative sequenceiVSP_0450677 – 131Missing in isoform 2. 1 PublicationAdd BLAST125
Alternative sequenceiVSP_045068169 – 175TPKSPSE → K in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10479 mRNA Translation: CAA71504.1
AF547386 Genomic DNA Translation: AAN17846.1
CR749285 mRNA Translation: CAH18140.1
AL132775 Genomic DNA No translation available.
AL136303 Genomic DNA No translation available.
D38550 mRNA Translation: BAA07553.1
CCDSiCCDS4545.1 [O00716-1]
CCDS58999.1 [O00716-2]
RefSeqiNP_001230005.1, NM_001243076.2 [O00716-2]
NP_001940.1, NM_001949.4 [O00716-1]
UniGeneiHs.269408
Hs.703174

Genome annotation databases

EnsembliENST00000346618; ENSP00000262904; ENSG00000112242 [O00716-1]
ENST00000535432; ENSP00000443418; ENSG00000112242 [O00716-2]
GeneIDi1871
KEGGihsa:1871
UCSCiuc003nda.3 human [O00716-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10479 mRNA Translation: CAA71504.1
AF547386 Genomic DNA Translation: AAN17846.1
CR749285 mRNA Translation: CAH18140.1
AL132775 Genomic DNA No translation available.
AL136303 Genomic DNA No translation available.
D38550 mRNA Translation: BAA07553.1
CCDSiCCDS4545.1 [O00716-1]
CCDS58999.1 [O00716-2]
RefSeqiNP_001230005.1, NM_001243076.2 [O00716-2]
NP_001940.1, NM_001949.4 [O00716-1]
UniGeneiHs.269408
Hs.703174

3D structure databases

ProteinModelPortaliO00716
SMRiO00716
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108203, 36 interactors
DIPiDIP-24230N
ELMiO00716
IntActiO00716, 131 interactors
MINTiO00716
STRINGi9606.ENSP00000262904

PTM databases

iPTMnetiO00716
PhosphoSitePlusiO00716

Polymorphism and mutation databases

BioMutaiE2F3

Proteomic databases

EPDiO00716
MaxQBiO00716
PaxDbiO00716
PeptideAtlasiO00716
PRIDEiO00716
ProteomicsDBi48008

Protocols and materials databases

DNASUi1871
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346618; ENSP00000262904; ENSG00000112242 [O00716-1]
ENST00000535432; ENSP00000443418; ENSG00000112242 [O00716-2]
GeneIDi1871
KEGGihsa:1871
UCSCiuc003nda.3 human [O00716-1]

Organism-specific databases

CTDi1871
DisGeNETi1871
EuPathDBiHostDB:ENSG00000112242.14
GeneCardsiE2F3
HGNCiHGNC:3115 E2F3
HPAiHPA029779
HPA065012
MIMi600427 gene
neXtProtiNX_O00716
OpenTargetsiENSG00000112242
PharmGKBiPA27573
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
GeneTreeiENSGT00550000074403
HOGENOMiHOG000232045
HOVERGENiHBG002227
InParanoidiO00716
KOiK06620
OMAiLLCPDEE
OrthoDBiEOG091G087U
PhylomeDBiO00716
TreeFamiTF105566

Enzyme and pathway databases

ReactomeiR-HSA-1912408 Pre-NOTCH Transcription and Translation
R-HSA-2559580 Oxidative Stress Induced Senescence
R-HSA-2559585 Oncogene Induced Senescence
R-HSA-68689 CDC6 association with the ORC:origin complex
R-HSA-68911 G2 Phase
R-HSA-69231 Cyclin D associated events in G1
SignaLinkiO00716
SIGNORiO00716

Miscellaneous databases

ChiTaRSiE2F3 human
GeneWikiiE2F3
GenomeRNAii1871
PROiPR:O00716
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112242 Expressed in 215 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_E2F3
ExpressionAtlasiO00716 baseline and differential
GenevisibleiO00716 HS

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE2F3_HUMAN
AccessioniPrimary (citable) accession number: O00716
Secondary accession number(s): Q15000, Q68DT0, Q9BZ44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 7, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again