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Protein

Ficolin-1

Gene

FCN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed:21037097). Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262CalciumCombined sources3 Publications1
Metal bindingi264CalciumCombined sources3 Publications1
Metal bindingi266Calcium; via carbonyl oxygenCombined sources3 Publications1
Metal bindingi268Calcium; via carbonyl oxygenCombined sources3 Publications1
Sitei300Mediates specificity for sialic acids2 Publications1
Sitei312Mediates specificity for sialic acids1 Publication1

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • G protein-coupled receptor binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: Reactome
  • sialic acid binding Source: UniProtKB
  • signaling pattern recognition receptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processImmunity, Innate immunity
LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-166662 Lectin pathway of complement activation
R-HSA-166663 Initial triggering of complement
R-HSA-2855086 Ficolins bind to repetitive carbohydrate structures on the target cell surface
R-HSA-6798695 Neutrophil degranulation

Protein family/group databases

UniLectiniO00602

Names & Taxonomyi

Protein namesi
Recommended name:
Ficolin-1
Alternative name(s):
Collagen/fibrinogen domain-containing protein 1
Ficolin-A
Ficolin-alpha
M-ficolin
Gene namesi
Name:FCN1
Synonyms:FCNM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000085265.10
HGNCiHGNC:3623 FCN1
MIMi601252 gene
neXtProtiNX_O00602

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi250G → F: Inhibits binding to the 9-O-acetylated sialic acid derivatives. 1 Publication1
Mutagenesisi285A → V: Inhibits binding to the 9-O-acetylated sialic acid derivatives. 1 Publication1
Mutagenesisi300Y → F: Abolishes interaction with all sialic acid-containing glycans. 1 Publication1

Organism-specific databases

DisGeNETi2219
OpenTargetsiENSG00000085265
PharmGKBiPA28069

Polymorphism and mutation databases

BioMutaiFCN1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000000913630 – 326Ficolin-1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi111 ↔ 139Combined sources2 Publications
Disulfide bondi118 ↔ 146Combined sources3 Publications
Disulfide bondi270 ↔ 283Combined sources3 Publications
Glycosylationi305N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO00602
PaxDbiO00602
PeptideAtlasiO00602
PRIDEiO00602
ProteomicsDBi47994

PTM databases

PhosphoSitePlusiO00602

Expressioni

Tissue specificityi

Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes.1 Publication

Gene expression databases

BgeeiENSG00000085265 Expressed in 122 organ(s), highest expression level in bone marrow
CleanExiHS_FCN1
ExpressionAtlasiO00602 baseline and differential
GenevisibleiO00602 HS

Organism-specific databases

HPAiCAB016760
HPA000685
HPA001295

Interactioni

Subunit structurei

Homotrimer (PubMed:17897951). Interacts with elastin/ELN. Interacts (via Fibrinogen C-terminal domain) with FFAR2. Interacts with CRP; may regulate monocyte activation by FCN1.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108513, 16 interactors
IntActiO00602, 3 interactors
MINTiO00602
STRINGi9606.ENSP00000360871

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO00602
SMRiO00602
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00602

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 93Collagen-likeAdd BLAST39
Domaini109 – 326Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 154A domain; contributes to trimerizationAdd BLAST40
Regioni155 – 243B domain; contributes to trimerizationAdd BLAST89
Regioni282 – 284Carbohydrate-binding1 Publication3
Regioni317 – 326P domain1 Publication10

Domaini

The fibrinogen C-terminal domain mediates calcium-dependent binding to carbohydrates and tethering to the cell surface in monocytes and granulocytes. The domain undergoes a conformational switch at pH under 6.2, and looses its carbohydrate-binding ability.2 Publications

Sequence similaritiesi

Belongs to the ficolin lectin family.Curated

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG2579 Eukaryota
ENOG410ZYS4 LUCA
GeneTreeiENSGT00930000150813
HOGENOMiHOG000037127
HOVERGENiHBG001644
InParanoidiO00602
KOiK10104
OMAiWYADCHA
PhylomeDBiO00602
TreeFamiTF329953

Family and domain databases

CDDicd00087 FReD, 1 hit
Gene3Di3.90.215.10, 1 hit
4.10.530.10, 1 hit
InterProiView protein in InterPro
IPR008160 Collagen
IPR036056 Fibrinogen-like_C
IPR014716 Fibrinogen_a/b/g_C_1
IPR014715 Fibrinogen_a/b/g_C_2
IPR002181 Fibrinogen_a/b/g_C_dom
IPR020837 Fibrinogen_CS
PfamiView protein in Pfam
PF01391 Collagen, 1 hit
PF00147 Fibrinogen_C, 1 hit
SMARTiView protein in SMART
SM00186 FBG, 1 hit
SUPFAMiSSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS00514 FIBRINOGEN_C_1, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O00602-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELSGATMAR GLAVLLVLFL HIKNLPAQAA DTCPEVKVVG LEGSDKLTIL
60 70 80 90 100
RGCPGLPGAP GPKGEAGVIG ERGERGLPGA PGKAGPVGPK GDRGEKGMRG
110 120 130 140 150
EKGDAGQSQS CATGPRNCKD LLDRGYFLSG WHTIYLPDCR PLTVLCDMDT
160 170 180 190 200
DGGGWTVFQR RMDGSVDFYR DWAAYKQGFG SQLGEFWLGN DNIHALTAQG
210 220 230 240 250
SSELRVDLVD FEGNHQFAKY KSFKVADEAE KYKLVLGAFV GGSAGNSLTG
260 270 280 290 300
HNNNFFSTKD QDNDVSSSNC AEKFQGAWWY ADCHASNLNG LYLMGPHESY
310 320
ANGINWSAAK GYKYSYKVSE MKVRPA
Length:326
Mass (Da):35,078
Last modified:May 2, 2002 - v2
Checksum:i184D24B371B251AB
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WVE2A0A087WVE2_HUMAN
Ficolin-1
FCN1
360Annotation score:

Sequence cautioni

The sequence BAA12120 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133T → N in AAB50706 (PubMed:8573080).Curated1
Sequence conflicti287N → S in AAB50706 (PubMed:8573080).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06117293R → Q. Corresponds to variant dbSNP:rs56345770Ensembl.1
Natural variantiVAR_024450126Y → H. Corresponds to variant dbSNP:rs771359747Ensembl.1
Natural variantiVAR_036341175Y → C in a colorectal cancer sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80990 mRNA Translation: AAB50706.1
AK314867 mRNA Translation: BAG37382.1
AL353611 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88137.1
BC020635 mRNA Translation: AAH20635.1
D83920 mRNA Translation: BAA12120.1 Different initiation.
CCDSiCCDS6985.1
PIRiS61517
RefSeqiNP_001994.2, NM_002003.4
UniGeneiHs.440898
Hs.638586

Genome annotation databases

EnsembliENST00000371806; ENSP00000360871; ENSG00000085265
GeneIDi2219
KEGGihsa:2219
UCSCiuc004cfi.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80990 mRNA Translation: AAB50706.1
AK314867 mRNA Translation: BAG37382.1
AL353611 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88137.1
BC020635 mRNA Translation: AAH20635.1
D83920 mRNA Translation: BAA12120.1 Different initiation.
CCDSiCCDS6985.1
PIRiS61517
RefSeqiNP_001994.2, NM_002003.4
UniGeneiHs.440898
Hs.638586

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D39X-ray1.90A/B/C115-326[»]
2JHHX-ray1.70C/F109-326[»]
2JHIX-ray1.80F109-326[»]
2JHKX-ray1.75F109-326[»]
2JHLX-ray1.75F109-326[»]
2JHMX-ray1.52F109-326[»]
2WNPX-ray1.21F110-326[»]
ProteinModelPortaliO00602
SMRiO00602
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108513, 16 interactors
IntActiO00602, 3 interactors
MINTiO00602
STRINGi9606.ENSP00000360871

Protein family/group databases

UniLectiniO00602

PTM databases

PhosphoSitePlusiO00602

Polymorphism and mutation databases

BioMutaiFCN1

Proteomic databases

EPDiO00602
PaxDbiO00602
PeptideAtlasiO00602
PRIDEiO00602
ProteomicsDBi47994

Protocols and materials databases

DNASUi2219
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371806; ENSP00000360871; ENSG00000085265
GeneIDi2219
KEGGihsa:2219
UCSCiuc004cfi.4 human

Organism-specific databases

CTDi2219
DisGeNETi2219
EuPathDBiHostDB:ENSG00000085265.10
GeneCardsiFCN1
HGNCiHGNC:3623 FCN1
HPAiCAB016760
HPA000685
HPA001295
MIMi601252 gene
neXtProtiNX_O00602
OpenTargetsiENSG00000085265
PharmGKBiPA28069
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2579 Eukaryota
ENOG410ZYS4 LUCA
GeneTreeiENSGT00930000150813
HOGENOMiHOG000037127
HOVERGENiHBG001644
InParanoidiO00602
KOiK10104
OMAiWYADCHA
PhylomeDBiO00602
TreeFamiTF329953

Enzyme and pathway databases

ReactomeiR-HSA-166662 Lectin pathway of complement activation
R-HSA-166663 Initial triggering of complement
R-HSA-2855086 Ficolins bind to repetitive carbohydrate structures on the target cell surface
R-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

EvolutionaryTraceiO00602
GeneWikiiFCN1
GenomeRNAii2219
PROiPR:O00602
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000085265 Expressed in 122 organ(s), highest expression level in bone marrow
CleanExiHS_FCN1
ExpressionAtlasiO00602 baseline and differential
GenevisibleiO00602 HS

Family and domain databases

CDDicd00087 FReD, 1 hit
Gene3Di3.90.215.10, 1 hit
4.10.530.10, 1 hit
InterProiView protein in InterPro
IPR008160 Collagen
IPR036056 Fibrinogen-like_C
IPR014716 Fibrinogen_a/b/g_C_1
IPR014715 Fibrinogen_a/b/g_C_2
IPR002181 Fibrinogen_a/b/g_C_dom
IPR020837 Fibrinogen_CS
PfamiView protein in Pfam
PF01391 Collagen, 1 hit
PF00147 Fibrinogen_C, 1 hit
SMARTiView protein in SMART
SM00186 FBG, 1 hit
SUPFAMiSSF56496 SSF56496, 1 hit
PROSITEiView protein in PROSITE
PS00514 FIBRINOGEN_C_1, 1 hit
PS51406 FIBRINOGEN_C_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFCN1_HUMAN
AccessioniPrimary (citable) accession number: O00602
Secondary accession number(s): Q5VYV5, Q92596
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 2, 2002
Last modified: November 7, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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